High Performance Liquid Chromatography Systems and Detectors

WWW.SELON-GROUP.COM
High Performance
Liquid Chromatography

Shanghai Selon
Scientific Instrument Co.,Ltd
上海思科学器有限公司龙仪
LC-100 EX1600 HPLC EX1700
Ultra-speedy HPLC

Shanghai Selon
Domestic leading processing techniques make
every detail crafted, manifesting high quality from
inside out
Wider varieties of liquid phase configurations
provide multiple choices
Real-time display of unit operation &
comprehensively humanized consumable
replacement
LC-100 Liquid
Chromatography System

Shanghai Selon
Software system of the chromatography workstation
Two versions of software are available for selection to meet the needs of different users.
Workstation software–the Professional Version
The workstation is in accordance with the data GMP certification and FDA certification.It also meets the
requirements of data validity and safety, the system certification tool (IQ/OQ) and system suitability test (SST). The
highly efficient batch processing function enables the ongoing processing of instrument control, sequence collection
by the automatic sample injector, automatic integral calibration and output report.
Workstation software–the Simplified Version
The WS100 chromatography workstation adopts the RS232 serial port or USB connection. It uses all-digital signal
output to reversely control each part of the instrument comprehensively realizing automation and integration of the
system. Functions on the interface are simplified. Besides those serving routine analysis, there are also common
advanced functions including spectrum scanning and variable wavelength scanning. Professional integral processing
can still be done to the chromatograph during post-processing. The simplified interface design is more accessible to
users while satisfying the needs of the majority of them.

Shanghai Selon
Workstation software–the Simplified Version
•Gradient elution
Users can perform gradient elution simply and accurately by entering mobile phase ratios,
flow and transformation time into related lists. The software also provides corresponding
pump flow curves to help users set the gradients and acquire more visualized gradient
running progress.
•Spectrum scanning
The software provides the function of spectrum scanning in order to test the deuterium
lamp. Users can intuitively detect light intensities of the deuterium lamp under different
wavelengths and determine whether replacement of the lamp is necessary. They can also
judge the indication error of wavelength through positions of the 486nm and 656nm
points.
•Event relay
The event relay which adopts programmed control in time enables various functions users
need, including shifting the conversion valve to isolate and recycle the mobile phase.
These functions are not realized by any popular workstation at present.

Shanghai Selon
The P100 high pressure constant flow pump is a product widely praised
by its users.
This upgrade reserves the classic design of reciprocating parallel
pumps. It has the features of
high flow precision and small pressure pulsation.
Our original design of a one-way valve is an integral structure
comprising a valve core, a gem ball seat and a gem ball. It is
advantageous in its simple structure and good air-tightness.
The upgraded plunger rod is self-retracted. The seal ring can now be
changed easily without opening the instrument case.
Pumps with the function of post-column cleaning are specially
introduced to users using buffer salt systems. The design adopts two
seal rings
The seal rings of the plunger rod and the core of the one-way valve
can both be examined and
repaired conveniently, which facilitates maintenance of the product.
We provide our customers with more convenient service, smaller noise
and higher reliability.
Extended warranty periods and more comprehensive after-sales
service facilitate your tests.
Brand New P100
High Pressure Constant Flow Pump

Shanghai Selon
Model: P100 liquid delivery unit
Liquid delivery
system:
Double-plunger parallel
reciprocating transfusion,
autopulse suppression system
Flow rate: 0.001~9.999ml/min
Maximum
working
pressure:
42MPa
Flow rate
accuracy:
<±0.3%
(water,20C° ， 1ml/min,10MPa)
Qualitative
reproducibility:
<0.1 %( 1ml/min, 10MPa)
Quantitative
reproducibility:
<0.3 %( 1ml/min, 10MPa)

Shanghai Selon
Semi-prepared pumps,
high-pressure semi-prepared and prepared pumps
The semi-prepared LC systems are compatible with the requirements of both analysis and preparation, while high-pressure prepared LC
systems are mainly used for preparation purification and refinement of relatively small flow in laboratories.
Model: LC-100HP(40mL)semi-prepared
Flow rate: 0.01~40mL/min
Maximum working pressure: 0-25MPa
Flow accuracy: ±2%
Flow rate accuracy: RSD<0.1%
Model: high-pressure semi-prepared system
high-pressure prepared system
Flow rate: 0.01~120mL/min
0.01~50mL/min
Maximum working pressure: 0-40MPa
Flow accuracy: ±2%
Flow rate accuracy: RSD<0.2%
Parameter of semi-prepared LC systems
Parameter of high-pressure semi-prepared and prepared LC systems

Shanghai Selon
Binary high-pressure system
& quaternary low-pressure system
Applicable for gradient mixture of different
types
The binary high-pressure system equipped
with parallel pumps of high precision fully
ensures infusion steadiness and meets the
precision requirements of high-pressure
gradient analysis.
The quaternary low-pressure system is
equipped with all-imported four-channel
online degasification and a proportional
electromagnetic valve. It enables the
replacement, mixture and washing of various
mobile phases in a simple manner and
makes the operation even more convenient.

