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14th International Biotechnology Symposium and Exhibition 2010 Rimini, Italy, September 15 th  2010 Bart De Gusseme L. Sintubin, L. Baert, T. Hennebel, M. Uyttendaele,  W. Verstraete and N. Boon Laboratory of Microbial Ecology & Technology, Ghent University, Belgium ,[object Object],[object Object],[object Object]
Bart De Gusseme  Virus inactivation by biogenic silver  IBS 2010, RImini, September 15th, 2010
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Introduction: waterborne diseases
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Conventional water disinfection Need for alternative techniques for drinking water disinfection Bart De Gusseme  Virus inactivation by biogenic silver  IBS 2010, RImini, September 15th, 2010
Nanoscale silver as disinfectant ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Bart De Gusseme  Virus inactivation by biogenic silver  IBS 2010, RImini, September 15th, 2010
Biogenic silver nanoparticles 0,5 µm 1,5 µm Biogenic metalic silver nanoparticles (bio-Ag 0 ) Reduction of Ag +  on bacterial cell surface of dead  Lactobacillus  sp pH = 12 Ag + Ag + Ag + Ag + Ag + Ag +
Biogenic silver nanoparticles ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Bart De Gusseme  Virus inactivation by biogenic silver  IBS 2010, RImini, September 15th, 2010 Bio-Ag 0  = nanoscale Ag 0  on a microscale bacterial carrier
Model organisms ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Bart De Gusseme  Virus inactivation by biogenic silver  IBS 2010, RImini, September 15th, 2010
Disinfection assay on UZ1 (Contaminated drinking water, C 0  = 6.0 x 10 6  pfu mL -1 ) Bart De Gusseme  Virus inactivation by biogenic silver  IBS 2010, RImini, September 15th, 2010 > 4.2 log inactivation after 3 h with addition of Bio-Ag 0 No sorption to biomass, slightly faster than Ag + LOD
Disinfection assay on MNV-1 (C 0  = 1.1 x 10 6  pfu mL -1 , 8.9 x 10 8  gc mL -1 ) Bart De Gusseme  Virus inactivation by biogenic silver  IBS 2010, RImini, September 15th, 2010 > 4.7 log inactivation after 30 min with addition of Bio-Ag 0 No removal detected with real-time RT-PCR : RNA intact LOD non-infectious RNA still intact
Inactivation mechanism (MNV-1) ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Bart De Gusseme  Virus inactivation by biogenic silver  IBS 2010, RImini, September 15th, 2010 Most probable inactivation mechanism: Interaction of Ag 0  with (thiol groups of the) protein capsid, preventing the virus from docking onto his host cell
Challenge ,[object Object],[object Object],[object Object],[object Object],[object Object],Bart De Gusseme  Virus inactivation by biogenic silver  IBS 2010, RImini, September 15th, 2010 ,[object Object],[object Object]
NanoCeram ®  filter ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Bart De Gusseme  Virus inactivation by biogenic silver  IBS 2010, RImini, September 15th, 2010
Continuous virus removal Bart De Gusseme  Virus inactivation by biogenic silver  IBS 2010, RImini, September 15th, 2010 Only 1.5 log decrease of UZ1 after 10 HRTs without Bio-Ag 0 > 3.5 log decrease with addition of  Bio-Ag 0
Conclusions ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Bart De Gusseme  Virus inactivation by biogenic silver  IBS 2010, RImini, September 15th, 2010
Future perspectives ,[object Object],Bart De Gusseme  Virus inactivation by biogenic silver  IBS 2010, RImini, September 15th, 2010 Bio-Ag 0 Bio- Ag 0
Future perspectives ,[object Object],[object Object],[object Object],[object Object],Bart De Gusseme  Virus inactivation by biogenic silver  IBS 2010, RImini, September 15th, 2010
Acknowledgements Bart De Gusseme (Aspirant), Tom Hennebel (project no. 7741-02) and Leen Baert (Postdoctoral fellow) are supported by the  Fund of Scientific Research (FWO)-Flanders. Liesje Sintubin is supported by a project grant  (no. 71333) of the Institute for the Promotion of Innovation through Science and Technology in Flanders (IWT). We gratefully thank Lieve Herman and Els Van Coillie of the Institute for Agricultural and Fisheries Research, Technology and Food Unit (Belgium) for the ability to use their laboratory facilities. Bart De Gusseme  Virus inactivation by biogenic silver  IBS 2010, RImini, September 15th, 2010
B. De Gusseme, L. Sintubin, L. Baert, E. Thibo, T. Hennebel, G. Vermeulen, M. Uyttendaele, W. Verstraete & N. Boon ,[object Object],[object Object],[email_address] LabMET.UGent.be

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Virus Inactivation By Biogenic Silver

  • 1.
  • 2. Bart De Gusseme Virus inactivation by biogenic silver IBS 2010, RImini, September 15th, 2010
  • 3.
  • 4.
  • 5.
  • 6. Biogenic silver nanoparticles 0,5 µm 1,5 µm Biogenic metalic silver nanoparticles (bio-Ag 0 ) Reduction of Ag + on bacterial cell surface of dead Lactobacillus sp pH = 12 Ag + Ag + Ag + Ag + Ag + Ag +
  • 7.
  • 8.
  • 9. Disinfection assay on UZ1 (Contaminated drinking water, C 0 = 6.0 x 10 6 pfu mL -1 ) Bart De Gusseme Virus inactivation by biogenic silver IBS 2010, RImini, September 15th, 2010 > 4.2 log inactivation after 3 h with addition of Bio-Ag 0 No sorption to biomass, slightly faster than Ag + LOD
  • 10. Disinfection assay on MNV-1 (C 0 = 1.1 x 10 6 pfu mL -1 , 8.9 x 10 8 gc mL -1 ) Bart De Gusseme Virus inactivation by biogenic silver IBS 2010, RImini, September 15th, 2010 > 4.7 log inactivation after 30 min with addition of Bio-Ag 0 No removal detected with real-time RT-PCR : RNA intact LOD non-infectious RNA still intact
  • 11.
  • 12.
  • 13.
  • 14. Continuous virus removal Bart De Gusseme Virus inactivation by biogenic silver IBS 2010, RImini, September 15th, 2010 Only 1.5 log decrease of UZ1 after 10 HRTs without Bio-Ag 0 > 3.5 log decrease with addition of Bio-Ag 0
  • 15.
  • 16.
  • 17.
  • 18. Acknowledgements Bart De Gusseme (Aspirant), Tom Hennebel (project no. 7741-02) and Leen Baert (Postdoctoral fellow) are supported by the Fund of Scientific Research (FWO)-Flanders. Liesje Sintubin is supported by a project grant (no. 71333) of the Institute for the Promotion of Innovation through Science and Technology in Flanders (IWT). We gratefully thank Lieve Herman and Els Van Coillie of the Institute for Agricultural and Fisheries Research, Technology and Food Unit (Belgium) for the ability to use their laboratory facilities. Bart De Gusseme Virus inactivation by biogenic silver IBS 2010, RImini, September 15th, 2010
  • 19.