The document discusses aroma analysis techniques used by Mars Petcare's WALTHAM Centre for Pet Nutrition. It summarizes that aroma analysis involves sample preparation, compound identification using gas chromatography-mass spectrometry, and compound quantification. Key techniques described include headspace extraction, solvent extraction, gas chromatography separation of compounds, mass spectrometry identification against libraries, and gas chromatography-mass spectrometry-olfactometry to correlate aroma with specific compounds.

1. FLAVOUR CHEMISTRY
An Industry Perspective of
Flavour Analysis
Mars Petcare, WALTHAM Centre for Pet Nutrition
Lewis Jones
April 2017
1
This document and the information it contains are confidential and the property of Mars Incorporated. They may not in
any way be disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016

2. Overview
1. Introduction to Mars
2. Introduction to WALTHAM Centre for Pet Nutrition
3. Aroma Analysis
2
This document and the information it contains are confidential and the property of Mars Incorporated. They may not in
any way be disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016

3. Overview
1. Introduction to Mars
2. Introduction to WALTHAM Centre for Pet Nutrition
3. Aroma Analysis
3
This document and the information it contains are confidential and the property of Mars Incorporated. They may not in
any way be disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016

4. Mars
4
This document and the information it contains are confidential and the property of Mars Incorporated. They may not in
any way be disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016

5. Mars - Segments
Employ more than 72,000 Associates in 74 countries
5
Petcare
Wrigley
Chocolate
Drinks
Symbioscience
Food
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any way be disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016

6. Mars - Brands
6
Petcare
Wrigley
Chocolate
This document and the information it contains are confidential and the property of Mars Incorporated. They may not in
any way be disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016

7. Mars
• Our portfolio of brands includes 11 $1 billion dollar
brands
• Quality, Great Taste and Value!
7
This document and the information it contains are confidential and the property of Mars Incorporated. They may not in
any way be disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016

8. Mars – Fundamental Flavour Science Centres
8
Hackettstown, New Jersey
Chocolate
Chicago
Wrigley
Waltham-on-the-Wolds
Petcare
This document and the information it contains are confidential and the property of Mars Incorporated. They may not in
any way be disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016

9. Overview
1. Introduction to Mars
2. Introduction to WALTHAM Centre for Pet Nutrition
3. Aroma Analysis
9
This document and the information it contains are confidential and the property of Mars Incorporated. They may not in
any way be disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016

10. WALTHAM Centre for Pet Nutrition
This document and the information it contains are confidential and the property of Mars Incorporated. They may not in
any way be disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016
10

11. WALTHAM is the global fundamental science centre providing
scientific support, credibility and knowledge to Mars Petcare
WALTHAM® is the Fundamental Science
Behind our Brands
This document and the information it contains are confidential and the property of Mars Incorporated. They may not in
any way be disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016
11

12. High quality innovative science…
…conducted in a caring way
WALTHAM® Mission
12
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disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016

13. WALTHAM® Current Research
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disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016
13

14. Establishing
WALTHAM &
Mars Petcare
as the industry
leaders in
nutrition
Nutrition and metabolism
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disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016
14

15. Generating
fundamental
understanding of oral
disease: identifying
new ways to combat
it, helping vets
and consumers believe
in our products
Oral Care
This document and the information it contains are confidential and the property of Mars Incorporated. They may not in any way be
disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016
15

16. Understanding
how the dog
processes
food
Digestive Health
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disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016
16

17. Understanding
the impact of pets on
child development,
health and wellbeing of
the elderly and wider
community health
Human-Animal Interaction
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disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016
17

18. Understanding
how cats perceive
taste and aroma and
which ingredients
provide superior
feeding performance
Feeding Performance
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disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016
18

19. Understanding
how cats perceive
taste and aroma and
which ingredients
provide superior
feeding performance
Feeding Performance
What chemical
compounds give the
flavour to food
animals like
to eat
This document and the information it contains are confidential and the property of Mars Incorporated. They may not in any way be
disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016
19

20. Overview
1. Introduction to Mars
2. Introduction to The WALTHAM Centre for Pet Nutrition
3. Aroma Analysis
20
This document and the information it contains are confidential and the property of Mars Incorporated. They may not in
any way be disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016

21. Petfood Aroma Analysis
21
Sample Preparation
Compound Identification
Compound Quantification
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any way be disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016

22. Petfood Aroma Analysis
Sample Preparation Considerations
22
1. Reineccius, G., (2005) In: Flavor chemistry and technology, second edition; CRC Press: p. 33-72.
2. van Gemert, L.J., (2003) Compilations of flavour thresholds values in water and other media. Huizen:
Boelens Aroma Chemical Information Services. 385.
Volatility Sensitivity MatrixDiversity Stability
b.p.: 3 OC b.p.: 178 OC b.p.: 257 OC
Threshold: 600 μg/L Threshold: 5x10-3 μg/LThreshold: 0.01 μg/L Threshold: 23 mg/L
Threshold: 0.6 μg/L
kOW: 1.1 kOW: 74.1
heat
Threshold: 2x10-5 μg/L
There is no one perfect technique for aroma analysis!
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any way be disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016

23. 23
Solvent Solvent ExtractHeadspace Extraction
There is no one perfect technique for aroma analysis!
Petfood Aroma Analysis
Sample Preparation Considerations
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any way be disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016

24. 24
Solvent Assisted Flavour
Evaporation (SAFE).
Vacuum Distillation Evaporation
Petfood Aroma Analysis
Sample Preparation Considerations
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any way be disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016

25. 25
Sample Preparation
Compound Identification
Compound Quantification
Petfood Aroma Analysis
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any way be disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016

26. Petfood Aroma Analysis
Compound Identification
Gas Chromatography
26
GAS FLOW
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disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016

27. Gas Chromatography
27
GAS FLOW
Petfood Aroma Analysis
Compound Identification
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disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016

28. Gas Chromatography
28
Petfood Aroma Analysis
Compound Identification
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disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016

29. Gas Chromatography
29
Petfood Aroma Analysis
Compound Identification
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disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016

30. Gas Chromatography
30
Separation based on compounds boiling point
and interaction with liquid phase
Petfood Aroma Analysis
Compound Identification
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disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016