Shanghai Selon
Model LC100HP Binary
Mixed number 2 solvent
Flow rate accuracy
<±1% (water,
20℃1ml/min,10MPa)
Flow rate
reproducibility
<0.2 % ( 1ml/min, 10MPa)
Qualitative
reproducibility
<0.1 % ( 1ml/min, 10MPa)
Quantitative
reproducibility
<0.3 % ( 1ml/min, 10MPa)
Model LC100HP Quaternary
Mixed number 2~4 solvent
Flow rate accuracy
<±2% (water, 20℃,1ml/min,
10MPa)
Flow rate
reproducibility
<0.3 %( 1ml/min, 10MPa)
Qualitative
reproducibility
<0.2 %( 1ml/min, 10MPa)
Quantitative
reproducibility
<0.4 %( 1ml/min, 10MPa)
Binary high-pressure system Quaternary low pressure system Parameter

Shanghai Selon
UV-100 UV detector / UV-VIS100 UV-visible detector
This upgrade to the UV100 UV detector incorporates the design concept of ultrahigh-pressure
ultrafast LC instruments, avoiding distortion caused by system errors to the greatest extent.
The all-digital exchange system avoids the signal distortion and interference associated with
general UV detector signals caused by multiple analog-to-digital conversions.
The flow cell adopts the patented design of parallel bipyramid holes.The signal-to-noise ratio is
significantly improved compared to conventional flow cells. The test result turns out even better.
The deuterium lamps are fully upgraded to 2000h original imported deuterium lamps with
longer service life and better detection sensitivity. The wavelength ranges from 190 to 680nm.
Tungsten lamps are newly introduced as optional light sources. The wavelength ranges from
190 to 900nm, covering the entire range of visible light.
The minimum detection concentration is as low as 3×10- g/ml. Superior detection sensitivity
has been demonstrated.

Shanghai Selon
Model UV-100 UV detector UV-VIS100 UV detector UV-100S UV detector
Flow cell volume 8μL 8μL 6μL
Light source D2 lamp D2 lamp+ W lamp D2 lamp
Wavelength Range 190nm~680nm 190nm~900nm 190nm~680nm
Spectral Bandwidth 8nm
Noise 0.25×10- 5 AU *
Drift 0.4×10- 4 AU/hr. *
Wavelength Accuracy ±1nm
Wavelength precision <0.1nm

Shanghai Selon
Column oven
The temperature control parts and the solvent tray
are designed as integrated. This creates more
practical functions, nicer appearance, and more
stable temperature control, the range of which is 5
to 80 at room temperature. Good retention time
reproducibility is also demonstrated. Two columns
can be placed simultaneously to perform series
analysis. The whole system is under digital reverse
control and all operations can be done on
computer.
Model CO100
Temperature control principle Air circulation heating
Temperature setting range 5℃~80℃(At room temperature)
Temperature stability ±0.1℃
Temperature accuracy ±2℃(At 35℃)
Column number 2
Voltage 115V~230V, 50Hz~60Hz

Shanghai Selon
RI100 differential detector
The RI100 differential refractive index detector is a kind of general
detector widely applied in LC analysis. It responds to all solutes and
can therefore be used to detect ingredients such as macromolecular
compounds, carbohydrates and fat saturated hydrocarbons, which
cannot be detected with selective detectors. It can be widely used in
combination with Wufeng LC system in fields such as chemical
engineering, petroleum, medicine, and food industry to contribute to
research and production. The RI100 differential refractive index
detector is reliable in performance and easy to operate.
Detection range 0.25~512μRIU
Maximum flow rate 10ml/min
Temperature control 30℃~50℃
Drift <200nRIU/hr. *
Noise <2.5nRIU *
Flow cell volume 8μL
Linear range >600μRIU
Response time 0.1 ， 0.25 ， 0.5 ， 1.0 ， 1.5 ， 2 ， 3 ， 6
sec
Sensitivity DC 0~1V (2mV/μRIU, 8mV/μRIU)

