Preparation of reagents , for Qualitative analysis of salts ,MANIK Qualitative analysis of metal ions and acid radicals: Na+, K+, Ca+2, Ag+, Mn+4, Fe+2, Fe+3, Co+2, Mg+2, Al+3, Cu+2 and acid radicals CO3, halides, Citrate SO4-2, NO3-, SO3-2, etc.

1. Sl.
No.
Name of the
Reagent
Purpose
(Exp.
No.)
Reagent(s)
to be used
(Name/Code)
Preparation Procedure &
Calculation
Shelf life of
prepared
reagent
Instructions to the Lab
Demonstrator & students
Md. Imran Nur Manik [manikrupharmacy@gmail.com] 2114:InorganicPharmacy-IILab Page 1
1
Salicylic acid
Benzoic acid
Na2CO3
NaOH
1
 Salicylic acid
 Benzoic acid
 Na2CO3
 NaOH
ONLY SOLID REAGENTS ARE REQUIRED
N/A N/A
2
Na2CO3
Potash Alum
2
 Na2CO3
 Potash Alum
ONLY SOLID REAGENTS ARE REQUIRED N/A N/A
3
Dilute (6M)
Hydrochloric acid
solution
[6M HCl]
3-A, 3-B
3-C, 3-D
 Hydrochloric
acid (HCl)
[Purity= 37%
& Density 1.19g/mL]
M.Wt.= 36.5 gm
 Distilled Water
(DW)
For the preparation of 250mL solution
1. Take 124.35 mL of HCl (Purity 37% and
Density1.19 gm/mL) with a measuring
cylinder in a 250mL volumetric flask.
2. Add about 30-40 mL DW.
3. Dissolve the acid. Use sonication if
necessary.
4. Adjust the volume to 25mL by
adding distilled water Q.S.
3 months
from the date
of
preparation.
 To be freshly prepared
(As far as possible)
 Be cautious when handle
37% HCl
 Safety goggles & Hand
gloves must be used while
handling HCl.
 Follow the labeling
instructions listed below.
4
0.1M Barium
chloride
solution
[0.1M BaCl2]
3-A
Barium chloride
BaCl2.2H2O
(Purity 99%)
M.Wt.= 244.28 gm
Distilled Water
(DW)
For the preparation of 50mL solution
1. Accurately weigh 1.2337 gm of BaCl2
(99% pure) in an electronic balance.
2. Take the solid in a 50mL volumetric flask.
3. Add about 10-15mL DW.
4. Completely dissolve the solids.
5. Adjust the volume to 50mL mark by
3 months
from the date
of
preparation.
 To be freshly prepared
(As far as possible)
 The weighing must
be accurate.
 Hand gloves must be used
while handling
BaCl2. 2H2O

2. Sl.
No.
Name of the
Reagent
Purpose
(Exp.
No.)
Reagent(s)
to be used
(Name/Code)
Preparation Procedure &
Calculation
Shelf life of
prepared
reagent
Instructions to the Lab
Demonstrator & students
Md. Imran Nur Manik [manikrupharmacy@gmail.com] 2114:InorganicPharmacy-IILab Page 2
adding distilled water Q.S.  Follow the labeling
instructions listed below.
5
Dilute (2M)
Nitric Acid
solution
[2M HNO3]
3-A
Nitric Acid
[HNO3] (Purity 65%
& Density 1.41g/mL)
M.Wt.= 63.01 gm
Distilled Water
(DW)
For the preparation of 100mL solution
1. Take 13.750 mL of HNO3 (Purity 65% and
Density1.41g/mL) with a pipette in a 100 mL
volumetric flask.
2. Add about 13-14mL DW.
3. Dissolve the acid. Use sonication if
necessary.
4. Adjust the volume to 100 mL by
adding distilled water Q.S.
3 months
from the date
of
preparation.
 To be freshly prepared
(As far as possible)
 Be cautious when handle
65% HNO3
 Safety goggles & Hand
gloves must be used while
handling HNO3
 Follow the labeling
instructions listed below.
6
Conc. (6M)
Nitric Acid
solution
[6M HNO3]
3-B
Nitric Acid
[HNO3] (Purity 65%
& Density 1.41g/mL)
M.Wt.= 63.01 gm
Distilled Water
(DW)
For the preparation of 100mL solution
1. Take 41.250 mL of HNO3 (Purity 65% and
Density1.41g/mL) with a measuring cylinder
in a 100 mL volumetric flask.
2. Add about 15-20 mL DW.
3. Dissolve the acid. Use sonication if
necessary.
4. Adjust the volume to 25mL by
adding distilled water Q.S.
3 months
from the date
of
preparation.
 To be freshly prepared
(As far as possible)
 Be cautious when handle
65% HNO3
 Safety goggles, Hand gloves,
Safety masks must be used
while handling HNO3
 Follow the labeling
instructions listed below.
Reagent bottles must be labeled with:

