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Ionization techniques in lcms.ppt
1. Raghavendra Institute of Pharmaceutical Education and Research - Autonomous
K.R.Palli Cross, Chiyyedu, Anantapuramu, A. P- 515721
Ionization
Techniques in LC-MS
A Seminar as a part of curricular requirement
for I year M. Pharmacy I semester
Presented by
Mr. G Chiranjeevi
(Reg. No. 20L81S0706)
Pharmaceutical analysis
Under the guidance/Mentorship of
Dr. P. Ramalingam, Ph.D
Director - R&D Cell ,
Professor of pharmaceutical analysis
And medicinal chemistry
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Raghavendra Institute of Pharmaceutical Education and Research - Autonomous
K.R.Palli Cross, Chiyyedu, Anantapuramu, A. P- 515721 2
Introduction to LC-MS
Problems in combining HPLC & MS
Interface in LC-MS
Types of ionization techniques
Choice of ionization technique
References
contents
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It is an analytical chemistry technique.
combination of physical separation capability of LC (or HPLC) and
mass analysis capability of MS.
It is a method that combines the separation power of HPLC with
detection power of mass spectroscopy.
In LC-MS we remove the detector from the column of LC and fit
the column to interface of MS.
In the most of the cases the interface used in LC-MS are ionization
source.
Introduction to LC-MS
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HPLC is a method for separating a complex mixture in to its
components.
High sensitivity of mass spectroscopy provides the information
for identification of compounds or structural elucidation of
compounds.
As the metabolites appear from the end of the column they
enter the mass detector .
Where the solvent is removed and the metabolites are ionized.
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MS work by ionizing molecules and then sorting and
identifying the ions according to their mass-to-charge
ratio(m/z).
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Problems in combining HPLC & MS
HPLC MS
• Liquid phase operation
• 25 – 50 deg Celsius
• No mass range limitations
• Inorganic buffers
• 1 ml/min eluent flow is
equivalent to 500ml/min of gas
• Vacuum operation
• 200 – 300 deg Celsius
• Up to 4000 Da for quadrupole
mass spectroscopy
• Requires volatile buffers
• Accepts 10 ml/min gas flow
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• LC-MS system include a device for introducing samples an
interface for connecting such device.
• If the LC unit is simply connected directly to the MS unit , the
liquid mobile phase would vaporize, resulting in large amount of
gas being introduced into the MS unit.
• This would decrease the vacuum level and prevent the target
ions from reaching the detector. So interfaces to be used.
• In this interface neutral liquid molecules converted to gas phase
ions also.
Interface in LC-MS
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• Mass spectrum is significantly depends up on the ionization
method
• Variations in the spectrum is introduced in the form of
1. number of peaks
2. intensity of peaks
• It can be can be categorized into two parts
Hard ionization technique
- high energy & increased fragmentation
Soft ionization technique
- low energy & decreased fragmentation
Types of ionization techniques
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methods
Gas phase Desorption Evaporation
Thermospray
ESI
APCI
APPI
Electron ionization(EI)
Chemical ionization(CI)
Field desorption
FAB
MALDI
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It is also known as electron bombardment ionization.
Main function of this technique is to convert the gaseous sample
into molecular ions.
It is hard ionization technique because it will produce 70eV.
Ionization potential of organic compounds are approximately
8eV - 15eV.
Due to high energy, large number of fragment ions produced
from molecule ion.
Electron ionization(EI)
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Operating at 10-6 tarr
Electron ionization process
Electron ionization
process
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It is a gaseous phase method and soft ionization technique.
Less fragmentation & give intensive peak of molecular ion(i.e
more no. of M+)
Some molecules like alcohols, ethers, esters, amino acids etc..
Are highly fragmented in EI.
So molecular peaks will not be detected
To get proper molecular peaks we are using CI
Chemical ionization (CI)
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Steps of chemical ionization
1) Introduction of carrier/reagent gas into the ionization source at
slightly high pressure (1 torr)
Ex. Methane, ammonia, isobutane
2) Ionization of carrier gas by electron impact from the ionization
source
CH4 + e-
CH4
.+ + 2e-
CH4
.+
CH3
+ + H+
3) CH4
. + & CH3
+ are primary ions, these will react with excess CH4 & it
will produce different type of secondary ions.(i.e. CH5
+, C2H5
+, C3H5
+)
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CH3
.
CH4
.+ + CH4 CH5
+ +
CH5
+ + CH4 C2H5
+ + H2
C2H5
+ + CH4 C3H5
+ + 2H2
4) Secondary ions will react with analyte molecule & form ions by
three ways.
a)Proton transfer
b)Hydride transfer
c)Electrophillic addition
M + CH5
+ [M-H]+ + CH4
M + C2H5
+ [M-H]+ + C2H4
MH + C2H5
+ [M-1]- + C2H6
M + CH3
+ [M-CH3]+ or [M+15]+
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It is a soft ionization technique under desorption method.
