ubio sensit Filariasis Antibody rapid test


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The ubio sensit Filaria antibody Rapid Test is a lateral flow immunoassay for
detection of anti-lymphatic filarial parasites (W. Bancrofti and B. Malayi) in human whole blood, plasma or serum. This test is intended to be used as a screening test and as an aid in the diagnosis of infection with lymphatic filarial parasites.

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ubio sensit Filariasis Antibody rapid test

  1. 1. Diagnosis of HumanFilariasisubio sensitFilariasisAntibodyTest
  2. 2. lymphatic filariasis  Long known disease which only in a minority of cases results in severe elephantiasis  120 million people infected of which 40 million show symptoms (1996)  Caused by three species of filaria: Wucheria bancrofti, Brugia malayi, B. timori
  3. 3. Lymphatic Filariasis: Life Cycle
  4. 4. Filaria and filariasisTissue dwelling nematodes with adults and L1 present in the human hostArthropode vector which takes up L1 and transmits L3Onchocerciasis, Lymphatic filariasis, (Loiasis)
  5. 5. Are endosymbionts involved in filarialpathogenesis?  Wolbachia bacteria are found as intracellular endosymbionts in the hypodermal lateral chord and the uterus of many parasitic nematodes (here Brugia malayi)  The bacteria seem important for nematode development as antibiotic treatment results in sterility (and maybe even death) of the adults
  6. 6. Are endosymbionts involved in filarialpathogenesis?  Sections through onchocercomas in untreated (upper) and antibiotic treated patients (11 months after a 6 weeks course of doxycyclin)  Note that Wolbachia detected by red stain are absent in the treated patient  In addition the treated worms show no healthy eggs or embryos  This suggest that antibiotics might provide new therapeutic approaches for the treatment of filariasis  There are also studies that suggest that the bacterial antigens might be responsible for the strong inflammation and hence pathology
  7. 7. lymphatic filariasisWucheria bancrofti Brugia malayi
  8. 8. lymphatic filariasis  Adult worms (macrofilariae) live in the lymphatic vessels and lymph nodes of the lower body half  Females are ovoviviparous producing L1 larvae still in the egg membrane (the sheath)
  9. 9. lymphatic filariasis  L1 lavae or microfilariae are swept into the blood stream and circulate  W.b. microfilariae are sheated and the nuclei do not reach the tip of the tail  Microfilariae remain viable and infective for several months
  10. 10. lymphatic filariasis  Microfilaria show diurnal rhythm  Wucheria and Brugia microfilariae are found in the peripheral blood during night time whereas Loa is found during the day hours  This is phenomenon is not linked to egg production but to the behavior of the MF  The benefit (of absence from the peripheral blood during the day is unclear)
  11. 11. lymphatic filariasis  Mircofilariae are taken up by mosquitoes with the blood meal  Broad spectrum of night active vectors (Culex, Aedes, Anopheles, Mansonia)  Microfilaria leave sheath in the mosquito midgut, penetrate the Culex quiquefasciatus midgut wall and migrate to the flight muscle where they molt twice (the develop intra- cellularly) W. bancrofti L1 in mosquito flight muscle
  12. 12. lymphatic filariasis  L3 migrate through hemolymph until they find the labium, which they penetrate when they sense that the mosquito is feeding  They move onto the skin and into the wound puncture  Development in mosquito takes about two weeks
  13. 13. lymphatic filariasis  Maturing larvae and adults provoke strong inflammatory reaction  Acute symptoms are painful lymphnode and lymphchannel inflammation and swelling which is often accompanied by fever  Brugia infection is very similar to Wucheria  Acute reactions are more pronounced (e.g. formation of abscesses)  Elephantiasis tends to affect arms instead of legs
  14. 14. lymphatic filariasis  Progessive chronic disease can lead to wide spread fibrosis and damage of lymphatic vessels, which can result in rupture and discharge of lymph into the urinary system (chyluria) or the scrotum
  15. 15. lymphatic filariasis  In men chronic infection often results in hydrocele, a painful swelling of the scrotum due to blockage and inflammation of the adjacent draining lymph vessels  Up to 20% of all grown man in certain communities in Haiti suffer from hydrocele  No effects on fertility, but wide spread sexual disability  Profound effects on patients self esteem and family life
