This month’s newsflash focuses on our recombinant Fab antibodies. Find out more about their clinical relevance and see new performance data for Cystatin C, Myeloperoxidase and C Reactive Protein recombinant Fab antibodies.

1. Newsflash
April 2014
New Antibody Data Available
Cystatin C Fab Antibody
CRP Fab Antibody
Myeloperoxidase Fab Antibody
Our Catalogue
Monoclonal Antibodies
Polyclonal Antibodies
Recombinant Fab Antibodies
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2.  Be confident in your results when you
choose BBI’s monoclonal antibodies for
your next assay:
 Tested in ELISA
 Tested in lateral flow
 Recommended pairings provided
 Strong customer feedback
 We have now introduced highly sensitive
recombinant Fab antibodies to improve
your next generation assays.
 Custom development and manufacturing
services to meet your individual
requirements also available.
Why choose BBI ?
Quick Links
Recombinant Fab
Technical Information >
Cystatin C Fab mAb >
CRP Fab mAb >
MPO Fab mAb >
Monoclonal Antibodies >
Polyclonal Antibodies >
Recombinant Fab
Antibodies >
How to Order >

3. What are the advantages of recombinant Fab
antibodies?
Standard Monoclonal Antibodies
Recombinant Fab Monoclonal
Antibodies
Initial Screen 100’s of clones screened  1,000,000,000 clones screened
Matched Pairing Best few antibodies tested for pairing
 >4500 antibody pairs tested
(Luminex platform)
Affinity micromolar as standard  picomolar as standard
Sensitivity microgram/ml as standard  picogram/ml as standard
Validation Purified Antigen  Clinical samples
Conjugation
May interfere with antigen binding
region
 Engineered Cysteine residue
ensures no interference
Production
Cell culture - slow, expensive,
unreliable Ascites - slow, uses many
animals, illegal in EU
 E. coli - fast, reliable, easy to scale-
up, simple storage
Antigen binding sites per
mg of purchased product
Standard  36% more
For more technical info visit our website >
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4. Example: Calcitonin recombinant Fab
antibody
 Patients with calcitonin levels >100 pg/mL
have a high risk for medullary thyroid
carcinoma (~90%–100%) Toledo et al.,
(2009).
 Our Calcitonin antibody pair detects at a
range of 6 to 2000 pg/mL.
 These highly sensitive
antibodies are ideal for
evaluating risk of this carcinoma.
“Reduce your time and
money on reagents with
unproven performance – our
results speak for
themselves.”
See the demonstrated clinical relevance
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5. Antibody Spotlight
NEW DATA – Cystatin C recombinant Fab antibody
Antibody Range of Detection*
BR175-G8D9 165 – 9073 ng/ml
BR175-H4C8 165 – 9073 ng/ml
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 Can detect the presence of antigens down to 165 ng/ml.
 Can be used in a competitive ELISA or Lateral Flow, without need for pairing.
 Can be conjugated for use as a detection antibody.
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0.00 0.02 0.04 0.08 0.16 0.32 0.63 1.25 2.50
SignalStrength(RannScore)
Cystatin C Antigen Concentration (µg/ml)
Competitive Lateral Flow Data for
BR175-H4C8
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SignalStrength(RannScore)
Cystatin C Antigen Concentration (µg/ml)
Competitive Lateral Flow Data for
BR175-G8D9
*determined using ELISA, concentrations outside this range have not necessarily been tested .

6. Antibody Spotlight
NEW DATA – CRP recombinant Fab antibody
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0
200
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Peakheight
CRP conc (ng/mL)
Comparison of BR228-D4A3 with commercially
available antibodies
BR228-D4A3
Competitor A
Competitor B
Flu Fab
Reader
CRP conc (ng/ml)
100 75 50 25 10 5 2 1 0
BBI antibody, BR228-D4A3
Competitor B antibody
Competitor A antibody
Visual
Lateral Flow: Comparison of BR228-
D4A3 with commercially available
antibodies
 Dynamic range of antigen detection of 151 - 23100ng/ml determined by ELISA.
 Can be used in a competitive ELISA, without need for pairing.
 BR228-D4A3 antibody, is able to detect down to < 2ng/ml in lateral flow allowing you to
produce high sensitivity assays.

7. Antibody Spotlight
NEW DATA – Myeloperoxidase (MPO) recombinant Fab
antibody
Recommended Sandwich Pairing
Capture Antibody Detection Antibody
BR240-N5G6 BR240-D8B4
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 Can detect the presence of antigens at 2 – 1200ng/ml in ELISA.
 They have now been tested in Lateral Flow.
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SignalStrength(RannScore)
MPO Antigen (ng/ml)
Lateral Flow for BR240-D8B4 & BR240-
N5G6
Order your evaluation samples
today:
info@bbisolutions.com
USA: 1-800-423-8199
Intl: +44 (0) 2920 747 232

