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Nitin Dhore
V/16/258
DEPARTMENT OF POULTRY
SCIENCE
Advances in Pullet Management
KRANTISINH NANA PATIL COLLEGE OF VETERINARY
SCIENCE SHIRWAL, DIST. SATARA (MS)
MASTER CREDIT SEMINAR
PSC: 691 (0+1)
ON
MAHARASHTRA ANIMAL & FISHERY SCIENCES UNIVERSITY, NAGPUR
Introduction
Pullet Development
Nutritional Intervention
Managemental Intervention
Genetic Intervention
Conclusions
Future Prospectus
Statistics
(Basic Animal Husbandry and Fisheries Statistic 2022)
2.43% of the World
poultry population
Present in India
The Indian poultry industry:
ranking 3rd in egg
production Growth rate 8
to 10%
Production of 129.60
billion eggs in 2021-22
Total population: 851.81
million (India)
Maharashtra has 74.3
million (Ranked 5th )
Pullet?
Karcher et al. (2018)
• Female that have not laid an egg are referred to as
pullets. The term pullet is used until approximately 18
weeks of age, at the point egg production begins.
• The pullet developed according to a
well-orchestrated sequence of
physiologic events.
• Nutrition is the key to optimal
genetic performance.
• Interrupted growth during this developmental phase: results in
lacks of body reserves and organ function fail to sustain high
production.
Fast intestine
development
Fully
developed
intestine &
immune
system
6 12
Fastest skeletal
growth
90-100g BW/wk
95% of
skeleton
development
Reaches to
95% of adult
size but only
75% of mature
weight
Muscle growth,
medullary bone &
reproductive tract
development will
continue much of
WG
Full mature BW
achieved
Complete
transition of
skeletal bone to
building medullary
bone.
Estrogen
stimulates oviduct
development
2-3
wk
To aid medullary bone
growth before 1st egg , it
is recommended to
introduce more Ca
through PRELAY DIET
Pullet Development
18
16
Lonkar et al, (2022)
6
Skeletal problem.
Low body weight.
Variation in flock.
Cage fatigue.
Low body reserve.
Poor feather Cover.
Poor performance.
Delayed sexual maturity.
Uniformity flock.
Correct body reserve.
Medullary calcium
reserve.
Correct age at sexual
maturity.
Good body condition.
Skeletal development.
Good Layer
Improve Body Reserve
Sustain egg production.
Better egg quality.
Ability to lay extended laying
cycle.
Good welfare.
Increase per capita
availabilty.
Extended laying cycle result
into pullet cost dilution
Fuel
Poor Pullet
Good Pullet
7
Nutrition
Dietary ME &
CP
Dietary fat
Prelay
Restricted
feeding
Management
Lighting
Vaccination
Stocking density
Biosecurity
Temperature &
Humidity
Genetics
Intensive selection
of trait
Breeding
programme
Fuel
Importance of Pullet Nutrition
Body weight
Body composition
Medullary bone
development
Uniformity
Feather cover
• Pullet feed contributes indirectly to egg production by influencing sexual maturity,
live weight and body composition at the onset of lay.
• Optimum feeding management practices followed during the rearing period in pullets
have effects on body weight, uniformity, egg production, egg weight, egg quality, and
hatching properties (Sarica et al., 2009).
• Growing period of a laying hen is the most critical time in a hen’s life and the
mistakes made during this period are difficult to rectify.
8
Pullet Nutrition
Importance of Pullet Nutrition
• Elevated Ca: P ratio in pullet and prelay diets decreased femur-breaking strength at the
end of layer phase without affecting pullet BWG and pullet feed efficiency in the cage
system.
• Prelay diet containing 2700 kcal ME/kg and 18% CP for pullets before sexual maturity to
improve body composition and medullary bone development.
• Insufficient dietary Ca during rearing period has an adverse effect on eggshell quality and
bone strength.
• Protein intake during pullet-to-breeder transition period: improve early EW and EP by
increasing CP levels of in prelay diets.
9
Joseph et al. (2000)
Whitehead(2004)
Sujatha et al.(2013)
Fosnaught(2009)
Effect of Dietary CP and ME Levels During Growing Period on Early
Egg Production
Feeding the higher protein levels (P2 and P3) had the highest hen-day egg
production and egg mass. The feeding of the pullets with P3E3 diet during the growing period
will give best hen-day egg production.
Babiker et al. (2010)
N=270,9T,3R(10/R)
Experimental period was divided into four phases. In all phases of growing period, control
levels of protein (P1) and energy (E1) were set according to NRC (1994) and the other levels
were higher, for protein in 2% steps (P2 and P3) and for energy by 100 kcal of ME/kg steps
(E2 and E3). 19th wk onward commercial layer diet upto end of trial.
Hen Day Egg
Production (%)
Energy Hen Day Egg
Production(%)
Protein Effect ** Energy Effect NS
P1 18-16-15 50.1b E1(3000-3000-3050) 54.2
P2 20-18-17 56.1a E2(3100-3100-3150) 54.5
P3 22-20-19 58.8a E3(3200-3200-3250) 56.3
Age effect **
22 16.3c
24 51.5b
28 70.0a
32 71.3a
36 65.8a
10
Growth and egg production of layer pullets can be affected by the
method of supplying energy and protein sources
N=320, 9woa brown egg laying strain pullet (ISA),4T control (balanced diet);
E60:P40,E40:P60,EPFC (free choice between ES and PS in separate troughs) 4R(20/R).11 h of
light at the initiation of the experiment and reached 14.5 h of light at 21 weeks of age. Egg
laying start lighting programme was gradually increased from 14.5 to 16 h of light upto 33 wk
of age.
Ocak et al.(2009)
• Feeding PS during 60% of the daily photoperiod increased egg production by improving
the feed efficiency compared with other treatments.
Parameter Controla E60:P40 E40:P60 EPFC SEM
Hen-day egg production(22-33wk)
HDEP% 87.3ab 84.3b 88.4a 86.0ab 0.59
Egg weight
(g)
63.9 63.9 63.5 63.0 0.22
11
Effect of Dietary fat on pullet developement
The most important factors determining egg weight is body weight of hen at the
age of sexual maturity.
Summers and Leeson (1983)
Increasing dietary energy or dietary fat significantly increases early egg weight due
to increased in Yolk weight.
Wu et al. (2005)
Birds receiving 4% corn oil (2.08% linoleic acid) produce numerically larger eggs,
best feed efficiency and gain slightly more BW as compared to birds receiving 0%
corn oil.
Najib and Sullivan (1986)
12
Palm Oil and Animal Fats for Increasing Dietary Energy in Rearing
Pullets
Isika et al. (2006)
N=300, 5 T, 3R(20PULLET/R),(NERA STRAIN PULLET),Birds fed grower diet containing
0%(control),2.5 and 5 % palm oil, 2.5 and 5% broiler fat. From 22nd woa commercial layer diet
was fed.
13
Ingredients Palm Oil Broiler Fat
PO/BF inclusion (%) 0.0 2.5 5.0 2.5 5.0
a. Ages: Week At 1st egg 18b 18b 19a 19a 18b
ii. Hen-house egg production 67.8c 70.5ab 73.3a 67.3c 71.2a
d. Egg qualities I. Size
(g/egg)
50.4b 51.3b 57.1a 54.0b 53.1b
ii. Shell thickness(mm) 0.34 0.33 0.34 0.333 0.32
5% inclusion of palm oil and broiler fat higher hen housed egg production and had higher
egg weight.
Egg Size of Saudi Local Layers as Affected by Dietary Fat Level
Huthail Najib and Yousif Al-Yousif (2014)
Linoleic acid play an important role in maximizing the egg size of the local birds by 4% corn oil.
It is a good way of improving the egg size of the Saudi local brown birds during the onset and
first part of the laying year.
14
N=120, 2*3 FACTORIAL DESIGN (black, light (700-900 gm) & Brown, medium (900-1080
gm).* Corn oil, 0, 2 and 4%, 6T(20/T), 4R(5/R) at the age of 22 weeks.