Shanghai Selon
ELSD100 evaporative light-scattering detector
The evaporative light-scattering detector is a type of general
detector. Its universal detection method diminishes the
common hazards of conventional HPLC detection
approaches. Unlike UV and fluorescent detectors, ELSD
responds independently of the optical properties of
samples. Any sample can be detected free from the
influence of its functional groups as long as its volatility is
lower than the mobile phase. Its application covers all
compounds apart from those with low boiling points, and is
currently commonly used to detect materials without UV
absorption.
Sensing element Silicon photodiode
Temperature Range Room temperature~110℃
Atomizing gas Nitrogen is better, maximum 3.0L / min
Pressure operating range 15—90psi
Mobile phase flow rate 0—5mL/min
Analog Output 0-1 or 0-10mV full scale selectio
Signal Communications TTL / contact closure - error for LC pump shutdown
Select the operating parameters Keyboard input operation parameters
Display LCD

Shanghai Selon
 The EX1600 HPLC is a networked, intelligent,
automated, high precision HPLC developed by
Wufeng Instruments
EX1600 HPLC

Shanghai Selon
EX1600 networked control
• The EX1600 is equipped with
modern network technology to make
remote network control easy and
enable information sharing via local
area network. It allows you to handle
lab operations and data processing
via the network in real-time from
your lab or office.
• Combines with the auto-sampler to
form a safe highly efficient
unattended lab.

Shanghai Selon
•• All-newAll-new EX1600QP quaternary gradient unit combines with the
Arcus5
auto-sampler to automate your lab work. Four mobile phases can be
mixed at any desired ratio to make your experiments easier.

Shanghai Selon
All-new Digital Motor-driven
High Pressure Constant-flow Pump
• Innovative digital linear motor technology subverts traditional cam driven quaternary gradient
technology. This all-new technology allows the infusion unit’s piston rod displacement to be
controlled digitally. It is mated with a millisecond new-type motor for positive and negative
switching, which makes it possible to open and close solenoid valves in up to four channels.
Analysts will enjoy optimal application experience with any mixing proportion.
Digital displacement
monitoring module

Shanghai Selon
All-new digital type VS. traditional cam type
• Traditional cam-based quaternary gradient products have some inherent defects. How does the piston rod move?
A flexible conveyor belt is driven by the movement of a step motor to actuate the cam and turn the piston rod in
reciprocating motion.
• The working process is complicated, since the cam profile is not a standard Archimedes line. This makes it difficult
to mass produce and process. Also, the step motor’s positive and negative switching is based in seconds, so
infusion must be compensated with a jack-head pump. This leads to an inaccurate proportion of diverse infusion
channels and marked deviation.
• The linear motor’s travel control is determined by a driving pulse number, while the positive and negative phase
are controlled by high and low electrical levels. It is possible to control deviation of displacement per turn of the
pushing shaft within 0.01 mm, and the turning angle of the step motor connects precisely with displacement of the
related piston rod. The turn angle is controlled by a digital pulse circuit, and the piston rod displacement is directly
related to the linear digital value. This allows you to control the switch on/off times for the four solenoid valves A,
B, C and D, to make zero positioning more accurate. This ensures precise control of the quaternary mobile phase
proportion.
China’s First All-new Digital Quarternary Gradient Unit - Redefining Quarternary Gradient Precision

Shanghai Selon
All-new Digital Type VS. Traditional Cam Type
Traditional quaternary gradient technology
has fatal defects
Advantages of the new digital
quaternary gradient pump
Large deviation in mobile phase output
proportion
Cannot handle micro flow applications
Unstable instrument quality due to poor
manufacturing precision of internal components
Commonly used static mixers are ineffective
Requires manual mixing since it is difficult to handle
small proportional mixing of mobile phases
Accurate control of the mobile phase ratio
Dynamic mix, outstanding mixing effect
No manual mixing for small mixing proportions
Built-in degassers are effective and simple to
operate with low failure rates
Digital motor provides stable quality

Shanghai Selon
Solvent Tray
最简化溶剂托盘
双切换阀溶剂托盘
单切换阀溶剂托盘
Infusion Unit
EX1600HP High Pressure Infusion Unit
EX1600LP Quaternary Gradient Infusion Unit
EX1600PP Semi-preparative Infusion Unit
Detector Unit
EX1600UV I UV Detector
EX1600UV II UV-visible D2 Lamp with W Lamp Detector
EX1600RI Differential Refractive Index Detector （ * ）
Column Oven
EX1600CO I 普通型
EX1600CO II 加制冷用型热两
Sampler
Arcus5 Auto-sampler （ * ）
 Chromatographic Column (Exformma)
EX-CLARITY Professional
Chromatographic Management Workstation
EX1600 Liquid Chromatography Composition Unit

