Check the liver diseases, etc.,

1. SUBMITTED BY
K.SANJAY
BD230907
PROTHROMIN TIME AND PARTIAL
PROTHROMIN TIME

2. PROTHROMIN TIME
• The prothrombin time is the time required for
the plasma to clot after addition of tissue
factor (thromboplastin) and an optimal
concentration of calcium.
• This indicates overall efficiency of extrinsic
pathway.

3. PROTHROMBIN AND PARTIAL
PROTHROMBIN TIME
• The prothrombin time (PT) test and partial
thromboplastin time (PTT) measure how
quickly blood clots.
• The tests can help detect bleeding disorders,
check a person's response to blood thinners,
and assess a person's risk for bleeding prior
to surgery.

4. REQUIREMENTS
• Water bath(37°C)
• Stop watch
• Test tubes
• Tissue thromboplastin
• 0.15 gm/dl Calcium chloride
• Platelets poor citrated plasma (PPCP) (mix 0.2
ml of 3.8% tri-sodium citrate with 1.8 ml of
Plasma) prepared after centrifuging citrated
blood at 3000 RPM for 15 minutes.

5. PROCEDURE:
1. Take 0.1ml plasma in clean test tube. The
plasma should be pre-warmed at 37°C in
water bath.
2. Add 0.1 ml tissue thromboplastin, mix well
and incubate at 37°C for 2 minutes.
3. Add 0.1 ml calcium chloride solution, mix
and start the stop watch.
4. At the first appearance of a fibrin clot, stop
the watch immediately.

6. 5. Record the time.
6. Report prothrombin time in
seconds.

8. NORMAL RANGE
• 12-15 seconds

9. INTERNATIONAL NORMALIZED RATIO
• INR = (PT ratio)
• Normal range of INR= 1.0-1.2
• PT ratio= PT of test ⁄ PT of control

10. HIGH PROTHROMIN TIME MEANS
• Liver diseases
• Lack of Vitamin K
• Genetic Disorder that you inherit from your
parents

11. THANK
YOU