Quality control of herbal medicines aims to ensure their consistency, safety and efficacy. Chemical fingerprinting has been demonstrated to be a powerful technique for the quality control of herbal medicines. Crude drugs are extracted with solvents in their increasing order of polarity (from non-polar to polar), In this procedure, the active constituents, which are soluble with specific range of polarity, get extracted in the solvent and the remaining marc is further extracted with the following solvent.

1. Quality Control & Standardization Of
Herbals
Evaluation Of Commercial Crude Drug Intended For
Use
By
Kaushal Kumar

2. TABLE OF CONTENTS
01 02 03 04
Introduction Evaluation Chemical
Evaluation
Biological
Evaluation

3. Quality control of herbal medicines aims
to ensure their consistency, safety and
efficacy.
Chemical fingerprinting has been
demonstrated to be a powerful technique
for the quality control of herbal medicines.
Introduction

4. Method of drug evaluation :
Organoleptic Evaluation
Physical Evaluation
Microscopical Evaluation
Chemical Evaluation
Biological Evaluation
Analytical Evaluation
Crude drugs are extracted with solvents in their increasing order of polarity (from non-
polar to polar), In this procedure, the active constituents, which are soluble with specific
range of polarity, get extracted in the solvent and the remaining marc is further extracted
with the following solvent.
Method

5. Chemical Evaluation
Alkaloids Description
Mayer test :
Little quantity of filtrate is dissolve with
few drop of Mayer’s reagent.
Yellow to off white ppt
Wagner’s Test :
Little quantity of filtrate is dissolve with
few drop of Wagner's reagent.
Reddish-brown ppt
Dragandroff’s Test :
Little quantity of filtrate is dissolve with
Dragandroff’s reagents.
Red color ppt.
Hager’s Test :
Little quantity of filtrate is dissolve with
Hager’s reagents
Yellow ppt.

6. Chemical Evaluation
Glycoside Description
Legal’s test :
Treat the extract with pyridine, methanolic
alkali & sodium nitroprussides.
Pink to Red color
Bal jet Test :
Treat the test extract with sodium picrate.
Orange-yellow color
Froth Test :
Add distilled water to the test extract and
shake.
1 c.m of foam develop
Liebermann-Burchard’s Test :
Add chloroform to the test extract , Filter, add drop of
acetic anhydride & concentrated h2so4through the
walls of the test tube.
Brown rings develops the junction of
two layer.

7. Chemical Evaluation
Phenolic compound & Tannins
Ferric chloride test :
Add ferric chloride solution (5%) to the test extract.
Blue-Black
color
Lead acetate Test :
Add 10% lead acetate solution to the test extract.
Yellow ppt
Alkaline Reagent Test :
Add NaOH solution to the test extract.
Yellow color
Shinoda Test :
Add a few fragments of Mg metal to the test extracts then add conc.
HCL
Magenta color
Gelatin Test :
Add gelatin solution (1%) containing NaCl to the test extract.
White ppt

8. Chemical Evaluation
Carbohydrates & Proteins
Molish test :
Add alcoholic ∝ napthol solution & con. H2sO4 to the test extract.
Violet ring at
the junction.
Benedict's Test :
Add Benedict's reagent to the test extract, Heat mixture on water
bath.
Orange-red
ppt
Fehling’s Test :
Add dilute HCL to the test extract and heat. Add equal vol. of Fehling
A & B
Deep Red
color
Millon’s Test :
Add Millon’s reagent to the test extract.
White ppt turn
red on heating
Ninhydrin Test :
Add ninhydrin reagent to the test extract and boil.
Blue color

9. Biological Evaluation
TLC
HPTLC
GC
HPLC
CC
Analytical Evaluation
Antidiabetic activity
Anti-inflammatory
activity
Anti-ulcer activity
Biological Evaluation

10. THANKS!
Do you have any questions?
easyconceptkaushal@gmail.com
+91 9546688666