Shanghai Selon
Automatic post-
column rinse
function
Automatic on-off
function
Status monitoring and
failure warning
Leakage alarm
function
Man-machine
interaction on
time program
setting
Advantages of EX1600 infusion unit
EX1600 Infusion Unit

Shanghai Selon
（ 1 ） Flow rate range ： 0.001 ～ 9.999 ml/min 以 0.001 ml/min 步
流量。长调节
（ 2 ）流量定性差稳误 SR: 小于 0.3%
（ 3 ）流量定差设值误 Ss: 小于 ±2%
（ 4 ） Maximum operating pressure ： 42MPa (0.001~9.999ml/min)
（ 5 ）力性和准度：示力差小于压线确显压误 ±0.5 MPa (0~42MPa)
（ 6 ） Pressure fluctuation ： Below 0.1 MPa (flow rate1ml/min,
Pressure 5~10 MPa)
（ 7 ）的密封性：力泵压为 42 MPa ，时 10min 内力降小于压 0.5 MPa.
Specification of EX1600 Infusion Unit

Shanghai Selon
Used for high capacity tests and
analysis or to prepare and extract
microscale products.
Prolong related component lifespan of
the system.
EX1600 Liquid Chromatography Composition Unit-Infusion Unit
EX1600PP Semi-preparative Infusion Unit

Shanghai Selon
Significantly increases the signal-to-noise
ratio compared to traditional flow cells, and
improves detection performance.
Automatically determines light intensity
and energy,
Equipped with leakage auto alarm
Intelligent function
Linked with modern network technology,
Connected with Intranet of users via LAN
interface on rear panel of UV detector
Remote control
The deuterium lamp lasts
2,000 hours.
Long-life deuterium lamp
Built-in online components monitor
liquid leakage in the detector, and
warn you immediately if leaks are
present in any key components such
as the flow cell.
Process monitoring function
Digital switching system
Avoid signal interference and distortion.
Common UV detectors require multiple
digital conversions
EX1600 detector unit
Advantages of the EX1600 UV detector

Shanghai Selon
（ 1 ） Wavelength range ： 180 － 700nm
（ 2 ） Wavelength accurancy ： 8nm
（ 3 ） Baseline noise ：≤ 2× 10-5
AU （ methanol 、 1ml/min 、 254nm 、 20℃ ）
（ 4 ） Baseline drift ：≤ 4 × 10-4
AU/hr （ methanol 、 1ml/min 、 254nm 、 20℃ ）
（ 5 ） Minimum detectable concentration ：≤ 3 × 10 － 9
g/ml
(naphthalene/methanol standards )
（ 6 ） Wavelength accuracy ：≤ ±1nm
（ 7 ）波重性差：于长复误优 0.2nm
（ 8 ）性范：于线围优 104
EX1600 Detector Unit
Specification Of EX1600 UV Detector

Shanghai Selon
Optimize temperature control,
shortening the stabilization time after
power-on, resulting in a more stable
baseline
The auto-start function completes the
complex operation of replacing the
reference cell solution to the baseline
stability check.
The EX1600WS Intelligent Networked
Chromatography Workstation is highly
automated.
EX1600RI Differential Refractive Index Detector

Shanghai Selon
EX1600WS Intelligent Networked Chromatographic Management Workstation
EX1600 Workstation

Shanghai Selon
Features No.1 strong control ： The workstation can automatically
identify each instrument unit and set parameters for control. This makes it
possible to perform multi-channel gradient elution, wavelength scanning, and
switch-on-event relay. The workstation can display sample chromatogram,
pressure-time curves for infusion units, flow-time curves for multiple pumps, and
wavelength scanning curves, etc. in real-time.
EX1600 Workstation
Characteristics of EX1600WS Intelligent Networked Chromatographic Management Workstation

Shanghai Selon
Features NO.2 establish a remote control platform
LAN global monitoring, through the web server, you can view the current
status of each chromatographic system in the entire LAN, access to first-hand
information for each instrument. Real-time view of the test analysis of the
current data, the first time to obtain sample analysis results.Data Management
Workstation
The EX1600WS chromatographic management workstation is a remote
data management platform. It uses digital communications to save raw data, and
then its powerful post-processing function provides real results.
Characteristics of EX1600WS Intelligent Networked
Chromatographic Management Workstation