Source of
variation
HD EM EW
Levels NS NS **
0 48.26a 22.52a 46.47a
2 48.02a 22.82a 47.29b
4 49.77a 24.68a 48.92c
p = 0.7535 0.1734 <0.0001
Adequate reserve in medullary bones at beginning of the production cycle is crucial in
reducing cage fatigue, in maintaining a good EP and formation of eggs with a good eggshell
quality
Moderate Ca levels (1.5-2.75%) fed during the prelay period allows for the buildup of the
medullary bone reserves of Ca without adversely affecting kidney function and may have an
effect on shell quality in subsequent laying cycle.
A prelay diet is introduce before the commencement of EP is an extremely important period
in the life of a high genetic potential layer.
Good body reserve (fat and protein), positive energy balance and Ca reserves in the
medullary bone at the onset of production is essential for sustained EP and egg size
(Miles & Jacqueline, 2008)
(Williams et al., 2000)
(Sujatha & Rajini, 2015)
Prelay Diet
Prelay feeding changes the pullet body composition
N=360,5 pre-lay diet, 6R(12/5),fed during 15woa to sexual maturity in experiment 1.
In experiment 2. Best two nutrient compositions with respect to body composition of
pullets from Exp.1 and fed with 1,1.5, and 2% Ca.
Sujatha et al.(2013)
Treatments (ME/CP) Crude Protein % Fat %
2500/16 21.54b±0.25 7.31b±0.09
2700/16 21.34b±0.15 11.29a±0.35
2700/18 24.38a±0.48 11.44a±0.14
2700/16+10% L+M 21.34b±0.15 11.29a±0.35
2700/16+10% L+M + 2% oil 21.54b±0.31 13.32a±0.05
• It could be concluded that pre-lay diet containing 2700 kcal/kg of dietary energy, 18 per
cent CP and 2 per cent calcium is advisable for pullets before sexual maturity
16
Nutrient density in Prelay diet affect the laying performance
Sujatha et al,(2014)
Pre-lay
treatments
Control-2500/16* 2700/16% 2700/18* 2700/16 (10% L&M)* 2700/16 (10% L&M +2% oil)*
Hen day egg production (eggs hen/d)
Age in Weeks Standard layer feed
20-23NS 0.434C (± 4.46) 0.465C (± 4.89) 0.519B (± 5.37) 0.466C (± 3.64) 0.469C (± 4.99)
24-27* 0.725B,c (± 0.99) 0.793B,b (± 1.81) 0.904A,a (± 1.04) 0.789B,b (± 1.86) 0.734B,c (± 1.80)
28-31* 0.788A,c (± 0.50) 0.851B,C,b (± 1.76) 0.946A,a (± 135) 0867A,b (± 1.85) 0841A,b (± 1.71)
32-35* 0.795A,B,c (± 2.83) 0.935A,a (± 1.43) 0.912A,a (± 1.36) 0.879A,a (± 1.67) 0.877A,a (± 1.81)
36-39* 0.837A,c (± 1.56) 0.942A,a (±1.81) 0.957A,a (±
0.702)
0.867A,b,c (± 1.53) 0.888A,b (± 1.84)
40-43* 0.765A,B,c (± 1.16) 0.869A,a (± 1.64) 0.886A,a (±
0.796)
0.747B,b (± 1.51) 0.827A,b (± 1.54)
Overall mean* 0.7245c (± 1.40) 0.809b (± 1.6) 0.853a (± 1.52) 0.769b (± 1.40) 0.773b (± 1.52)
Egg weight (gm)
Age in Weeks Standard layer feed
20-23NS 44.86D (± 0.52) 45.55C (± 067) 46.20D (± 0.74) 45.86C (± 0.81) 46.50D (±0.79)
24-27* 50.42C,c (± 0.23) 51.25B,a,b (± 021) 51.34C,a,b (±024) 50.59B,b,c (± 0.36) 5.30C,a (±0.24)
28-31* 54.11B,c (± 0.22) 55.23A,a,b (± 0.20) 56.12B,a (± 0.41) 54.86A,b,c (± 0.38) 55.41B,a,b (±0.28)
32-35* 55.50A,b (± 0.23) 56.99A,a,b (± 1.09) 57.58A,a (± 039) 55.72A,b (±0.29) 56.94A,a,b (±0.31)
36-39* 55.02A,c (± 0.19) 56.58A,b (± 0.31) 57.50A,a (± 0.35) 55.17A,c (±0.18) 56.20A,B,b (±021)
40-43* 54.03B,b (± 0.12) 56.43A,a (± 0.49) 57.22A,B,a (±
0.44)
55.01A,b (±0.37) 56.17A,B,a (±0.25)
Overall mean* 52.34c (± 0.31) 53.67a,b (± 0.39) 54.33a (± 0.37) 52.87b,c (±0.32) 53.79a,b (±0.33)
17
Effects of dietary calcium and phosphorus level on egg shell in
brown egg-type pullets from 0 to 32 weeks of age
N=1380,6T,10R(23PULLET/R), Dietary Ca and P high(7.5-6.7-7.3:3.6-3.2-3.0) and
low(5.8-4.7-5.0:3.2-2.6-2.4)Ca-P) from respectively 0 to 6, 6 to 11,and 11 to 16 wk of age).
WEEKS 22nd 25th 32nd
EFFECT Egg shell
thicknesss
(mm)
Egg shell
thicknesss
(mm)
Egg shell
thicknesss
(mm)
HIGH
(Ca:P)
(7.5-6.7-7.3:3.6-3.2-3.0)
0.401 0.406 0.391b
LOW
(Ca:P)
(5.8-4.7-5.0:3.2-2.6-2.4)
0.397 0.402 0.402a
Egg shell thickness at 22, 25, and 32 wk of age, fed diets during rearing (0 to 16 wk of age) and high or low Ca and P level.
• Low dietary Ca-P during rearing had no clear effect on pullet development and egg
production, but some improvements on egg shell quality were identified at 32 wk of age.
Dijkslag et al.(2021)
18
Targeting the Pullet Body Weight for efficient layer performance
19
Most important factors determining egg weight is body weight of hen at the age of
sexual maturity.
Oke et al. (2004)
Hens with low or average initial body weight had higher EP and EW than the lighter hens.
Perez-Bonilla et al. (2012)
BW and BC play an important role in sexual maturation and egg production in laying hens.
Hanlon et al. (2021)
N=96 shaver brown 6th wk of lay, 4T(24/T) (T1= 0.9-1.2kg, T2= 1.3-1.4kg, T3=1.5-1.6kg and
T4= 1.7-2.0kg). 4R(6/R).
Edeh et al.(2020)
The effect of bodyweight variation on laying performances of
Shaver brown hen in humid tropical environment
Parameters T1 T2 T3 T4 Sig
Body
Weight(kg)
1.05 1.35 1.55 1.85 NS
Average
Daily Feed
Intake, g
73.50±0.29ab 75.5±0.65ab 69.25±4.19b 78.50±1.32a *
Egg
Weight(g)
61.89±0.62b 63.22±0.64ab 64.62±1.81a 64.65±1.06a *
Hen Day egg
Production, %
73.97±1.07a 74.30±0.81a 70.35±0.35b 69.65±0.67b
20
The light weight hens had the highest yield and less cost of feeding which is more profitable
to farmers.
BODY FAT
Both low and high body fat contents could have a negative effect on the productivity of hens.
21
Hocking et al. (2002)
Body fat threshold between 10% to 15% is required to initiate sexual maturation.
Bahry et al. (2023)
N=45 TETRA SL laying hens,3T.Experiment was started at 20 weeks of age.
