Synthesis of the silver nanoparticles from Aloe barbadensis extract and its a...
Virus Inactivation By Biogenic Silver
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2. Bart De Gusseme Virus inactivation by biogenic silver IBS 2010, RImini, September 15th, 2010
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6. Biogenic silver nanoparticles 0,5 µm 1,5 µm Biogenic metalic silver nanoparticles (bio-Ag 0 ) Reduction of Ag + on bacterial cell surface of dead Lactobacillus sp pH = 12 Ag + Ag + Ag + Ag + Ag + Ag +
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9. Disinfection assay on UZ1 (Contaminated drinking water, C 0 = 6.0 x 10 6 pfu mL -1 ) Bart De Gusseme Virus inactivation by biogenic silver IBS 2010, RImini, September 15th, 2010 > 4.2 log inactivation after 3 h with addition of Bio-Ag 0 No sorption to biomass, slightly faster than Ag + LOD
10. Disinfection assay on MNV-1 (C 0 = 1.1 x 10 6 pfu mL -1 , 8.9 x 10 8 gc mL -1 ) Bart De Gusseme Virus inactivation by biogenic silver IBS 2010, RImini, September 15th, 2010 > 4.7 log inactivation after 30 min with addition of Bio-Ag 0 No removal detected with real-time RT-PCR : RNA intact LOD non-infectious RNA still intact
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14. Continuous virus removal Bart De Gusseme Virus inactivation by biogenic silver IBS 2010, RImini, September 15th, 2010 Only 1.5 log decrease of UZ1 after 10 HRTs without Bio-Ag 0 > 3.5 log decrease with addition of Bio-Ag 0
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18. Acknowledgements Bart De Gusseme (Aspirant), Tom Hennebel (project no. 7741-02) and Leen Baert (Postdoctoral fellow) are supported by the Fund of Scientific Research (FWO)-Flanders. Liesje Sintubin is supported by a project grant (no. 71333) of the Institute for the Promotion of Innovation through Science and Technology in Flanders (IWT). We gratefully thank Lieve Herman and Els Van Coillie of the Institute for Agricultural and Fisheries Research, Technology and Food Unit (Belgium) for the ability to use their laboratory facilities. Bart De Gusseme Virus inactivation by biogenic silver IBS 2010, RImini, September 15th, 2010