There are several options available to generate stable cell lines depending on the purpose of the experiment. Culture conditions (a passage, split rhythm, number, etc.) Stable cell lines can only be created if you have the right cell type.

2. There are several options available to generate stable cell
lines depending on the purpose of the experiment.

3. Culture Conditions
 Culture conditions (passage,
split rhythm, number,
etc.) Stable cell lines can
only be created if you have
the right cell type.

4. Design experiment
 Create an experiment and
select the expression
vector, cell type, and
transfection
method. Check that the
expression vector and
transfection methods you
choose are appropriate for
your cell type.

5. Select the G418 concentration and
the cell number
 Matrix titration can be used to determine the
appropriate G418 concentrations and cell numbers
per well. Attention to the fact that stock G418's
active concentration can vary from batch-to-batch.

6. Transfection
 Transfect expression
plasmid
manufacturing to
cells. Follow the
instructions provided
by your transfection
device
manufacturer. G418
should not be added
to the culture medium
right away after

7. Cell selection
 Transfect cells with G418 and culture them in medium
containing the appropriate amount of G418. You will
get a mixed population of drug-resistant cells.

8. Analyze
 You should choose the
right method to analyze
your stable-transfected
cells.

9. Thank You
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