• The high initial body fat content of the hens resulted in the lowest egg production intensity
at all examination days. Milisits et al.(2016)
Effect of Low, medium and high body fat composition of pullet on
egg production
22
Egg production(%)
Initial body fat content Level of
significance
(P)
IBF
S.E
Wks Low
22.9%
Medium
24.9%
High
27.2%
32 94.9 96.2 98.3 0.400 0.8
52 96.2b 90.2a 87.1a 0.010 1.3
72 80.0b 76.1ab 73.2a 0.039 1.1
Egg weight(g)
32 63.4 62.5 61.5 0.526 0.7
52 65.9 62.0 64.2 0.112 0.7
72 66.5 65.9 64.6 0.755 0.9
Effect of starting body fat content of laying hens on the egg
production during the first egg laying period
• The starting body fat content of laying hens has significant effects on egg production
Milisits et al. (2015)
TETRA SL Starting body fat content
Low 23.1% Average25..6% High28..4%
Hen day egg production
TETRA SL 332b±13 331b±13 318a±20
Effect of starting body fat content of laying hens on the number of total eggs laid by the two genotypes (mean±SE)
23
N=120,(60 TETRA BLANCA white egg layers and 60 TETRA SL brown egg layers),Pullets
based on their body fat content at 16 weeks of age.
Feed Restriction during Grower phase.
• Manipulate growth and metabolism to get desirable body weight during
laying which in return helps to delay sexual maturity and increases
production performance of the birds without affecting their welfare or
health.
• Increase the overall egg production, settable eggs, and average egg weight,
and enhance fertility, hatchability, and egg quality in broiler breeders.
24
Tesfaye et al. (2009)
Avila et al. (2023)
Effects of feed restriction on production performance of Rhode
Island red pullets
Tesfaye et al. (2009)
Feed restrictions by skip a-day feeding system at middle age of development (T3) resulted in
higher egg weight and this can be considered as the optimum age for pullet rearing.
N=240(48.92 ± 1.53 g),4T, 3R(20/R), 4 feeding treatments [T1 (Unrestricted, Control), T2
(Restricted at 7, 14, 21 and 28 days of age), T3 (Restricted at 35, 42, 49 and 56 days of age)
and T4 (Restricted at 63, 70, 77 and 84 days of age] using (CRD).Commercial starter layer diet
(0-6)weeks and commercial grower layer diet (7-22 woa).
Parameters
Treatments
T1 T2 T3 T4 SE
P-
value
Age at onset of egg
laying (days)
140.80 ±
0.80bc
140.17 ±
2.50c
147.00 ±
2.20a 143.83 ±
2.30b 0.96
*
Body weight (g) at first
egg
1305.00 ±
13.20b
1374.30 ±
23.20ab
1352.00 ±
23.50ab
1429.23 ±
44.50a
15.18 **
Egg weight (g)
42.68 ±
0.72b
42.43 ±
1.91b
47.9.00 ±
0.87 a
41.88 ±
0.77b
0.79 **
Shell thickness (mm) 0.26 ± 0.01ab 0.25 ± 0.00b
0.26 ±
0.01ab
0.29 ± 0.01a 0.01 **
25
Effects of energy restriction during growing phase on the productive
performance of Hyline Brown laying hens aged 6–72 wks
Lu et al.(2023)
Weeks
Contro
l
2,850
Energy-restricted
feeding (6-17 wk)
SEM
P-value
Energy restriction
level
Linear
Quadrat
ic
2,565
2,280
19−20 wk 2.41a 0.45b 0.00c 0.552 0.046 0.038 0.502
21−24 wk 37.94a 31.12a 17.51b 2.540 <0.001 <0.001 0.328
25−28 wk 93.45 94.40 90.94 0.761 0.160 0.173 0.168
29−32 wk 93.34 93.28 96.41 0.692 0.102 0.066 0.254
33−36 wk 93.77b 93.87b 98.35a 0.695 0.002 0.002 0.054
37−40 wk 93.60b 95.61ab 97.14a 0.594 0.039 0.012 0.831
41−44 wk 91.01b 95.45a 96.70a 0.851 0.007 0.003 0.266
45−48 wk 90.57b 93.29ab 94.54a 0.643 0.024 0.008 0.526
49−52 wk 90.67b 92.42ab 94.18a 0.639 0.072 0.024 0.994
53−56 wk 86.88b 90.66a 91.27a 0.727 0.017 0.008 0.224
57−60 wk 82.86b 88.86a 89.78a 1.077 0.008 0.004 0.170
61−64 wk 82.30b 90.10a 90.28a 1.208 0.002 0.002 0.053
65−68 wk 80.00b 90.31a 90.18a 1.408 <0.001 <0.001 0.010
69−72 wk 75.97b 86.72a 86.53a 1.578 0.001 0.001 0.029
26
a-c Means without common superscripts within a row differ significantly (P < 0.05).
27
Pullet Uniformity
Standard for Pullet Management
85% ± 10% Excellent Uniformity
82-85 ± 10% Very Good Uniformity
70% ± 10% Unsatisfactory
The Effects of Dietary Whole Rice Hull as Insoluble Fiber on the
Flock Uniformity of Pullets Laying Hens
N=1500 N Brown Nick pullet chicks of 4 woa (uniform weight) divided into 3T,5R(100/R)
They were provided with ad libitum access to water and fed the basal grower diets (CP, 19%;
ME, 2, 950 kcal kg−1 ) containing 0 (control), 3 and 6% WRH.
Incharoen et al.(2013)
A greater enhancement of growth rate and percentage of flock uniformity were observed in
pullet chicks fed both dietary WRH levels, compared to birds in the control group
Parameters Control 3% WRH 6% WRH
Initial body weight, g (4woa) 526±1.38 521±2.51 527±1.41
Final body weight, g () 1107±4.17b 1129±2.81a 1130±2.51a
Body weight gain, g 580±4.93b 608±5.29a 602±2.65a
Feed intake, g 2063±2.46c 2132±10.50b 2205±16.53a
Feed conversion ratio (FCR) 3.56±0.03a 3.50±0.04a 3.66±0.02b
Flock uniformity
within ± 10% of
each pen mean (%)
85.0±1.70 87.4±1.84 86.8±1.93
28
29
Light Influences physiological responses, behavior, growth and development,
and production performance in poultry.
Blue and green
light
Improve growth, help to calm down, promote immune performance
(Zhang et al., 2014), and improve egg quality (Er et al., 2007).
Red light
Increase the levels of reproductive hormones, promote the development of
sexual organs, influence the age of sexual maturity (SM) of pullets,
improve production performance, and reduces the incidence of feather
pecking and cannibalism.
Blue–Green
light
Stimulated growth of chickens
Orange–Red Stimulated reproduction development (Rozenboim et al., 1999).
Widely applied in poultry production likely do not meet the requirements
for the welfare and health of layer chickens, especially for the brooding
and rearing periods (0–20 wk of age),
White LED and
monochrome
LED lights
Advances in Light Management in Pullets
(Borille et al., 2015)
(Liu et al., 2017)
(Hassan et al., 2013)
30
Wei et al. (2020)
Yellow –Orange
phase 2 (14–20 wk
of age)
Increased the BMD concentrations, promoted sexual organ
(oviduct and ovary) development, advanced the age of sexual
maturation, and improved the production uniformity of layer
chickens
Blue –Green
phase (0–13 wk of
age)
Increased the serum Ig concentrations and serum GLU
concentration levels of layer chickens
Wei et al. (2020)
Typically, pullets are reared under an 8–10 h photoperiod and photostimulated at 16–22
weeks of age with a 14 h light regimen to initiate egg laying.
Monochromatic
light
influence on sexual maturity, production performance and egg
shell quality of laying hens.
