1. List of Training Modules
❖ PCR & Real time PCR techniques and its applications
• Module 1 (Culturing methods, Microbial isolation techniques, Media optimisation,
Storing of Microorganisms) [Title: Basic Microbial techniques and their storage]
• Module 2 (Microbial culture techniques, Growth curve analysis, Nucleic acid
extraction, Electrophoresis separation, Polymerase chain reaction (PCR), Primer
designing and standardisation) [Title: Basic Microbial and Molecular techniques]
• Module 3 (Microbial nucleic acid extraction and Quantification, Primer designing and
standardisation, Real Time PCR: Gene Expression studies, Result Interpretation)
[Title: Microbial Gene Expression studies]
• Module 4 (Human nucleic acid extraction and Quantification, PCR, Primer designing
and standardisation, Result interpretation) [Title: Human Gene quantification studies]
• Module 5 (RNA isolation, Electrophoresis, cDNA conversion, Primer designing and
standardisation, Real time PCR, Result interpretation) [Title: Human Gene
Expression studies]
Cost of Each modules and their Combos
Module 1 - Rs. 6,000/- If taking two modules:
Module 2 - Rs. 10,000/- Module 1 and 2 - Rs.15,000/-
Module 3 - Rs. 15,000/- Module 2 and 3 - Rs.22,000/-
Module 4 - Rs. 10,000/- Module 1 and 3 - Rs.15,000/-
Module 5 - Rs. 15,000/- Module 4 and 5 - Rs.22,000/-
If taking all modules:
(Module -1, 2 and 3) – Rs. 28,000/-

2. 1.Molecular Cloning Technique (30,000/- per head)
➢ Media preparation and vector selection
➢ Bacteria culture and Maintenance
➢ Preparation of Competent cells
➢ Restriction digestion
➢ Ligation and Transformation
➢ Small scale isolation of plasmid DNA
➢ Large scale isolation of plasmid DNA
➢ Restriction digestion
➢ Ligation
➢ Confirmation by Gel Electrophoresis
➢ Elusion of DNA from gel
➢ Protein Expression studies
➢ Protein Purification
2.Proteomics (30,000/- per head)
➢ Culture maintenance
➢ Extraction and estimation of protein
➢ Purification of protein
➢ SDS-PAGE

3. ➢ Staining
➢ Western Blot
➢ Western Blot visualization
3.Cell Culture Techniques (30,000/- per head)
➢ Introduction of basic components of Cell culture and environment
➢ Media preparation
➢ Sterile technique and contamination control
➢ Specific cell types and their requirement
➢ Culture of cells in attachment and suspension and scaling up of cultures
➢ Passaging of cells
➢ Study of in-vitro cell growth behaviour
➢ Determination of cell growth
➢ Cell viability and cell proliferation assay techniques
➢ Cryopreservation and retrieval of cells from frozen storage
4. Functional studies related to Cancer (50,000/- per head)
➢ Introduction of basic components of Cell culture and environment
➢ Media preparation
➢ Sterile technique and contamination control
➢ Culture of cells in attachment and suspension and scaling up of cultures

4. ➢ Passaging of cells
➢ Soft agar assay
➢ Clonogenic assay
➢ Wound healing assay
➢ Proliferation assay
➢ Invasion/Migration assay
➢ Angiogenesis
5. DNA damage and repair assays (50,000/- per head)
➢ Introduction of basic components of Cell culture and environment
➢ Media preparation
➢ Sterile technique and contamination control
➢ Culture of cells in attachment and suspension and scaling up of cultures
➢ Passaging of cells
➢ DNA damage marker studies by western blot
➢ DNA fragmentation
➢ Comet assay
➢ Pulse field gel electrophoresis
➢ Halo assay
Special fee offers:
Module 1 & 2 for Rs. 55,000/-
Module 3 & 4 for Rs. 70,000/-

5. Module 3 & 5 for Rs. 70,000/-
Module 1, 2 & 3 for Rs. 80,000/-
Module 3, 4 & 5 for Rs. 1,15,000/-
For Projects- Duration- 10-15 days