31. Gas Chromatography
31
Petfood Aroma Analysis
Compound Identification
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disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016

32. Gas Chromatography
32
Petfood Aroma Analysis
Compound Identification
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disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016

33. Gas Chromatography
33
Petfood Aroma Analysis
Compound Identification
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disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016

34. Gas Chromatography
34
Petfood Aroma Analysis
Compound Identification
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disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016

35. Gas Chromatography
35
Petfood Aroma Analysis
Compound Identification
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disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016

36. Gas Chromatography
36
Petfood Aroma Analysis
Compound Identification
This document and the information it contains are confidential and the property of Mars Incorporated. They may not in any way be
disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016

37. Gas Chromatography
37
Petfood Aroma Analysis
Compound Identification
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disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016

38. Gas Chromatograph
38
Time
Abundance
Petfood Aroma Analysis
Compound Identification
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disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016

39. • Identification of
compound’s by GC-
MS
• NIST 08 MS library
combined with Wiley
9th addition MS Library
has 667,000 unique
compounds
39
Petfood Aroma Analysis
Compound Identification
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any way be disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016

40. 40
2 .0 0 4 .0 0 6 .0 0 8 .0 0 1 0 .0 0 1 2 .0 0 1 4 .0 0 1 6 .0 0 1 8 .0 0 2 0 .0 0 2 2 .0 0 2 4 .0 0
5 0 0 0 0 0
1 0 0 0 0 0 0
1 5 0 0 0 0 0
2 0 0 0 0 0 0
2 5 0 0 0 0 0
3 0 0 0 0 0 0
3 5 0 0 0 0 0
4 0 0 0 0 0 0
m e -->
u n d a n c e
T IC : L iv e rFD 1 _ C O W e xtra c t_ 1 1 0 3 3 0 .D d a ta .m s
Time / minute
Intensity/units
Over 200 compounds!
Petfood Aroma Analysis
Compound Identification
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any way be disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016

41. 41
Petfood Aroma Analysis
Compound Identification
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any way be disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016

42. 42
Gas Chromatography – Mass Spectrometry – Olfactometry (GC-MS-O)
Petfood Aroma Analysis
Compound Identification
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any way be disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016

43. 43
Gas Chromatography – Mass Spectrometry – Olfactometry (GC-MS-O)
Petfood Aroma Analysis
Compound Identification
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any way be disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016

44. 44
Petfood Aroma Analysis
Compound Identification
Gas Chromatography – Mass Spectrometry – Olfactometry (GC-MS-O)
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any way be disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016

45. 45
Petfood Aroma Analysis
Compound Identification
Gas Chromatography – Mass Spectrometry – Olfactometry (GC-MS-O)
This document and the information it contains are confidential and the property of Mars Incorporated. They may not in
any way be disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016

46. 46
Petfood Aroma Analysis
Compound Identification
Gas Chromatography – Mass Spectrometry – Olfactometry (GC-MS-O)
This document and the information it contains are confidential and the property of Mars Incorporated. They may not in
any way be disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016

47. 47
Petfood Aroma Analysis
Compound Identification
Gas Chromatography – Mass Spectrometry – Olfactometry (GC-MS-O)
This document and the information it contains are confidential and the property of Mars Incorporated. They may not in
any way be disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016

48. 48
Petfood Aroma Analysis
Compound Identification
Gas Chromatography – Mass Spectrometry – Olfactometry (GC-MS-O)
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any way be disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016

49. 49
Petfood Aroma Analysis
Compound Identification
Gas Chromatography – Mass Spectrometry – Olfactometry (GC-MS-O)
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any way be disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016

50. 50
Petfood Aroma Analysis
Compound Identification
Gas Chromatography – Mass Spectrometry – Olfactometry (GC-MS-O)
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any way be disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016

51. 51
Petfood Aroma Analysis
Compound Identification
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any way be disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016

52. This document and the information it contains are confidential and the property of Mars Incorporated. They may not in
any way be disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016
52
Aroma Extract Dilution Analysis
Identify aroma peaks.
1/2
1/4
1/8
Screen importance of peaks
Petfood Aroma Analysis
Compound Identification

53. No. Odour Quality Retension Index Dilution Number
Flavour Dilution
Factor
1Malty 903 1 2
2Sulfur 993 5 32
3Green/butter 1067 0 1
4Cracker 1320 1 2
5Onion 1365 2 4
6Vinegar 1412 3 8
7Coffee 1414 3 8
8Potato 1438 8 256
9Cheese 1594 4 16
10Cracker 1630 1 2
11Cheese 1639 3 8
12Garlic/fatty 1699 0 1
13Popcorn 1741 7 128
14Beef/nutty 1782 3 8
15Sweet 1832 0 1
16Brown 1965 0 1
17Candyfloss 1998 13 8192
18Caramel 2105 1 2
19Curry 2160 3 8
20Brown 2243 2 4
21Floral 2257 0 1
22Floral/pungent 2365 1 2
23Heavy/floral 2423 1 2
24Floral/faecal 2464 4 16
25Soapy/floral 2473 2 4
26Flux 2600 0 1
0
2
4
6
8
10
12
14
DilutionNumber
Retention Index
53
Petfood Aroma Analysis
Compound Identification
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any way be disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016

54. 54
6 . 0 0 8 . 0 0 1 0 . 0 0 1 2 . 0 0 1 4 . 0 0 1 6 . 0 0 1 8 . 0 0 2 0 . 0 0 2 2 . 0 0 2 4 . 0 0 2 6 . 0 0 2 8 . 0 0 3 0 . 0 0 3 2 . 0 0
1 0 0 0 0 0
2 0 0 0 0 0
3 0 0 0 0 0
4 0 0 0 0 0
5 0 0 0 0 0
6 0 0 0 0 0
7 0 0 0 0 0
8 0 0 0 0 0
9 0 0 0 0 0
1 0 0 0 0 0 0
1 1 0 0 0 0 0
1 2 0 0 0 0 0
1 3 0 0 0 0 0
1 4 0 0 0 0 0
1 5 0 0 0 0 0
1 6 0 0 0 0 0
1 7 0 0 0 0 0
1 8 0 0 0 0 0
e - - >
u n d a n c e
T I C : 4 g Y e a s t F D 1 1 1 0 2 0 8 . D d a t a . m s
Time / minute
Intensity/units
Petfood Aroma Analysis
Compound Identification
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any way be disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016