31
Photoperiod 336d egg
production
Egg
weight(g)
Shell
deformation(μg)
Rearing Laying
8h 14h 271a 58.4b 26.5a
14h 14h 256b 60a 25.4b
Effect of rearing day length on subsequent layer performance
Summers and Leeson (1983)
Effect of monochromatic light on sexual maturity, production
performance and egg quality of laying hens
Kim Min et al. (2012)
Variable
Treatments
Incandescent
light (control)
White light Blue light Red light
Egg production (%)
18–30 weeks 76.18 ± 27.22ab
77.41 ± 28.10ab 64.97 ± 33.48b 79.23 ± 21.25a
31–40 weeks 88.43 ± 6.26b 93.60 ± 3.81a 89.10 ± 5.65b 93.90 ± 4.37a
41–50 weeks 83.10 ± 7.13b 86.63 ± 6.96ab 86.20 ± 6.00ab 89.70 ± 6.14a
51–60 weeks 87.80 ± 7.42b 88.73 ± 6.98ab 88.20 ± 5.71b 91.63 ± 3.97a
18–60 weeks 83.34 ± 16.71bc 85.95 ± 17.25ab 80.92 ± 21.62c 87.96 ± 13.63a
Egg weight (g)
18–30 weeks 56.12 ± 10.32 56.13 ± 4.98 51.16 ± 18.17 56.24 ± 5.21
31–40 weeks 60.89 ± 2.47 60.09 ± 1.32 60.51 ± 1.35 60.20 ± 2.23
41–50 weeks 60.94 ± 1.73ab 59.86 ± 3.64b 61.70 ± 2.83a 59.98 ± 1.93b
51–60 weeks 61.12 ± 1.40 61.96 ± 3.86 61.89 ± 2.85 61.20 ± 1.85
18–60 weeks 59.51 ± 6.26 59.27 ± 4.37 58.28 ± 11.16 59.18 ± 3.85
Age at 1st egg and 50% egg of laying hens (Days)
1st egg 125 ± 1.86b 122 ± 1.03bc 131 ± 3.14a 120 ± 1.55c
50% egg 134 ± 2.58b 133 ± 3.22bc 144 ± 2.73a 130 ± 1.37c
32
Effect of age at photo stimulation on sexual maturation and egg-
laying performance of layer breeders
Shi et al.(2020)
N=192 WL breeder hens of 14 wk of similar BW (average ± 10%) ,4T(PS16, PS18, PS20, and
PS22),2R(24/R). 1st week of PS: Intensity 80 lux, 2nd week of PS: change in lighting
regimen to 14L:10D by increasing day length by 4h and then by 1h each 3rd and 4th week
PS at 18 wk is appreciated for the WL breeder hens.
Item 16 18 20 22 SEM P-value
BW at sexual maturity, g 1372.39 1381.40 1438.77 1454.57 16.15 0.18
Age at 5% egg production, D 134.00 132.50 141.00 141.00 1.85 0.21
Age at 50% egg production, D 149.50 148.50 154.50 154.00 1.32 0.28
Peak of laying, % 91.96a 93.37a 88.91a 78.81b 2.26 0.02
Ovary weight, % 3.03 2.92 2.98 2.86 0.05 0.96
Egg number1, n 161.18a 162.64a 145.53a 133.54a 4.80 0.02
33
Housing Management
Du et al. (2022)
WELFARE
Housing
Type
Lighting
Management
Humidity
Temperature
Ventilation
Vaccination
Biosecurity
34
Feeding 22-20-19% CP during rearing period have highest HDEP.
5% inclusion of palm oil in grower diet have higher HHEP and EW.
Linoleic acid play an important role in maximizing the egg size.
Best prelay diet(1800kcal/kg ME, 18% CP and 2% Ca)with BIS layer diet is the best
combination for improving EN, HDEP, FCDE, EFPR and PEI.
The light weight (1.05-1.3 kg) hens had the higher HDEP.
Feed restriction delayed sexual maturity, improve EW, increase EP, improve
uniformity into flock.
PS at 18 woa and Red Monochromatic light influence early SM, increased EP and
EW in laying hen
35
Conclusions
36
Future Prospectus
Genetic, Nutritional, Housing managemental
intervention of pullet for pullet development need
to emphasis for maximum exploitation of
commercial layer.
37
THANK YOU…!

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advanced Newcatle vet.pptx

  • 1. Nitin Dhore V/16/258 DEPARTMENT OF POULTRY SCIENCE Advances in Pullet Management KRANTISINH NANA PATIL COLLEGE OF VETERINARY SCIENCE SHIRWAL, DIST. SATARA (MS) MASTER CREDIT SEMINAR PSC: 691 (0+1) ON MAHARASHTRA ANIMAL & FISHERY SCIENCES UNIVERSITY, NAGPUR
  • 2. Introduction Pullet Development Nutritional Intervention Managemental Intervention Genetic Intervention Conclusions Future Prospectus
  • 3. Statistics (Basic Animal Husbandry and Fisheries Statistic 2022) 2.43% of the World poultry population Present in India The Indian poultry industry: ranking 3rd in egg production Growth rate 8 to 10% Production of 129.60 billion eggs in 2021-22 Total population: 851.81 million (India) Maharashtra has 74.3 million (Ranked 5th )
  • 4. Pullet? Karcher et al. (2018) • Female that have not laid an egg are referred to as pullets. The term pullet is used until approximately 18 weeks of age, at the point egg production begins. • The pullet developed according to a well-orchestrated sequence of physiologic events. • Nutrition is the key to optimal genetic performance. • Interrupted growth during this developmental phase: results in lacks of body reserves and organ function fail to sustain high production.
  • 5. Fast intestine development Fully developed intestine & immune system 6 12 Fastest skeletal growth 90-100g BW/wk 95% of skeleton development Reaches to 95% of adult size but only 75% of mature weight Muscle growth, medullary bone & reproductive tract development will continue much of WG Full mature BW achieved Complete transition of skeletal bone to building medullary bone. Estrogen stimulates oviduct development 2-3 wk To aid medullary bone growth before 1st egg , it is recommended to introduce more Ca through PRELAY DIET Pullet Development 18 16 Lonkar et al, (2022)
  • 6. 6 Skeletal problem. Low body weight. Variation in flock. Cage fatigue. Low body reserve. Poor feather Cover. Poor performance. Delayed sexual maturity. Uniformity flock. Correct body reserve. Medullary calcium reserve. Correct age at sexual maturity. Good body condition. Skeletal development. Good Layer Improve Body Reserve Sustain egg production. Better egg quality. Ability to lay extended laying cycle. Good welfare. Increase per capita availabilty. Extended laying cycle result into pullet cost dilution Fuel Poor Pullet Good Pullet
  • 7. 7 Nutrition Dietary ME & CP Dietary fat Prelay Restricted feeding Management Lighting Vaccination Stocking density Biosecurity Temperature & Humidity Genetics Intensive selection of trait Breeding programme Fuel
  • 8. Importance of Pullet Nutrition Body weight Body composition Medullary bone development Uniformity Feather cover • Pullet feed contributes indirectly to egg production by influencing sexual maturity, live weight and body composition at the onset of lay. • Optimum feeding management practices followed during the rearing period in pullets have effects on body weight, uniformity, egg production, egg weight, egg quality, and hatching properties (Sarica et al., 2009). • Growing period of a laying hen is the most critical time in a hen’s life and the mistakes made during this period are difficult to rectify. 8 Pullet Nutrition
  • 9. Importance of Pullet Nutrition • Elevated Ca: P ratio in pullet and prelay diets decreased femur-breaking strength at the end of layer phase without affecting pullet BWG and pullet feed efficiency in the cage system. • Prelay diet containing 2700 kcal ME/kg and 18% CP for pullets before sexual maturity to improve body composition and medullary bone development. • Insufficient dietary Ca during rearing period has an adverse effect on eggshell quality and bone strength. • Protein intake during pullet-to-breeder transition period: improve early EW and EP by increasing CP levels of in prelay diets. 9 Joseph et al. (2000) Whitehead(2004) Sujatha et al.(2013) Fosnaught(2009)
  • 10. Effect of Dietary CP and ME Levels During Growing Period on Early Egg Production Feeding the higher protein levels (P2 and P3) had the highest hen-day egg production and egg mass. The feeding of the pullets with P3E3 diet during the growing period will give best hen-day egg production. Babiker et al. (2010) N=270,9T,3R(10/R) Experimental period was divided into four phases. In all phases of growing period, control levels of protein (P1) and energy (E1) were set according to NRC (1994) and the other levels were higher, for protein in 2% steps (P2 and P3) and for energy by 100 kcal of ME/kg steps (E2 and E3). 19th wk onward commercial layer diet upto end of trial. Hen Day Egg Production (%) Energy Hen Day Egg Production(%) Protein Effect ** Energy Effect NS P1 18-16-15 50.1b E1(3000-3000-3050) 54.2 P2 20-18-17 56.1a E2(3100-3100-3150) 54.5 P3 22-20-19 58.8a E3(3200-3200-3250) 56.3 Age effect ** 22 16.3c 24 51.5b 28 70.0a 32 71.3a 36 65.8a 10
