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I need help creating a flow chart for my unknown bacteria which was (P aeroginosa) Create a
flow chart of biochemical tests with the following bacteria to get P aeroginosa as your final
result. The other bacterias are: S sonnei S Typhi K pneumonia P aeroginosa (my unknown)
Solution
Identification of P.aeruginosa:
Laboratory identification:
Gram stain: Gram negative
Motility: Motile
Oxidase: Positive
Catalase: Positive
So, it belongs to non- eneterobacteriaceae species such as Vibrio and Pseudomonas species.
MacConkey’s agar: Non-lactose fermenting
Growth on nutrient agar: Produces non-fluorescent bluish green colonies due to the presence of
characteristic toxin released by P. aeruginosa, “PYOCYANIN.” Vibrio do not produce this
pigment.
Causes beta hemolysis on blood agar media. The colonies best grow at 420C and produces sweet
odour.

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I need help creating a flow chart for my unknown bacteria which was .pdf

  • 1. I need help creating a flow chart for my unknown bacteria which was (P aeroginosa) Create a flow chart of biochemical tests with the following bacteria to get P aeroginosa as your final result. The other bacterias are: S sonnei S Typhi K pneumonia P aeroginosa (my unknown) Solution Identification of P.aeruginosa: Laboratory identification: Gram stain: Gram negative Motility: Motile Oxidase: Positive Catalase: Positive So, it belongs to non- eneterobacteriaceae species such as Vibrio and Pseudomonas species. MacConkey’s agar: Non-lactose fermenting Growth on nutrient agar: Produces non-fluorescent bluish green colonies due to the presence of characteristic toxin released by P. aeruginosa, “PYOCYANIN.” Vibrio do not produce this pigment. Causes beta hemolysis on blood agar media. The colonies best grow at 420C and produces sweet odour.