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MAHESH VASANT SABLE
Near L.I.C. Office, Dhoki Road, Kallam, Ta – Kallam Dist – Osmanabad. Maharashtra – 413507.
Contact: +917720810111; Email: mahesh.v.sable@gmail.com; Date of Birth: 21st June, 1989
Resume Details
Experience:
Presently Working in:
EMBIO RESEARCH CENTRE,
Division of EMBIO Ltd,
Mahad Industrial Area,
Mahad. Dist-Raigad, Maharashtra. India-Pin: 402309.
Designation: Research Officer.
From: 11th Nov 2013 till date.
Also worked as
Production officer
At Microbax India Ltd, Hyderabad.
( 06 Aug 2012 – 31 Oct 2013 )
Job profile:
Fermentation – Scale up & Process Optimization
1. To carry out upstream and downstream processes of fermentation batches (bacterial,
yeast).
2. Experience in, scale up of yeast fermentation in shake flask – 3D shaker-
Fermenters (200ml to 150 L to 4 KL).
3. Having experience in inoculum development, media optimization & strain
improvement.
4. Scale up & process optimization of E.Coli AT 12
5. Process development for highest biomass production of E.Coli AT 12.
6. Supervision of Batch Charging, Media Sterilization, aseptic transfers.
7. Process Modeling, Monitoring & Statistical Data Analysis.
8. Operating equipment as per process need.
9. Expert hand at all utility works( Air pressure, Steam pressure, Vaccum, Chilling &
Cooling line), Probes , troubleshooting activities of experiments and investigation for
process Failure.
10.Follow up with service departments to get the job done in time
Biotransformation
1. Yeast fermentation experience includes, optimization of media and other parameters for
PAC production with the help of biotransformation of Benzaldehyde.
Probiotics work
1. Probiotics production on lab scale in shake flask studies and fermentor.
2. Media optimization for spore forming bacteria.
3. Seed preparation, Sterility testing, pH and OD Checking, Direct microscopic
Count, Staining etc.
4. Probiotics handled:
Bacillus clausii Bacillus coagulans
Lactobacillus plantarum Lactobacillus acidophilus
Lactobacillus fermentum Saccharomyces boulardii etc.
Other responsibilities.
1. Enzymes & Extracellular Metabolite study. Isolation of enzymes from Bacterial
& yeast cells & development of enzyme assays, e.g Pyruvate Decarboxylase,
Invertse (yeast).
2. Determinig the real and projected cost of the product.
3. To perform mixing experiments in fermentors and determine kla.
4. Literature Review (search research papers from Chemical Abstract.)
5. Daily calibration of pH Meter.
6. Extra job Responsibility: Microbial culture maintenance
Key techniques :
1. Have very good knowledge practical as well as theoretical of
microbiological skills & techniques.
2. Expert hand at some of microbial inoculation techniques &
analytical instruments.
3. Having good computer knowledge.
Project Undertaken
EDUCATIONAL CREDENTIALS
YEAR EDUCATIONAL
QUALIFICATION
BOARD/
UNIVERSITY
INSTITUTION PERCENTAGE OF
MARKS
2009-11 M.Sc. Biotechnology Bangalore
University
Capital college
Bangalore
69.10%
2006-09 B.Sc. Biotechnology Pune University R.J.S.P.M’s
college, Pune
53.00%
2005-06 12th
class Maharashtra
State Board
Dayanand college,
Latur
80.17%
2003-04 10th
class C.B.S.E. Board J.N.V. Tuljapur 70.80%
Project title Description Organization Year
Antibacterial activity of
dried plant parts on
pathogenic bacteria
Checked antibacterial activity of
Amla and Hibiscus against
Staphylococcus and
Pseudomonas.
R.J.S.P.M , College ,
Pune.
2008-09
Declaration:
I hereby declare that the information furnished above is true and correct to the best of
my knowledge and belief.