55. 55
6 . 0 0 8 . 0 0 1 0 . 0 0 1 2 . 0 0 1 4 . 0 0 1 6 . 0 0 1 8 . 0 0 2 0 . 0 0 2 2 . 0 0 2 4 . 0 0 2 6 . 0 0 2 8 . 0 0 3 0 . 0 0 3 2 . 0 0
1 0 0 0 0 0
2 0 0 0 0 0
3 0 0 0 0 0
4 0 0 0 0 0
5 0 0 0 0 0
6 0 0 0 0 0
7 0 0 0 0 0
8 0 0 0 0 0
9 0 0 0 0 0
1 0 0 0 0 0 0
1 1 0 0 0 0 0
1 2 0 0 0 0 0
1 3 0 0 0 0 0
1 4 0 0 0 0 0
1 5 0 0 0 0 0
1 6 0 0 0 0 0
1 7 0 0 0 0 0
1 8 0 0 0 0 0
e - - >
u n d a n c e
T I C : 4 g Y e a s t F D 1 1 1 0 2 0 8 . D d a t a . m s
Time / minute
Intensity/units
Not possible to identify all odour peaks!
Due to co-elution or missing peaks
Petfood Aroma Analysis
Compound Identification
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any way be disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016

56. 56
6 . 0 0 8 . 0 0 1 0 . 0 0 1 2 . 0 0 1 4 . 0 0 1 6 . 0 0 1 8 . 0 0 2 0 . 0 0 2 2 . 0 0 2 4 . 0 0 2 6 . 0 0 2 8 . 0 0 3 0 . 0 0 3 2 . 0 0
1 0 0 0 0 0
2 0 0 0 0 0
3 0 0 0 0 0
4 0 0 0 0 0
5 0 0 0 0 0
6 0 0 0 0 0
7 0 0 0 0 0
8 0 0 0 0 0
9 0 0 0 0 0
1 0 0 0 0 0 0
1 1 0 0 0 0 0
1 2 0 0 0 0 0
1 3 0 0 0 0 0
1 4 0 0 0 0 0
1 5 0 0 0 0 0
1 6 0 0 0 0 0
1 7 0 0 0 0 0
1 8 0 0 0 0 0
e - - >
u n d a n c e
T I C : 4 g Y e a s t F D 1 1 1 0 2 0 8 . D d a t a . m s
Time / minute
Intensity/units
Co-elution odour peaks
1 2 . 4 0 1 2 . 4 5 1 2 . 5 0 1 2 . 5 5 1 2 . 6 0 1 2 . 6 5 1 2 . 7 0 1 2 . 7 5
2 0 0 0 0 0
4 0 0 0 0 0
6 0 0 0 0 0
8 0 0 0 0 0
1 0 0 0 0 0 0
1 2 0 0 0 0 0
1 4 0 0 0 0 0
1 6 0 0 0 0 0
1 8 0 0 0 0 0
2 0 0 0 0 0 0
2 2 0 0 0 0 0
2 4 0 0 0 0 0
2 6 0 0 0 0 0
2 8 0 0 0 0 0
3 0 0 0 0 0 0
3 2 0 0 0 0 0
T im e -- >
A b u n d a n c e
T I C : 4 g Y e a s t F D 1 1 1 0 2 0 8 . D d a t a . m s
Petfood Aroma Analysis
Compound Identification
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any way be disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016

57. 57
Time / minute
5 . 0 0 1 0 . 0 0 1 5 . 0 0 2 0 . 0 0 2 5 . 0 0 3 0 . 0 0 3 5 . 0 0 4 0 . 0 0 4 5 . 0 0
5 0 0 0 0 0
1 0 0 0 0 0 0
1 5 0 0 0 0 0
2 0 0 0 0 0 0
2 5 0 0 0 0 0
3 0 0 0 0 0 0
3 5 0 0 0 0 0
4 0 0 0 0 0 0
4 5 0 0 0 0 0
m e - - >
u n d a n c e
T I C : F D 1 1 0 0 1 2 6 . D d a t a . m s
Intensity/units
Missing peaks!
8 . 8 0 9 . 0 0 9 . 2 0 9 . 4 0 9 . 6 0 9 . 8 0 1 0 . 0 0 1 0 . 2 0 1 0 . 4 0 1 0 . 6 0 1 0 . 8 0 1 1 . 0 0 1 1 . 2 0
5 0 0 0 0
1 0 0 0 0 0
1 5 0 0 0 0
2 0 0 0 0 0
2 5 0 0 0 0
3 0 0 0 0 0
3 5 0 0 0 0
4 0 0 0 0 0
4 5 0 0 0 0
T im e -->
A b u n d a n c e
T I C : F D 1 1 0 0 1 2 6 . D d a t a . m s
Petfood Aroma Analysis
Compound Identification
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any way be disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016

58. Need to:
1. Improve peak capacity (separation power)
2. Improve sensitivity, by increasing amount of
analyte to detector
58
Petfood Aroma Analysis
Compound Identification
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any way be disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016

59. Multidimensional Gas Chromatography – Mass Spectrometry –
Olfactometry with Integrated Preparative Fraction Collection
(GC-MS-O with PFC)
59
Petfood Aroma Analysis
Compound Identification
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any way be disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016

60. 60
&
Petfood Aroma Analysis
Compound Identification
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any way be disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016

61. 61
Petfood Aroma Analysis
Compound Identification
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any way be disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016

62. 62
Heart-cutting co-eluting peak
Petfood Aroma Analysis
Compound Identification
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any way be disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016

63. 63
Petfood Aroma Analysis
Compound Identification
Heart-cutting co-eluting peak
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any way be disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016

64. 64
Petfood Aroma Analysis
Compound Identification
Heart-cutting co-eluting peak
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any way be disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016

65. This document and the information it contains are confidential and the property of Mars Incorporated. They may not in
any way be disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016
65
Petfood Aroma Analysis
Compound Identification

