1. Preventine Ltd – UK
We have pleasure to provide a brief overview of our Preventine MLC Larvicide
for the reduction of Malaria, Dengue, Chikungunya, Cholera & Typhoid
The answer to Pyrethroid resistance build up on millions of Bed-nets ?
(A unique larvicide - with a difference)
Preventine MLC is a complex saturated ionic aqueous solution containing
minerals such as Gold, Silver, Copper, Aluminium and Zinc, (avoiding known
chemicals used on some treated bed-nets, insecticides and larvicides against which
mosquitos have developed a resistance to - see VI below)
The microscopic action of these natural mineral ions :
I) break down the cell walls of Anopheles, Aedis Aegypti and Culex mosquito
larvae and destroy the DNA structure, arresting the breeding cycle at instar
1-4 and pupae stage.
II) break down 7 waterborne bacteria which once destroyed through change/
transition of their enzyme pathways (achieved within 3 hours of detention time at
a 1:1000 dilution rate of MLC with water)
III) achieve a remarkable reduction/elimination of Odor within that detention time.
Odor over stagnant water, land-fills and open sewers are attractive to mosquito
sensory systems to deposit eggs, but also to the common house fly.
Ammonia NH3 is one of the most offensive odors, specially at intensive pig farms.
At 1000 ml of pig effluent and 0.9ml in quantity NH3 was eliminated.
Preventine MLC is not produced as treatment for Drinking water.
Pure Pig manure - 211 trial is treated.
Water clarity after 72 hours detention
time.
<<<<<<<<<<
Kheshie Lagoon Accra - human
effluent
(mainly from Storm drains as
sanitation is lacking - dark bucket)
4 hours - detention time >>>>>>>>

2. Stagnant ponds, blocked drains, landfills (decaying food remains), discarded tires, pots
pans are the habitat for oviposition.
We reduce odor, achieve total kill of Anopheles,
Culex, A.Aegypti species Instar 1-4 + Pupa, eliminate
house flies from completing the life-cycle, kill
Coliform and bacteria in water touched by humans in
one spray action.
Human and/or farm live-stock effluent consists to a
level of 80-90% of water. Lagoons treated wih
Preventine MLC produce clear water as solids
separate and sink to sludge level. Pure pig manure in
jars. Clear water after detention time.
Such water is good enough for agriculturalal irrigation
purposes, whilst the sludge is enriched with minerals for
subsequent use to mix with soil, peat, sand as fertiliser.
(Please refer to the extract of Dr. Lory’s peer supported
study - Appendix page 8)
Floc / E-coli tests
Pure pig manure
after
6 Hours detention time
Same sample test 211
after
24 hours detention time
Kpeshie Lagoon test sample
1. “organic matter” highest dose of
Ecoli 0157H7 registered. (Cholera)
2. Water clearing top down after 4
hours detention period

3. IV) Eliminate Coliform (E- Coli colony counts) for fecal contamination of water,
including the deadly Escherichia Coli 0157:H7 strain, including Typhoid - see
extract below.
Agar Diffusion test (Well Variant) Inhibiting the growth of 7 pathogenec bacteria.
Test organisms
Zones of inhibition (mm) of MLC against test organismsZones of inhibition (mm) of MLC against test organismsZones of inhibition (mm) of MLC against test organismsZones of inhibition (mm) of MLC against test organismsZones of inhibition (mm) of MLC against test organismsZones of inhibition (mm) of MLC against test organismsZones of inhibition (mm) of MLC against test organismsZones of inhibition (mm) of MLC against test organismsZones of inhibition (mm) of MLC against test organisms
Test organisms 1% 2% 5% 10% 20% 40% 60% 80% 100% MIC
E. coli R R R R 30 30 30 30 30
20%
K.
pneumoniae
R R R R 30 30 30 30 30
20%S. typhi R R R R 30 30 30 30 30 20%
P. mirabilis R R R R 30 30 30 30 30
20%
E. faecalis R R R R 30 30 30 30 30
20%
P. aeruginosa R R R R 30 30 30 30 30
20%
V. cholerae R R R R 30 30 30 30 30
20%
R = Resistant
Preventine MLC is not produced as treatment for Drinking water.
Middle dish = Control
untreated