Shanghai Selon
Features No.2 to establish wavelength scanning and variable wavelength Establish wavelength scanning
function
Establish wavelength scanning function
Wavelength scanning is: After the sample is injected into the instrument, the computer issues a command
to stop the pump, leaving the sample in the flow cell and the detector for full wavelength scanning. This gives you
an idea of which wavelength the sample has the highest absorbance, and you can use that wavelength in your
next analysis to achieve the best possible results.
Establish variable wavelength scanning function
The EX1600WS Chromatographic Workstation allows you to change the wavelength of the current test
while analyzing the sample by programming the wavelength-time program. Better results can be obtained by
changing the wavelength of the detected wavelength, particularly with respect to the retention time In the case of
complex components, a single wavelength can not meet the needs of detection. By changing the detection
wavelength, a better detection result can be obtained.
Characteristics of EX1600WS
Intelligent Networked Chromatographic Management Workstation

Shanghai Selon
EX1600 VS LC-100
LC-100PLUS EX1600
Intelligent The first digital computer intelligent
full-controlled liquid
chromatography system
A higher degree of intelligence, a higher
degree of humanity
Stability LC-100PLUS already has nearly a
thousand users
EX1600 is the introduction of foreign
technology, all aspects of performance in
the world advanced level simultaneously
Automation High degree of automation, easy to
operate
A higher degree of automation, a high
degree of human nature, easy to operate
Configuration Configuration Fully meet the
complex sample system of liquid
chromatography
Configuration flexible, diverse, for more
demanding chromatographic analysis
EX1600 Product Comparison

Shanghai Selon
 The EX1700 infusion unit is equipped with an auto-compensation
function for fluid compression. This ensures constant flow even
when the infusion pump pressure changes. Deviations in the
infusion pump settings can be controlled to within 2%, and
deviations in flow stability can be controlled to within 0.1% while
operating below 10 MPa or above 40 MPa.
EX1700 Ultra-speedy HPLC

Shanghai Selon
 The all-new high pressure binary mixer mixes fluids based on forward-
backward helical cross-mixing principles to deliver superb mixing results,
lower working pressure, and fewer blockages.
 Multiple series systems available to meet diverse mixing requirements,
encompassing a variety of different mixtures and mixing volumes.

Shanghai Selon
 Gradient working graph of the EX1700 infusion unit
Mobile phase:
A phase: water + 0.3%
acetone
B phase: Water
Flow rate: 1 mL/min
Pressure: 10.5 MPa (damper
tube)
Mixed volume:
240 μL (MX3-80)
Wavelength: 254 nm
EX1700 infusion unit

Shanghai Selon
 The EX1700UV UV detector micro flow cell (Volume 1.0 μL) and high speed
A/D data acquisition card (100 samplings per second) enhance fast-peak
detecting ability and waveform accuracy. It can measure of a minimum full
width at half maxima within 0.5 seconds.

Shanghai Selon
Impact of Flow Cell Volume
 The EX1700 S-HPLC®
uses microflow cells (1.0 μL) and high-speed
A/D data acquisition card.
Offers higher resolution than traditional 8 μL flow cells and 5 μL semi-
micro flow cells.
EX1700 micro flow cell
(good resolution)
Standard flow cell (can’t resolve) 5μL semi-micro flow cell (poor
resolution)
EX1700 detector unit

Shanghai Selon
 EX1700 oven unit can hold 2-3 columns for ultra-efficient use or long
columns for conventional chromatography 1 root.
EX1700 Column Oven

Shanghai Selon
• Analytical chromatogram of common HPLC
• Analytical chromatogram of EX1700 S-HPLC
The EX1700 cuts analysis time by more than one hour and uses much less solvent with no significant loss in column
efficiency or resolution.
Analysis of ginsenoside——isocratic conditions
EX1700 Applications

Shanghai Selon
• Analytical chromatogram from common HPLC
Rg1=52.695min
N ＝ 359797
Re=53.272min
N ＝ 395698
Resolution=1.67
Rg1=9.932min
N ＝ 108251
Re=10.138min
N ＝ 139067
Resolution=1.80
Chinese pharmacopeia (2010)
P8, “ginseng” item specifies the
gradient condition: A － water; B
－ acetonitrile;
Time (min) A% B%
0 81 19
35 81 19
55 71 29
70 71 29
100 60 40
HPLC column C18,5um,4.6 × 250mm
HPLC column C18,1.8um,4.6×50mm
Rb1 ＝ 87.853min
N ＝ 951348
Rb1 ＝ 17.963min
N ＝ 605020
The EX1700 can complete this analysis in just 20 minutes compared to 100 minutes for most common
HPLCs. This greatly simplifies gradient conversion.
Analysis of Ginsenoside——Gradient Conditions
EX1700 Application

