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Match the following incubation periods with their function. First incubation: incubation of the DNA
at 37C Choose.. Second incubation: incubation of the DNA and competent cells on ice Allows the
cells to express the new DNA Allows the DNAse (if present in that treatment) to degrade the pDNA
Third incubation: brief incubation of the competent cells at 42C Heat shock--this encourages the
competent cells to start healing themselves Allows the DNA to enter the cells Fourth incubation:
cells are put on ice Cells start to repair their membranes Fifth incubation: cells are incubated in
liquid medium at 37C Choose...

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  • 1. Match the following incubation periods with their function. First incubation: incubation of the DNA at 37C Choose.. Second incubation: incubation of the DNA and competent cells on ice Allows the cells to express the new DNA Allows the DNAse (if present in that treatment) to degrade the pDNA Third incubation: brief incubation of the competent cells at 42C Heat shock--this encourages the competent cells to start healing themselves Allows the DNA to enter the cells Fourth incubation: cells are put on ice Cells start to repair their membranes Fifth incubation: cells are incubated in liquid medium at 37C Choose...