  • 11. Growth and egg production of layer pullets can be affected by the method of supplying energy and protein sources N=320, 9woa brown egg laying strain pullet (ISA),4T control (balanced diet); E60:P40,E40:P60,EPFC (free choice between ES and PS in separate troughs) 4R(20/R).11 h of light at the initiation of the experiment and reached 14.5 h of light at 21 weeks of age. Egg laying start lighting programme was gradually increased from 14.5 to 16 h of light upto 33 wk of age. Ocak et al.(2009) • Feeding PS during 60% of the daily photoperiod increased egg production by improving the feed efficiency compared with other treatments. Parameter Controla E60:P40 E40:P60 EPFC SEM Hen-day egg production(22-33wk) HDEP% 87.3ab 84.3b 88.4a 86.0ab 0.59 Egg weight (g) 63.9 63.9 63.5 63.0 0.22 11
  • 12. Effect of Dietary fat on pullet developement The most important factors determining egg weight is body weight of hen at the age of sexual maturity. Summers and Leeson (1983) Increasing dietary energy or dietary fat significantly increases early egg weight due to increased in Yolk weight. Wu et al. (2005) Birds receiving 4% corn oil (2.08% linoleic acid) produce numerically larger eggs, best feed efficiency and gain slightly more BW as compared to birds receiving 0% corn oil. Najib and Sullivan (1986) 12
  • 13. Palm Oil and Animal Fats for Increasing Dietary Energy in Rearing Pullets Isika et al. (2006) N=300, 5 T, 3R(20PULLET/R),(NERA STRAIN PULLET),Birds fed grower diet containing 0%(control),2.5 and 5 % palm oil, 2.5 and 5% broiler fat. From 22nd woa commercial layer diet was fed. 13 Ingredients Palm Oil Broiler Fat PO/BF inclusion (%) 0.0 2.5 5.0 2.5 5.0 a. Ages: Week At 1st egg 18b 18b 19a 19a 18b ii. Hen-house egg production 67.8c 70.5ab 73.3a 67.3c 71.2a d. Egg qualities I. Size (g/egg) 50.4b 51.3b 57.1a 54.0b 53.1b ii. Shell thickness(mm) 0.34 0.33 0.34 0.333 0.32 5% inclusion of palm oil and broiler fat higher hen housed egg production and had higher egg weight.
  • 14. Egg Size of Saudi Local Layers as Affected by Dietary Fat Level Huthail Najib and Yousif Al-Yousif (2014) Linoleic acid play an important role in maximizing the egg size of the local birds by 4% corn oil. It is a good way of improving the egg size of the Saudi local brown birds during the onset and first part of the laying year. 14 N=120, 2*3 FACTORIAL DESIGN (black, light (700-900 gm) & Brown, medium (900-1080 gm).* Corn oil, 0, 2 and 4%, 6T(20/T), 4R(5/R) at the age of 22 weeks. Source of variation HD EM EW Levels NS NS ** 0 48.26a 22.52a 46.47a 2 48.02a 22.82a 47.29b 4 49.77a 24.68a 48.92c p = 0.7535 0.1734 <0.0001
  • 15. Adequate reserve in medullary bones at beginning of the production cycle is crucial in reducing cage fatigue, in maintaining a good EP and formation of eggs with a good eggshell quality Moderate Ca levels (1.5-2.75%) fed during the prelay period allows for the buildup of the medullary bone reserves of Ca without adversely affecting kidney function and may have an effect on shell quality in subsequent laying cycle. A prelay diet is introduce before the commencement of EP is an extremely important period in the life of a high genetic potential layer. Good body reserve (fat and protein), positive energy balance and Ca reserves in the medullary bone at the onset of production is essential for sustained EP and egg size (Miles & Jacqueline, 2008) (Williams et al., 2000) (Sujatha & Rajini, 2015) Prelay Diet
  • 16. Prelay feeding changes the pullet body composition N=360,5 pre-lay diet, 6R(12/5),fed during 15woa to sexual maturity in experiment 1. In experiment 2. Best two nutrient compositions with respect to body composition of pullets from Exp.1 and fed with 1,1.5, and 2% Ca. Sujatha et al.(2013) Treatments (ME/CP) Crude Protein % Fat % 2500/16 21.54b±0.25 7.31b±0.09 2700/16 21.34b±0.15 11.29a±0.35 2700/18 24.38a±0.48 11.44a±0.14 2700/16+10% L+M 21.34b±0.15 11.29a±0.35 2700/16+10% L+M + 2% oil 21.54b±0.31 13.32a±0.05 • It could be concluded that pre-lay diet containing 2700 kcal/kg of dietary energy, 18 per cent CP and 2 per cent calcium is advisable for pullets before sexual maturity 16
  • 17. Nutrient density in Prelay diet affect the laying performance Sujatha et al,(2014) Pre-lay treatments Control-2500/16* 2700/16% 2700/18* 2700/16 (10% L&M)* 2700/16 (10% L&M +2% oil)* Hen day egg production (eggs hen/d) Age in Weeks Standard layer feed 20-23NS 0.434C (± 4.46) 0.465C (± 4.89) 0.519B (± 5.37) 0.466C (± 3.64) 0.469C (± 4.99) 24-27* 0.725B,c (± 0.99) 0.793B,b (± 1.81) 0.904A,a (± 1.04) 0.789B,b (± 1.86) 0.734B,c (± 1.80) 28-31* 0.788A,c (± 0.50) 0.851B,C,b (± 1.76) 0.946A,a (± 135) 0867A,b (± 1.85) 0841A,b (± 1.71) 32-35* 0.795A,B,c (± 2.83) 0.935A,a (± 1.43) 0.912A,a (± 1.36) 0.879A,a (± 1.67) 0.877A,a (± 1.81) 36-39* 0.837A,c (± 1.56) 0.942A,a (±1.81) 0.957A,a (± 0.702) 0.867A,b,c (± 1.53) 0.888A,b (± 1.84) 40-43* 0.765A,B,c (± 1.16) 0.869A,a (± 1.64) 0.886A,a (± 0.796) 0.747B,b (± 1.51) 0.827A,b (± 1.54) Overall mean* 0.7245c (± 1.40) 0.809b (± 1.6) 0.853a (± 1.52) 0.769b (± 1.40) 0.773b (± 1.52) Egg weight (gm) Age in Weeks Standard layer feed 20-23NS 44.86D (± 0.52) 45.55C (± 067) 46.20D (± 0.74) 45.86C (± 0.81) 46.50D (±0.79) 24-27* 50.42C,c (± 0.23) 51.25B,a,b (± 021) 51.34C,a,b (±024) 50.59B,b,c (± 0.36) 5.30C,a (±0.24) 28-31* 54.11B,c (± 0.22) 55.23A,a,b (± 0.20) 56.12B,a (± 0.41) 54.86A,b,c (± 0.38) 55.41B,a,b (±0.28) 32-35* 55.50A,b (± 0.23) 56.99A,a,b (± 1.09) 57.58A,a (± 039) 55.72A,b (±0.29) 56.94A,a,b (±0.31) 36-39* 55.02A,c (± 0.19) 56.58A,b (± 0.31) 57.50A,a (± 0.35) 55.17A,c (±0.18) 56.20A,B,b (±021) 40-43* 54.03B,b (± 0.12) 56.43A,a (± 0.49) 57.22A,B,a (± 0.44) 55.01A,b (±0.37) 56.17A,B,a (±0.25) Overall mean* 52.34c (± 0.31) 53.67a,b (± 0.39) 54.33a (± 0.37) 52.87b,c (±0.32) 53.79a,b (±0.33) 17
  • 18. Effects of dietary calcium and phosphorus level on egg shell in brown egg-type pullets from 0 to 32 weeks of age N=1380,6T,10R(23PULLET/R), Dietary Ca and P high(7.5-6.7-7.3:3.6-3.2-3.0) and low(5.8-4.7-5.0:3.2-2.6-2.4)Ca-P) from respectively 0 to 6, 6 to 11,and 11 to 16 wk of age). WEEKS 22nd 25th 32nd EFFECT Egg shell thicknesss (mm) Egg shell thicknesss (mm) Egg shell thicknesss (mm) HIGH (Ca:P) (7.5-6.7-7.3:3.6-3.2-3.0) 0.401 0.406 0.391b LOW (Ca:P) (5.8-4.7-5.0:3.2-2.6-2.4) 0.397 0.402 0.402a Egg shell thickness at 22, 25, and 32 wk of age, fed diets during rearing (0 to 16 wk of age) and high or low Ca and P level. • Low dietary Ca-P during rearing had no clear effect on pullet development and egg production, but some improvements on egg shell quality were identified at 32 wk of age. Dijkslag et al.(2021) 18
  • 19. Targeting the Pullet Body Weight for efficient layer performance 19 Most important factors determining egg weight is body weight of hen at the age of sexual maturity. Oke et al. (2004) Hens with low or average initial body weight had higher EP and EW than the lighter hens. Perez-Bonilla et al. (2012) BW and BC play an important role in sexual maturation and egg production in laying hens. Hanlon et al. (2021)
  • 20. N=96 shaver brown 6th wk of lay, 4T(24/T) (T1= 0.9-1.2kg, T2= 1.3-1.4kg, T3=1.5-1.6kg and T4= 1.7-2.0kg). 4R(6/R). Edeh et al.(2020) The effect of bodyweight variation on laying performances of Shaver brown hen in humid tropical environment Parameters T1 T2 T3 T4 Sig Body Weight(kg) 1.05 1.35 1.55 1.85 NS Average Daily Feed Intake, g 73.50±0.29ab 75.5±0.65ab 69.25±4.19b 78.50±1.32a * Egg Weight(g) 61.89±0.62b 63.22±0.64ab 64.62±1.81a 64.65±1.06a * Hen Day egg Production, % 73.97±1.07a 74.30±0.81a 70.35±0.35b 69.65±0.67b 20 The light weight hens had the highest yield and less cost of feeding which is more profitable to farmers.