3. Sl.
No.
Name of the
Reagent
Purpose
(Exp.
No.)
Reagent(s)
to be used
(Name/Code)
Preparation Procedure &
Calculation
Shelf life of
prepared
reagent
Instructions to the Lab
Demonstrator & students
Md. Imran Nur Manik [manikrupharmacy@gmail.com] 2114:InorganicPharmacy-IILab Page 3
 Full name(s) of the chemical(s). Do not use abbreviations as these can be ambiguous;
 Date of transfer or preparation;
 Full name of person who transferred or prepared the solution;
 If the container holds a solution or mixture, concentrations and solvents used (e.g., 1M hydrochloric acid;40% acetonitrile / 60% water;1 mg/ml
naphthalene in hexane) needs to be indicated.
7
5% Silver
nitrate (AgNO3)
[5% AgNO3]
3-A
Silver nitrate
(AgNO3) (99.5%)
M.Wt.= 169.87 gm
 Distilled Water
(DW)
For the preparation of 50mL solution
1. Accurately weigh 2.5125 gm of
AgNO3 (99.5% pure) in an electronic
balance.
2. Take the solid in a 50 mL volumetric flask.
3. Add about 5-10mL DW.
4. Dissolve the solids. Use sonication if
necessary.
5. Adjust the volume to 50mL mark by
adding distilled water Q.S.
3 months
from the date
of
preparation.
 To be freshly prepared
(As far as possible)
 The weighing must
be accurate.
 Safety goggles,
And Hand gloves
must be used while
handling AgNO3.
 Follow the labeling
instructions listed below.
8
Dilute (2M)
Ammonia (aq.)
solution.
NH3(aq.)
i.e. NH4OH
[2M NH4OH]
3-A, 3-B,
3-C, 3-D
 Ammonia (NH3)
(Purity 25% &
Density 0.91g/ml)
M.Wt.= 17.031 gm
 Distilled Water
(DW)
For the preparation of 250mL solution
1. Take 37.3626 mL of NH3 (Purity 25% and
Density0.91g/mL) with a pipette in a 250 mL
volumetric flask.
2. Add about 40-50mL DW.
3. Dissolve the acid. Use sonication if
necessary.
3 months
from the date
of
preparation.
 To be freshly prepared
(As far as possible)
 Be cautious when handle
25% NH3
 Safety goggles ,
Hand gloves and
Safety masks
must be used while

4. Sl.
No.
Name of the
Reagent
Purpose
(Exp.
No.)
Reagent(s)
to be used
(Name/Code)
Preparation Procedure &
Calculation
Shelf life of
prepared
reagent
Instructions to the Lab
Demonstrator & students
Md. Imran Nur Manik [manikrupharmacy@gmail.com] 2114:InorganicPharmacy-IILab Page 4
4. Adjust the volume to 250mL by
adding distilled water Q.S.
handling NH3
 Follow the labeling
instructions listed below
9
Conc. (9M)
Ammonia (aq.)
solution.
NH3(aq.)
i.e. NH4OH
[9M NH4OH]
3-A, 3-B,
3-C, 3-D
 Ammonia (NH3)
(Purity 25% &
Density 0.91g/ml)
M.Wt.= 17.031 gm
 Distilled Water
(DW)
For the preparation of 150mL solution
1. Take 101.036 mL of NH3 (Purity 25% and
Density0.91g/mL) with a pipette in a 200mL
measuring cylinder.
2. Add about 30-40mL DW.
3. Dissolve the acid. Use sonication if
necessary.
4. Adjust the volume to 25mL by
adding distilled water Q.S.
3 months
from the date
of
preparation.
 To be freshly prepared
(As far as possible)
 Be cautious when handle
25% NH3
 Safety goggles ,
Hand gloves and
Safety masks
must be used while
handling NH3
 Follow the labeling
instructions listed below
10
5% Ferrous
sulphate
solution (FeSO4)
3-A
 Ferrous sulphate
heptahydrate
(FeSO4.7H2O) (99.5%)
M.Wt.= 278.02 gm
For the preparation of 50mL solution
1. Accurately weigh 4.5954 gm of
FeSO4.7H2O (99.5% pure) in an
electronic balance.
2. Take the solid in a 50mL volumetric flask.
3. Add about 5-10mL DW.
4. Completely dissolve the solids. Use
3 months
from the date
of
preparation.
 To be freshly prepared
(As far as possible)
 The weighing must
be accurate.
 Do not left the container in
open air.
[Tightly pack after use]