FAB can be used up to 10,000 Da molecular weight.
It is generally used for the determination of mol.wt of peptides.
Fast atom bombardment (FAB)
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Characteristics of the matrix
• It should be non volatile
• Less vapour pressure liquids
Ex. Glycerol, Thioglycerol, 3-Nitrobengyl alcohol, Diethanolamine &
Triethanolamine.
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It is a soft ionization technique under desorption ionization
method.
which uses pulsed LASER (Light Amplification by Stimulated
Emission of Radiation) beam.
It used to determine the mol.wt of peptides, antibodies,
protein, molecules etc. Up to the size of 300Da.
Matrix assisted LASER desorption ionization
(MALDI)
- - - - -
- - - - -
- - - - -
v
vv
v
v
v
- - - - - -
- - - - - -
- - - - - -
- - - - - -
- - - - - -
- - - - - -
- - - - - -
- - - - - -
- - - - - -
v
v
v
v
v
v
v
v
+
Sample soln
0.1 mg/ml
Matrix soln
10 mg/ml
Mixture soln
Co-crystallization
Crystallized
sample-matrix mixture
Ratio
1 : 1000 to 10000
- - - - - -
- - - - - -
- - - - - -
- - - - - - - - - - - - - - - - - -
- - - - - -
- - - - - -
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Matrix materials :-
Nicotinic acid, DHB, cinnamic acid derivatives.
Matrix material should have low mol.wt, acidic nature, strong laser
beam absorption polar functional groups.
0.5 – 20 nano sec. Exposure time
by Protonation
(ToF)
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LASER beams :-
337nm:-Nitrogen laser of UV-range
355nm:- frequency tripled Nd : YAG
(Neodymium : Yherium, Aluminium, Garnet)
326nm:- frequency quadrupled Nd : YAG
294nm:- IR laser produced by Er : YAG
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Then increasing the electric field on the droplet surface
Solvent continues to evaporate
Fine mist of charged droplets are created
A nebulizer gas flows from outside the capillary to spray the sample
Applying a high voltage about 3kv to 5kv
ESI draws sample solution to the tip of capillary tube
Electrospray ionization(ESI)
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21
These gas phase ions enter into the mass analyser
The droplets eventually become small enough that the
sample ions are liberated into the gas phase
As this evaporation and fission cycle is repeated
Fission of molecules occurs
It is softest ionization method , used for highly polar, least
volatile/thermally unstable compounds.
Ex. peptides, proteins, lipids, oligosaccharides.
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It is based on the mechanism of evaporation and carried out at
atmospheric pressure.
Combination of CI & ESI.
Generally linked with chromatographic instrument like HPLC.
Sample injected throw capillary tube, converted into sprayed
droplet & finally analyte.
Solvent vapour due to heating by N2.
We use corona discharge needle or β-particle emitter for
ionization, it will ionize the solvent vapour molecule.
Some times analyte matter also may ionized by the electrode.
Atmospheric pressure chemical
ionization (APCI)
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Due to collision of ion molecule charge transfer b/w solvent and
analyte takes place & analyte ion produced.
APCI may produce both +ve [MH]+ & -ve [M-1]- ions.
It used to analyse polar, thermostable substance with molecular
weight less than 1500Da.
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It is based on the mechanism of evaporation and carried out at
atmospheric pressure.
APPI is similar to APCI, but ionization in APPI is due to photons
generated by UV light krypton lamp.
Sample injected throw capillary tube, converted into sprayed
droplet & finally analyte.
Solvent vapour due to heating by N2.
Desolvation gas (heated N2) will be supplies which will convert
the sprayed droplets into the form of vapours of analyte and
solvent.
Atmospheric pressure photoionization
(APPI)
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Photons emitted by the krypton lamp have specific
energy i.e. 10eV
Which is sufficient to ionize other atmospheric gas
present, due to low energy.
Photons of 10eV will not ionize other atmospheric gas
present, due to low energy.
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Photons will ionize the analyte by three mechanisms
1)Direct APPI:-
2)Indirect APPI:-
3)Dopant assisted APPI :- Toluene is used as dopant to
increase the percentage of molecular ion.
M + hv M . + + e-
S + hv S . + + e-
M + S. + M+ + S.
D + hv D . + + e-
M + D. + M+ + D.
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• ESI
- polar compounds
- Large molecules, proteins,
polymers
- Fragile molecules
• APCI
- Low polarity
- < 2000u
- Small molecules
• APPI/ASAP
- Non polar molecules
- ASAP can analyse low polar
high mass molecule (pyrolysis)
Choice of ionization technique
Atmospheric solid analyse probe (ASAP)
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