  • 21. BODY FAT Both low and high body fat contents could have a negative effect on the productivity of hens. 21 Hocking et al. (2002) Body fat threshold between 10% to 15% is required to initiate sexual maturation. Bahry et al. (2023)
  • 22. N=45 TETRA SL laying hens,3T.Experiment was started at 20 weeks of age. • The high initial body fat content of the hens resulted in the lowest egg production intensity at all examination days. Milisits et al.(2016) Effect of Low, medium and high body fat composition of pullet on egg production 22 Egg production(%) Initial body fat content Level of significance (P) IBF S.E Wks Low 22.9% Medium 24.9% High 27.2% 32 94.9 96.2 98.3 0.400 0.8 52 96.2b 90.2a 87.1a 0.010 1.3 72 80.0b 76.1ab 73.2a 0.039 1.1 Egg weight(g) 32 63.4 62.5 61.5 0.526 0.7 52 65.9 62.0 64.2 0.112 0.7 72 66.5 65.9 64.6 0.755 0.9
  • 23. Effect of starting body fat content of laying hens on the egg production during the first egg laying period • The starting body fat content of laying hens has significant effects on egg production Milisits et al. (2015) TETRA SL Starting body fat content Low 23.1% Average25..6% High28..4% Hen day egg production TETRA SL 332b±13 331b±13 318a±20 Effect of starting body fat content of laying hens on the number of total eggs laid by the two genotypes (mean±SE) 23 N=120,(60 TETRA BLANCA white egg layers and 60 TETRA SL brown egg layers),Pullets based on their body fat content at 16 weeks of age.
  • 24. Feed Restriction during Grower phase. • Manipulate growth and metabolism to get desirable body weight during laying which in return helps to delay sexual maturity and increases production performance of the birds without affecting their welfare or health. • Increase the overall egg production, settable eggs, and average egg weight, and enhance fertility, hatchability, and egg quality in broiler breeders. 24 Tesfaye et al. (2009) Avila et al. (2023)
  • 25. Effects of feed restriction on production performance of Rhode Island red pullets Tesfaye et al. (2009) Feed restrictions by skip a-day feeding system at middle age of development (T3) resulted in higher egg weight and this can be considered as the optimum age for pullet rearing. N=240(48.92 ± 1.53 g),4T, 3R(20/R), 4 feeding treatments [T1 (Unrestricted, Control), T2 (Restricted at 7, 14, 21 and 28 days of age), T3 (Restricted at 35, 42, 49 and 56 days of age) and T4 (Restricted at 63, 70, 77 and 84 days of age] using (CRD).Commercial starter layer diet (0-6)weeks and commercial grower layer diet (7-22 woa). Parameters Treatments T1 T2 T3 T4 SE P- value Age at onset of egg laying (days) 140.80 ± 0.80bc 140.17 ± 2.50c 147.00 ± 2.20a 143.83 ± 2.30b 0.96 * Body weight (g) at first egg 1305.00 ± 13.20b 1374.30 ± 23.20ab 1352.00 ± 23.50ab 1429.23 ± 44.50a 15.18 ** Egg weight (g) 42.68 ± 0.72b 42.43 ± 1.91b 47.9.00 ± 0.87 a 41.88 ± 0.77b 0.79 ** Shell thickness (mm) 0.26 ± 0.01ab 0.25 ± 0.00b 0.26 ± 0.01ab 0.29 ± 0.01a 0.01 ** 25
  • 26. Effects of energy restriction during growing phase on the productive performance of Hyline Brown laying hens aged 6–72 wks Lu et al.(2023) Weeks Contro l 2,850 Energy-restricted feeding (6-17 wk) SEM P-value Energy restriction level Linear Quadrat ic 2,565 2,280 19−20 wk 2.41a 0.45b 0.00c 0.552 0.046 0.038 0.502 21−24 wk 37.94a 31.12a 17.51b 2.540 <0.001 <0.001 0.328 25−28 wk 93.45 94.40 90.94 0.761 0.160 0.173 0.168 29−32 wk 93.34 93.28 96.41 0.692 0.102 0.066 0.254 33−36 wk 93.77b 93.87b 98.35a 0.695 0.002 0.002 0.054 37−40 wk 93.60b 95.61ab 97.14a 0.594 0.039 0.012 0.831 41−44 wk 91.01b 95.45a 96.70a 0.851 0.007 0.003 0.266 45−48 wk 90.57b 93.29ab 94.54a 0.643 0.024 0.008 0.526 49−52 wk 90.67b 92.42ab 94.18a 0.639 0.072 0.024 0.994 53−56 wk 86.88b 90.66a 91.27a 0.727 0.017 0.008 0.224 57−60 wk 82.86b 88.86a 89.78a 1.077 0.008 0.004 0.170 61−64 wk 82.30b 90.10a 90.28a 1.208 0.002 0.002 0.053 65−68 wk 80.00b 90.31a 90.18a 1.408 <0.001 <0.001 0.010 69−72 wk 75.97b 86.72a 86.53a 1.578 0.001 0.001 0.029 26 a-c Means without common superscripts within a row differ significantly (P < 0.05).