66. • High volume cryotrap
(0.3 cm3)
• Self-packed (200mg
shown)
• No cryogenic
material required!
• Stable working temp
• -20 °C to 400 °C
(with oven at 320 °C)
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any way be disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016
66
66
High volume cryotrap enables multiple heart
cuts
Petfood Aroma Analysis
Compound Identification

67. 67
Petfood Aroma Analysis
Compound Identification
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any way be disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016

68. 68
Petfood Aroma Analysis
Compound Identification
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any way be disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016

69. 69
Petfood Aroma Analysis
Compound Identification
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any way be disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016

70. 70
Petfood Aroma Analysis
Compound Identification
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71. 71
Petfood Aroma Analysis
Compound Identification
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any way be disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016

72. 72
Petfood Aroma Analysis
Compound Identification
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73. 73
Petfood Aroma Analysis
Compound Identification
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74. 74
Petfood Aroma Analysis
Compound Identification
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75. 75
Petfood Aroma Analysis
Compound Identification
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any way be disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016

76. 76
Petfood Aroma Analysis
Compound Identification
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any way be disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016

77. 77
Petfood Aroma Analysis
Compound Identification
This document and the information it contains are confidential and the property of Mars Incorporated. They may not in
any way be disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016

78. 78
9 . 0 0 9 . 5 0 1 0 . 0 0 1 0 . 5 0 1 1 . 0 0 1 1 . 5 0 1 2 . 0 0 1 2 . 5 0 1 3 . 0 0
5 0 0 0 0 0
1 0 0 0 0 0 0
1 5 0 0 0 0 0
2 0 0 0 0 0 0
2 5 0 0 0 0 0
3 0 0 0 0 0 0
3 5 0 0 0 0 0
4 0 0 0 0 0 0
4 5 0 0 0 0 0
5 0 0 0 0 0 0
5 5 0 0 0 0 0
6 0 0 0 0 0 0
6 5 0 0 0 0 0
7 0 0 0 0 0 0
T i m e - - >
A b u n d a n c e
T I C : C O E L U T E _ 3 . D d a t a . m s
Time / minute
Intensity/units
1D-GC-O-MS
Petfood Aroma Analysis
Compound Identification
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79. 79
9 . 0 0 9 . 5 0 1 0 . 0 0 1 0 . 5 0 1 1 . 0 0 1 1 . 5 0 1 2 . 0 0 1 2 . 5 0 1 3 . 0 0
5 0 0 0 0 0
1 0 0 0 0 0 0
1 5 0 0 0 0 0
2 0 0 0 0 0 0
2 5 0 0 0 0 0
3 0 0 0 0 0 0
3 5 0 0 0 0 0
4 0 0 0 0 0 0
4 5 0 0 0 0 0
5 0 0 0 0 0 0
5 5 0 0 0 0 0
6 0 0 0 0 0 0
6 5 0 0 0 0 0
7 0 0 0 0 0 0
T i m e - - >
A b u n d a n c e
T I C : C O E L U T E _ 3 . D d a t a . m s
Intensity/units
Coffee
Vinegar
Jacket potato
Mushroom
Potato
1D-GC-O-MS
Petfood Aroma Analysis
Compound Identification
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80. 80
9 . 0 0 9 . 5 0 1 0 . 0 0 1 0 . 5 0 1 1 . 0 0 1 1 . 5 0 1 2 . 0 0 1 2 . 5 0 1 3 . 0 0 1 3 . 5 0
2 0 0 0 0
4 0 0 0 0
6 0 0 0 0
8 0 0 0 0
1 0 0 0 0 0
1 2 0 0 0 0
1 4 0 0 0 0
1 6 0 0 0 0
1 8 0 0 0 0
2 0 0 0 0 0
2 2 0 0 0 0
2 4 0 0 0 0
2 6 0 0 0 0
2 8 0 0 0 0
3 0 0 0 0 0
T i m e - - >
A b u n d a n c e
T I C : 1 5 1 0 1 3 C o e l u t e C u t v 1 r 0 1 . D d a t a . m s
T I C : 1 5 1 0 1 3 C o e l u t e C u t v 1 r 0 2 . D d a t a . m s ( * )
T I C : 1 5 1 0 1 3 C o e l u t e C u t v 1 r 0 3 . D d a t a . m s ( * )
Time / minute
Intensity/units
Petfood Aroma Analysis
Compound Identification
Heart-cutting co-eluting peak x3
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81. 81
Time / minute
Intensity/units
2 . 0 0 3 . 0 0 4 . 0 0 5 . 0 0 6 . 0 0 7 . 0 0 8 . 0 0 9 . 0 0 1 0 . 0 0
5 0 0 0 0 0
1 0 0 0 0 0 0
1 5 0 0 0 0 0
2 0 0 0 0 0 0
2 5 0 0 0 0 0
3 0 0 0 0 0 0
3 5 0 0 0 0 0
4 0 0 0 0 0 0
4 5 0 0 0 0 0
5 0 0 0 0 0 0
5 5 0 0 0 0 0
6 0 0 0 0 0 0
6 5 0 0 0 0 0
7 0 0 0 0 0 0
T i m e - - >
A b u n d a n c e
T I C : S E C O N D _ C U T _ E L U T E . D d a t a . m s
Petfood Aroma Analysis
Compound Identification
2D-GC-O-MS
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82. 82
Time / minute
Intensity/units
2 . 0 0 3 . 0 0 4 . 0 0 5 . 0 0 6 . 0 0 7 . 0 0 8 . 0 0 9 . 0 0 1 0 . 0 0
5 0 0 0 0 0
1 0 0 0 0 0 0
1 5 0 0 0 0 0
2 0 0 0 0 0 0
2 5 0 0 0 0 0
