Antibody markers and tools for analysis

1. ANTIBODY MARKER
BY PUJA BASURI
SCHOLAR NO- 222124109
MTech 2ND SEMESTER (BIOTECHNOLOGY)

2. INTRODUCTION
PRESENCE OF DISEASE OR SPECIALISED CELL

3. • Human chorionic gonadotropin
(hCG)
• Prostate-Specific Antigen (PSA)
• Antinuclear Antibodies (ANA)
• Rheumatoid Factor (RF)
• C-reactive protein (CRP)
• Immunoglobulin E (IgE)

4. TECHNOLOGIES USED
• Enzyme-Linked Immunosorbent Assay (ELISA)
• Western Blot
• Immunofluorescence
• Lateral Flow Assay
• Immunohistochemistry

5. ANALYSING
Sample collection
Antibody detection
Interpretation of results
Correlation with clinical symptoms
Follow-up testing

6. APPLICATIONS
• Diagnosis of infectious diseases
• Monitoring immune status
• Research
• Drug development
• Forensic analysis

7. ADVANTAGES
• High specificity
• Sensitivity
• Versatility
• Non-invasive
• Easy in performance

8. DISADVANTAGES
• Cross-reactivity
• Limited availability
• Limited shelf life

9. ONGOING RESEARCH
• COVID-19
• Cancer immunotherapy
• Alzheimer's disease
• Autoimmune diseases
• Botulinum neurotoxin

10. REFERENCES:
• Gohain, N., Tolbert, W. D., Van der Jeught, K., Das, J.,
Demeyer, A., Bai, Y., ... & Verhaegen, M. (2021). Enhancing the
potency and specificity of engineered T cells for cancer
treatment. Cancer discovery, 11(2), 346-359.
• Smith, M. J., Okoli, A., Hernandez, I., Yao, J., & Paton, A. W.
(2021). Development of a multiplexed biosensor assay for
simultaneous detection of botulinum neurotoxins. Analytical
chemistry, 93(17), 6809-6816.
• Arunachalam, P. S., Wimmers, F., Mok, C. K. P., Perera, R. A. P.
M., Scott, M., Hagan, T., ... & Bristow, L. (2020). Systems
biological assessment of immunity to mild versus severe
COVID-19 infection in humans. Science, 369(6508), 1210-1220.

11. THANK YOU