This document describes immunohistochemistry staining of mouse brain tissue to label various proteins. It includes details on primary and secondary antibodies used to stain for glial fibrillary acidic protein (GFAP) in astrocytes of the hippocampus and aquaporin 1 and 4 (AQP1, AQP4) in the choroid plexus and ventricles. References are provided on the role of aquaporins and the choroid plexus in cerebrospinal fluid production and homeostasis. Future work proposed includes RNA sequencing of choroid plexus from LXRβ knockout and wild type mice.