PLACE:
DATE: Mahesh Vasant Sable
Personal Details
Name Mahesh Vasant Sable
Date of Birth 21st June 1989
Gender Male
Blood Group A+ve
Marital Status Married
Languages known English, Hindi & Marathi
Hobbies Playing Cricket & listening Music

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mahesh cv

  • 1. MAHESH VASANT SABLE Near L.I.C. Office, Dhoki Road, Kallam, Ta – Kallam Dist – Osmanabad. Maharashtra – 413507. Contact: +917720810111; Email: mahesh.v.sable@gmail.com; Date of Birth: 21st June, 1989 Resume Details Experience: Presently Working in: EMBIO RESEARCH CENTRE, Division of EMBIO Ltd, Mahad Industrial Area, Mahad. Dist-Raigad, Maharashtra. India-Pin: 402309. Designation: Research Officer. From: 11th Nov 2013 till date. Also worked as Production officer At Microbax India Ltd, Hyderabad. ( 06 Aug 2012 – 31 Oct 2013 ) Job profile: Fermentation – Scale up & Process Optimization 1. To carry out upstream and downstream processes of fermentation batches (bacterial, yeast). 2. Experience in, scale up of yeast fermentation in shake flask – 3D shaker- Fermenters (200ml to 150 L to 4 KL). 3. Having experience in inoculum development, media optimization & strain improvement. 4. Scale up & process optimization of E.Coli AT 12 5. Process development for highest biomass production of E.Coli AT 12. 6. Supervision of Batch Charging, Media Sterilization, aseptic transfers. 7. Process Modeling, Monitoring & Statistical Data Analysis. 8. Operating equipment as per process need. 9. Expert hand at all utility works( Air pressure, Steam pressure, Vaccum, Chilling & Cooling line), Probes , troubleshooting activities of experiments and investigation for process Failure. 10.Follow up with service departments to get the job done in time
  • 2. Biotransformation 1. Yeast fermentation experience includes, optimization of media and other parameters for PAC production with the help of biotransformation of Benzaldehyde. Probiotics work 1. Probiotics production on lab scale in shake flask studies and fermentor. 2. Media optimization for spore forming bacteria. 3. Seed preparation, Sterility testing, pH and OD Checking, Direct microscopic Count, Staining etc. 4. Probiotics handled: Bacillus clausii Bacillus coagulans Lactobacillus plantarum Lactobacillus acidophilus Lactobacillus fermentum Saccharomyces boulardii etc. Other responsibilities. 1. Enzymes & Extracellular Metabolite study. Isolation of enzymes from Bacterial & yeast cells & development of enzyme assays, e.g Pyruvate Decarboxylase, Invertse (yeast). 2. Determinig the real and projected cost of the product. 3. To perform mixing experiments in fermentors and determine kla. 4. Literature Review (search research papers from Chemical Abstract.) 5. Daily calibration of pH Meter. 6. Extra job Responsibility: Microbial culture maintenance Key techniques : 1. Have very good knowledge practical as well as theoretical of microbiological skills & techniques. 2. Expert hand at some of microbial inoculation techniques & analytical instruments. 3. Having good computer knowledge.
  • 3. Project Undertaken EDUCATIONAL CREDENTIALS YEAR EDUCATIONAL QUALIFICATION BOARD/ UNIVERSITY INSTITUTION PERCENTAGE OF MARKS 2009-11 M.Sc. Biotechnology Bangalore University Capital college Bangalore 69.10% 2006-09 B.Sc. Biotechnology Pune University R.J.S.P.M’s college, Pune 53.00% 2005-06 12th class Maharashtra State Board Dayanand college, Latur 80.17% 2003-04 10th class C.B.S.E. Board J.N.V. Tuljapur 70.80% Project title Description Organization Year Antibacterial activity of dried plant parts on pathogenic bacteria Checked antibacterial activity of Amla and Hibiscus against Staphylococcus and Pseudomonas. R.J.S.P.M , College , Pune. 2008-09
  • 4. Declaration: I hereby declare that the information furnished above is true and correct to the best of my knowledge and belief. PLACE: DATE: Mahesh Vasant Sable Personal Details Name Mahesh Vasant Sable Date of Birth 21st June 1989 Gender Male Blood Group A+ve Marital Status Married Languages known English, Hindi & Marathi Hobbies Playing Cricket & listening Music