Sample Collection

1. BLOOD SAMPLE COLLECTION

2.  Threeveinin whichwe can collect
the bloodsample easily
1. Cephalic
2. Basilic
3. MedianCubital

4.  REQUIREMENTS:-
 Disposable gloves
 Alcohol swabs
 Tourniquet
 Vacationers
 Vacationerholder/sryinge
 Sterile disposableneedles
 Cottonballs
 Sharpsdisposal container
 BandAid

5.  All requiredmaterial shouldbe assembledbeforedrawthe sample.
 ChecktheLab requisitionformto confirmthe quantityof bloodto be drawn
andwhichkindof vacationertubesto use.

6. •Vacationersshouldbe labeledby Unique
IdentificationNumber(UIN),name and date of
sample collection.
•Thepatient/donorshouldsit in a chair or lie
downon a bed.
•Phlebotomistshouldwear disposablegloves
beforeblood sampling.
•Applytourniquetto exposeveins. It shouldnot
be placetoo tightly.

7.  Place patient’sarmin downwardposition.
 The exactsiteof veinepunctureis decidedafter
feeling the vein.
 Swabbing the veinepuncturesite in small outward
circlewitha disinfectant& Do not touchafter
disinfectingthe skin.
 Inserttheneedle intothe vein. Needle shouldbe at
the angleof 30 degree.

8.  The volumeof bloodsampleshouldbe goodquantity.
 The vacationeris to be gently inverted5-10times
 Afterdrawingthe bloodsamples, releasethe tourniquet.Removethe needle
fromthe vein.

9.  Coverthe puncturesitewitha cottonswab, and foldthe patientarm for 1-
2 minutes.
 Removethecottonswabafter2 minutes& coverthe puncturesitewith
BANDAID.

10.  Discardthe needle in sharpcontainer.
 Removethehand gloves& discardin redcontainer.

11. SAMPLE RECIEVING
 Removethe samplefromthe rack or fromthe bag.
 Checkthatthe tubeis firmly stopperedand checkfor any leakageof the
specimen. If thereis, handle as describedbelow

12.  Readthepatient'sfull name fromtherequestformand checkthat the
sampleis labeledwiththesamename. If the sample is unlabelledor has a
differentnameto that on the requestform, treatas a rejectedspecimen

13.  If the labelingis satisfactory, recordthe detailsin the day-registerand assigna
uniquelaboratorynumber;thisnumbermustbe recordedin the register, on
the specimentubeandon the requestform.

14.  BEFOREPERFORMINGTESTRecheckthat the
sampleis correctlylabeledwiththe patient'sname,
thatthe laboratorynumberhas beencorrectly
assigned, andthatthe specimentubeand request
formbear the same number.

15. Hemolysis
 BREAKDOWNOF RBC& RELEASETHERE
CONTENTS(HEAMOGLOBIN) INTOTHE SURROUNDING
FLUID.

16. Wrongvein selection,
contamination, and incorrect
needle size, as well as improper
tube mixing andincorrectly
filledtubes areall frequent
causesof hemolysis
CAUSES OF HEMOLYSIS.