3 0 0 0 0 0 0
3 5 0 0 0 0 0
4 0 0 0 0 0 0
4 5 0 0 0 0 0
5 0 0 0 0 0 0
5 5 0 0 0 0 0
6 0 0 0 0 0 0
6 5 0 0 0 0 0
7 0 0 0 0 0 0
T i m e - - >
A b u n d a n c e
T I C : S E C O N D _ C U T _ E L U T E . D d a t a . m s
5 0 6 0 7 0 8 0 9 0 1 0 0 1 1 0 1 2 0 1 3 0 1 4 0 1
0
1 0 0 0 0 0
2 0 0 0 0 0
3 0 0 0 0 0
4 0 0 0 0 0
5 0 0 0 0 0
6 0 0 0 0 0
7 0 0 0 0 0
8 0 0 0 0 0
9 0 0 0 0 0
1 0 0 0 0 0 0
1 1 0 0 0 0 0
1 2 0 0 0 0 0
1 3 0 0 0 0 0
1 4 0 0 0 0 0
1 5 0 0 0 0 0
1 6 0 0 0 0 0
1 7 0 0 0 0 0
1 8 0 0 0 0 0
1 9 0 0 0 0 0
m / z - - >
A b u n d a n c e
S c a n 8 7 9 ( 5 . 3 4 4 m i n ) : S E C O N D _ C U T _
6 0 . 0
7 3 . 0 8 9 . 0 1 0 2 . 8 1 1 5 . 0 1 2 7 . 9
Vinegar - acetic acid
Petfood Aroma Analysis
Compound Identification
2D-GC-O-MS
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83. 83
Time / minute
Intensity/units
2 . 0 0 3 . 0 0 4 . 0 0 5 . 0 0 6 . 0 0 7 . 0 0 8 . 0 0 9 . 0 0 1 0 . 0 0
5 0 0 0 0 0
1 0 0 0 0 0 0
1 5 0 0 0 0 0
2 0 0 0 0 0 0
2 5 0 0 0 0 0
3 0 0 0 0 0 0
3 5 0 0 0 0 0
4 0 0 0 0 0 0
4 5 0 0 0 0 0
5 0 0 0 0 0 0
5 5 0 0 0 0 0
6 0 0 0 0 0 0
6 5 0 0 0 0 0
7 0 0 0 0 0 0
T i m e - - >
A b u n d a n c e
T I C : S E C O N D _ C U T _ E L U T E . D d a t a . m s
Coffee - furanmethanethiol
6 0 8 0 1 0 0 1 2 0 1 4 0 1 6 0 1 8 0 2
0
2 0 0 0 0
4 0 0 0 0
6 0 0 0 0
8 0 0 0 0
1 0 0 0 0 0
1 2 0 0 0 0
1 4 0 0 0 0
1 6 0 0 0 0
1 8 0 0 0 0
2 0 0 0 0 0
m / z -->
A b u n d a n c e
S c a n 1 3 4 6 (8 . 0 1 7 m in ): S E C O N D _ C U T _
8 1 . 0
6 0 . 0
1 1 4 . 0
1 3 2 . 8 1 7 6 . 9
Petfood Aroma Analysis
Compound Identification
2D-GC-O-MS
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84. 84
Time / minute
Intensity/units
2 . 0 0 3 . 0 0 4 . 0 0 5 . 0 0 6 . 0 0 7 . 0 0 8 . 0 0 9 . 0 0 1 0 . 0 0
5 0 0 0 0 0
1 0 0 0 0 0 0
1 5 0 0 0 0 0
2 0 0 0 0 0 0
2 5 0 0 0 0 0
3 0 0 0 0 0 0
3 5 0 0 0 0 0
4 0 0 0 0 0 0
4 5 0 0 0 0 0
5 0 0 0 0 0 0
5 5 0 0 0 0 0
6 0 0 0 0 0 0
6 5 0 0 0 0 0
7 0 0 0 0 0 0
T i m e - - >
A b u n d a n c e
T I C : S E C O N D _ C U T _ E L U T E . D d a t a . m s
Mushroom - 1-octen-3-ol
6 0 8 0 1 0 0 1 2 0 1 4 0 1 6 0 1 8 0 2 0 0 2 2 0 2 4 0
0
5 0 0 0 0
1 0 0 0 0 0
1 5 0 0 0 0
2 0 0 0 0 0
2 5 0 0 0 0
3 0 0 0 0 0
3 5 0 0 0 0
4 0 0 0 0 0
m / z -->
A b u n d a n c e
S c a n 1 4 2 9 (8 .4 9 2 m in ): S E C O N D _ C U T _ E L U T
5 7 .0
8 5 .0
1 1 0 .1 1 9 2 .91 3 5 .0
Petfood Aroma Analysis
Compound Identification
2D-GC-O-MS
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85. 85
Time / minute
Intensity/units
2 . 0 0 3 . 0 0 4 . 0 0 5 . 0 0 6 . 0 0 7 . 0 0 8 . 0 0 9 . 0 0 1 0 . 0 0
5 0 0 0 0 0
1 0 0 0 0 0 0
1 5 0 0 0 0 0
2 0 0 0 0 0 0
2 5 0 0 0 0 0
3 0 0 0 0 0 0
3 5 0 0 0 0 0
4 0 0 0 0 0 0
4 5 0 0 0 0 0
5 0 0 0 0 0 0
5 5 0 0 0 0 0
6 0 0 0 0 0 0
6 5 0 0 0 0 0
7 0 0 0 0 0 0
T i m e - - >
A b u n d a n c e
T I C : S E C O N D _ C U T _ E L U T E . D d a t a . m s
7 . 4 0 7 . 6 0 7 . 8 0 8 . 0 0 8 . 2 0 8 . 4 0 8 . 6 0 8 . 8 0 9 . 0 0 9 . 2 0
5 0 0 0 0
1 0 0 0 0 0
1 5 0 0 0 0
2 0 0 0 0 0
2 5 0 0 0 0
3 0 0 0 0 0
3 5 0 0 0 0
4 0 0 0 0 0
4 5 0 0 0 0
5 0 0 0 0 0
5 5 0 0 0 0
6 0 0 0 0 0
6 5 0 0 0 0
7 0 0 0 0 0
7 5 0 0 0 0
8 0 0 0 0 0
8 5 0 0 0 0
9 0 0 0 0 0
9 5 0 0 0 0
1 0 0 0 0 0 0
1 0 5 0 0 0 0
1 1 0 0 0 0 0
1 1 5 0 0 0 0
1 2 0 0 0 0 0
1 2 5 0 0 0 0
T i m e - - >
A b u n d a n c e
T I C : S E C O N D _ C U T _ E L U T E . D d a t a . m s
Potato Jacket Potato
Petfood Aroma Analysis
Compound Identification
2D-GC-O-MS
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86. 1 0 . 2 0 1 0 . 4 0 1 0 . 6 0 1 0 . 8 0 1 1 . 0 0 1 1 . 2 0 1 1 . 4 0 1 1 . 6 0 1 1 . 8 0 1 2 . 0 0 1 2 . 2 0 1 2 . 4 0
0
2 0 0 0 0
4 0 0 0 0
6 0 0 0 0
8 0 0 0 0
1 0 0 0 0 0
1 2 0 0 0 0
1 4 0 0 0 0
1 6 0 0 0 0
1 8 0 0 0 0
2 0 0 0 0 0
2 2 0 0 0 0
T im e - - >
A b u n d a n c e
T I C : 1 4 1 0 1 3 C o e lu t e C u t v 1 r 0 1 . D d a t a . m s
T I C : 1 4 1 0 1 3 C o e lu t e C u t v 1 r 0 2 . D d a t a . m s ( * )
T I C : 1 4 1 0 1 3 C o e lu t e C u t v 1 r 0 3 . D d a t a . m s ( * )
T I C : 1 4 1 0 1 3 C o e lu t e C u t v 1 r 0 4 . D d a t a . m s ( * )
T I C : 1 4 1 0 1 3 C o e lu t e C u t v 1 r 0 5 . D d a t a . m s ( * )
T I C : 1 4 1 0 1 3 C o e lu t e C u t v 1 r 0 6 . D d a t a . m s ( * )
T I C : 1 4 1 0 1 3 C o e lu t e C u t v 2 r 0 1 . D d a t a . m s ( * )
T I C : 1 4 1 0 1 3 C o e lu t e C u t v 2 r 0 2 . D d a t a . m s ( * )
T I C : 1 4 1 0 1 3 C o e lu t e C u t v 2 r 0 3 . D d a t a . m s ( * )
T I C : 1 4 1 0 1 3 C o e lu t e C u t v 2 r 0 4 . D d a t a . m s ( * )
T I C : 1 4 1 0 1 3 C o e lu t e C u t v 2 r 0 5 . D d a t a . m s ( * )
