The document reports on a study measuring blood pressure and related metrics in wild-type mice and mice lacking the arginase II gene. Key findings include significantly higher systolic, diastolic, and mean blood pressure as well as heart rate in arginase II knockout mice compared to wild-type mice. Several other measurements including tail blood flow and volume were also significantly higher in the arginase II knockout mice.

1. Blood pressure measurement
WT vs ApoE-/-+HCD
* p<0.01
*
*
*
*
*
*
Day Night
A

2. Day Night
* *
* *
* *
*
* p<0.01
B

3. Blood pressure measurement
WT vs ArgII-/-
* p<0.001
WT ArgII-/-
Day Night Day Night
SBP, mmHg 140.79±0.78 136.84±0.81 164.16±0.32 * 158.17±0.29 *
DBP, mmHg 100.79±0.34 91.62±0.42 121.68±0.96 * 118.15±0.66 *
Mean BP, mmHg 113.82±0.03 102.58±0.58 135.51±0.74* 131.14±0.35 *
Heart rate, bpm 518.70±7.66 519.77±8.62 539.31±1.60 * 544.00±11.97 *
Flow, μL 7.81±0.97 8.79±0.57 13.11±0.33 * 10.09±0.56 *
Volume, μL 23.80±1.62 32.88±2.10 50.75±1.44 * 35.93±2.58 *
Table 1: SBP, DBP, Mean BP, Heart rate, Tail blood flow and Tail blood volume in Arginase
II Knockout (15.32±0.94g) and Wild-type Mice (16.52 ±0.32g) at 8 Weeks of Age
Values are means ± SE; n = number of mice, *P<0.05 using unpaired t test between arginase II KO and WT mice.
Values are means ± SE; n = number of mice, *P<0.05 using unpaired t test between arginase II KO and WT mice.
Values are means ± SE; n = number of mice, *P<0.001 using unpaired t test between arginase II KO and WT mice.

4. pan-actin
Arginase II
WT A2KO
Arginase activity, NO, ROS, Western Blot
*
*
*
A
B
C D

5. Vascular tension measurement
WT vs ArgII-/-
*
*
*
* p<0.001
A B
D
C
E F