Seminario biología molecular - Manuela Cano y Juliana Carvajal

1. Presented by: Manuela Cano Agudelo and Juliana Maria Carvajal Henao
3rd semester. Medicine. Universidad Pontificia Bolivariana. 2021

2. RENAL CANCER CELLS
It’s a disease were the malignant cells are located in the lining of the proximal convoluted tubules,
affecting the blood filtration.
Symptoms:
- Blood in the urine.
- Flan pain.
- Weight loss.
- Fever.
- High blood pressure.
- Anemia.

3. ANTI-PROLIFERATIVE AND ANTI-MIGRATORY PROPERTIES OF
COFFEE
KAHWEOL ACETATE and CAFESTOL
Two diterpenes or “essential oils” extracted from
coffee beans.
Implicated in a lot of diseases, mainly in dyslipidemias,
colorectal and prostate cancer, causing in most of them
positive effects.
Rats assays

4. “This study therefore,
investigated the effects of
kahweol acetate and cafestol on
human renal cancer ACHN and
Caki - 1 cells.”
OBJECTIVE

5. MATERIALES Y MÉTODOS
1 Ensayo de proliferación celular
Síntesis de DNA, determinando el aumento
exponencial y crecimiento celular.
Células renales cancerígenas + kahweol de
acetato y cafestol.
¿Estas sustancias podrían aumentar o
disminuir el recuento celular?

6. MATERIALES Y MÉTODOS
2 Ensayo de migración celular
Movimientos celulares sincronizados,
polarizados y en una sola dirección.
Células ACHN y Caki-1 + kahweol y cafestol.
¿Cuáles y cuántas células migraron?

7. MATERIALES Y MÉTODOS
3 Análisis de Western Blot
Detecta proteínas específicas con ayuda de
electroforesis y reconocimiento de anticuerpos.
¿Efecto de las estructuras proteicas del café
sobre la inhibición de la migración?

8. MATERIALES Y MÉTODOS
4 Ensayos de apoptosis
Muerte celular programada y dependiente
de energía; requiere de la acción de
caspasas.
Células anticancerígenas + kahweol y
cafestol.
¿Se favorece o inhibe la apoptosis?
Prueba de TUNEL

9. RESULTADOS

10. RESULTADOS

11. RESULTADOS

12. RESULTADOS

13. DISCUSSION
AUTHOR STATEMENT DOES IT
MATCH?
Motzer, R. J.
2018
“Recently, immune checkpoint inhibitors have become the
prominent treatment method for renal cancer, with immune
tolerance again becoming the main concern
in the treatment of renal cancer”
Iwamoto, H. 2019
“The present study examined the anti-cancer properties of
coffee kahweol acetate and cafestol and their synergistic
effects on two renal cancer cells, following our previous study
focusing on prostate cancer cells”
Woo, S. M. 2014
“Recently, several studies have shown that kahweol or
cafestol can regulate tumor cell activity and apoptosis-related
proteins through multiple targets, individually contributing to
the inhibition of renal cancer cell proliferation”

14. CONCLUSIONS
➔ Molecular biology allows the identification, on a structural and microscopical level, of
the organism physiological alterations, leading this towards a macroscopic
comprehension of the issue and helping the medical staff to define an accurate plan for
an effective treatment of pathologies.
➔ Molecular Biology has helped the study and recognition of the pathogenic agents
impact, such as microorganisms, on patients health, also how these agents interact
with the human organism at a molecular and structural level.
➔ Molecular biologists have performed studies based on genetical models of animals
which are genotypically and physiologically like human beings, this have leaded
towards a quick and excellent comprehension of causes, effects and treatments of
pathologies.

15. MAPA CONCEPTUAL: Manuela Cano Agudelo

16. MAPA CONCEPTUAL: Juliana Maria Carvajal Henao