  16. 16. lymphatic filariasis Adult filaria can be detected in the scrotal lymph vessels of men with hydrocele by ultra sound Macrofilaria can be identified easily by there mobility
  17. 17. lymphatic filariasis  Chronic disease has complex inflammatory etiology  Dieing filaria are especially potent in triggering inflammation, and death of worms if followed by episodes of fever, pain and acute inflammation  Recent work suggests that, in addition to adult worms, secondary bacterial and fungal infections play an important role in acute episodes and chronic progression of the disease
  18. 18. lymphatic filariasis Only a minority of patients progresses to elephantiasis and the factors contributing to their susceptibility are not well understood©WHO-TDR
  19. 19. lymphatic filariasis Diagnosis:  Demonstration of microfilaria in blood, or lymph (has to be done at night!)  Antibody and antigen capture assays (dip stick format)  Demonstration of adult worms by ultra sound
  20. 20. lymphatic filariasis Oedema of arm and hand in this patient with filariasis Lesions between fingers and toes are especially vulnerable to bacterial infection
  21. 21. lymphatic filariasis  Chemotherapy can be used to kill microfilaria but does not affect adult worms (antibiotic treatment targeting Wolbachia may hold promise here)  However strict antiseptic regimens using soap and antibacterial ointments can greatly reduce swelling, pain and other symptoms and even revert many severe symptoms
  22. 22. sensitFilariasisAntibody test
  23. 23. ubio’s solution for FilariasisDiagnosis One step Immuno-chromatographic test for Filarial antibodies . Whole blood or serum Uses a novel recombinant filarial antigen. Results in 10 minutes. Filariasis can be detected in early stage of infection.
  24. 24. TEST PRINCIPLE  The ubio sensit Filaria antibody Rapid Test is a lateral flow chromatographic immunoassay.  The test cassette consists of 1) a pink colored conjugate pad containing Anti Human IgG conjugated with colloid gold, 2) a nitrocellulose membrane strip containing test band (T) and a control band (C). The T band is precoated with recombinant Filaria Antigen for the detection of anti-Filaria antibody.  Test specimen, with adequate amount of buffer, migrates along the conjugate pad and further across the coated membrane by capillary action.  Anti-Filarial antibodies present in the sample, complex with the Anti Human IgG present in the conjugate pad and gets captured onto the coated Filaria antigen. Thus giving a colored test band, indicating a W. bancrofti or B. malayi positive test result.  If the respective antibody is not present in the sample, no reaction occurs and no test line is formed.  The sample then migrates further along the strip until it reaches the control band, where excess Anti Human IgG - colloidal gold gets bound and produces a second visible line on the membrane. This control line indicates that the sample has migrated across the membrane as intended
  25. 25. Test Procedure Take out the test card from the foil pouch and place it on a horizontal surface. Add 10 μl of the specimen to the sample hole (marked S) on the test card. When the sample is fully absorbed, add three drops of the diluents provided with the assay Wait for 10-15 minutes and interpret results. The result is considered invalid after 20 minutes. All results where control band does not appear are considered invalid.
  26. 26. Test Performance 100 % specificity, (in endemic and non endemic normal) no cross reaction with Leishmania, malaria and other nematode parasites. >97% Sensitivity.
  27. 27. About ubio Leading IVD manufacturer from India ISO 13485 and GMP certified Manufacturing Facility. Focus on Neglected Tropical Diseases. Products include: Malaria pf, Malaria Pv, Malaria pf/Pan, Malaria Pf/Pv, TB MPT 64, TB LAM, Leishmania, Filaria, Leptospira IgM, Salmonella IgM, Brucella IgG, H.Pylori Ab/Ag, hCG and LH tests Veterinary Diagnostics. Leading uncut sheet supplier. OEM and Bulk manufacture.
  28. 28. For further details and placing your orders ubio Biotechnology Systems Pvt Ltd., XII-111E/F, Biotechnology Incubation Center, KINFRA Hi-tech Park, Kalamassery, Cochin – 683503, Kerala, India Ph: +91-484-2532966, +91-9744122269 www.ubio.in contact@ubio.in