8. Our Catalogue | Monoclonal Antibodies
Cardiac Infectious Disease Norovirus
CK-MB C. difficile Toxin A PBP2a
D-Dimer C. difficile Toxin B TB
Myoglobin Chikungunya
Troponin I Dengue NS1 Pregnancy
H. pylori AFP
Diabetes HAV
Hb HBV Tumour
HbA1c Hepatitis B CA125
HIV p24 CA15-3
Drugs of abuse HIV gp36 CA19-9
THCΔ9 HIV gp41 Calcitonin
Influenza A
Hormones Influenza B Immunoglobulins
DHEA-S Leptospira IgA
Cortisol Listeria IgE
Progesterone Malaria HRP2 IgG
PTH Malaria LHD (pan) IgM
Malaria LHD (Pf)
Malaria LDH (Pf & Pv) Return home >

9. Our Catalogue | Polyclonal Antibodies
Inflammation Pregnancy Renal
CRP AFP A2M
B2M
Hormones Inflammatory Cystatin C
HCG A1AT
αHCG
Haematology Metabolism
Immunoglobulins Haptoglobin Ceruloplasmin
IgA Ferritin
IgM Haemoglobin
IgG
Albumin
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10. Our Catalogue | Recombinant Fab Antibodies
* Detection Ranges are assay dependent and optimised for specific
clinical conditions, therefore may be improved with optimisation
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Biomarker Detection Range* Capture Antibody Detection Antibody
HSP60 0.1 - 1200 ng/mL BR100-G2A7 BR100-H4C2
Adiponectin 0.1 - 525 ng/mL BR101-P7E2 BR101-M3H8
Adiponectin 230 - 59118 ng/mL BR101-H2B4 (Competitive ELISA)
Angiopoietin 2 0.001 - 97 ng/mL BR103-P4E8 BR103-V5F2
Big Endothelin 1 0.001 - 1.8 ng/mL BR104-D6A4 BR104-H1A4
Calcitonin 0.006 - 2 ng/mL BR388-G6B1 BR388-D5C4
Cathepsin D 0.03 - 150 ng/mL BR107-F5G2 BR107-K6D3
Eotaxin 0.007 - 2 ng/mL BR108-M7H3 BR108-K2D9
Chondrolectin 0.9 - 21 ng/mL BR111-P8G3 BR111-T6B1
CKBB 0.15 - 33 ng/mL BR112-V6F6 BR112-Z1C4
Proinsulin C-Peptide 0.09 - 38 ng/mL BR143-G5D3 BR143-K7C9
CRP 151 - 231000 ng/mL BR228-D4A3 (Competitive ELISA)
Cystatin C 165 - 9073 ng/mL BR175-G8D9 (Competitive ELISA)
Cystatin C 165 - 9073 ng/mL BR175-H4C8 (Competitive ELISA)
E. histolytica not tested BR116-K9F7 BR116-G3A1
E-selectin 0.05 - 235 ng/mL BR117-K2E7 BR117-H5B6
FABP Intestine 0.65 - 100 ng/mL BR115-F7G7 BR115-G6B9
FABP Liver 0.03 - 90 ng/mL BR106-G5A2 BR106-P9F5

11. Our Catalogue | Recombinant Fab Antibodies
* Detection Ranges are assay dependent and optimised for specific
clinical conditions, therefore may be improved with optimisation
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Biomarker Detection Range* Capture Antibody Detection Antibody
G. lamblia not tested BR120-P3F2 & BR120-T1G8
Human Fetal Hemoglobin 1.9 - 305 ng/mL BR121-T5H9 BR121-V5A7
Leptin 3.9 - 691 ng/mL BR123-M4D6 BR123-P4G2
MMP7 0.1 - 293 ng/mL BR124-S4E3 BR124-T6G8
MMP9 Active 11 - 183 ng/mL BR125-N5F7 BR125-M6D6
MPO 2 - 1200 ng/mL BR240-N5G6 BR240-D8B4
Neutrophil Elastase 0.008 - 33 ng/mL BR127-H2A4 BR127-G7C2
Free PAPP A 0.3 - 299 ng/mL BR129-M5E3 BR129-F2H4
Pentraxin 3 4.6 - 876 ng/mL BR130-H2C3 BR130-K6D6
PGDS 0.2 - 85 ng/mL BR131-M6H7 BR131-P7F7
PSAP A 0.013 - 123 ng/mL BR132-T5G4 BR132-S6E4
PSAP B 0.3 - 1500 ng/mL BR133-V6B5 BR133-V4D1
RBP 4 350 - 500000 ng/mL BR134-Z7D3 (Competitive ELISA)
S100 Beta 0.04 - 5.6 ng/mL BR332-G4B9 BR332-F5H7
ST2 1.39 - 97 ng/mL BR139-S7B7 BR139-V8D4
ST2 0.08 - 31 ng/mL BR139-T3G5 BR139-V8D4
Tie2 0.8 - 600 ng/mL BR140-Z7A5 BR140-D9D7

12. How to Order
Please get in touch to arrange
a quote for any of our products.
info@bbisolutions.com
USA: 1-800-423-8199
Intl: +44 (0) 2920 747 232
Quote on ordering – NF APR
BBI Solutions
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