Shanghai Selon
• Analytical chromatogram from common HPLC
3.605min
21.463min
HPLC column: C18,5um,4.6×250mm
HPLC column: C18,1.8um,4.6×50mm
Chinese pharmacopeia (2010) P105
water:acetonitrile ＝ 30:70
The EX1700 can complete this analysis in just 4 minutes compared to 20 minutes for most common HPLCs.
Analysis of Levonorgestrel Tablets
EX1700 Application

Shanghai Selon
 The Arcus 5 auto-sampler targets common HPLC, while the Arcus 7
auto-sampler is ideal for UHPLC ultra-high pressure liquid
chromatographs. It can be used in conjunction with any brand of
HPLC. It is a top-end high performance auto-sampler for HPLC.
Arcus Series auto-samplers

Shanghai Selon
User-friendly design, easy
handling and complete functions
Simple handling, easy control and
complete records
Reduce residues to less than 0.005% with
diverse optimization measures. Ensure high
efficiency with repetitive sample injections.
Shortened sample injection cycle and
reduced cross contamination
Features multiple protective
measures to reduce damage to
instruments caused by improper
operation.
Super effective self-protective measures
Compatible with over 400 types of
HPLCs on the market
Fully controlled by computer,
and compatible with any HPLC
Uses a metering pump design
with a gem-based rod as its
piston rod, the high pressure
piston seal has a long service life
of over 1 million cycles
Super long service for
quick-wear parts
Ensures high precision for sample
injections
Design of high-precision
metering pump
Arcus Series Auto-samplers

Shanghai Selon
• Reproducibility with 6 injection iterations (injection volume = 2 µL)
part2µLL1C
2012-11-09 11-53-09_1
　
A: 312.824
H: 40.10
C: 100.0000
Rt:7.198
part2µLL1C
2012-11-09 12-02-06_2
A: 314.345
H: 40.22
C: 100.0000
Rt:7.191
part2µLL1C
2012-11-09 12-11-05_3
A: 313.096
H: 40.23
C: 100.0000
Rt:7.197
part2µLL1C
2012-11-09 12-20-02_4
A: 312.535
H: 40.10
C: 100.0000
part2µLL1C
2012-11-09 12-29-00_5
A: 312.365
H: 40.13
C: 100.0000
Rt:7.197
part2µLL1C
2012-11-09 12-37-58_6
A: 312.565
H: 40.07
C: 100.0000
Rt:7.198
AVERAGE
A: 312.955
H: 40.14
C: 100
Rt:7.20
RSD 　
A: 0.23%
H: 0.17%
C: 0.00%
Rt:0.04%
Table 1 (Continued)Table 1

Shanghai Selon
• Basic Features and Functions
Features a high-precision power-driven
injection valve, shorter delay time,
reduced dead volume, and produces
virtually no residue.
Patented design, valve core made of
super wear-proof materials, longer
service life, specially designed waste
tubes reduce impact on sampling tubes.

Shanghai Selon
Stable operation and accurate
positioning, trays can be freely replaced
and identified automatically.
Freely combine trays containing 2mL, 4mL
or 10mL vials, 108 vial maximum capacity
to meet your daily work requirements.
 Basic Features and Functions

Shanghai Selon
Better positioning precision and
quantitative reproducibility, sets
sampling speed based on the
variety of samples, linear
sampling ensures validity and
reliability of data analysis.

Shanghai Selon
Super Effective Self-protection
The Arcus 5 auto-sampler employs multiple patented technologies
in design and R&D, Multiple protection measures reduce damage
to sample injectors due to misuse.
 Injection needle protection unit—sampler will automatically stop
to protect injection needle from damage when it fails to inject vial
as normal.
 If the vial detector does not detect a vial, the system will
automatically stop or skip to the next position based on user defined
settings.
 Continuously monitors tube status—if the injection needle tube is
blocked, it stops running immediately and sounds an alarm. This
prevents unexpected failures due to blockage.
 To prevent inaccurate sample injections and other problems caused
by fluid leakage, the system is equipped with a leakage detection
unit that warns the user immediately in the event of leakage.

