enzyme linked immunosorbent assey .elisa is one of the diagnosis biochemistry in serology. we have 4 classes in elisa : direct,indirect,sandwich,competitive.

1. ELISA
virology
Hamid Afkhami

2. EL Enzyme-Linked
Enzyme catalysis the substrate and produce colored
species.
IS ImmunoSorbent
Antigen or antibody are sticked in wells
About ELISA

3. Types of the ELISA
1. Direct
2. Indirect
3. Sandwich
4. competitive

4. Direct ELISA
 STEPS:
 Antigen stick to well with
charge + casein or
albumin(BSA).
 Add sample to well
 Wash
 Add substrate
 acid
 measure OD

5. Advantages
 Quick
 Eliminate Cross-reactivity of
secondary Ab
 Disadvantages
 the signal is less amplified
 Labeling has destructive effects
on ab and consuming time.
Direct ELISA

6.  STEPS:
 Antigen stick to well with
charge + casein or
albumin(BSA).
 Add sample + Wash well
 Add detector ab + wash
 Add substrate and measure
OD
Indirect ELISA

7.  Advantages:
 High sensitivity
 Flexibility
 This format of indirect ELISA is
suitable for determining total
antibody level in samples
 Cost saving
 Several Epitopes for secondary
Ab and signal amplification
Indirect ELISA

8.  Antigen detection
 STEPS:
 Stick ab to well + BSA
 Add sample (ag) + wash
 Add detector ab + wash
 Add substrate + measure OD
 Advantages
 High specificity
 Suitable for complex samples
SANDWICH ELISA
contain at least two
antigenic

10. COMPETITIVE ELISA

11. COMPETITIVE ELISA

12.  Advantages
 Suitable for complex samples
 Flexibility and sensitivity
COMPETITIVE ELISA

13.  http://www.elisa-antibody.com/ELISA-Introduction
 https://www.bio-rad-antibodies.com
 https://www.youtube.com
 http://arian-andish.com
 https://en.wikipedia.org/wiki/ELISA
Sources

14. Have a nice day