  • 27. 27 Pullet Uniformity Standard for Pullet Management 85% ± 10% Excellent Uniformity 82-85 ± 10% Very Good Uniformity 70% ± 10% Unsatisfactory
  • 28. The Effects of Dietary Whole Rice Hull as Insoluble Fiber on the Flock Uniformity of Pullets Laying Hens N=1500 N Brown Nick pullet chicks of 4 woa (uniform weight) divided into 3T,5R(100/R) They were provided with ad libitum access to water and fed the basal grower diets (CP, 19%; ME, 2, 950 kcal kg−1 ) containing 0 (control), 3 and 6% WRH. Incharoen et al.(2013) A greater enhancement of growth rate and percentage of flock uniformity were observed in pullet chicks fed both dietary WRH levels, compared to birds in the control group Parameters Control 3% WRH 6% WRH Initial body weight, g (4woa) 526±1.38 521±2.51 527±1.41 Final body weight, g () 1107±4.17b 1129±2.81a 1130±2.51a Body weight gain, g 580±4.93b 608±5.29a 602±2.65a Feed intake, g 2063±2.46c 2132±10.50b 2205±16.53a Feed conversion ratio (FCR) 3.56±0.03a 3.50±0.04a 3.66±0.02b Flock uniformity within ± 10% of each pen mean (%) 85.0±1.70 87.4±1.84 86.8±1.93 28
  • 29. 29 Light Influences physiological responses, behavior, growth and development, and production performance in poultry. Blue and green light Improve growth, help to calm down, promote immune performance (Zhang et al., 2014), and improve egg quality (Er et al., 2007). Red light Increase the levels of reproductive hormones, promote the development of sexual organs, influence the age of sexual maturity (SM) of pullets, improve production performance, and reduces the incidence of feather pecking and cannibalism. Blue–Green light Stimulated growth of chickens Orange–Red Stimulated reproduction development (Rozenboim et al., 1999). Widely applied in poultry production likely do not meet the requirements for the welfare and health of layer chickens, especially for the brooding and rearing periods (0–20 wk of age), White LED and monochrome LED lights Advances in Light Management in Pullets (Borille et al., 2015) (Liu et al., 2017) (Hassan et al., 2013)
  • 30. 30 Wei et al. (2020) Yellow –Orange phase 2 (14–20 wk of age) Increased the BMD concentrations, promoted sexual organ (oviduct and ovary) development, advanced the age of sexual maturation, and improved the production uniformity of layer chickens Blue –Green phase (0–13 wk of age) Increased the serum Ig concentrations and serum GLU concentration levels of layer chickens Wei et al. (2020) Typically, pullets are reared under an 8–10 h photoperiod and photostimulated at 16–22 weeks of age with a 14 h light regimen to initiate egg laying. Monochromatic light influence on sexual maturity, production performance and egg shell quality of laying hens.
  • 31. 31 Photoperiod 336d egg production Egg weight(g) Shell deformation(μg) Rearing Laying 8h 14h 271a 58.4b 26.5a 14h 14h 256b 60a 25.4b Effect of rearing day length on subsequent layer performance Summers and Leeson (1983)
  • 32. Effect of monochromatic light on sexual maturity, production performance and egg quality of laying hens Kim Min et al. (2012) Variable Treatments Incandescent light (control) White light Blue light Red light Egg production (%) 18–30 weeks 76.18 ± 27.22ab 77.41 ± 28.10ab 64.97 ± 33.48b 79.23 ± 21.25a 31–40 weeks 88.43 ± 6.26b 93.60 ± 3.81a 89.10 ± 5.65b 93.90 ± 4.37a 41–50 weeks 83.10 ± 7.13b 86.63 ± 6.96ab 86.20 ± 6.00ab 89.70 ± 6.14a 51–60 weeks 87.80 ± 7.42b 88.73 ± 6.98ab 88.20 ± 5.71b 91.63 ± 3.97a 18–60 weeks 83.34 ± 16.71bc 85.95 ± 17.25ab 80.92 ± 21.62c 87.96 ± 13.63a Egg weight (g) 18–30 weeks 56.12 ± 10.32 56.13 ± 4.98 51.16 ± 18.17 56.24 ± 5.21 31–40 weeks 60.89 ± 2.47 60.09 ± 1.32 60.51 ± 1.35 60.20 ± 2.23 41–50 weeks 60.94 ± 1.73ab 59.86 ± 3.64b 61.70 ± 2.83a 59.98 ± 1.93b 51–60 weeks 61.12 ± 1.40 61.96 ± 3.86 61.89 ± 2.85 61.20 ± 1.85 18–60 weeks 59.51 ± 6.26 59.27 ± 4.37 58.28 ± 11.16 59.18 ± 3.85 Age at 1st egg and 50% egg of laying hens (Days) 1st egg 125 ± 1.86b 122 ± 1.03bc 131 ± 3.14a 120 ± 1.55c 50% egg 134 ± 2.58b 133 ± 3.22bc 144 ± 2.73a 130 ± 1.37c 32
  • 33. Effect of age at photo stimulation on sexual maturation and egg- laying performance of layer breeders Shi et al.(2020) N=192 WL breeder hens of 14 wk of similar BW (average ± 10%) ,4T(PS16, PS18, PS20, and PS22),2R(24/R). 1st week of PS: Intensity 80 lux, 2nd week of PS: change in lighting regimen to 14L:10D by increasing day length by 4h and then by 1h each 3rd and 4th week PS at 18 wk is appreciated for the WL breeder hens. Item 16 18 20 22 SEM P-value BW at sexual maturity, g 1372.39 1381.40 1438.77 1454.57 16.15 0.18 Age at 5% egg production, D 134.00 132.50 141.00 141.00 1.85 0.21 Age at 50% egg production, D 149.50 148.50 154.50 154.00 1.32 0.28 Peak of laying, % 91.96a 93.37a 88.91a 78.81b 2.26 0.02 Ovary weight, % 3.03 2.92 2.98 2.86 0.05 0.96 Egg number1, n 161.18a 162.64a 145.53a 133.54a 4.80 0.02 33
  • 34. Housing Management Du et al. (2022) WELFARE Housing Type Lighting Management Humidity Temperature Ventilation Vaccination Biosecurity 34
  • 35. Feeding 22-20-19% CP during rearing period have highest HDEP. 5% inclusion of palm oil in grower diet have higher HHEP and EW. Linoleic acid play an important role in maximizing the egg size. Best prelay diet(1800kcal/kg ME, 18% CP and 2% Ca)with BIS layer diet is the best combination for improving EN, HDEP, FCDE, EFPR and PEI. The light weight (1.05-1.3 kg) hens had the higher HDEP. Feed restriction delayed sexual maturity, improve EW, increase EP, improve uniformity into flock. PS at 18 woa and Red Monochromatic light influence early SM, increased EP and EW in laying hen 35 Conclusions
  • 36. 36 Future Prospectus Genetic, Nutritional, Housing managemental intervention of pullet for pullet development need to emphasis for maximum exploitation of commercial layer.

Editor's Notes

  1. This is the outline of mine master credit seminar
  2. Coming to statistics india rank 3rd in egg production with growth rate of 8-10%,with production of 129.6 billion eggs,with per capita availability of 95 egg per person which is below the icmr recommendation of 180eggs per year.
  3. Week 0-6: The organs of the digestive tract and immune system are developing. Stress at this age can have a lifelong impact on the digestion of feed and the absorption of nutrients. Week 6-12: This is the period of rapid growth and when the pullet is building most of her adult structural components (muscle, bones, and feathers). Week 12-18: Growth is beginning to slow while the reproductive tract matures and prepares for egg production. The skeleton is 95% developed by the end of the 13th week of life, and at this time, the skeleton size will not increase. Muscles continue to develop and fat cells begin to proliferate in preparation for sexual maturity. You should avoid excessive body weight gain at this period because it can result in an excessive fat pad. Low bodyweight and stress can delay the onset of egg production.
  4. What are the intervention to create good quality pullet. Objective of breeding programme include maximum number of saleable egg.optimal internal and external egg quality. Low feed cost per kg egg mass, and low mortality and adaptability to environment. Select such a strain which give better egg production during laying period with better egg quality. And select such trait that are essential for egg egg production.
