in this presentation you will find an analysis of: dexmedetomidine alleviates myocardial ischemia/reperfusion-induced injury and ca2+ overload via the microrna-346-3p/camkiid axis article

1. MIGUEL ANTONIO GÓMEZ GIRALDO
THIRD SEMESTER OF MEDICINE
UPB

2. Introduction
myocardial ischemia
reperfusion
• Is the restoration of blood
supply to heart tissue
that is ischemic
• Death of cardiomyocytes
occurs in the first few
minutes of reperfusion
https://www.escardio.org/static-file/Escardio/Education/Courses/Basic%20science%20summer%20school/Revised_Pagliaro.pdf

3. MicroRNAs in the
treatment of
myocardial ischemia
• They are a class of small
non-coding RNAs linked to
cardiac disorders such as
myocardial infarction
• miRNAs determine the
fate of the heart by
regulating cardiac cell
death and regeneration
after MI

4. Objetive
EXAMINE THE EFFECT OF DEXMEDETOMIDINE TO ALLEVIATE
MYOCARDIAL ISCHEMIA/REPERFUSION-INDUCED INJURY AND
CA2+ OVERLOAD VIA THE MICRORNA-346-3P/CAMKIID AXIS

5. Methods
ENZYME-LINKED IMMUNOSORBENT ASSAY
(ELISA) FLOW CYTOMETRY

6. WESTERN BLOTTING (WB) REVERSE TRANSCRIPTION-QUANTITATIVE
POLYMERASE CHAIN REACTION (RT-QPCR)

7. Results
Western blotting (WB)
The analysis is done with 2
molecules that are in all the cells of
the body, the first is used to check
the results or evaluate the changes,
in this case B-actin. The other
molecule will be the one we want to
evaluate and study, for us it will be
the CaMKIId (Calcium/calmodulin
dependent protein kinase II delta)

8. Flow cytometry
In this case we want to
evaluate the apoptosis of
H/R cells, comparing the
results with the Blank cells
and some which DEX
(Dexmedetomidine) is
applied.
H2DCFDA
fluorescent
labeling
Using fluorescence we seek
to mark the intracellular
reactive oxygen species
(ROS) that appear in the
microRNA-346-3p axis

9. Discussion
Autor abstract words agrees with the
author's opinion
[29] S.B. Ong, K. Katwadi, X.Y. Kwek, N.I.
Ismail, K. Chinda, S.G. Ong, et al
We then turned to explore the underlying mechanism of DEX in
MI/R injury. It is well established that miRs show close participation
in the setting of MI/R injury via exerting effects on multiple key
elements
[15] X. Lv, P. Lu, Y. Hu, T. Xu. We found that DEX notably promoted miR-346-3p
expression, and miR-346-3p knockdown remarkably reversed the
protective effects of DEX on MI/R rat myocardium. In support of
these, previous work has verified the abnormal downregulation of
miR-346 in the MI/R injury rat model, and miR-346 overexpression
attenuates myocardial injury, as manifested by the reduced infarct
area and myocardial cell apoptosis
[39] Y. He, Z. Yang, J. Li, E. Li. DEX shows inhibitory effects on
expressions of NLRP3 and ASC in cardiomyocytes in heart-
related diseases

10. Conclusions
• Molecular biology is a great support for medicine in the
diagnosis of infectious diseases, autoimmune, orphan
and more, it allows to easily recognize the abnormal
expression of a gene or the production of a protein
that under normal conditions should not be, and based
on this analyze the impact it generates on the body
• Molecular biology is a great therapeutic target for the
prevention of diseases, allows the development of
proteins, hormones and other compounds that humans
cannot generate on their own because of their
condition and that we can thus administer and improve
their quality of life

12. Webgrafía
1. HTTPS://WWW.REVESPCARDIOL.ORG/ES-FISIOPATOLOGIA-DEL-DANO-
MIOCARDICO-POR-ARTICULO-13132052
2. HTTPS://WWW.REVESPCARDIOL.ORG/ES-DANO-MIOCARDICO-POR-
REPERFUSION-ARTICULO-
13067415#:~:TEXT=EL%20DA%C3%B1O%20MIOC%C3%A1RDICO%20POR%20R
EPERFUSI%C3%B3N,A%20LAS%20C%C3%A9LULAS%20PREVIAMENTE%20ISQU
%C3%A9MICAS.
3. HTTPS://WWW.ESCARDIO.ORG/STATIC-
FILE/ESCARDIO/EDUCATION/COURSES/BASIC%20SCIENCE%20SUMMER%20SCH
OOL/REVISED_PAGLIARO.PDF
4. HTTPS://WWW.NCBI.NLM.NIH.GOV/PMC/ARTICLES/PMC5412330/#:~:TEXT=MI
CRORNAS%20(MIRNAS)%20ARE%20A%20CLASS,CARDIOMYOCYTE%20HYPERTR
OPHY%2C%20AND%20HEART%20FAILURE.
5. HTTPS://WWW.NCBI.NLM.NIH.GOV/PMC/ARTICLES/PMC4503896/