Shanghai Selon
Superb injection reproducibility prevents
uncertainty due to user error
Excellent injection linearity to meet your
injection volume requirements
Linearity is greater than 0.9999

Shanghai Selon
Ultra-low sample residue, so there is no
effect on subsequent injections.
File name 1
full residual 25µL(blank)
2011-12-01 10-11-18
　
　
　
A: 0.106
H: 0.03
C: 100.0000
Rt:4.938
full residual 25µL(sample)
2011-12-01 10-02-26
　
　
　
A: 5258.022
H: 886.12
C: 100.0000
Rt:4.966
Residual 2.01597E-05
 Basic features and functions

Shanghai Selon
• Basic flow path of Arcus Arcus Series auto-samplers
•
Sampler Flow Path
High Pressure
Flow Path

Shanghai Selon
 Arcus auto-samplers come in three sample injection ways,
rendering flexibility mated with different rinsing methods.
■ Full loop injection: Best accuracy and reproducibility.
■ Partial loop injection: Favorable sample injection reproducibility
and best flexibility.
■ No waste injection: Zero sample loss and flexibility.
All three sample injection methods mentioned above can utilize
the bubble reduction function to reduce rinsing volume, and
prevent cross-contamination when changing samples.

Shanghai Selon
• Full loop sampling mode (full loop)
■ 置换

Shanghai Selon
• Full loop sampling mode (full loop)
■ Sampler

Shanghai Selon
• Partial loop sampling mode (part loop)
■ Sampler

Shanghai Selon
•
No waste sampling mode (ul pickup), default position
■ 置换

Shanghai Selon
• No waste sampling mode (ul pickup), random position
■ Sampler

Shanghai Selon
•
No waste sampling mode (ul pickup), random position
■ 填充

Shanghai Selon
•
No waste sampling mode (ul pickup), random position
■ Sampler

Shanghai Selon
Convenient operation,
Easy to visualize work flow,
All operations can be
conducted via computer
 Arcus Workstation

Shanghai Selon
Diverse injection and rinsing
methods to suit your
application.

Shanghai Selon
Frees you to set multiple
sequences and combine all kinds
of injection methods.
Real-time feedback about system
status and performance.

Shanghai Selon
Complete self-diagnosis and recording
Fast power-on, self-checking, quick positioning, and coordinate
axis calibration, detects working status of metering pump
Records high pressure injection valve usage time
Records piston seal usage time
Records metering pump valve core usage times
Injection event record

Shanghai Selon
 Working temperature: 10 ~ 40 ℃
 Storage temperature: -25 ~ +60 (no frosting on the surface)℃
 Sample Loading: Basic Tray
 Vial height: H ≤ 48mm (including septa and caps);
 Quantitative ring volume: 1 ~ 120μL (expandable, factory standard configuration: 50μL);
 Metering pump volume: 200μL;
 Injection mode: full quantitative loop injection, some quantitative ring injection, sample
lossless injection
2ml × 54 bits (factory standard configuration);
10ml × 15 digits
4ml × 35 bits
1 ml × 98 bits (Arucs 7)
Each side can choose a type of tray, can be placed on both sides of any type of
tray, the system automatically determine the tray size; Arcus 5 up to 108, Arucs 7
up to 196
Arcus Series Autosampler Unit

Shanghai Selon
 Quantitative repeatability:
 Residual: ≤0.05% (execute a cleaning program)
 Volume: 450mm × 300mm × 330mm
 Weight: 23kg (without package)
Full quantitative ring injection: RSD6 ≤ 0.3%
Partial quantitative loop injection: RSD6 ≤ 0.5% (injection volume ≥ 10μL)
(Actual test, RSD6 <0.5% at 2 μL injection volume)
No loss of injection: RSD6 ≤ 1.0% (injection volume ≥ 10μL)
Arcus Series Autosampler Unit

Shanghai Selon
 The EX-Clarity chromatographic workstation was co-developed by Wufeng Instruments
and DataApex, the world’s leading software runner. The workstation was developed
according to all-new design concepts to meet a string of data validity and data safety
requirements stipulated by the Installation Qualification/Operational Qualification
(IQ/OQ) and System Suitability Test (SST), etc. Efficient batch processing streamlines
instrument control, auto-sampler sequence acquisition, automatic integration
calibration, and output reporting. It also features a number of useful functions, including
post-processing, chromatogram comparison, re-calibration, data input/output, and
three-dimensional chromatogram processing.
 Different methods for diverse vials
 Support for plug-ins that transform it from a common HPLC into a UHPLC
EX-Clarity Chromatographic Workstation

Shanghai Selon
• References 1: Application of M132 Clarity in regulatory environments
• References 2: D019 Clarity software meeting requirements for CFR 21 Part 11
• References 3: EU GMP/GDP Codification—Chemical Industry Press
Clarity Professional conforms to environmental regulations
- CFR 21 Part11 - GMP/GLP guidelines

