4a. Biobased crops - Anker Sørensen

1. Flexilis® - The rubber dandelion Crop
Anker P Sørensen
VP New Business KeyGene
The crop innovation company KIT, Amsterdam, April 4 2017

2. The crop innovation company 2
The Challenge
Imagine you want to develop a novel crop!

3. The crop innovation company 3
The Challenge
Where is the a roadmap?
Where is the roadmap
collaboration
development
business plan Business model
value chain finance Scale up
germplasm research tools research
IP

4. The crop innovation company 4
Alternative Natural Rubber source
New Crop for temperate regions of the world
Natural Rubber Market :22.9 billion $ per year (2012)
Hevea brasiliensis is the only source
Production is threatened by:
diseases
oil palm replacement
lack of cheap, skilled tappers
unwanted deforestation
Natural rubber cannot be replaced by synthetic rubber in many applications
9
2
1
1Consumer
Industrial
Medical
Automotive
Segment
NR demand,
2015 (Mt)


5. The crop innovation company 5
Alternative Natural Rubber source
New Crop for temperate regions of the world
Nicolai I. Vavilov
(1887-1943)
Taraxacum Koksaghyz
discovered in 1931
750 Kg High
Quality Natural
Rubber per ha.
per year.
Taraxacum Koksaghyz
gown 1945-1955
Target Yield 2020

6. The crop innovation company 6
Alternative Natural Rubber source
New Crop for temperate regions of the world
TKS = Taraxacum “Koksaghyz” from Gene Banks
TO = Taraxacum Officinale
TO TKS
Collecting TKS in Kazakhstan


7. The crop innovation company 7
Alternative Natural Rubber Crop
Research Strategy – how do we get fro 200 -750 Kg
VIGOUR genes
RUBBER genes
mutation
mutation
Molecular breeding
Common dandelion, TO
2x = 16
Sexual, outcrosser
825 Mb
Kazakhstan dandelion, TKS, 200 Kg NR / ha
2x = 16
Sexual, outcrosser
1250 Mb

