organ regeneration through embryonic stem cell

1. ORGAN REGENERATION
GROUP-
SOURAV DAS
DISHAREE CHAKROBORTY
REGENERATON THROUGH EBRYONIC STEM CELL

2. EMBRYONIC STEM CELL
Also known as a human pluripotent stem cell, one of the cells
that are self-replicating, are derived from human embryos or
human foetal tissue, and are known to develop into cells and
tissues of the three primary germ layers.
It’s self-replicating,which means the cell can divide and form
cells indistinguishable from it. The "three primary germ layers" --
called the ectoderm, mesoderm, and endoderm -- are the
primary layers of cells in the embryo from which all tissues and
organs develop.

3. T.S OF UMBILICAL CORD

4. A large number of stem cells are normally found in the
blood of newborn babies. After birth, the blood that is left
behind in the placenta and umbilical cord (known as cord
blood) can be collected and stored for later use for a stem
cell transplant. The cord blood is cryopreserved until
needed for reuse,sometimes for lifetime.
These stem cells are stored at a temperature of -196
degree Celsius in vapour phase of liquid Nitrogen.

5. STORAGE
THE UMBILLICAL
CORD WILL BE
CLAMPED ANDCUT
CORD BLOOD WILL
BE DRAWN FROM
THE CLAMPED CORD
INTO A SPEACIAL
COLLECTING BAG
THE
COLLECTED
BAG WILL BE
SENT TO CSI
LAB SAFELY
ALL THE
NECESSARY
TESTS WILL BE
PERFORMED TO
ANALYSE THE
BLOOD
STORE THE
CORD BLOOD
CELL INTO
ETHER BAG
THE CORD BLOOD
STEM CELLS WILL
BE KEPT INTO
LIQUID NITROGEN
STORAGE TANKS

6. DEVELOPMENT
When cultured in suspension without antidifferentiation
factors, embryonic stem (ES) cells spontaneously
differentiate and form three-dimensional multicellular
aggregates called embryoid bodies (EBs).
EBs recapitulate many aspects of cell differentiation during
early embryogenesis, and play an important role in the
differentiation of ES cells into a variety of cell types in vitro.

7. There are several methods for inducing the formation of
EBs from ES cells. The three basic methods are--
•liquid suspension culture in bacterial-grade dishes
•culture in methylcellulose semisolid media
•culture in hanging drops.
For the production of large numbers of EBs, stirred-
suspension culture using spinner flasks and bioreactors is
performed.

8. LUNG->The regenerated lungs transplanted into orthotropic position.
After transplantation,constructs were perfused by the recipient’s circulation
and ventilated by means of the recipient’s airway and respiratory muscles.
This provided gas exchange in vivo for upto 6 hr after extubation.