Bio reactor defination

1. PRESENTED BY
NAME: SAMIRAN RAJKHOWA
SEMESTER: B.Sc 5th SEM
ROLL NO : 64
DEPERTMENT OF BOTANY

2. CLASSIFICATION OF BIOREACTOR
PROCESS
COMPONENT OF BIOREACTOR
TYPES OF BIOREACTOR
APPLICATION

3.  A Bioreactor may refer to a device or system
meant to grow animal cell tissue in the context
of cell culture.These devices are being
developed for tissue engineering or
biochemical engineering.

4.  In term of process requirments they are of
following types –
 AEROBIC
 ANAEROBIC
 IMMOBILIZED CELL BIOREACTORS

5.  On the basis of mode of operation , it may be
classified as –
• Batch
eg : stirred tank bioreactor
• Fed batch
eg : fluidized bed bioreactor
• Continuous
eg : an exmple of a continuous bioreactor is
the chemostat

6. 1. Agitation :
• Agitation is required for homogenous distribution of cell and
nutrient media in the cells.
• It can be done by magnetic stirred, turbine impeller, marine
impeller.
• Maximum stirring rates for suspension – 100 to 150 rpm.

7. 2. AERATION :
• Through bubbling air.
• Silicon tubing – highly gas permeable ( inconvenient to use)
• By medium perfusion-medium is continuously taken from culture vessel ,passed
through oxygenation chamber.
3. TEMPERATURE :
• Usually set at the same point as the body temperature of the host from which
the cell obtained.
• Temperature maintained by use of carefully calibrated and frequently checked
incubators.

8. 4. pH :
• Most cells in culture grow best at pH 7.4 .
• Keep the pH medium in range 7-7.4 .
• Done by using an incubator with CO2 control set to provide an atmosphere with
between 2% and to 10% CO2
5. STREILIZATION :
• Heating
• Radiation
• Chemicals
• Filtration
6.SCALE UP :
 Scale up means to increase volume of culture .
 It provide the closer prediction for the large scale production about
various factors like ph , temp , aeration , media

9. i. Stirred types of bioreactors
ii. Air lift bioreactor
iii. Fluidized bed bioreactor
iv. Tower bioreactor
v. Continuous bioreactor
vi. Gaseous phase bioreactor

10.  Genetic engineering
 Cell therapy
 Model system
 Viral vaccines
 Monoclonal antibodies
 Recombinant proteins
 Cancer research
 Toxicity testing
 Drug screening and development.

11. THANK YOU