1. Automation and Robotics: Developing and programing methods for
different liquid handling platforms such as STAR , STARlet, Nimbus,
ATOLL, Phynexus, TECAN.
Protein chemistry: Optimization of methods for the purification of
recombinant antibodies, glycoprotein, bispecific constructs, Fc fusion
proteins, antigens and affinity-tagged (6x His-tag) proteins from E. coli
and Mammalian cells using affinity (Protein A, Protein G and IMAC) and
preparative SEC methods (AKTA Purifier), spectrophotometric
quantitation of protein samples
Protein Spectroscopic analysis, SDS-PAGE, IEF, Western Blotting, Size
Exclusion Chromatography, high performance liquid chromatography
(HPLC), Differential Scanning Calorimetery (DSC), ELISA, dissolution,
Activity and Cell based assays, DLS, capillary electrophoresis.
Molecular biology: standard DNA manipulation methods including
conventional cloning (restriction enzyme digestion, agarose gel
electrophoresis, gel purification and ligation of DNA fragments, bacterial
transformation), ligation independent cloning (Novagen), construction
of expression vectors, maxipreps, minipreps, colony PCR, PCR, DNA
sequencing (Applied BioSystems 3100 sequencer) and quantitation
(Nanodrop and Labchip DS).
Bioinformatics : Systematic training in Computation Science and
Bioinformatics. Proficient in using various Bioinformatics software and
software development by using Python, Java, Perl, Google App Script.