26. Aims of following safety rules and practices
• To prevent the occurrence of accidental
infections in the lab and reduce them if they
occur.
• Prevent potentially harmful microbes from
leaving the lab.
27. Safety in the Microbiology Lab
• Wash your hands with
soap and water before
you begin and after you
finish your work and
before leaving the lab.
28. Safety in the Microbiology Lab
• Decontaminate your
work surface before and
after you work
29. Safety in the Microbiology Lab
• No bags inside the lab.
30. Safety in the Microbiology Lab
• Wear only closed-toed
shoes in the laboratory.
31. Safety in the Microbiology Lab
• Wear lab scrub and
fully- buttoned lab coat.
32. Safety in the Microbiology Lab
• Tight your hair.
• No cosmetics
33. Safety in the Microbiology Lab
• If you are:
– Injured…
– Immunocompromised..
– Pregnant…
– Allergic to latix…
34. Safety in the Microbiology Lab
• Be careful not to burn
yourself when using
Bunsen burner.
35. Safety in the Microbiology Lab
• Keep your hands away
from your mouth and
eyes…
36. Safety in the Microbiology Lab
• Do not eat, drink or
chew gum while you are
in the lab
37. Safety in the Microbiology Lab
• Avoid using electronic
devices …
– Mobiles, MP3
players, laptops…
38. Safety in the Microbiology Lab
• Never mouth pipetting.
39. Safety in the Microbiology Lab
• Always keep cultures in
a test tube rack
whether you are
working at your bench
or walking about in the
lab.
40. Safety in the Microbiology Lab
• Place all contaminated
tubes, plates and waste
materials in appropriate
receptacles for
subsequent sterilization.
41. Safety in the Microbiology Lab
• If you spill a chemical on
your skin, immediately
wash the affected area. If a
chemical or a culture gets
into your eye, immediately
wash your eye for 5 min.
42. Safety in the Microbiology Lab
• If anything containing viable bacteria spills,
cover the spell or broken glass with a paper
towel and a generous amount of disinfectant
to remain on the slip for at least 20 min.
before cleaning it up.
43. Safety in the Microbiology Lab
• Sweep up pieces of broken glass (after
disinfection if necessary) and place them in
the specified container.
44. Safety in the Microbiology Lab
• Place sharps, such as
syringes and slides, in a
designated sharp
containers.
45. Safety in the Microbiology Lab
• Do not keep cultures in your lab drawer or
locker when you have finished with them.
• Do not remove cultures, reagents, or other
materials from the lab unless you have
permission to do so.
46. Safety in the Microbiology Lab
• Know the first aid kit location in your lab.
47. So, do we have to apply such
rules in the hospital?!
Yes, because…
48. You have to apply :
The Preventive
Measures
Personal
Procedural
Environmental
49. 1. Personal
• Hand hygiene.
• Fingernails, artificial nails and jewelry.
• Post exposure prophylaxis.
• Prevention of ventilator associated
pneumonia.
• Safe injection practices.
50. 1. Personal
• Hand hygiene:
(Resident flora and transient flora)
– Be sure to wash your hand before and
after the patient contact
– Be sure to wash your hand before starting
invasive procedures
– Soaps are detergent based products that have mini if
any antimicrobial activity.
– Alcohol based hand rubs have activity against broad
spectrum of medically important pathogens.
51. 1. Personal
• Fingernails, artificial nails and jewelry:
– The subungual area of the fingers harbor high
concentrations of bacteria both before and after
handwashing.
– Wearing at least one ring on the dominant hand
increases the number of microbes contaminating
the hand.
52. 1. Personal
• Post exposure prophylaxis:
• TB, HBV, HCV, HIV and influenza viruses
• Prevention of ventilator associated
pneumonia:
– arises when there is bacterial invasion of the
pulmonary parenchyma in a patient receiving
mechanical ventilation
– Eliminate contamination of equipment
– Practice hand hygiene prior to and following care
53. 1. Personal
• Safe injection practices:
– Unsafe injection practices have been the source of
many HCV outbreaks.
55. 3. Environmental
– House keeping practices for environmental
surfaces:
• Environmental surfaces (e.g., floors) are considered
noncritical surfaces requiring low-level disinfection.
When equipment and environmental surfaces are
visibly contaminated with blood, body fluids or other
potentially infectious materials, or are soiled with or
used in the presence of infectious materials,
decontamination should be performed immediately
following a procedure.
56. 3. Environmental
• Laundry:
– laundry which has been soiled with blood or other
potentially infectious materials . Contaminated
laundry shall be handled as little as
possible, placed and transported in labeled or
color-coded bags.
57. 3. Environmental
• Personal protective equipments:
– specialized clothing or equipment worn by an
employee for protection against a hazard
– Hand hygiene should be performed immediately
after the removal of any personal protective
equipment.
58. 3. Environmental
• contaminant, labeling, and disposal of
biohazard waste:
– Biohazardous waste includes liquid or semi-liquid
blood or other potentially infectious materials;
contaminated items that would release blood or
infectious materials in a liquid or semi-liquid state.
– All containers should be labeled or color-coded.
59. To Contact Me
• Register on FB:
Hibah’s microclasses
Note: please send your name, department
and Gp#
• e-mail: abusulaiman.hw1@gmail.com
