One case of paternity testing has been given in the figure. Four autoradiographs (autorads) that
show DNA \"fingerprints\" for three individuals: a mother, her child, and the child\'s alleged
father(said father/without proof). Each autorad compares the traits inherited by these three
individuals - four traits are looked at, one for each autorad.
Presence or absence of different alleles (called DNA fingerprints) has been compared in each
case. These alleles (DNA fragments) differ in length from person to person; for this reason they
are used as genetic markers.
In case I-these alleles are A, B, C and D
In case II-E, G and F
In case III-H, I, J and K
In case IV-L, M, N and O
Ans 1
Case
Genotype of Mother
Genotype of Child
Genotype of alleged father
I
A/D
A/C
B/C
II
G/G
E/G
E/F
III
I/J
I/K
H/K
IV
M/N
M/O
L/O
Ans 2
In each case, child has inherited one copy of allele from mother and one from father so by
looking at the genotype of child, contribution from the parents can be predicted (by comparing
the genotype of all three persons)
Case
Genotype of child
Allele inherited from mother
Allele inherited from father
I
A/C
A
C
II
E/G
G
E
III
I/K
I
K
IV
M/O
M
O
Ans-3
In each case, we can see that child has inherited one copy of allele from mother and one from
father. So by making the comparison, it can be predicted that that the alleged father could be the
biological father of this child.
Ans 4
The DNA fingerprint assay is also useful in chimerism analysis after liver transplantation. Four
percent of donor lymphocytes (transient lymphocytes) are present for up to 3 weeks after
transplantation. If the donor lymphocytes persist, they can cause graft-versus-host disease
(GVHD), which is an underdiagnosed and often fatal complication that occurs in approximately
1% of cases, usually 2 to 6 weeks after transplantation. The following cases illustrate the utility
of the this assay in chimerism analysis.
A liver transplant recipient presented with a skin rash 2 weeks after transplant, and a punch
biopsy of the skin was obtained. The pathologist noted the presence of a lymphocyte infiltrate in
the dermis. Possible causes included a drug reaction, a viral infection, or early GVHD. DNA was
extracted from the area of lymphocyte infiltration, and DNA fingerprinting analysis was
performed. The results showed that some of the lymphocytes were of donor origin, confirming
the diagnosis of early GVHD.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1200713/
Case
Genotype of Mother
Genotype of Child
Genotype of alleged father
I
A/D
A/C
B/C
II
G/G
E/G
E/F
III
I/J
I/K
H/K
IV
M/N
M/O
L/O
Solution
One case of paternity testing has been given in the figure. Four autoradiographs (autorads) that
show DNA \"fingerprints\" for three individuals: a mother, her child, and the child\'s alleged
father(said father/without proof). Each autorad compares the traits inherited by these three
individuals - four traits are looked at, one for each autorad.
Presence or absence of different alleles.
One case of paternity testing has been given in the figure. Four aut.pdf
1. One case of paternity testing has been given in the figure. Four autoradiographs (autorads) that
show DNA "fingerprints" for three individuals: a mother, her child, and the child's alleged
father(said father/without proof). Each autorad compares the traits inherited by these three
individuals - four traits are looked at, one for each autorad.
Presence or absence of different alleles (called DNA fingerprints) has been compared in each
case. These alleles (DNA fragments) differ in length from person to person; for this reason they
are used as genetic markers.
In case I-these alleles are A, B, C and D
In case II-E, G and F
In case III-H, I, J and K
In case IV-L, M, N and O
Ans 1
Case
Genotype of Mother
Genotype of Child
Genotype of alleged father
I
A/D
A/C
B/C
II
G/G
E/G
E/F
III
I/J
I/K
H/K
IV
M/N
M/O
L/O
Ans 2
In each case, child has inherited one copy of allele from mother and one from father so by
looking at the genotype of child, contribution from the parents can be predicted (by comparing
2. the genotype of all three persons)
Case
Genotype of child
Allele inherited from mother
Allele inherited from father
I
A/C
A
C
II
E/G
G
E
III
I/K
I
K
IV
M/O
M
O
Ans-3
In each case, we can see that child has inherited one copy of allele from mother and one from
father. So by making the comparison, it can be predicted that that the alleged father could be the
biological father of this child.
