what kind of short tandem repeats DNA markers can be used for tracing paternal lineage in DNA ancestry testing? Solution DNA Ancestry testing can help you to trace your paternal and maternal lineages. There are two types of DNA Ancestry tests available: Y-DNA STR marker testing for tracing paternal lineage and mtDNA sequencing for tracing maternal lineage. Y-DNA test method: PCR analysis of short tandem repeat (STR) markers in the Y-DNA. 20 to 101 Y-DNA STR markers can be tested..