5. Sl.
No.
Name of the
Reagent
Purpose
(Exp.
No.)
Reagent(s)
to be used
(Name/Code)
Preparation Procedure &
Calculation
Shelf life of
prepared
reagent
Instructions to the Lab
Demonstrator & students
Md. Imran Nur Manik [manikrupharmacy@gmail.com] 2114:InorganicPharmacy-IILab Page 5
[5% FeSO4]  Distilled Water
(DW)
sonication if necessary.
5. Adjust the volume to 25mL mark by
adding distilled water Q.S.
 Safety goggles,
and Hand gloves
must be used while
handling FeSO4.7H2O
 Follow the labeling
instructions listed below
11
0.2M Ferric
chloride (FeCl3)
solution
[0.2M FeCl3]
3-A
 Ferric chloride
(FeCl3) (98%)
M.Wt.= 162.21gm
 Distilled Water
(DW)
For the preparation of 50mL solution
1. Accurately Weigh 1.6552 gm of FeCl3
(99.5% pure) in an electronic balance.
2. Take the solid in a 50mL volumetric flask.
3. Add about 5-10mL DW.
4. Completely dissolve the solids. Use
sonication if necessary.
5. Adjust the volume to 50mL mark by
adding distilled water Q.S.
3 months
from the date
of
preparation.
 To be freshly prepared
(As far as possible)
 The weighing must
be accurate.
 Safety goggles,
and Hand gloves
must be used while
handling FeCl3
12
1M Potassium
chromate
(K2CrO4)
solution
[1M K2CrO4]
3-B
 Potassium
chromate
(K2CrO4) (99%)
M.Wt.= 194.20 gm
 Distilled Water
(DW)
For the preparation of 50mL solution
1. Accurately weigh 9.8080 gm of K2CrO4
(99% pure) in an electronic
balance.
2. Take the solid in a 50mL volumetric flask.
3. Add about 5-10mL DW.
4. Completely dissolve the solids. Use
sonication if necessary.
5. Adjust the volume to 50mL mark by
adding distilled water Q.S.
3 months
from the date
of
preparation.
 To be freshly prepared
(As far as possible)
 The weighing must
be accurate.
 Safety goggles,
and Hand gloves
must be used while
handling K2CrO4.
13
0.1M Stannous
chloride (SnCl2)
solution
3-B
 Stannous
chloride (SnCl2.2H2O)
(97%)
For the preparation of 50mL solution
1. Accurately weigh 1.16304 gm of
SnCl2.2H2O (97% pure) in an electronic
3 months
from the date
of
preparation.
 To be freshly prepared
(As far as possible)

6. Sl.
No.
Name of the
Reagent
Purpose
(Exp.
No.)
Reagent(s)
to be used
(Name/Code)
Preparation Procedure &
Calculation
Shelf life of
prepared
reagent
Instructions to the Lab
Demonstrator & students
Md. Imran Nur Manik [manikrupharmacy@gmail.com] 2114:InorganicPharmacy-IILab Page 6
[0.1M SnCl2]
M.Wt.= 225.63 gm
 Distilled Water
(DW)
balance.
2. Take the solid in a 50mL volumetric flask.
3. Add about 5-10mL DW.
4. Completely dissolve the solids. Use
sonication if necessary.
5. Adjust the volume to 50mL by
adding distilled water Q.S.
 The weighing must
be accurate.
 Safety goggles,
And Hand gloves
must be used while
handling SnCl2.2H2O
14
2M Sodium
Hydroxide
solution
[2M NaOH]
3-B, 3-C
 Sodium
Hydroxide (NaOH)
(98%)
M.Wt.= 40 gm
 Distilled Water
(DW)
For the preparation of 250mL solution
1 Accurately weigh 20.4081 gm of NaOH
(98% pure) in an electronic
balance.
2. Take the solid in a 250 mL volumetric
flask.
3. Add about 30-40mL DW.
4. Completely dissolve the solids.
Use sonication if necessary.
5. Adjust the volume to 250 mL mark by
adding distilled water Q.S.
3 months
from the date
of
preparation.
 To be freshly prepared
(As far as possible)
 The weighing must
be accurate.
 Do not left the container in
open air.
[Tightly pack after use]
 Safety goggles,
and Hand gloves
must be used while
handling NaOH.
15
10M Sodium
Hydroxide
solution
3-B, 3-C
 Sodium
Hydroxide (NaOH)
(98%)
For the preparation of 250mL solution
1 Accurately weigh 102.0408 gm of NaOH
(98% pure) in an electronic
balance.
3 months
from the date
of
preparation.
 To be freshly prepared
(As far as possible)
 The weighing must
be accurate.