Shanghai Selon
◆ Personnel: Only qualified personnel who have completed training and examination may participate in
GMP activities. These personnel are required to complete regular training sessions and examinations.
(Usually valid for one year, but after upgrading corresponding SOP, a new round of training and
examination is required)
◆ Instrument:
1. Safety (References 3—Appendix 11 Page 307)
1.1 Related personnel have exclusive authorized accounts that cannot be accessed without authorization.
1.2 Authorized accounts are reviewed regularly.
<Reference> Environmental regulations 1 & 2 apply to the full-function version of Clarity
2. Regular assessment (References 3—Appendix 11, Page 307 & Appendix 15, Page 357-360)
2.1 Regular instrument certification (Completed by factory, e.g.: 3Q certificate)
2.2 Conduct regular internal PM (preventive maintenance plan) and instrument verification (performance
tests on diverse instrument components)
<Note> PM operation: These tasks are easy, but related PM operations must be recorded in a timely
fashion. Included dusting equipment, rinsing tube and deposing of liquid waste, etc.
Verification: Relatively complicated, should be completed by professionals, for example: pump - flow rate
test, auto-sampler - injection volume accuracy test, column oven - temperature test, detector - DAD
wavelength scanning test, etc.
Requirements on personnel and analysis instrument to carry out GMP

Shanghai Selon
• Traceability of GMP related modifications and deletions
(References 3—Appendix 11 Page 307)
Audit trail: Emphasizes traceability of data manipulation (all changes can
be recorded, and require specified reasons)
Access control: Digital signature ensures signature validity
<Reference> Environmental regulations 5 & 6 apply to the full-function
version of Clarity
• Ensure GMP-related data integrity (Reference 3—Appendix 11, Page
306-307 ）
Data storage: Keep intact and perform regular back ups (automated)
Archiving: Effectively store lab data over the long term, must be restorable
(by IT professionals).
Requirements on Clarity software to implement GMP

Shanghai Selon
 GMP Related Changes, Deletions Traceable (Ref. 3 - Appendix 11 Page307)
•         Audit Trail: Focus on Data Traceability (Any action can be recorded, any changes need to explain the
reasons)
•         Permission Control: Digital Signature Ensures the validity of the signature
•        <Reference> Clarity full-featured version meets the environmental specifications 5 & 6
•
 GMP-related data ensures completeness (Ref. 3 - Appendix 11 Page306 & 307)
•         Data storage: complete, regular backup (auto-complete)
•         Filing: long-term effective preservation of experimental data, recoverability (by IT professionals)
Implement GMP requirements for Clarity software

Shanghai Selon
Clarity Professional Version Comply with environmental regulations
To validate Clarity's environmental compliance options, users must complete the
next three steps.
• Step 1) Set the Windows operating system user account permissions, please refer to
Microsoft's operation System manual to set.
• Step 2) Set Clarity software user account, the user can click on the "user account" Define
different levels of user operating authority. (See Figure 1.1, Figure 1.2)
• Step 3) Set the GLP option of Clarity software to validate the regulatory environment.(See
Figure 2.1, Figure 2.2)
图 1.1 图 2.1

Shanghai Selon
Clarity Professional Version
Comply with environmental regulations
图 1.2
用号定框户账设对话
图 2.2
GLP 定框选项设对话

Shanghai Selon
• Clarity software provides IQ reports for installation validation.

Shanghai Selon
• Clarity software provides OQ wizard for operational validity check.
If you want to check not only the validity of the software, but also the validity of your AD acquisition,You
must purchase other modules to complete the appropriate inspection.

Shanghai Selon
Audit Trail Functionality:Audit Trail Functionality: The trace log of daily operations and method changes, anyThe trace log of daily operations and method changes, any
changes must state the reason for the change.changes must state the reason for the change.

Shanghai Selon
• Clarity software supports digital signatures, users can use their own operating
system authorized digital signature certificate

Shanghai Selon
Thank YouShanghai Selon Scientific Instrument Co.,Ltd
Address: Room.407, NO.600, North Bao xing Road,
Jingan District, Shanghai, China.
Tel: 86-21-35100022/86-21-65416501
Email: sales@selon-group.com

High Performance Liquid Chromatography Systems and Detectors

Recommended

Recommended

More Related Content

Similar to High Performance Liquid Chromatography Systems and Detectors

Similar to High Performance Liquid Chromatography Systems and Detectors (20)

Recently uploaded

Recently uploaded (20)

High Performance Liquid Chromatography Systems and Detectors