T I C : 1 4 1 0 1 3 C o e lu t e C u t v 2 r 0 6 . D d a t a . m s ( * )
T I C : 1 4 1 0 1 3 C o e lu t e C u t v 3 r 0 1 . D d a t a . m s ( * )
T I C : 1 4 1 0 1 3 C o e lu t e C u t v 3 r 0 2 . D d a t a . m s ( * )
T I C : 1 4 1 0 1 3 C o e lu t e C u t v 3 r 0 3 . D d a t a . m s ( * )
T I C : 1 4 1 0 1 3 C o e lu t e C u t v 3 r 0 4 . D d a t a . m s ( * )
T I C : 1 4 1 0 1 3 C o e lu t e C u t v 3 r 0 5 . D d a t a . m s ( * )
T I C : 1 4 1 0 1 3 C o e lu t e C u t v 3 r 0 6 . D d a t a . m s ( * )
T I C : 1 4 1 0 1 3 C o e lu t e C u t v 4 r 0 1 . D d a t a . m s ( * )
T I C : 1 4 1 0 1 3 C o e lu t e C u t v 4 r 0 2 . D d a t a . m s ( * )
T I C : 1 4 1 0 1 3 C o e lu t e C u t v 4 r 0 3 . D d a t a . m s ( * )
T I C : 1 4 1 0 1 3 C o e lu t e C u t v 4 r 0 4 . D d a t a . m s ( * )
T I C : 1 4 1 0 1 3 C o e lu t e C u t v 4 r 0 5 . D d a t a . m s ( * )
T I C : 1 4 1 0 1 3 C o e lu t e C u t v 4 r 0 6 . D d a t a . m s ( * )
86
Time / minute
Intensity/units
Petfood Aroma Analysis
Compound Identification
Heart-cutting co-eluting peak x 24
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87. 7 . 6 0 7 . 8 0 8 . 0 0 8 . 2 0 8 . 4 0 8 . 6 0 8 . 8 0 9 . 0 0 9 . 2 0 9 . 4 0 9 . 6 0 9 . 8 0
4 0 0 0 0 0
6 0 0 0 0 0
8 0 0 0 0 0
1 0 0 0 0 0 0
1 2 0 0 0 0 0
1 4 0 0 0 0 0
1 6 0 0 0 0 0
1 8 0 0 0 0 0
2 0 0 0 0 0 0
2 2 0 0 0 0 0
2 4 0 0 0 0 0
2 6 0 0 0 0 0
2 8 0 0 0 0 0
3 0 0 0 0 0 0
3 2 0 0 0 0 0
3 4 0 0 0 0 0
3 6 0 0 0 0 0
T i m e - - >
A b u n d a n c e
T I C : O V E R N I G H T _ C U T _ E L U T E . D d a t a . m s
87
Time / minute
Intensity/units
Petfood Aroma Analysis
Compound Identification
2D-GC-O-MS
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88. 7 . 6 0 7 . 8 0 8 . 0 0 8 . 2 0 8 . 4 0 8 . 6 0 8 . 8 0 9 . 0 0 9 . 2 0 9 . 4 0 9 . 6 0 9 . 8 0
4 0 0 0 0 0
6 0 0 0 0 0
8 0 0 0 0 0
1 0 0 0 0 0 0
1 2 0 0 0 0 0
1 4 0 0 0 0 0
1 6 0 0 0 0 0
1 8 0 0 0 0 0
2 0 0 0 0 0 0
2 2 0 0 0 0 0
2 4 0 0 0 0 0
2 6 0 0 0 0 0
2 8 0 0 0 0 0
3 0 0 0 0 0 0
3 2 0 0 0 0 0
3 4 0 0 0 0 0
3 6 0 0 0 0 0
T i m e - - >
A b u n d a n c e
T I C : O V E R N I G H T _ C U T _ E L U T E . D d a t a . m s
88
Time / minute
Intensity/units
Potato – 3-(methylthio)propanal
5 0 6 0 7 0 8 0 9 0 1 0 0 1 1 0 1 2 0 1 3 0
0
1 0 0 0 0
2 0 0 0 0
3 0 0 0 0
4 0 0 0 0
5 0 0 0 0
6 0 0 0 0
7 0 0 0 0
8 0 0 0 0
9 0 0 0 0
1 0 0 0 0 0
1 1 0 0 0 0
m / z - - >
A b u n d a n c e
S c a n 1 5 6 ( 7 . 9 7 9 m i n ) : O V E R N I G H T _ C
1 0 4 . 0
7 6 . 0
6 1 . 0
8 9 . 0
1 31 2 0 . 0
Petfood Aroma Analysis
Compound Identification
2D-GC-O-MS
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89. 7 . 6 0 7 . 8 0 8 . 0 0 8 . 2 0 8 . 4 0 8 . 6 0 8 . 8 0 9 . 0 0 9 . 2 0 9 . 4 0 9 . 6 0 9 . 8 0
4 0 0 0 0 0
6 0 0 0 0 0
8 0 0 0 0 0
1 0 0 0 0 0 0
1 2 0 0 0 0 0
1 4 0 0 0 0 0
1 6 0 0 0 0 0
1 8 0 0 0 0 0
2 0 0 0 0 0 0
2 2 0 0 0 0 0
2 4 0 0 0 0 0
2 6 0 0 0 0 0
2 8 0 0 0 0 0
3 0 0 0 0 0 0
3 2 0 0 0 0 0
3 4 0 0 0 0 0
3 6 0 0 0 0 0
T i m e - - >
A b u n d a n c e
T I C : O V E R N I G H T _ C U T _ E L U T E . D d a t a . m s
89
Time / minute
Intensity/units
Jacket potato - 2-ethyl-3,5(6)-dimethylpyrazine
6 0 8 0 1 0 0 1 2 0 1 4 0 1 6 0 1 8 0
0
5 0 0 0
1 0 0 0 0
1 5 0 0 0
2 0 0 0 0
2 5 0 0 0
3 0 0 0 0
3 5 0 0 0
4 0 0 0 0
4 5 0 0 0
m / z - - >
A b u n d a n c e
A v e r a g e o f 9 . 1 8 7 t o 9 . 1 9 8 m i n . : O V E R N I G H
1 3 5 . 0
1 0 8 . 0
5 6 . 0
8 0 . 0
1 7 7 . 91 5 4 . 8
Petfood Aroma Analysis
Compound Identification
2D-GC-O-MS
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90. 7 . 6 0 7 . 8 0 8 . 0 0 8 . 2 0 8 . 4 0 8 . 6 0 8 . 8 0 9 . 0 0 9 . 2 0 9 . 4 0 9 . 6 0 9 . 8 0
4 0 0 0 0 0
6 0 0 0 0 0
8 0 0 0 0 0
1 0 0 0 0 0 0
1 2 0 0 0 0 0
1 4 0 0 0 0 0
1 6 0 0 0 0 0
1 8 0 0 0 0 0
2 0 0 0 0 0 0
2 2 0 0 0 0 0
2 4 0 0 0 0 0
2 6 0 0 0 0 0
2 8 0 0 0 0 0
3 0 0 0 0 0 0
3 2 0 0 0 0 0
3 4 0 0 0 0 0
3 6 0 0 0 0 0
T i m e - - >
A b u n d a n c e
T I C : O V E R N I G H T _ C U T _ E L U T E . D d a t a . m s
90
Time / minute
Intensity/units
4 0 6 0 8 0 1 0 0 1 2 0 1 4 0 1 6 0 1 8 0 2 0 0 2 2 0 2
0
5 0 0 0
1 0 0 0 0
1 5 0 0 0
2 0 0 0 0
2 5 0 0 0
m / z -->
A b u n d a n c e
A v e ra g e o f 9 .2 1 5 to 9 .2 3 8 m in .: O V E R N IG H T
1 3 5 .1
5 6 .0
1 0 8 .0
8 0 .0 1 7 8 .9 2 0 8 .9
Petfood Aroma Analysis
Compound Identification
2D-GC-O-MS
Jacket potato - 2-ethyl-3,5(6)-dimethylpyrazine
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91. Petfood Aroma Analysis
91
Sample Preparation
Compound Identification
Compound Quantification
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any way be disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016

