This document evaluates the plant pathogen biocontrol and growth enhancement abilities of Pseudomonas koreinsis QFR5 in a shisham (Dalbergia sissoo) nursery. Key findings include: 1. Strain QFR5 showed strong antagonism against 4 pathogenic fungi (Fusarium oxysporum, Aspergillus calidoustus, Talaromyces pinophilus, Talaromyces verruculosus) isolated from diseased shisham in vitro. 2. Inoculation with strain QFR5 led to increased nodulation, plant biomass, soil enzyme activity, and decreased disease incidence in shisham cultivars PS90

1. Evaluation of Pseudomonas koreinsis QFR5 for
plant pathogen biocontrol and growth enhancement
in shisham nursery
HEMANT DASILA, SAMIKSHA JOSHI, ANAMIKA, DAMINI MAITHANI
AND MANVIKA SAHGAL1
DEPARTMENT OF MICROBIOLOGY, CBSH

2. INTRODUCTION
Pseudomonads control deleterious microorganisms.
Various mechanism of control.

3. OBJECTIVES
I. Isolation of phytopathogenic fungi from rhizosphere of diseased Dalbergia
sissoo and pathogenicity trial on homologous host.
II. In Vitro Antagonism of Pseudomonas strain QFR5 against isolated
phytopathogenic fungi.
III. Evaluating Plant Growth Promoting Efficacy Of Pseudomonas strain QFR5 on
growth and establishment of Dalbergia sissoo nursery.

4. PATHOGENICITY TRIAL
Table 1: Disease incidence percentage(%) during pathogenicity trial.
S.NO CODE Name(18S region) Disease Incidence
(%)
1. R1
Fusarium
oxysporum 75
2. R2
Aspergillus
calidoustus 75
3. R3
Talaromyces
pinophilus 50
4. R4
Talaromyces
verruculosus 50

5. R1 R2 R3 R4
In- vitro antagonism assay
R1 R2 R3 R4
QFR5 Whole Cell and
Supernatant

6. Table 2: Nodulation pattern of D. sissoo cultivar PS 90 and PS 52 as influenced
by inoculation of Pseudomonas koreinsis QFR5 during pot trial.
NODULULATION NODULE NUMBER
D.sissoo
cultivar
Treatment
SAMPLING SCHELDUDE (DAYS) SAMPLING SCHELDUDE (DAYS)
30 60 90 30 60 90
PS 90
T1(UNINOCU
LATED)
-ve +ve +ve 0 4 15
T2(CMC+
QFR5)
+ve +ve +ve 7 11 18
T3(GA+
QFR5)
-ve +ve +ve 0 6 16
PS 52
T4(UNINOCU
LATED)
+ve +ve +ve 5 12 24
T5(CMC+
QFR5)
+ve +ve +ve 12 8 24
T6(GA+
QFR5)
-ve +ve +ve 0 7 25

7. Table 3: Plant biomass of Dalbergia sissoo cultivar PS 90 influenced by
inoculation of Pseudomonas koreinsis QFR5 following two different
coating methods in pot culture experiments under glass house
conditions in nursery.
PLANT BIOMASS
TREATMENT
30 DAI 60 DAI 90 DAI
SFW
(gm)
SL
(cm)
RFW
(gm)
RL
(cm)
SFW
(gm)
SL
(cm)
RFW
(gm)
RL
(cm)
SFW
(gm)
SL(cm)
RFW
(gm)
RL
(cm)
T1(UNINO
CULATED)
1.1A 25A 0.56A 9A 2.3A 33A 1.3A 17.1A 5.8AB 44.4A 2.8B 17.6A
T2(CMC+Q
FR5)
1.2AB 30B 0.8A 18C 3.2B 35.4B 2.1B 21B 4.6AB 36.2B 1.4A 21.5B
T3(GA+QF
R5)
1.8B 40C 0.9B 10B 3.3B 44.6C 1.4A 22.4B 5.8AB 45.6C 1.6A 25C
CD AT 5% 0.22 1.3 0.24 0.9 0.44 1.45 0.6 1.54 0.77 1.45 0.6 1.54

8. Table 4: Plant biomass of Dalbergia sissoo cultivar PS 52 influenced by
inoculation of Pseudomonas koreinsis QFR5 following two different
coating methods in pot culture experiments under glass house
conditions in nursery.
TREATMENT
30 DAI 60 DAI 90 DAI
SFW
(gm)
SL(cm)
RFW
(gm)
RL(cm)
SFW
(gm)
SL(cm)
RFW
(gm)
RL(cm)
SFW
(gm)
SL(cm)
RFW
(gm)
RL
(cm)
T4(UNINO
CULATED)
1.6AB 30A 0.85C 16B 2.6B 34A 1.4B 21A 2.5A 36A 1.5AB 21.5A
T5(CMC+
QFR5)
1.8B 32B 0.11A 15B 2.4B 35.8B 1.2A 23B 2.6B 36.4A 1.4A 24B
T6(GA+
QFR5)
1.0A 35C 0.6B 10A 1.9A 39C 1.2A 24B 2.5A 40B 1.7B 24.8C
CD AT5% 0.6 1.2 0.06 1.3 0.3 1.21 0.01 1.1 0.06 1.3 0.2 0.3

9. SOIL MICROBIAL ACTIVITY
Graph A and B showing Alkaline Phosphatase Activity of PS90 and PS52.
0
5
10
15
20
25
30
35
40
45
30 60 90
µg/mlhr
days after inoculation
T1
T2
T3
PS90
0
10
20
30
40
50
30 60 90
µg/mlhr
days after inoculation
T4
T5
T6
PS52

10. Graph C AND D showing FDA Hydrolysis Activity of PS90 and PS52.
0
5
10
15
20
25
30
30 60 90
µg/mlhr
days after inoculation
T4
T5
T6
PS52
0
5
10
15
20
25
30
30 60 90
µg/mlhr
days after inoculation
T1
T2
T3
PS90

11. PHYSICOCHEMICAL PROPERTIES OF SOIL.
CULTIVAR TREATMENT
EC
(dsm-1)
Nitrogen
(kg ha-1)
Phosphorous
(kg ha-1)
Potassium
(kg ha-1)
Organic
Carbon(%)
pH
PS 90
T1(UNINOCULATED) 0.30 270 23.08 235.0 1.14 7.1
T2(CMC+QFR5) 0.31 258.13 18.47 200.0 1.02 7.28
T3(GA+QFR5) 0.35 245.86 21.03 206.0 0.98 7.4
PS 52
T4(UNINOCULATED) 0.32 265.0 22.06 212.57 1.15 7.2
T5(CMC+QFR5) 0.34 260.92 19.85 199.55 1.08 7.16
T6(GA+QFR5) 0.35 270.24 18.98 200.89 1.0 7.24
Table 6: At 30 DAI

12. Table 7: Disease incidence percentage of D. sissoo cultivar (PS90 and PS52) during pot trial
experiment.
D. sissoo
cultivar
Treatment DI (%)
D. sissoo
cultivar
Treatment DI (%)
PS90
T1 75
PS52
T4 30
T2 60 T5 35
T3 55 T6 30

13. CONCLUSION
• Identified strain QFR5 is Pseudomonas koriensis.
• Inoculation of QRF5 strain displayed great antagonistic potential against four phyto
pathogens and enhanced plant biomass of D.sissoo cultivar.
• Disease incidence was found less in case of PS90 cultivar as compared to PS52 when
inoculated with QFR-5 strain.