8. The crop innovation company 8
Alternative Natural Rubber Crop
Molecular Breeding Tools & Perseverance
RTA
CPT
TO75TKS25
TO100
X
TO100
X TO50TKS50
TO75TKS25
TKS100
X
TKS100
X
TKS100XTO37TKS63
TO75TKS25
TO10TKS90
G6
P generation
F1
G2
G3
G4
TKS100
XTO19TKS81
G5
E37/M43_188.39
E50/M37_323.65
E43/M33_234.24
0.0
E50/M38_529.430.7
E50/M34_289.882.8
E43/M41_199.004.6
E43/M41_198.14
E50/M32_061.70
E50/M37_110.95
5.8
E55/M35_246.738.5
E43/M33_356.06
E37/M35_099.96
11.5
qRD_a13.8
qMW_a
TC13_L
E43/M33_493.25
E43/M36_095.17
17.7
C29_L20.4
E50/M32_404.1023.6
E50/M37_314.0027.0
E50/M38_106.55
E37/M31_305.39
E43/M34_168.29
28.9
qRwn_a29.3
E43/M41_223.0833.1
E43/M41_502.7635.0
E37/M34_076.8940.4
E50/M32_395.6343.7
E43/M40_405.3245.7
E50/M45_268.5449.1
E55/M35_163.05
E50/M38_068.88
50.0
E43/M40_092.48
E43/M36_091.2850.5
E43/M36_155.9550.9
E43/M41_106.4855.0
E50/M42_622.9256.1
E43/M33_322.0757.8
TC49_L59.5
E43/M45_352.44
E55/M35_107.3061.0
E43/M41_052.2863.0
TC28A_L72.3
E37/M34_541.49
E50/M32_502.46
E37/M43_118.27
75.2
qRD_b77.6
E50/M42_359.1577.7
E43/M41_120.9388.6
E37/M43_068.9090.6
Lg:I
E37/M35_225.070.0
E43/M34_296.644.7
E43/M41_220.92
E43/M41_216.90
E50/M38_201.52
8.2
E43/M36_097.7410.5
E55/M35_160.0215.3
qFR_a16.3
E50/M40_096.03
E43/M33_056.8616.4
E37/M34_207.3020.8
qRL_b22.3
qLS_c25.8
E43/M33_169.5725.9
E37/M31_518.6126.9
E50/M42_198.8327.5
E50/M34_083.1531.3
E43/M33_402.6832.0
E50/M34_203.2733.5
qMW_b33.9
E43/M33_107.55
E50/M34_140.1335.1
qRD_d
E50/M42_524.7436.7
E50/M42_148.4238.7
E43/M45_268.6640.2
E50/M34_191.3942.4
E50/M34_354.7243.1
E50/M40_188.7643.5
E50/M44_277.6444.6
qSR_c
E50/M37_312.2345.0
E50/M34_213.9045.5
E50/M32_199.3346.0
E43/M41_202.3748.0
E50/M34_153.89
E37/M34_145.9248.3
E43/M36_055.5848.8
qLR_b50.3
E37/M31_365.8453.1
E43/M40_057.92
E37/M43_343.9059.5
E43/M41_435.8671.0
E50/M37_107.9772.3
E37/M31_279.2882.1
E50/M32_291.3383.0
E43/M41_105.5286.5
E37/M31_281.3491.8
E55/M35_234.51103.5
E43/M45_195.70116.1
Lg:II
E43/M33_118.10
E37/M43_243.820.0
qRL_c
E43/M33_271.552.7
E43/M41_106.91
E50/M40_076.65
E43/M34_121.01
3.4
E37/M31_114.197.5
E50/M37_469.84
E50/M42_055.25
E50/M44_068.69
10.9
E50/M34_454.70
E50/M40_553.3415.0
E50/M40_088.8916.3
E43/M34_139.71
E43/M34_163.24
E50/M38_295.23
17.0
E43/M33_331.0020.1
E55/M35_091.3820.7
E50/M38_121.6527.6
E37/M43_405.1628.2
E37/M31_085.2432.2
E50/M44_099.2132.7
E50/M44_098.2733.8
E50/M42_048.07
E37/M31_455.3936.8
E37/M34_454.53
E50/M32_467.59
E43/M40_173.40
39.1
E43/M41_376.60
E43/M45_283.22
E43/M33_166.27
39.5
E50/M40_141.33
D0540.0
E50/M46_168.5640.6
E50/M38_057.47
E37/M35_561.8541.2
E50/M44_187.0345.4
E43/M45_243.3846.4
E50/M34_171.2450.4
qLS_d
E43/M36_399.0855.2
E43/M41_378.9856.6
E50/M45_305.3658.0
E43/M33_241.57
E55/M35_127.64
E50/M40_310.15
59.8
E50/M32_144.65
E50/M42_195.9961.9
E55/M35_180.2963.5
E50/M37_096.1372.5
E50/M37_168.8674.0
E50/M44_130.8474.7
E50/M37_380.2577.0
E50/M37_055.14
E50/M38_255.7881.0
E50/M44_152.2184.0
E50/M32_157.7284.4
qLS_a86.5
E37/M31_301.4586.6
qSD_a
qTD_a
E43/M33_167.33