92. 92
Petfood Aroma Analysis
Compound Quantification
Quantification of
compounds by GC-FID
A – Analyte
IS – Internal Standard
A
IS
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93. 93
Petfood Aroma Analysis
Compound Quantification
Quantification of
compounds by GC-FID
A – Analyte
IS – Internal Standard
A
IS
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any way be disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016

94. Pf,a – Preparation factor
94
Massa =
Areaa
Areais
x Massis x Rf,a x Pf,a
Petfood Aroma Analysis
Compound Quantification
Quantification of
compounds by GC-FID
A – Analyte
IS – Internal Standard
A
IS
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95. 95
Internal Standard (IS)
Solvent Matrix Volatility & Solubility
Petfood Aroma Analysis
Compound Quantification
Massa =
Areaa
Areais
x Massis x Rf,a x Pf,a
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96. • Preparation factor (Pf,a) needs to be calculated for
every analyte of interest and for every matrix that
quantification is carried out in
• Solution is to use stable isotopes as internal
standards and quantify by GC-MS
96
Petfood Aroma Analysis
Compound Quantification
Massa =
Areaa
Areais
x Massis x Rf,a x Pf,a
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any way be disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016

97. • Using a stable isotope of the analyte as an internal standard can
assume the recovery of analyte and internal standard is the same in
every matrix.
• No preparation factor is needed!
97
3-methylbutanal
Molecular Weight - 86
[2H2]-3-methylbutanal
Molecular Weight - 88
Deuterium Labelling
Carbon 13 labelling
Norfuraneol
Molecular Weight - 114
[13C2]- Norfuraneol
Molecular Weight - 116
Petfood Aroma Analysis
Compound Quantification
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any way be disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016

98. 98
Stable Isotope as
Internal Standard
(IS)
Solvent Matrix Volatility & Solubility
Petfood Aroma Analysis
Compound Quantification
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any way be disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016

99. Rf,a – Response factor
A – Analyte
IS – Internal Standard
99
Areaa
Areais
= Rf,a
Massis
MassaA IS
Petfood Aroma Analysis
Compound Quantification
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any way be disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016

