1. Kenneth M. Straub, Ph.D.
Work address: Roche Palo Alto, LLC Home address: 872 Brookline Drive
MS R6-007 Sunnyvale, CA 94087
3431 Hillview Avenue tel (408) 746-2950
Palo Alto, CA 94304 email: kennethstraub79@gmail.com
tel (650) 855-5067
email: kenneth.straub@roche.com
SUMMARY
Senior scientist specializing in applications of mass spectrometry to protein and peptide analytics.
Fifteen years experience in characterization of recombinant proteins and peptides by advanced MS
technologies; well-versed in bioinformatics and high throughput/ lab automation. Team player
providing analytical support to project teams in a complex multi-disciplinary Pharma R&D
environment. Experienced at operating under GMP/GXP regulations. Supervision of Ph.D. and
MS/BS level staff.
PROFESSIONAL EXPERIENCE
ROCHE PALO ALTO LLC, Palo Alto, California 1995 — present
Senior Scientist
Developed and managed core analytical group (Mass Spectrometry and Protein Analysis Lab)
providing support for Molecular and Protein Sciences Department and for Medicinal Chemistry,
Process Chemistry and Pharmaceutics. Managed and developed technologies for characterization of
recombinant proteins to support high throughput expression effort. The lab provided the following
analysis methods:
• Protein identification and peptide mapping via LC-tandem MS and MALDI-tandem MS
• Characterization of post-translational modifications (phosphorylation, acetylation,
gylcosylation, etc.) in isolated proteins
• High accuracy intact MW determination for characterization of covalent and non-covalent
ligand binding to isolated proteins
• Quantitative expression analysis (ICAT/ iTRAQ) for target proteins
• N-terminal (Edman) microsequencing
• Web-based data analysis and reporting of results
SYNTEX RESEARCH, Palo Alto, California 1987 — 1995
Principal Scientist
Staff scientist in the Analytical Research group, providing structure elucidation and quantitative assay
development for Medicinal, Process, and Pharmaceutical Chemistry groups.
• Structure characterization of small molecules via mass spectrometry (LSIMS, LCMS, GCMS)
• High resolution/ accurate mass analysis
• Development of self-service (walk-up) instrument analysis and web-based reporting of data
EDUCATION
• B.S., Chemistry, Amherst College, Amherst, MA
• Ph.D., Chemistry, University of California, Berkeley
• Post-doctoral studies, University of California, San Francisco

2. Résumé – Kenneth M. Straub
TECHNICAL PROFICIENCIES
Broad background in a variety of protein chemistry and proteomics technologies, including peptide
mapping by both MALDI-TOF/TOF-MS and LC-MS/MS, characterization of post-translational
modifications, protein sequencing via Edman chemistry, 2-D gels, and database searching:
• LC-TANDEM MS: MICRO/ NANO HPLC/ UPLC (EKSIGENT, WATERS); MS VIA TOF, QTOF (WATERS)
AND ION TRAP MS (ORBITRAP, LTQ-XL/ETD, THERMO)
• MALDI-TANDEM MS: ABI 4800+ TOF/TOF (ABI-SCIEX); VOYAGER (PERSEPTIVE BIOSYSTEMS)
• ROBOTICS/ SAMPLE HANDLING: ADVION NANOMATE; SYMBIOT/ TECAN PLATE SPOTTERS; CEM
MICROWAVE
• BIOINFORMATICS: MASCOT (SYSADMIN FOR MASCOT CLUSTER INSTALLATION); LIMS; PERL SCRIPTING
• PROTEOMICS TECHNOLOGIES: 1D/ 2D GELS (IPGPHOR)
SELECTED PUBLICATIONS
(full list of publications and presentations available on request)
1. Neculai D, Neculai AM, Verrier S, Straub K, Klumpp K, Pfitzner E, Becker S., “Structure of the Unphosphorylated STAT5a
Dimer", J. Biol. Chem, 280 (49), 40782-7 (2005).
2. R. Henningsen, B. Gale, K. Straub, and D.C. Denagel, Application of Zwitterionic Detergents to the Solubilization of
Integral Membrane Proteins for Two-Dimensional Gel Electrophoresis and Mass Spectrometry", Proteomics, 2, 1479-1488
(2002).
3. Zhang HZ, Rao K, Carr SF, Papp E, Straub K, Wu JC, Fried J., “Rationally Designed Inhibitors of Inosine Monophosphate
Dehydrogenase", J. Med. Chem, 40 (1), 4-8 (1997).
4. J. O. Link and K. Straub,"Trapping of an IMP Dehydrogenase-Substrate Covalent Intermediate by Mycophenolic Acid", J.
Amer. Chem. Soc., 118, 2091-2092 (1996).
5. J. Nakamura, K. Straub, J. Wu, and L. Lou,"The Glutamine Hydrolysis Function of Human GMP Synthetase", J. Biol.
Chem., 270, 23450-23455 (1995).
6. L. Lou, J. Nakamura, S. Tsing, B. Nguyen, J. Chow, K. Straub, H. Chan, and J. Barnett, "High-Level Production from a
Baculovirus Expression System and Biochemical Characterization of Human GMP Synthetase", Protein Express. Purif., 6,
487-489 (1995).
7. Q.-F. Gan, G.L. Witkop, D.L. Sloane, K.M. Straub, and E. Segal, “Identification of a Specific Methionine in Mammalian 15-
Lipoxygenase which is Oxygenated by the Enzyme Product 13-HPODE: Dissociation of Sulfoxide Formation from Self-
Inactivation”, Biochemistry, 34, 7069-7079 (1995).
8. K. Straub and M. Levy, “Charaterization of Hapten Binding to Immunoconjugates by Electrospray Ionization Mass
Spectrometry”, Bioconjugate Chem., 5, 194-198 (1994).
9. L.C. Antonino, K. Straub and J. Wu, “Probing the Active Site of Human IMP Dehydrogenase Using Halogenated Purine
Riboside 5’-Monophosphates and Covalent Modification Reagents”, Biochemistry, 33, 1760-1765 (1994).
10. J. Barnett, K. Straub, B. Nguyen, J. Chow, R. Suttman, K. Thompson, S. Tsing, P. Benton, R. Schatzman, M. Chen, and H.
Chan, “Production, Purification, and Characterization of Human Matrilysin (PUMP) from Recombinant Chinese Hamster
Ovary Cells”, Protein Expres. Purific. 5, 27-36 (1994).
41.