  5. So we will see the nutrition intervention
  6. Phase one (starter) from 0-6 wk (P1 = 18, P2 = 20 and P3 = 22% CP) (E1 = 3000, E2 = 3100 and E3 = 3200 kcal of ME/kg of diet), phase two (grower) from 7-12 wk (P1 = 16%, P2 = 18% and P3 = 20%) (E1 = 3000, E2 = 3100 and E3 = 3200 kcal of ME/kg of diet), phase three (developer) from 13-18 wk and phase four (production) (P1 = 15%, P2 = 17% and P3 = 19%) (E1 = 3050, E2 = 3150 and E3 = 3250 kcal of ME/kg of diet). The pullets were exposed to decreasing lighting period by two hours weekly from 23-12 h day length up to the sixth week of age. The lighting period was kept constant until 18 wk of age. Thereafter the day length was increased by two hours of artificial light weekly until it reached 16 hours per day at 20 wk of age. Egg production recorded from 22-36wk of age. Result: feeding different protein regimen during rearing period
  7. The pullets were then individually weighed, placed in a deep pit layer house (ventilated both naturally and mechanically and illuminated both artificially and naturally through the windows) and fed commercial layer diets. The birds in treatment 4 were fed free choice between ES and PS in separate troughs throughout the daily photoperiod (EPFC). While birds in the E60:P40 group were fed ES during the morning and PS during the afternoon, those in the E40:P60 group were fed PS during the morning and ES during the afternoon. HDEP calculated from 22-33 wk of age.
  8. It is concluded that 2.5% inclusion level of palm oil in pullet diets: earlier sexual maturity. Both palm oil and broiler fat can be used for increasing dietary energy for pullets.
  9. Commercial starter and grower feed were given to the birds from day 1 to 20 weeks of age. Lighting hours were held constant at 10-11 h daily till the end of the period. Trial lasted for 28 wk.
  10. Transitional pullet feed and its significance at sexual maturity
  11. N=360,5T,12R(6/R) T1 BIS CONTROL(16%,2500kcal ME),T2(16%,2700kcal ME), T3(18%,2700kcal),T4(16%,2700kcal+ 10% methionine + lysine), T5(16%,2700kcal,2% oil, + 10% methionine + lysine ) It could be concluded that a pre-layer diet is required for better egg production and it would be ideal to provide a diet with a 200 kcal above that recommended by BIS and with 2% higher crude protein levels from 15 weeks to 5% egg production for birds reared under a tropical maritime climate
  12. 1,380 day old female Bovans Brown egg-type pullets. The experimental diets were fed during the rearing period (0 to 16 wk of age). Pullets were kept on a 18 h/d light program for the first wk of life. Then, the light period was decreased by 1 h/wk from the second wk until reaching 12 h/d in wk 6 and this was continued till the end of the rearing period. 3×2 factorial arrangement(feed form (crumbles (CWS), mash (MWS), both with inclusion of 3% finely ground wheat straw, or crumbles with inclusion of 3% oat hulls (COH), and dietary Ca and P (high and low Ca-P) from 0 to 16 wk of age). Effect of feeding strategy during rearing on laying performance and egg shell quality was studied from 19 to 32 wk of age. Effect of feeding strategy during rearing on laying performance and egg shell quality was studied from 19 to 32 wk of age.
  13. Body weight is a critical factor in the development of pullets before they start laying eggs. Achieving the appropriate body weight at the onset of lay is important for several reasons:Egg Production and Quality: The body weight of pullets before they start laying is closely linked to their future egg production and egg quality. Pullets that have reached an adequate body weight are more likely to start laying eggs at the right time and produce a higher number of eggs over their laying cycle. Proper body weight also contributes to the production of larger and healthier eggs. Uniformity of body weight in pullets and laying hens is an important management concern. To achieve early maturity and egg production it is very important to have correct body weight and uniformity in the growing period. Average body weights of white laying pullets at the 6th, 12th and 16th week were 430, 890 and 1130 g, respectively.
  14. Energy Reserves: Body fat serves as an energy reserve for pullets. As they grow and develop, they require energy for various physiological processes, including muscle development, bone growth, and the formation of reproductive organs. Body fat acts as a source of energy during periods when the pullet's dietary intake may not meet its energy requirements. Hormone Production: Puberty Onset: Egg Production: insufficient body reserve befor eonset of egg production delay sexual maturity or low egg or small eggs. Temperature Regulation: Nutrient Storage: Fat-soluble vitamins (such as vitamins A, D, E, and K) are stored in body fat. These vitamins are essential for various physiological functions, including bone health, immune function, and vision. Adequate body fat ensures a reservoir of these vital nutrients.
  15. Three groups of pullets were chosen for the investigation respectively with the highest (n=20), lowest (n=20) and average (n=20) body fat content. egg production and egg composition of the chosen animals were recorded at 32, 52 and 72 weeks of age
  16. Brings uniformity in flock. Reduce bw before sexual maturity. Produce better egg size before the start of lay. Offer liveability during egg production.
  17. STARTER LAYER DIET CP% AND ME (20.22% and 3144 ME kcal/kg ) GROWER LAYER DIET CP% AND ME (18.52% and 2883 ME kcal/kg) Additionally, feed restriction at latter ages (T4) could also be recommended due to heavier uterus weight and longer length in absolute term as these attributes indicate that this group of birds has good consistency for the subsequent egg production.
  18. Material method:-N=720,3T,6R(40/R),EP (6-17WK),CONTROL DIET(2,850 kcal/kg AMEn, fed ad libitum), 2,565 kcal/kg AMEn, 2,280 kcal/kg AMEn, and the daily amount of feed was restricted to the absolute quantity of the diet consumed by pullets in control group. At the age of 18–72 wks, all hens were fed the same basal diet ad libitum formulated to meet the National Research Council (1994) recommendations for laying hens. CONCLUSION:- Appropriate ER during growing phase delayed sexual maturity and sexual organ development in early-laying Hyline Brown pullets, improved uniformity of BW, age at first egg laying, and individual egg number, and increased egg number per hen, laying rate, average egg mass, and number of settable eggs from 18 to 72 wks of age.
  19. What is pullet uniformity? The percentage of flock that fall within a range of the average flock bodyweight is a pullet uniformity. The birds fall within range of 1230 more or less of 10% considered as excellent flock. Advantage Easier feeding management. All birds comes to sexual maturity at atime Uniform egg size. Higher hen housed egg production.
  20. At 4 weeks of age, a total of 1,500 H and N Brown Nickpullet chicks with a similar uniform weight was randomly separated into 3 dietary groups (500 birds group−1 ), each with 5 replicates of 100 birds. They were provided with ad libitum access to water and fed the basal grower diets (CP, 19%; ME, 2, 950 kcal kg−1 ) containing 0 (control), 3 and 6% WRH. All birds were housed in pens of the same size (3×5 m) in a deep litter material with a rice hull floor. Body weight and feed intake were recorded until 8 weeks of age; Feed Conversion Ratio (FCR) and flock uniformity were also calculated.
  21. Even though an abrupt photostimulation does promote an earlier onset of maturity and lay, resulting in an increase in the total number of eggs produced during a cycle, photostimulation of laying hens is typically spread over a longer period by increasing the photoperiod in 15 or 30 min weekly increments to promote optimum egg weight.
  22. N=144, 14 WOA HYLINE BROWN CHICKEN REARED IN CAGES,4T(INCANDESCENT(C),WHITE LIGHT,BLUE,RED LIGHT USING LED) 3R(12/R). 20 LUX INTENSITY DURING TREATMENT. Monochromatic light has an influence early SM, increased EP and EW in laying hen, but the FCR was not improved under RL.
  23. From 14 wk to age at 5% egg production, birds were fed commercial corn- and soybean-based diet with 16.00% crude protein (CP), 11.70 MJ/kg metabolizable energy (ME), and 1.00% calcium. From age at 5% egg production to 51 wk, birds were fed 16.50% CP, 11.50 MJ/kg ME, and 3.50% calcium.
  24. Management practices of pullets can enhance their growth as well as their physical and mental condition,indirectly improve the welfare of bird. Pullet are reared in cage free system and in cage system. Proper space and during proper stocking density during rearing period improved the performance. More Stocking density increase the chances of pecking and cannibalism. Proper housing and stocking density is important. Light is an important environmental factor which could influence the behavior, growth, productivity, and welfare of poultry via three characteristics based on the natural photoperiod: duration, intensity, and color/wavelength. Proper lighting schedule, with proper light intensity is necessary for pullet development. Maintain temperature within the range of 18-25degree celcius, humidity 40% during growing and laying period and proper ventilation should be available in the farm as 4 cubic meter/kg bw/hr. Follow the scheduled vaccination as per the strain and place, as a preventive measure. Strictly follow biosecurity measure in the farm.