100. Rf,a – Response factor
A – Analyte
IS – Internal Standard
100
Areaa
Areais
= Rf,a
Dimethyltrisulfide Response
y = 1.8716x
R2
= 0.9962
0
1
2
3
4
5
6
0 0.5 1 1.5 2 2.5 3
Mass Ratio
PeakAreaRatio
Massis
MassaA IS
Petfood Aroma Analysis
Compound Quantification
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any way be disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016

101. Quantification of
compounds by GC-MS
101
Petfood Aroma Analysis
Compound Quantification
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any way be disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016

102. 102
1 8 .6 01 8 .8 01 9 .0 01 9 .2 01 9 .4 01 9 .6 01 9 .8 02 0 .0 02 0 .2 02 0 .4 02 0 .6 02 0 .8 02 1 .0 02 1 .2 02 1 .4 0
2 0 0 0 0 0 0
4 0 0 0 0 0 0
6 0 0 0 0 0 0
8 0 0 0 0 0 0
1 e + 0 7
1 .2 e + 0 7
1 .4 e + 0 7
1 .6 e + 0 7
T im e -->
A b u n d a n c e
T IC : G T F D 4 1 0 1 2 0 8 s 3 T IC .D d a ta .m s
1 9 .8 0 7
6 0 8 0 1 0 0 1 2 0 1 4 0 1 6 0 1 8 0 2 0 0 2 2 0 2 4 0 2 6 0 2 8 0
0
2 0 0 0 0 0
4 0 0 0 0 0
6 0 0 0 0 0
8 0 0 0 0 0
1 0 0 0 0 0 0
1 2 0 0 0 0 0
1 4 0 0 0 0 0
1 6 0 0 0 0 0
1 8 0 0 0 0 0
2 0 0 0 0 0 0
2 2 0 0 0 0 0
2 4 0 0 0 0 0
2 6 0 0 0 0 0
2 8 0 0 0 0 0
3 0 0 0 0 0 0
m / z - - >
A b u n d a n c e
S c a n 5 9 9 4 ( 1 9 . 8 0 0 m i n ) : G T F D 4 1 0 1 2 0 8 s 3 T I C . D d a t a . m s
1 2 8 . 1
5 7 . 1
8 5 . 0
1 1 1 . 1 1 7 1 . 11 4 5 . 1 1 9 1 . 1 2 1 9 . 1 2 7 8 . 12 4 7 . 2
1 8 .6 01 8 .8 01 9 .0 01 9 .2 01 9 .4 01 9 .6 01 9 .8 02 0 .0 02 0 .2 02 0 .4 02 0 .6 02 0 .8 02 1 .0 02 1 .2 02 1 .4 0
0
5 0 0 0 0 0
1 0 0 0 0 0 0
1 5 0 0 0 0 0
2 0 0 0 0 0 0
2 5 0 0 0 0 0
3 0 0 0 0 0 0
T ime -->
A b u n d a n c e
Io n 1 2 8 .0 0 (1 2 7 .7 0 to 1 2 8 .7 0 ): G T FD 4 1 0 1 2 0 8 s3 T IC.D d a ta .ms
1 9 .8 0 8
1 8 .6 01 8 .8 01 9 .0 01 9 .2 01 9 .4 01 9 .6 01 9 .8 02 0 .0 02 0 .2 02 0 .4 02 0 .6 02 0 .8 02 1 .0 02 1 .2 02 1 .4 0
0
5 0 0 0 0 0
1 0 0 0 0 0 0
1 5 0 0 0 0 0
2 0 0 0 0 0 0
2 5 0 0 0 0 0
3 0 0 0 0 0 0
T ime -->
A b u n d a n c e
Io n 1 3 0 .0 0 (1 2 9 .7 0 to 1 3 0 .7 0 ): G T FD 4 1 0 1 2 0 8 s3 T IC.D d a ta .ms
1 9 .8 0 6
1 8 .6 01 8 .8 01 9 .0 01 9 .2 01 9 .4 01 9 .6 01 9 .8 02 0 .0 02 0 .2 02 0 .4 02 0 .6 02 0 .8 02 1 .0 02 1 .2 02 1 .4 0
0
5 0 0 0 0 0
1 0 0 0 0 0 0
1 5 0 0 0 0 0
2 0 0 0 0 0 0
2 5 0 0 0 0 0
3 0 0 0 0 0 0
T ime -->
A b u n d a n c e
Io n 1 2 8 .0 0 (1 2 7 .7 0 to 1 2 8 .7 0 ): G T FD 4 1 0 1 2 0 8 s3 T IC.D d a ta .ms
1 9 .8 0 8
1 8 .6 01 8 .8 01 9 .0 01 9 .2 01 9 .4 01 9 .6 01 9 .8 02 0 .0 02 0 .2 02 0 .4 02 0 .6 02 0 .8 02 1 .0 02 1 .2 02 1 .4 0
0
5 0 0 0 0 0
1 0 0 0 0 0 0
1 5 0 0 0 0 0
2 0 0 0 0 0 0
2 5 0 0 0 0 0
3 0 0 0 0 0 0
T ime -->
A b u n d a n c e
Io n 1 3 0 .0 0 (1 2 9 .7 0 to 1 3 0 .7 0 ): G T FD 4 1 0 1 2 0 8 s3 T IC.D d a ta .ms
1 9 .8 0 6
Time / minute
Intensity/units
Mass / Daltons
Abundance/units
MS Fragmentation Pattern
Time / minute
Intensity/units
Selected 128 Ion Chromatogram
Time / minute
Intensity/units
Selected 130 Ion Chromatogram
Peak Area
85559454 units
Peak Area
82817293 units
Petfood Aroma Analysis
Compound Quantification
This document and the information it contains are confidential and the property of Mars Incorporated. They may not in
any way be disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016

103. Petfood Aroma Analysis - Overview
103
Sample Preparation
Compound Identification
Compound Quantification
This document and the information it contains are confidential and the property of Mars Incorporated. They may not in
any way be disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016

104. Thank You, Questions
This document and the information it contains are confidential and the property of Mars Incorporated. They may not in any way be
disclosed, copied or used by anyone except when expressly authorised by Mars Inc. ©Mars 2016
104