91.4
E43/M41_260.0092.2
E43/M40_265.1895.9
qLV_a
E37/M43_086.00
99.3
E43/M36_143.53
E43/M41_302.49
E43/M41_444.36
99.6
A27103.3
E43/M41_266.11106.4
E37/M31_143.97108.4
qFR_b112.5
E50/M42_402.96112.6
E50/M37_064.58113.7
E43/M33_095.00119.7
E50/M40_151.09120.5
E37/M43_233.00121.4
Lg:III
E43/M33_077.210.0
E50/M37_160.0011.8
E43/M41_229.98
TC28B12.8
E50/M37_149.30
E43/M33_178.87
15.5
E43/M41_111.7917.3
E37/M31_451.3118.1
E43/M41_188.2219.0
E43/M33_110.7021.7
E43/M40_124.4222.6
E43/M40_126.25
E50/M44_161.95
qRwn_b
23.3
E55/M35_099.9227.9
E50/M37_134.4129.3
E43/M34_141.4332.5
E43/M33_122.6332.7
E50/M32_087.1336.0
E43/M36_572.16
E43/M36_109.77
E55/M35_060.44
38.4
E50/M42_192.3040.9
E43/M45_210.86
E50/M37_140.56
E43/M36_176.89
E50/M34_415.81
SML038
41.0
E50/M34_106.7341.8
E37/M31_210.22
E43/M34_190.8142.0
E50/M42_260.3343.3
E43/M41_144.4044.0
E43/M34_116.5547.2
E55/M35_235.6148.1
E50/M45_265.9048.4
E43/M41_424.3450.5
TC16_L
E50/M42_117.24
52.6
E43/M36_317.4254.7
qLS_e59.0
E50/M32_316.1259.1
E43/M33_312.7560.2
TC4160.7
E43/M33_416.3070.6
qLS_b71.3
E43/M33_164.38
qRD_c
E43/M41_294.02
75.2
E43/M40_220.4175.5
E37/M31_124.7376.3
E43/M34_126.2981.2
E50/M40_063.86
E43/M34_266.08
T22_L
83.8
qLV_b84.7
E50/M34_339.6188.2
E50/M34_337.3189.0
E50/M46_267.4792.5
E50/M42_239.4493.8
E50/M32_162.89
E50/M32_175.9193.9
E50/M32_289.31
E50/M37_492.3396.4
E50/M40_142.2398.1
qLS_f100.8
E50/M44_075.87101.1
E37/M43_306.63
E50/M32_532.48104.5
qMW_c105.8
E43/M33_098.51112.3
E50/M46_192.06112.6
E43/M40_072.04116.7
E50/M40_283.65
E37/M34_319.39
E50/M45_218.84
116.8
E43/M45_323.73118.5
E50/M45_100.24
B12_L122.2
E50/M32_060.02124.0
TC38_L128.1
E50/M32_304.07130.9
Lg:IV
E43/M41_055.790.0
E50/M34_226.972.6
E43/M41_123.706.6
TC319.6
E37/M35_200.519.9
E50/M40_113.87
E37/M35_103.25
E50/M44_439.14
12.5
E43/M34_281.7013.9
E50/M40_109.1515.7
E43/M40_516.9716.7
E50/M34_278.35
E43/M36_146.90
19.2
E50/M42_355.8021.3
E43/M45_364.0921.8
E43/M33_347.4125.6
TC42_L26.6
qSR_d
E55/M35_059.81
E37/M34_388.01
27.6
E43/M41_073.3029.2
E50/M32_446.0430.2
E50/M45_294.3539.2
E37/M43_223.7641.0
E43/M33_198.9541.9
E37/M35_484.78
E50/M44_199.2042.0
E50/M42_052.4745.3
E37/M43_198.62
E50/M45_112.6746.2
E37/M43_251.2746.3
E43/M36_246.2948.4
E50/M32_407.99
E50/M45_220.8148.9
E50/M38_237.5150.6
E50/M42_319.8250.7
E55/M35_181.63
TC8B51.5
E50/M44_082.81
E50/M34_249.3854.3
E50/M38_248.5955.2
E43/M36_127.5256.1
E37/M43_076.2057.1
E50/M44_270.3359.5
E37/M31_223.1661.9
E37/M31_237.0362.5
E50/M32_191.03
E50/M34_076.40
66.2
E43/M40_060.4067.8
qSR_a68.5
E37/M35_150.9068.6
E37/M34_065.6577.3
E50/M32_225.6080.2
E37/M43_467.3780.3
E50/M46_116.6082.8
E37/M35_280.18
E43/M36_077.3884.4
E43/M40_163.20
E50/M42_323.5585.1
E43/M36_051.9386.7
E37/M35_080.0187.5
TC4590.5
qSD_b
E43/M36_184.0894.4
TC2697.7
E50/M42_176.3199.7
E43/M36_181.80101.4
E43/M36_168.61
E43/M36_189.76101.5
E43/M36_306.41106.4
E50/M37_086.51
E43/M36_125.98
E50/M40_265.12
108.1
E43/M45_112.89109.9
E50/M44_109.21110.8
Lg:V
E50/M45_098.820.0
E50/M34_298.881.8
E50/M40_067.272.0