4. V) House flies in tropical urban or village environments lacking sanitation.
Of significance to humans is that common flies are also prolific carriers of disease and
thrive in environments where poor sanitation, standing effluent, provide excellent
breeding habitat. Moreover, the temperature and humidity levels common to villages
and shanty towns in tropical climates are often optimum for fly reproduction and
survivability. In fact, A pair of flies beginning reproduction in April may be progenitors,
under optimal conditions and if all were to live, of 191,010,000,000,000,000,000 flies by
August. While this potential reproductive capacity of flies is statistically possible, it
cannot be realized in nature.
Nevertheless, with that potential growth in population simply as a reference, even a
small percentage survival rate from these TWO adults will leave millions of their progeny
to carry the necessary pathogens to bring sickness, or worse, to a village with poor
sanitation and raw sewage. Minimizing the number which survive to adulthood and
reproduce in these habitat will have a profound impact on human health and life in these
villages and shanty towns plagued by insect borne disease transmission.
Extract of study: Timed Larva Development and Survival Data
(misted water = Preventine MLC 1ml concentrate /1liter dilution rate.
Larva and pupa in the control chambers continued to develop with approximately 80% surviving to
adulthood.
While it appeared that all larva and pupa were dead within 24 hours after treatment, it was clear that
Preventine MLC caused complete mortality in the treatment chambers in comparison to the normal
development observed in the control chambers.
Chamber
number
First Pupal Stage
Observed in >10%
of larva at
indicated time
lapse
Treatment after
indicated
cumulative time
lapse of 08:00:00
Percent larva
entering first pupal
stage after
cumulative time
lapse of 09:00:00
Percent larva in
first or later pupal
stage after
cumulative time
lapse of 13:00:00
Percent Adult Flies
emerging from
pupa after
cumulative time
lapse of 20:00:00
Percent Larval
mortality after
cumulative time
lapse of 20:00:00
S1C01
(200 Larva)
07:00:00 Misted water 60% 75-80% 80% 20
S1C02
(200 Larva)
07:00:00 N/A 60% 75-80% 80% 20
S103
(200 Larva)
07:00:00 Misted MLC 20% 20% 0 100
S104
(200 Larva)
07:00:00 Misted MLC 20% 20% 0 100
EDUCATION is essential !
Examples The small waterbaggies are sold by
ladies in the road. Assume that they have not washed
their hands in hot water and soap but you need to
open the bag with your mouth.
Drinking water taken from a river where cattle bathe.
Health risk : Cholera

5. Preventine MLC is not produced as treatment for Drinking water.
We provide a manual during thorough training for spray team supervisors to identify the species / sub-
species. It teaches basic life cycle, identification of egg clusters, larvae position in water, important genera
and the diseases they transmit via Anopheles/Aedes/Culex species: Malaria, Dengue, Filariasis, Yellow
Fever.
The area of test procedures are documented with GSM accuracy to compare the attendance of Malaria
cases at regional hospitals after a season.
Whether in the field or presentations, we explain the tools availble for intergrated vector control and the
“add on” values we can deliver concerning waterborne Pathogens.

6. Very large expanses of effluent from humans and/or Farm stock
Large Refugee camps (Southern Sudan), wetlands, open mining sites in the rainy
season, Dam construction can best be served by an air/truck transportable Hovercraft.
We recommend the EPS 2.2 prduced by EPS Corp in the USA that can take 1.5 tons of
MLC / Water mix to spray and flies at 0.70 cm over obstacles on land and water.
Kpeshie Lagoon - Accra
Grass adjacent to Mangroves provides an ideal
breeding habitat (low level clean water) for
Anopheles Gambiae that swarm in millions.
Dirty water- with Effluent. If we request our
people to conduct scooping tests, MLC is sprayed
on hands before taking local food

