Josh Adams's Presentation on Bio-based Products
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Alternative forest products.
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Josh Adams's Presentation on Bio-based Products
- 1. Innovation of “Novel” Bio-based Products for Arkansas Joshua P. Adams and Danielle Julie Carrier
- 6. Sweetgum bark • Tree logs used in Kraft pulp mill are debarked before usage • Bark represents 12% of a tree biomass • Bark is being burned for energy • However, some bark tree (sweetgum and pine) contains extractives with antimicrobial activity Debarker from Evergreen Packaging Mill in Pine Bluff, AR Sweetgum bark and composition 29.76% 31.57% 16.96% 5.76% 15.95% Ash Others Lignin
- 8. Hot water extraction of sweetgum bark 1 L Parr reactor, set at 85°C for 60 min, with stirring 25 g sweetgum bark + 250 mL water Sweetgum bark hot water extractives
- 9. Testing extractives for antimicrobial activity One gram-positive pathogen Staphylococcus aureus Three gram-negative pathogens Salmonella typhimurium Listeria monocytogenes Escherichia coli Using disc diffusion assay with different concentration of extract
- 10. Antimicrobial activity on Staphylococcus aureus 10% Streptomycin (control) Sweetgum bark extract Zone of inhibition is a clear zone were antimicrobials are preventing bacteria from growing (control 23 mm zone, bark extract 11 mm zone)
- 11. Sweetgum extract as antioxidant 6.25 mg/mL inhibits oxidation of low density lipoprotein (LDL = bad cholesterol) standards 0 h 24 h 0 h 24 h 0 3.1 6.25 12.5 25 50
- 12. Chromatograph of certain compounds from sweetgum bark extract
- 18. A. B. C. D.
- 19. • 25 g pine needles in 250 mL 20% NaCl in water
- 20. Gas Chromatograph Mass Spectrometry of pine essential oil GC/MS of Essential Oils of Pine Needles α pinene β pinene limonene terpineol caryophyllene β caryophyllene
- 21. Compounds as a function of season 45 April 40 35 30 25 20 15 10 5 0 May June July September
- 23. Preparing Disc Diffusion test Staphylococcus aureus, including strains resistant to Methicillin Resistant (MRSA)
- 24. Zone of inhibition with Loblolly essential oil.
- 25. Conclusions Sweetgum • Could fit seamlessly into mill operations • Extract has antimicrobial activity against Staphylococcus aureus • Extract has antioxidant characteristics Pine • Needles contain essential oils • Mass of essential oil varied as a function of season • Essential oil showed activity against the methicillin resistant Staphylococcus aureus strain (MRSA)
- 26. Future work • Identify which compounds confer antimicrobial activity • Where are they located o Time o Age o Genotype • Integration
- 27. USDA Small Business Innovation Research (SBIR) programs.
- 28. Team Members
- 29. SFTIC June 8-10, 2015 Hot Springs
- 30. Questions?
Editor's Notes
- I put novel in quotes and will explain to the crowd how we are really rediscovering things that have been used for a long time.
- Ill talk about how sweetgum is a common nuisance that may have some advantageous qualities that are not taken advantage of.
- Here Ill highlight how a problem in pine sometimes is that they are very thick as in this picture and we actually have to pay someone to harvest them. Both this slide and the previous will be used to beg the question “Can we make these suboptimal scenarios profitable?”
- Ill then tell the story of some of the recommendations for common species highlighting sweetgum and pine.
- Picture of sweetgum being stripped.
- Done in Fayetteville
- Staphy (not bad strain)
- Lipitor, crestor, beta blocker TBARS = thiobarbituric acid reactive substances
- Ultra performance liquid chromatography
- I thought you might want to use this slide. If not I will
- This slide would show how the pine could be put into practice.
- Picture of our study for environmental traits
- Here is a slide that either of us could use on how we harvested the tissue for the current study.
- This slide shows how we could potentially extract from live trees.
- Look at month trees
- Here Ill talk about how the target terpenes are correlated and then Ill go on to talk about the results with environmental and monthly trends.
- Shameless plugs