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sensit
   TB MPT 64
Antigen Rapid test


         ubio Biotechnology Systems Pvt Ltd, Cochin

                 © 2008 ubio. All rights reserved.    1
TB
 one-third of the world's population is currently infected with
  the TB bacillus
 An estimated 1.7 million people died from TB in 2009(WHO)
 Africa and Asia account for 65% of total TB cases
 3.2% of the world's estimated new tuberculosis cases were
  MDR-TB,
 In India 3% of New TB cases are MDR TB
 MDR-TB cases in India represent over 20% of the global
  burden of MDR-TB, with an estimated 131,000 cases in 2007
 WHO estimates over 500,000 cases of multidrug-resistant
  tuberculosis (MDR-TB) occur annually worldwide



                            © 2008 ubio. All rights reserved.      2
TB Diagnostics
 Antibody Screening tests are discontinued due to poor reliability.

 Currently available tests include

   • Sputum Microscopy
   • Tuberculin test
   • Interferon Gamma Assay
   • PCR
   • TB antigen tests


                                 © 2008 ubio. All rights reserved.     3
TB Antigen rapid Tests


MPT 64
TB LAM Test
          © 2008 ubio. All rights reserved.   4
About MPT64
 The MPT64 antigen is the secretory
  protein (24 kDa) that is one of the
  secreted major antigens from
  tuberculosis bacteria,
 MPT64 has been shown to differentiate
  the MTC from other bacterial species



                 © 2008 ubio. All rights reserved.   5
Advantages of MPT 64 test
Differentiation between M.
 tuberculosis and NTM
Results in 20 minutes.
No need of PCR or Biochemical tests.
Results as early as 2-3 weeks of
 culture.
Low cost and no need of equipments

                © 2008 ubio. All rights reserved.   6
MPT 64 Rapid test
 Sensit TB Ag MPT64 Rapid Test Kit is a
  qualitative Immunochromatographic
  assay for the detection M. tuberculosis
  complex in liquid and solid culture




                  © 2008 ubio. All rights reserved.   7
Test Principle
 Sensit TB Ag MPT64 Rapid Test works on chromatographic
  immunoassay. B
 Basic components of test strip includes:
    a)    Conjugate pad, which contains Monoclonal Anti-MPT64 antibody,
          colloidal gold conjugated;
    b) a nitrocellulose membrane strip containing two lines
         a)   T: Monoclonal Anti-MPT64 antibody and
         b) C: Goat Anti Mouse.




                                © 2008 ubio. All rights reserved.         8
Sample Preparation
 Liquid Cultures: One drop of liquid culture can
  be directly added to the sample well of the test
  device.
 If the culture is too turbid, adjust the opacity
  with McFarland No 1 using PBS.
 · Solid cultures: Pick 3-4 colonies from solid
  culture and suspend in 200 ul of Assay buffer.
 Treat the specimen as infectious and handle with standard
  biosafety measures.


                           © 2008 ubio. All rights reserved.   9
Test Procedure
 Take out the test card from the foil pouch and place it
  on a horizontal surface.
 Add 25 ul (using pipette)/one drop (using dropper
  provided) of the liquid or solid culture (processed as
  above) to the sample well, followed by the addition of
  one drop of assay diluent.
 Wait for 20 minutes and interpret results.
 The result is considered invalid after 30 minutes.
 All results where control band does not appear are
  considered invalid.


                        © 2008 ubio. All rights reserved.   10
Test Result Interpretation




          © 2008 ubio. All rights reserved.   11
For further details and Ordering

ubio Biotechnology Systems Pvt Ltd
XII-111-E/F, Technology Incubation Centre
KINFRA Hi-Tech Park, Kalamassery
Cochin, Kerala, India 683503
Ph: +91-9744122269, +91-484-2532966
http://www.ubio.in




                                © 2008 ubio. All rights reserved.   12
www.ubio.in
contact@ubio.in




   © 2008 ubio. All rights reserved.   13

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Sensit MPT 64 TB Antigen rapid test

  • 1. sensit TB MPT 64 Antigen Rapid test ubio Biotechnology Systems Pvt Ltd, Cochin © 2008 ubio. All rights reserved. 1
  • 2. TB  one-third of the world's population is currently infected with the TB bacillus  An estimated 1.7 million people died from TB in 2009(WHO)  Africa and Asia account for 65% of total TB cases  3.2% of the world's estimated new tuberculosis cases were MDR-TB,  In India 3% of New TB cases are MDR TB  MDR-TB cases in India represent over 20% of the global burden of MDR-TB, with an estimated 131,000 cases in 2007  WHO estimates over 500,000 cases of multidrug-resistant tuberculosis (MDR-TB) occur annually worldwide © 2008 ubio. All rights reserved. 2
  • 3. TB Diagnostics  Antibody Screening tests are discontinued due to poor reliability.  Currently available tests include • Sputum Microscopy • Tuberculin test • Interferon Gamma Assay • PCR • TB antigen tests © 2008 ubio. All rights reserved. 3
  • 4. TB Antigen rapid Tests MPT 64 TB LAM Test © 2008 ubio. All rights reserved. 4
  • 5. About MPT64  The MPT64 antigen is the secretory protein (24 kDa) that is one of the secreted major antigens from tuberculosis bacteria,  MPT64 has been shown to differentiate the MTC from other bacterial species © 2008 ubio. All rights reserved. 5
  • 6. Advantages of MPT 64 test Differentiation between M. tuberculosis and NTM Results in 20 minutes. No need of PCR or Biochemical tests. Results as early as 2-3 weeks of culture. Low cost and no need of equipments © 2008 ubio. All rights reserved. 6
  • 7. MPT 64 Rapid test  Sensit TB Ag MPT64 Rapid Test Kit is a qualitative Immunochromatographic assay for the detection M. tuberculosis complex in liquid and solid culture © 2008 ubio. All rights reserved. 7
  • 8. Test Principle  Sensit TB Ag MPT64 Rapid Test works on chromatographic immunoassay. B  Basic components of test strip includes: a) Conjugate pad, which contains Monoclonal Anti-MPT64 antibody, colloidal gold conjugated; b) a nitrocellulose membrane strip containing two lines a) T: Monoclonal Anti-MPT64 antibody and b) C: Goat Anti Mouse. © 2008 ubio. All rights reserved. 8
  • 9. Sample Preparation  Liquid Cultures: One drop of liquid culture can be directly added to the sample well of the test device.  If the culture is too turbid, adjust the opacity with McFarland No 1 using PBS.  · Solid cultures: Pick 3-4 colonies from solid culture and suspend in 200 ul of Assay buffer.  Treat the specimen as infectious and handle with standard biosafety measures. © 2008 ubio. All rights reserved. 9
  • 10. Test Procedure  Take out the test card from the foil pouch and place it on a horizontal surface.  Add 25 ul (using pipette)/one drop (using dropper provided) of the liquid or solid culture (processed as above) to the sample well, followed by the addition of one drop of assay diluent.  Wait for 20 minutes and interpret results.  The result is considered invalid after 30 minutes.  All results where control band does not appear are considered invalid. © 2008 ubio. All rights reserved. 10
  • 11. Test Result Interpretation © 2008 ubio. All rights reserved. 11
  • 12. For further details and Ordering ubio Biotechnology Systems Pvt Ltd XII-111-E/F, Technology Incubation Centre KINFRA Hi-Tech Park, Kalamassery Cochin, Kerala, India 683503 Ph: +91-9744122269, +91-484-2532966 http://www.ubio.in © 2008 ubio. All rights reserved. 12
  • 13. www.ubio.in contact@ubio.in © 2008 ubio. All rights reserved. 13