During the last decade evidence has mounted that cell mechanics play an important role in cancer development as well as in many other medical conditions. Therefore investigating these phenomena is of strong interest among physiologists and pharmacologists alike. Our company provides researchers in academia and industry with a platform technology that allows visualizing and analysing forces exerted by single-cells. Since cell mechanic properties are excellent biomarkers we developed a mechanosensitive assay to determine the metastatic potential of bone cancer cells.
4. Different Assay Options
30. May 2015
Migration
Images fixed cell Video footage
Live cell imaging
Video footage
Live cell imaging
Individual
mechano profiles
Twitching
5. Cell Migration
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24h observation video of human
bone cancer cells migrating - 1200x faster
25 m
6. Another Live-cell Assay
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Migration
Images fixed cell Video footage
Live cell imaging
Video footage
Live cell imaging
Individual
mechano profiles
Twitching
7. Twitching Assays
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Human induced pluripotent stem cells – 3.3x faster
Spontaneously twitching
8. Yet Another Assay Option
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Migration
Images fixed cell Video footage
Live cell imaging
Video footage
Live cell imaging
Individual
mechano profiles
Twitching
9. Mechanobiological Differences in
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0
5
10
15
20
25
30
35
40
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19
Number of covered Microposts
HuO9
M132
0
1
2
3
4
5
6
HuO9 M132
Avg. Force/post [nN]
HuO9 = parental cell line – cells are stronger and more spread out (n=302)
M132 = metastatic cell line – cells are weaker and smaller (n=302)
Human Bone Cancer Cells
10. Research Targets
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HuO9 bone cancer cell
25 m
Typical effects on mechano profiles
Change in genotype by
◦ Knock-in, knock-out of genes
◦ Viral infection
Add of pharmaceutical active compounds
Change in extra cellular matrix composition
Environment stiffness or topography
11. Why Cell Mechanics? Why Microposts?
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Advantages
Single clinical tissue biopsy provides sufficient cells for a large number of
tests
Enables screening of a wide range of candidate molecules with patient
derived cells in early phases of the drug discovery/development
Novel level of information for researchers already using tissue-
mechanics testing methods (e.g. wire-myography)
Enables automated testing of mechanical cells responses (migration,
contraction)
Easy to integrate
12. Examples of Micropost Assays
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Test Medical Condition Properties of Interest
Wound healing Open wound Sealing process and
potential influences
Pharmacological
active compounds
Cancer development/
primary tumors
Cell size and cyto skeleton
condition
Mutiple sclerosis Re-myelinisation of
glia-cells
Effects of
mutations
Cardiotrophic myopathy/
DiabetisType II
Cell contraction (frequency
and amplitude)
Controlled cell
differentiation
Production of cells for
regenerative medicine
Constant influence of stem
cells for „maturing“
13. Product Status
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Sensor matrix
◦ Ø=6.4 m, h=18 m, gap = 6.6 m
◦ Material = silicone rubber ~ 50kPa
◦ Surface coating
Application protocol
Mechano-profiling software
Bone cancer cell assay
Happily returning customers
200 m
14. Mechanobiology – The Race is on!
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Comparison ofTechnology Development: Numbers from Web of Science
0
50
100
150
200
250
300
Number of publications
Year of publication
Gen AND sequen*
Substrate AND elasticity OR
stiffness AND cell
1990 1991 1992 1993 1994 1995 1996 1997 1998 1999
2004 2005 2006 2007 2008 2009 2010 2011 2012 2013
Search:
15. Implementation Step-by-Step
TwinSlide Multi-well Plate Cell Force Kit
Cancer models
Cardio – Vascular
Diseases models
Lung disease
(COPD, Asthma)
Basic
Research
Pharmaceutical
R&D
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Identification &
Validation
Qualification Standardisation
16. ETH Founders Team Entrepreneurs
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Dr Nils Goedecke – CEO & CSO
Dipl. Biophysics, Berlin DE
PhD Analytical Chemistry, London UK
Post-doc MIT – Cambridge, MA US
Sen. Researcher ETH-Zurich, CH
Sen. Researcher UZH Uniklinik Balgrist, CH
Ralf Streichan – CTO & CFO
Dipl. Ing. Microsystems Tech., Berlin DE
Researcher /Engineer ETH Zurich, CH
Process Engineer printed circuit board (PCB)
manufacturing for medical implants, CH
17. Thank you for your time and interest.
Please contact us for further information.
MicroDuits GmbH
Stampfenbachstr. 52
CH - 8006 Zuerich
info@microduits.com
www.MicroDuits.com
30. May 2015
Editor's Notes
Technology Introduction
Was? Zellverhalten auf Microsensoren gibt Aufschluss über Grundfunktionen der Zelle.