E50/M45_210.493.5
E55/M35_334.024.0
(TC46_L)10.0
E43/M36_067.50
E43/M34_524.27
E50/M38_216.73
12.9
E37/M35_160.9915.2
E50/M44_119.2319.4
E37/M34_448.1520.5
E55/M35_288.8921.5
qRL_a21.9
E50/M34_094.49
E50/M42_276.7924.6
E37/M35_429.5625.5
C0627.3
E43/M36_271.8329.3
E43/M41_127.3329.8
E50/M34_242.83
E50/M38_105.0830.3
E37/M34_090.67
E37/M35_217.1230.7
E50/M32_418.4432.7
E50/M37_361.9633.5
E43/M40_199.0042.6
E50/M38_086.7445.8
E55/M35_063.90
E37/M43_519.6646.9
E50/M42_221.83
E55/M35_254.4647.8
E43/M36_406.0148.8
E43/M36_345.58
E37/M34_068.89
E43/M33_341.83
50.5
E50/M44_248.6651.1
qCC_a
E50/M32_376.68
E50/M37_215.23
E50/M42_588.12
E55/M35_209.93
52.4
E43/M34_351.3254.0
E50/M44_345.7955.4
E55/M35_481.7960.5
qSR_b66.6
E55/M35_120.0866.7
E43/M41_058.4368.6
E37/M31_176.8369.6
E50/M42_475.37
E43/M36_051.0773.7
qLR_a81.6
E43/M45_121.6181.7
E50/M32_063.9184.2
E43/M45_182.6487.4
E50/M46_198.9797.1
E50/M32_311.48
E55/M35_271.8798.3
E43/M41_122.39
E50/M32_158.83101.7
E50/M40_459.63106.9
E50/M34_280.64109.4
E50/M40_288.39110.6
E50/M44_173.30115.7
Lg:VI
E43/M41_525.120.0
E43/M34_273.6221.4
E43/M40_267.71
E43/M41_276.48
E50/M40_194.27
TC14
22.5
E50/M44_389.4323.4
E50/M37_556.6824.3
E43/M33_206.71
E55/M35_198.75
E43/M33_144.59
25.2
E37/M31_293.0226.0
E50/M46_566.8126.1
E50/M40_274.8626.9
E50/M32_571.9227.0
E43/M41_374.6928.6
E50/M44_536.5829.4
E43/M40_214.79
E37/M31_609.6429.5
E43/M34_165.1931.9
TC8A35.9
E50/M32_185.47
E43/M36_222.1036.5
TC6A37.0
E50/M38_184.7138.1
E50/M37_109.4639.9
E55/M35_268.71
E43/M45_310.3943.6
E50/M38_134.9047.1
E37/M31_109.4549.1
E37/M43_226.5551.0
E43/M40_074.5653.5
E50/M45_560.2054.5
TC5059.0
E43/M34_132.8460.1
E50/M44_097.7163.0
E43/M34_196.6668.3
TC27L69.1
E43/M34_384.50
E43/M33_127.45
E43/M33_126.45
72.8
E43/M41_076.3974.0
E43/M33_185.5078.8
E37/M43_097.98
E43/M34_354.03
E50/M34_210.74
E50/M42_206.78
80.2
E50/M37_127.2082.0
E43/M34_149.9485.1
E50/M40_254.5786.7
Lg:VII
E37/M34_102.360.0
E43/M34_291.83
E43/M40_109.114.1
E43/M41_491.406.8
E43/M36_369.087.1
E50/M40_164.668.0
E50/M40_352.758.6
E43/M41_515.69
E43/M34_201.598.7
E55/M35_197.0812.8
E43/M34_143.2013.8
E43/M34_131.2814.9
E50/M34_548.7215.0
E50/M40_078.8716.0
E50/M38_206.7619.1
E55/M35_264.2820.6
E50/M45_153.3621.1
E43/M40_062.8521.7
E37/M31_322.53
qSD_c
E37/M31_423.38
22.9
E50/M45_302.2724.2
qFR_c25.2
E37/M34_181.69
E37/M34_091.66
E37/M34_294.57
E50/M32_179.76
E50/M34_462.81
25.3
E50/M42_519.3826.0
E50/M46_170.4526.2
E50/M45_154.4127.2
TC19
E37/M43_156.7527.6
E43/M45_245.1529.4
E50/M45_080.1533.1
qTD_b34.6
qRD_e36.7
E50/M32_092.4237.0
E43/M41_120.3638.5
E55/M35_124.9041.6
E43/M40_135.9344.6
E50/M32_438.60
E43/M36_523.0745.6
E43/M36_543.0646.4
A1946.8
qLV_c47.9
E50/M45_173.88
E37/M35_127.25
51.8
TC6B54.8
E50/M42_169.24
E43/M45_381.5755.9
E43/M34_216.3159.3
qRL_d
E37/M34_223.6659.9
E50/M32_521.5562.6
E55/M35_560.5466.3
E50/M44_303.1166.4
E50/M37_065.70
E50/M44_292.07
E37/M43_120.92
70.6
E43/M41_064.4573.8
E37/M31_112.1375.1
E55/M35_203.6476.8
E37/M35_062.7080.5
Lg:VIII

