ophthalmology

1. EYE DONATION

2. INCIDENCE OF BLINDNESS

3. EYE PLEDGING
 Eyes should not be removed from a living person
 Eyes should not be purchased or sold
 Individuals can register in the nearest eye bank for
donating their eyes after death.
 Eyes are collected after getting death information
and consent from nearest relatives.

4. PLEDGE FORM
 Name :
 Gender :
 Date of Birth : Submit (mm/dd/yyyy)
 e-mail id :
 Address :
 Country :
 State :
 City :
 Pincode :
 Phone No (Residence) :
 Mobile :
The information furnished above is correct to my knowledge
I PLEDGE

5. WHO CAN DONATE
 Any age
 Any sex
 Any religion
 Even if not pledged while alive
 Spectacle wearers
 Undergone cataract or other eye surgeries
 Diabetics & hypertensives

6. WHO CANNOT
 HIV +
 HEPATITIS B , C
 Rabies
 Syphilis
 Creutzfeldt Jacob Disease
 Alzeimer’s Disease
 Septicemia
 Leukemia
 Death From Unknown Cause

7.  Corneal diseases
 Intraocular tumours eg. Retinoblastoma,melanoma
 Iridocyclitis
 Endophthalmitis

8. DONOR REMOVAL

9. TIME OF REMOVAL
:
 Eyes must be collected within 6 hours of death.

10. BEFORE REMOVAL
 dgg

11. INFORMATION NOTED
 AGE OF DONOR
 TIME OF DEATH
 CAUSE OF DEATH

12. Methods of REMOVAL
 ENUCLEATION

13.  CORNEO-SCLERAL BUTTON EXCISION

14.  5 ml blood withdrawn from donor to screen for
infectious diseases like HIV, Hepatitis

15. TRANSPORT TO EYE BANK
MOIST CHAMBER METHOD
 Glass bottles with metal stand

16. EVALUATION OF DONOR CORNEA
 SLIT LAMP BIOMICROSCOPY
 SPECULAR MICROSCOPY- endothelial cell count

17. GRADING OF CORNEA

18. STORAGE
 SHORT TERM (2-4 days)
 INTERMEDIATE TERM ( 2 weeks)
 LONG-TERM (months-years)

19. SHORT TERM
 MOIST – CHAMBER METHOD (2 Days)
 McCAREY KAUFMAN MEDIUM (4days)

20. MOIST – CHAMBER METHOD
 The whole donor eye is kept in a sterile jar filled with
saturated moist atmosphere at 4°C
 Upto 2 days
 Advantage – simplest, cheap, esp in developing
countries.
 Disadvantage – intact eye, hence endothelium
exposed to aqueous changes.

21. McCAREY KAUFMAN MEDIUM
 Described by McCarey and Kaufman
Contents
 Tissue culture TC-199
 5% dextran-40
 HEPES buffer
 Gentamycin
 Pink in colour
 Upto 4 days.
 Only Corneoscleral button

22. INTERMEDIATE TERM
 Corneal storage media containing chondroitin
sulfate
 Enhanced corneal dehydration.
 OPTISOL
 K-SOL
 DEXSOL
 Upto 2 weeks.

23. LONG TERM
 ORGAN – CULTURE METHOD
Corneas are incubated in tissue culture medium
supplemented with fetal calf serum, antibiotics, and
antimycotics at 30–37°C.
 Upto 30 days
 CRYOPRESERVATION:
Freezing the donor cornea stored in liquid nitrogen.
Upto 1 year

24. UTILIZATION OF DONOR CORNEA
 KERATOPLASTY
Penetrating – full thickness graft
Lamellar – partial thickness graft
 Optical – to restore vision
 Therapeutic – remove infected tissues in non-healing
ulcers
 Tectonic – restore integrity of eyeball
 Cosmetic