PCR and electrophoresis can be used to identify DNA samples taxonomically. DNA is extracted, amplified through PCR, and run on a gel for electrophoresis. Band patterns are interpreted to identify species. DNA hybridization compares similarity, but has low sensitivity, so RFLP is used instead to detect interspecies variation through restriction enzyme digestion and separation by size on a gel.

1. Polymearase Chain Reaction and Electrophoresis can used employed in taxonomic identification
of DNA samples.
The test DNA is extracted -> DNA is amplified -> PCR -> Gel Electrophoresis -> Interpretation
of results -> Species identification.
The test DNA can be compared with another DNA sample to compare similarity by DNA
hybridization technique. If both the single stranded DNA hybridize and form a double helix, it
indicates, they are closely related and if hybridization does not happen, it indicates they are
distantly related. The amount of hybridization can be measured by reading the absorbance.
Single stranded DNA absorbs more UV radiation than double stranded DNA. Hybridization is of
low sensitivity. Hence RFLP is employed to compare DNA for taxonomic identification.
The RFLP analysis is widely used for the detection of interspecies variation at the DNA
sequence level. It consists in the generation of species-specific band profiles through the
digestion of DNA with one or more restriction endonucleases. These restriction enzymes cleave
the DNA molecule at specific 4-6 base pair (bp) recognition sites, originating a set of fragments
with different lengths that could be separated according to their molecular size by conventional
gel electrophoresis. The RFLP banding pattern could be visualized by hybridizing restriction
fragments with a labelled probe in a solid support (for instance, by Southern blotting) or by
treating the electrophoretic gel with ethidium bromide or silver staining.
Solution
Polymearase Chain Reaction and Electrophoresis can used employed in taxonomic identification
of DNA samples.
The test DNA is extracted -> DNA is amplified -> PCR -> Gel Electrophoresis -> Interpretation
of results -> Species identification.
The test DNA can be compared with another DNA sample to compare similarity by DNA
hybridization technique. If both the single stranded DNA hybridize and form a double helix, it
indicates, they are closely related and if hybridization does not happen, it indicates they are
distantly related. The amount of hybridization can be measured by reading the absorbance.
Single stranded DNA absorbs more UV radiation than double stranded DNA. Hybridization is of
low sensitivity. Hence RFLP is employed to compare DNA for taxonomic identification.
The RFLP analysis is widely used for the detection of interspecies variation at the DNA
sequence level. It consists in the generation of species-specific band profiles through the
digestion of DNA with one or more restriction endonucleases. These restriction enzymes cleave
the DNA molecule at specific 4-6 base pair (bp) recognition sites, originating a set of fragments

2. with different lengths that could be separated according to their molecular size by conventional
gel electrophoresis. The RFLP banding pattern could be visualized by hybridizing restriction
fragments with a labelled probe in a solid support (for instance, by Southern blotting) or by
treating the electrophoretic gel with ethidium bromide or silver staining.