The document discusses protein isolation and polyacrylamide gel electrophoresis (PAGE). Plasminogen activator, an enzyme that prevents blood clotting, is being isolated from Hela cells using magnetic nanoparticles. Chromatography and zymography help determine the presence and activity of proteins. PAGE was then used to separate the isolated proteins by size. During the experiment, it was found that one of the reactants was contaminated based on abnormal absorbency readings.