Ans 4
The DNA fingerprint assay is also useful in chimerism analysis after liver transplantation. Four
percent of donor lymphocytes (transient lymphocytes) are present for up to 3 weeks after
transplantation. If the donor lymphocytes persist, they can cause graft-versus-host disease
(GVHD), which is an underdiagnosed and often fatal complication that occurs in approximately
1% of cases, usually 2 to 6 weeks after transplantation. The following cases illustrate the utility
of the this assay in chimerism analysis.
A liver transplant recipient presented with a skin rash 2 weeks after transplant, and a punch
biopsy of the skin was obtained. The pathologist noted the presence of a lymphocyte infiltrate in
the dermis. Possible causes included a drug reaction, a viral infection, or early GVHD. DNA was
extracted from the area of lymphocyte infiltration, and DNA fingerprinting analysis was
3. performed. The results showed that some of the lymphocytes were of donor origin, confirming
the diagnosis of early GVHD.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1200713/
Case
Genotype of Mother
Genotype of Child
Genotype of alleged father
I
A/D
A/C
B/C
II
G/G
E/G
E/F
III
I/J
I/K
H/K
IV
M/N
M/O
L/O
Solution
One case of paternity testing has been given in the figure. Four autoradiographs (autorads) that
show DNA "fingerprints" for three individuals: a mother, her child, and the child's alleged
father(said father/without proof). Each autorad compares the traits inherited by these three
individuals - four traits are looked at, one for each autorad.
Presence or absence of different alleles (called DNA fingerprints) has been compared in each
case. These alleles (DNA fragments) differ in length from person to person; for this reason they
are used as genetic markers.
In case I-these alleles are A, B, C and D
In case II-E, G and F
In case III-H, I, J and K
4. In case IV-L, M, N and O
Ans 1
Case
Genotype of Mother
Genotype of Child
Genotype of alleged father
I
A/D
A/C
B/C
II
G/G
E/G
E/F
III
I/J
I/K
H/K
IV
M/N
M/O
L/O
Ans 2
In each case, child has inherited one copy of allele from mother and one from father so by
looking at the genotype of child, contribution from the parents can be predicted (by comparing
the genotype of all three persons)
Case
Genotype of child
Allele inherited from mother
Allele inherited from father
I
A/C
A
C
II
E/G
5. G
E
III
I/K
I
K
IV
M/O
M
O
Ans-3
In each case, we can see that child has inherited one copy of allele from mother and one from
father. So by making the comparison, it can be predicted that that the alleged father could be the
biological father of this child.
Ans 4
The DNA fingerprint assay is also useful in chimerism analysis after liver transplantation. Four
percent of donor lymphocytes (transient lymphocytes) are present for up to 3 weeks after
transplantation. If the donor lymphocytes persist, they can cause graft-versus-host disease
(GVHD), which is an underdiagnosed and often fatal complication that occurs in approximately
1% of cases, usually 2 to 6 weeks after transplantation. The following cases illustrate the utility
of the this assay in chimerism analysis.
A liver transplant recipient presented with a skin rash 2 weeks after transplant, and a punch
biopsy of the skin was obtained. The pathologist noted the presence of a lymphocyte infiltrate in
the dermis. Possible causes included a drug reaction, a viral infection, or early GVHD. DNA was
extracted from the area of lymphocyte infiltration, and DNA fingerprinting analysis was
performed. The results showed that some of the lymphocytes were of donor origin, confirming
the diagnosis of early GVHD.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1200713/
Case
Genotype of Mother
Genotype of Child
Genotype of alleged father
I
A/D
A/C
B/C
