Radio broadcasts

1. Submitted To:
Dr.Rana Farhat Mehmood

2.  Haleema Shakoor
 Rafia Atta
 Warda Bibi
 Munaza Inayat

3.  The word chromatography is originated from
“Radio” and “chromatography”
 It is defined as the type of chromatography
in which radio labeled substances are
qualitatively or quantitatively determined by
measuring the radioactivity of the substance.

4.  Radio chromatography is based on paper or
thin chromatography though it can also be
used in ion exchange column or gas
chromatography as well as in
electrophoresis.
 It uses a radiotracer as cation or anion or
radio labeled organic compound where
separated cation anion or a compound is
identified on the basis of characteristics
radiation emitted by radio nuclide.

5. Chromatographic separations may be regarded as
falling into two classes;
1) Static
2) Dynamic
1)Static:
 Thin layer chromatography
 High Performance Liquid Chromatography
 Paper Chromatography and Electrophoresis fall
into this class.
These techniques give rise to a static
distribution of components whose radioactivity
may be subsequently determined.

6. 2) Dynamic :
 Gas Chromatography
 Liquid Chromatography
 Fall into this class.

7.  Chromatography involving radio labelled
materials has become increasingly important
in recent years ,both for the separation and
purification of radio labeled compounds and
for the analysis of mixtures from tracer .
 Radio chromatography has played an
important role in the discovery of some trans
plutonium elements by Glen T Seaborg.

8.  The identity of each elements 5f series was
established beyond by the sequence of their
appearance analogous to the sequence of the
corresponding 4f elements.
 There are many instances of the use radio
sssschromatography for analytical purpose.

9.  Estimating radiochemical impurities.
 Determination of impurities in radio labeled
compounds .
 Radioisotopes have been used to study the
movement of fertilizers.
 Clinical chemistry of nuclear medicine
 Radiopharmaceutical research and
development laboratory

10.  Drug metabolism studies .
 Soil leaching studies.

11.  Autoadiography
 Gamma camera
 Ionization chamber
 cut and count
 Chromatogram scanning
 Linear analyzer
 Electronic autoradiographys