9. The crop innovation company 9
Alternative Natural Rubber Crop
IP
Product Trademark Process patent
PBR on varieties

10. The crop innovation company 10
Alternative Natural Rubber Crop
Results Yield
Selection result over years
TKS x TO – novel crop TKS
2016 Yield Trial

11. The crop innovation company 11
Alternative Natural Rubber Crop
Collaboration & Partnership
2008
Launch
EU-Pearls project
2016
Feasibility study
business plan
Spring Associates
NR shows strongpositivedemand
growth
NR productionis stagnating and
long-termsupply is underpressure
Supply uncertainties resultin
increased NR price volatility
+5%
+3%
2020E
17
2014
12
2010
11
Globalnaturalrubberconsumption,
2010-20E (Mton)
10
+4%
+4%
2020E
15
2014
12
2010
Naturalrubberprices*,
2000-15(US cents/lb)
Globalnaturalrubberproduction,
2010-20E (Mton)
0
40
80
120
160
200
240
280
320
2000 2005 20152010
Low
prices
Price
recovery
High
volatility
Pilot
2016 – 19
Pre-
commercial
2018 – 20
Commercial
2020
onwards
2016 2017 2018 2019 2020
Investigate extraction methods
Develop Business Plan
Preparation & support
Cultivate and harvest 50 ha
Second
sales
End 2020
First
sales
End 2018
Upscale production plant
Extract and sell second NR batch ([XX] ton)
Milestones
Select final extraction partner
Technological development
Prepare for commercial production
Activities
Prepare for scale up
Build pilot plant
Identify potential extraction partners
Submit subsidy application(s)
Pilot phase
Define (potential) structure NewCo
Extract and sell first NR batch ([XX] ton)
Cultivate and harvest 25 ha
Identify and select seed breeding partner
Pre-commercial phase
NewCo
structure
End 16
Business
Plan
Jun 16
TBD TBD
2014
Long-term
strategic
partnership KZ
Launch Drive4EU
project
2014
Collaboration
with Kultevat
(US)
2013
Organization
Wageningen UR
Institute of Batony
Rusthoeve
InExCB-KZ**
Agri. & Fisheries R.
Inst.
GEA westfalia
seperator
Netzsch
QEW engineered
rubber
Mitas
Apollo Vredestein
Tereos Syral
Joanneum Research
Work package
Project leader
Extraction
Extraction
Manufacturing
Manufacturing
Manufacturing
Agronomy
Agronomy
Agronomy
R&D, breeding
Extraction
Exploitation
Country

12. The crop innovation company 12
Alternative Natural Rubber Crop
business plan & value chain
End
client
Use of
end-
product
~70% of NR is sold directly to tyre manufacturing
companies*
Extractor &
Trader
Produces and
sells NR
Compounder
Compounds &
vulcanizes NR
grades
Moulder/
Wholesaler
Moulds NR to
(semi-)finished
products
End-product
manufacturer
Processes
moulds into
end products
Upstream Downstream
Seed
producer
Cultivates &
sells seeds
R&D
Breeding
?
Farmer
Cultivates &
harvests NR

13. The crop innovation company 13
Alternative Natural Rubber Crop
Scale up and phasing
Milestones
Technology
development
Commercial
development
 Investigate extraction method
for large-scale production
 Investigate scale up seed
production
 Increase production area
towards commercial scale
 Further develop crop & yield
 Increase production area
 Further develop crop & yield
 Build extraction plant
Pilot
Pre-commercial
Commercial
First sales Commercial launch
2016
2018
 Develop business plan &
feasibility study
 Identify strategic partnerships
 Attract launching end-client(s)
 Attract financing partners
 Attract additional licensing
partner(s)
 Prepare for commercial scale
production
 Assess sales of by-products
 Expand customer base based
on potential of external sales
 Penetrate into additional
market segments
 Launch by-product sales
Time to market
2020
Business plan

14. The crop innovation company 14
Alternative Natural Rubber Crop
Seed production, scale up & applications

15. The crop innovation company 15
Alternative Natural Rubber Crop
Summary
 A low-calorie
sweetener that is
currently produced
from chicory
 Remaining parts of
the plant, which can
be used as a source
of energy
TO
Flexilis
Hybrid
TKS
Molecular
breeding
Dry weight
(% of total)
 Latex, is directly
processed into dry
rubber during
extraction
Description
5-15%
50-60%
30-40%
Product
X 2.6
4.1
0.8
Target
Yield
(t/ ha)
 Processed foods
 Fuel (as ethanol)
 Bioplastic building blocks
 Feed
 Biogas
 Soil amendment
 Hardboard overlay
 Footwear
 Sports goods
 Airplane tyres
 Tubes & hoses
 Bridge bearings
Example
applications
Inulin
Biomass
NR

16. The crop innovation company 16
Goals and learnings?
Where is the a roadmap?
Where is the roadmap
Developed a new crop & a novel source of NR!
Established valuable partnerships.
Steered developments based on a sound business plan.
Developed efficient collaborations across the entire value
chain.
Demonstrated the value of genomics assisted breeding for
domestication of a novel crop.
collaboration
development
business plan Business model
value chain finance Scale up
germplasm
research tools research
partnership