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Below is relevant cloning information for pET28 vectors. Identify the following parts by using
indicated numbers is red: -Where does the Lac repressor bind? - Where does T7 RNA
polymerase bind? -Where does transcription stop? -Where does translation start? - To clone a
gene of interest (GOI) in-frame (i.e. fusion protein) that contains the His/thrombine/T7 tags
identify a restriction site region.

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Below is relevant cloning information for pET28 vectors. Identify the.pdf

  • 1. Below is relevant cloning information for pET28 vectors. Identify the following parts by using indicated numbers is red: -Where does the Lac repressor bind? - Where does T7 RNA polymerase bind? -Where does transcription stop? -Where does translation start? - To clone a gene of interest (GOI) in-frame (i.e. fusion protein) that contains the His/thrombine/T7 tags identify a restriction site region.