7. VI) PYRETHROID on bed-nets has caused a total resistance build up in many regions.
The PMI (Presidential Malaria Initiative) lauched under President Bush, announced
proudly the delivery of the 1 BILLIONth bed-net.
There is no doubt that Bed-nets have over many years reduced worldwide
Malaria deaths by some 20% but are we risking to reverse the gains achieved.
In the view of many medical Entomologists on the ground, this is already reflected in a
drop of efficiacy of insecticides and larvicides now in use.
We are able to step up dilution from 1ml/Liter to 2ml/Liter to meet this serious threat.
We have introduced in Ghana a club, run by women, to use untreated
netting, cut from 54” rolls for window, door frames and chicken coops.
Latter are essential in villages where nourishment is taken from the
forest, but chickens die when infestation of mosquitoes is high.
Protein from Eggs and Chicken meat compliments the diet.
Foundations offer donations for flat-pack construction.
The women have an income which provides in an African society status in the family.
(Empowerment of Women in an African culture)
For our Pilot schemes we fly the flag for Ghana, the gateway to Africa.
Preventine MLC is not produced as treatment for Drinking water.

8. Samples taken from Kpeshie and Korlebu Lagoon, Accra confirmed extremely high
levels of Coliform (E-coli) We carry a chemical test kit for the sake of our employees
who are asked to conduct larval scooping tests to assess infestation rates.
Preventine MLC concentrate can be delivered by courier services in case of urgent
need as a non hazardous shipment in accordance with 94/112/EEC and ISO 11014-1 from
the United Kingdom together with user instructions and a Material Safety Data Sheet.
The liquid is available in bottles of 100 ml for trials (subject to exchange of an NDA)
1 Liter, 5 Liter bottles and 25 Liter drums - The product has a shelf life of 5 years.
Our company delivers, if so requested, the complete kit: spray equipment and personal
clothing for operators, specialist delivery vehicles etc. Please contact us for any
enquiries or questions you may have.
W. Africa: Contact for Ghana
Tel : 0553732243
Mr. Samuel Krakani : skrakani.preventine@gmail.com
Madam Vera: veetornam.preventine@gmail.com
Private E-mail: PT-Consultant@hotmail.com
Peter Troniseck, Chairman peter@preventine-ltd.com
International Sales office :
P.O.Box 4936, Poulters Lane,
Worthing, W. Sussex
England - BN11 9YE
Central E-mail: info@preventine-ltd.com
Admin/Logistics +44 (0) 1903 266 697
Ian Hackett ian@preventine-ltd.com
Sales +44 (0)1425 476 389
Barry Burns Barry@preventine-ltd.com
Central E-mail address info@preventine-ltd.com
Preventine Ltd. Registered in England No 7859616

9. Appendix : Preventine MLC used on Plants, soils or as sludge.
Effects of Copper & Zinc for use of MLC on agricultural land and sludge.
We were made aware of concerns and requested a peer-supported study by the
Life Science Dept. of the Missouri University USA. For an extract, see below. A full
copy by the lead scientist Dr. John Lory is available on request.
1. COPPER : This material is substantially lower in copper concentration then many copper
fertilizers!
One would need to apply 750L/ha of the undiluted product to approach an application rate of
15kg/ha (the approximate annual application maximum for copper in sewage sludge for sandy
soils) Atmospheric deposition of copper exceeds the rate this material is likely to be applied to
soils in many parts of the world.
Application in excess of 5L/ha per year of the undiluted liqid to exceed 0.1kg copper ha / year
2. ZINC.
The undiluted concentrate liquid has a zinc concentration of just under 0.7% which should be
diluted (as directed 1:1000) before direct application to living plants but would cause no danger
to plants if applied direct to the soil.
This material is substantially lower in zinc concentration then many zinc fertilizers. The diluted
product is unlikely to significantly zinc loading in soils. One would need to apply over 4000 L/ha
of the undiluted product to approach an application rate of 28Kg/ha. Atmospheric deposition
of zinc exceeds this material applied to soil in many parts of the world.
One would need in exces of 72L/ha per year of the undiluted material to exceed 0.5 Kg zinc/ha
per year
Preventine MLC is not produced as treatment for Drinking water.