7. Sl.
No.
Name of the
Reagent
Purpose
(Exp.
No.)
Reagent(s)
to be used
(Name/Code)
Preparation Procedure &
Calculation
Shelf life of
prepared
reagent
Instructions to the Lab
Demonstrator & students
Md. Imran Nur Manik [manikrupharmacy@gmail.com] 2114:InorganicPharmacy-IILab Page 7
[10M NaOH] M.Wt.= 40 gm
 Distilled Water
(DW)
2. Take the solid in a 250 mL volumetric
flask.
3. Add about 30-40mL DW.
4. Completely dissolve the solids.
Use sonication if necessary.
5. Adjust the volume to 250 mL mark by
adding distilled water Q.S.
 Do not left the container in
open air.
[Tightly pack after use]
 Safety goggles,
and Hand gloves
must be used while
handling NaOH.
16
Potassium
Ferricyanide
3-B
3-C, 3-D
Potassium
ferricyanide 99%)
(K3[Fe(CN)6])
For the preparation of 25mL solution
1. Weigh 1gm of K3[Fe(CN)6] /
K4[Fe(CN)6] / KSCN in an electronic
balance.
2. Take the solid reagents in three
25mL volumetric flasks.
3. Add about 3-5mL DW in
each flask.
4. Completely dissolve the solids.
5. Adjust the volume to 25 mL marks in
each flasks by adding distilled water Q.S.
To be freshly
prepared
 The weighing must
be accurate.
 Do not left the container in
open air.
[Tightly pack after use]
 Safety goggles,
and Hand gloves
must be used while
handling NaOH.
Potassium
Ferrocyanide
Potassium
ferricyanide (99%)
(K4[Fe(CN)6])
Potassium
Thocyanate
Potassium
thiocyanate (KSCN)
17 Conc. HCl
Potassium Iodide,
Conc. H2SO4
Ammonium chloride
3-A, 3-B
3-C, 3-D
HCl (37%)
Only the reagents are required
N/A N/A
18 KI
19 H2SO4(98%)
20 NH4Cl (99%)

8. Sl.
No.
Name of the
Reagent
Purpose
(Exp.
No.)
Reagent(s)
to be used
(Name/Code)
Preparation Procedure &
Calculation
Shelf life of
prepared
reagent
Instructions to the Lab
Demonstrator & students
Md. Imran Nur Manik [manikrupharmacy@gmail.com] 2114:InorganicPharmacy-IILab Page 8
Nessler's reagent
Litmus paper (red)
21
Ammonium
carbonate
[(NH4)2CO3 ]
5% solution
3-D
Ammonium
carbonate
CH6N2O2. CH5NO3
30% purity
For the preparation of 25mL solution
1. Accurately weigh 1.1666 gm of
(NH4)2CO3 (30% pure) in an electronic
balance.
4. Completely dissolve the solids 25 mL
volumetric flask. Use sonication if necessary.
5. Adjust the volume to 25mL by
adding distilled water Q.S.
3 months
from the date
of
preparation.
 To be freshly prepared
(As far as possible)
 The weighing must
be accurate.
 Safety goggles, and Hand
gloves must be used while
handling (NH4)2CO3
Instructions for labelingof the solutioncontainers
Reagent bottles must be labeled with:
 Full name(s) of the chemical(s). Do not use abbreviations as these can be ambiguous;
 Date of transfer or preparation;
 Full name of person who transferred or prepared the solution;
 If the container holds a solution or mixture, concentrations and solvents used (e.g., 1M hydrochloric acid;40% acetonitrile / 60% water;1 mg/ml naphthalene
in hexane).
Temporary and transfer containers
Containers into which chemicals are filled only for the duration of the experiment, such as beakers, flasks, or test tubes, are referred to as temporary or transfer
containers. At a minimum, they should be labeled with the name of the chemical. Common abbreviations whose meaning is clear to EVERYBODY in the lab are
acceptable. If an experiment continues overnight or for multiple days, the label should also contain the name of the user.
Containers holding non-hazardous substances such as water must also be labeled to avoidconfusion with other clear and colorless chemicals.
References
1. G.Svehla:Vogel's Qualitative Inorganic Analysis;7e;Pearson EducationIndia, 2008 .
2.A. J.Berry: Qualitative Inorganic Analysis;reprintof paperback edition (2015); Cambridge University Press, 2016

9. Sl.
No.
Name of the
Reagent
Purpose
(Exp.
No.)
Reagent(s)
to be used
(Name/Code)
Preparation Procedure &
Calculation
Shelf life of
prepared
reagent
Instructions to the Lab
Demonstrator & students
Md. Imran Nur Manik [manikrupharmacy@gmail.com] 2114:InorganicPharmacy-IILab Page 9
3. NRC(National Research Council). PrudentPractices in the Laboratory. Handling and Managementof Chemical Hazards. NationalAcademy Press, Washington, DC,
2011.
Preparedby
Course instructor
(Md. Imran Nur Manik)
Lecturer
Department of Pharmacy
Northern University Bangladesh