Bacterial Growth

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Bacteria divide by a process known as binary fission in which a single bacterium splits into two.

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  • Hi,

    I would like to ask how to simulate the formation of biofilm? I mean the most simply method and more to real application. Do you have suggestion how to do it?



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Bacterial Growth

  1. 1. Dr Maria Omair Registrar Microbiology Army Medical College
  2. 2. Training objectives <ul><li>Introduction to the topic </li></ul><ul><li>Understand exam scenarios </li></ul><ul><li>Prepare for viva voce </li></ul>
  3. 3. Bacterial Growth <ul><li>Bacteria divide by a Process called Binary Fission </li></ul>
  4. 4. Binary Fission <ul><li>Binary Fission is a type of Asexual reproduction in single celled organisms </li></ul><ul><li>Each cell divides into 2 equal sized offspring </li></ul>
  5. 5. Bacterial Doubling Time <ul><li>Escherichia coli 20 minutes </li></ul><ul><li>Mycobacterium tuberculosis 1 8 hours </li></ul><ul><li>Mycobacterium leprae 14 days </li></ul>
  6. 6. Logarithmic or Exponential Growth <ul><li>The population of bacterial cells divide at a constant rate so that the total number of cells doubles with each division </li></ul>
  7. 7. Bacteria Undergo Exponential Growth
  8. 8. Stages of Bacterial Growth <ul><li>Lag </li></ul><ul><li>Log (logarithmic) </li></ul><ul><li>Stationary </li></ul><ul><li>Death </li></ul>
  9. 9. Stages of Bacterial Growth <ul><li>Lag Adapt to nutrients </li></ul><ul><li>Log Active Growth </li></ul><ul><li>Stationary Growth equals death </li></ul><ul><li>Death Nutrients Consumed </li></ul>
  10. 10. Stages of Bacterial growth
  11. 12. Factors affecting Bacterial Growth <ul><li>Amount of nutrients </li></ul><ul><li>Temperature </li></ul><ul><li>pH </li></ul><ul><li>Other Environmental factors </li></ul>
  12. 13. Classification based upon Nutrient requirements <ul><li>Auxotrophs </li></ul><ul><ul><li>Can live on simple compounds </li></ul></ul><ul><ul><ul><li>NO 3 -, Glucose </li></ul></ul></ul><ul><ul><ul><li>Make all Amino Acids, Vitamins, etc. </li></ul></ul></ul><ul><li>Prototrophs </li></ul><ul><ul><li>Require complex compounds </li></ul></ul><ul><ul><ul><li>Vitamins, Amino Acids </li></ul></ul></ul><ul><ul><ul><li>Fastidious </li></ul></ul></ul><ul><ul><ul><li>Most Pathogens </li></ul></ul></ul>
  13. 14. Classification based upon temperature requirements <ul><li>Mesophiles </li></ul><ul><ul><li>Organisms which are able to grow at 20-40 0 C </li></ul></ul><ul><ul><li>Most of the human pathogens </li></ul></ul><ul><li>Psychrophiles </li></ul><ul><ul><li>Organisms which grow best at <10 0 C </li></ul></ul><ul><ul><li>Capable of growth in pharmaceuticals and foods stored in refrigerator </li></ul></ul><ul><li>Thermophiles </li></ul><ul><ul><li>Organisms which grow best at >6O 0 C </li></ul></ul>
  14. 15. Effect of Temp on Growth
  15. 16. Classification based upon pH requirements <ul><li>Acidophiles(pH <5.0) </li></ul><ul><ul><li>Organisms that grow at a low pH e.g. Lactobacillus spp., Thiobacillus ferroxidans </li></ul></ul><ul><li>Neutrophiles (pH 6.0-8.0) </li></ul><ul><ul><li>Organisms that grow at a neutral pH </li></ul></ul><ul><ul><li>Most of human pathogens </li></ul></ul><ul><li>Alkaliphiles (pH 8.5-10.5) </li></ul><ul><ul><li>Organisms that grow at a high salt conc. or at high pH e.g. Vibrio spp., Bacillus alcalophilus </li></ul></ul>
  16. 17. Effect of pH on Bacterial Growth
  17. 18. Classification based upon Oxygen requirements <ul><li>Strict Aerobes </li></ul><ul><ul><li>Grow only in the presence of ambient oxygen (21%) </li></ul></ul><ul><ul><li>e.g. Mycobacterium tuberculosis, Pseudomonas aeruginosa </li></ul></ul><ul><li>Strict Anaerobes </li></ul><ul><ul><li>Grow only in the absence of oxygen </li></ul></ul><ul><ul><li>Bacteroides fragilis </li></ul></ul>
  18. 19. Classification based upon Oxygen requirements (Contd.) <ul><li>Facultative Anaerobes </li></ul><ul><ul><li>Can grow in anaerobic environment but grow best in aerobic environment </li></ul></ul><ul><ul><li>e.g. Staphylococcus aureus, Escherichia coli </li></ul></ul><ul><li>Aerotolerant anaerobes </li></ul><ul><ul><li>Are anaerobes but can tolerate exposure to oxygen </li></ul></ul><ul><ul><li>e.g. Clostridium perfringens </li></ul></ul>
  19. 20. Classification based upon Oxygen requirements (Contd.) <ul><li>Microaerophilic </li></ul><ul><ul><li>Require or prefer decreased oxygen environment (5%) </li></ul></ul><ul><ul><li>e.g. Campylobacter jejuni , Helicobacter pylori </li></ul></ul><ul><li>Capnophilic </li></ul><ul><ul><li>Require or prefer increased CO2 environment </li></ul></ul><ul><ul><li>e.g. Neisseria meningitidis </li></ul></ul>
  20. 21. Effect of Oxygen Concentration on Bacterial Growth
  21. 24. Questions?

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