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CULTIVATION AND CONSERVATION OF
MEDICINAL PLANTS THROUGH
CUMMINITY PARTISIPATION
CH. MUHAMMAD MUSLIM
MEDICINAL PLANTS BOTANIST
PAKISTAN FOREST INSTITUTE
PESHAWAR
OBJECTIVES
 Qualitative & quantitative survey of medicinal
plants & regeneration problems in the forest.
 Cultivation trials of medicinal & aromatic plants
for finding out agronomical data for
improvements of crops.
 To assess the demand & supply of herbal drug
plants in the market.
 To disseminate knowledge about the availability
of medicinal plants to interested public &
pharmaceutical industries.
Someone once said there are
only two types of fools: one
professes “this is old and
therefore good” and the other
says “This is new and
therefore better”
 Point: Use your scientific evaluations in
the matter, it is a matter of life or death!
Use of Medicinal Plants
 Use of medicinal plants developed from
informal experimentation and based on a
general familiarity with medicinal plants.
This knowledge was amassed via
experimentation over many generations
and was handed down orally from person
to person – often woman to woman in
traditional cultures.
Surveys
Qualitative
Surveys
Quantitative
Surveys
Qualitative surveys 1950-1964
The medicinal plant branch carried out
pioneer work on surveys of medicinal plants
to determine medicinal plants potential in
their natural habitats in different parts of
country since its inception
Survey of
Baluchistan:1950-52
Survey of Kuram
Agency:1952-54
Survey of Kalat:1954-
55
Survey of Northern areas, Azad
Jammu &Kashmir:1955-57
Survey of Tharparker:1957-58
Survey of Murree & Hazara Hill
Forests:1958-62
Survey of Swat State:1963-64
MEDICINAL PLANTS OF FOUR
PROVINCES
• Ephedra nebrodensis (Asmania),
Juniperus excelsa (Abhel),
Withania coagulans (Panir dodi),
Thymus serphyllum (Ban ajwain),
Prunus amygadalus (Badam
talkh), Berberis lycium (Darhald),
Glycyrrhiza glabra (Mulethi),
Bunium persicum (Kala zeera),
Perovskia abrotanoides (San
shob) and Zuffa officinalis (Zufa)
Baluchistan:
Sindh
Cassia angustifolia (Sanna),
Ricinus communis (Castor),
Commiphora mukul (Gugal),
Citrulus colocynthis (Hantal),
Cassia fistula (Amaltas) and
Rhazya stricta (Vena)
Punjab
Fumaria indica (Shatra), Cassia
absus (Chaksu), Adhatoda vasica
(Berg bansa), Datura alba (Datura),
Plantago ovata (Isabghol), Ricinus
communis (Castor), Abutilon indicum
(Pili buti), Solanum nigrum (Mako)
and Peganum hermala (Hermal)
Khyber Pakhtunkhwa
Artemisia maritima (Afsanteine),
Hyoscyamus niger (Ajwain khurasani),
Dioscorea deltoidea (Kanis), Valeriana
jatamansi (Mushk-bala), Berberis lycium
(Darhald), Atropa acuminata (Angoor-i-
shifa), Podophyllum hexandrum
(Bankakri), Aconitum heterophyllum
(Atees talkh), Bergenia ciliata (Zakham-i-
hayat) and Viola sepens (Banafsha)
NORTHERN AREAS AND AZAD
KASHMIR
Dioscorea deltoidea (Kanis), Saussurea costus
(Kuth), Atropa acuminata (Angoor-i-shifa),
Valeriana jatamansi (Mushk-bala), Aconitum
heterophyllum (Atees talkh), Datura stramonium
(Datura), Colchicum luteum (Suranjan-e-talkh),
Ferula sp. (Hing), Rheum emodi (Revand chini),
Podophyllum emodi(Bankakri), Viola serpens
(Banafsha), Gentiana kurroo (Nilkant), Lavatera
kashmiriana (Resha khatmi) and Swertia chirata
(Chirata)
Plants being used by various system of medicines
Distribution of medicinal plants by habits
Distribution of medicinal plants by families
Break up of medicinal plant by their parts utilized
MANAGEMENT OF MEDICINAL AND AROMATIC PLANTS
S # Group – A Group – B
Long duration plants of higher
elevations (2000– 3000m)
Short duration plants of lower
elevation and plains (Up to 1000m)
1. Dioscorea deltoidea
(Kanis)
Plantago ovata
(Ispaghol)
2. Podophyllum emodi
(Bankakri)
Carum copticum
(Ajwain)
3. Aconitum heterophyllum
(Atis)
Nigella sativa
(Kalonji)
4. Valeriana wallichii
(Mushkbala)
Foeniculum vulgare
(Sonf)
5. Berberis lycium
(Darhald)
Psoralea corylifolia
(Babchi)
Cultivation of Medicinal Plants
Atropa acuminata
 Local name : Angoor-i-shifa
 Family : Solanaceae
 Status : Herb
 Medicinal uses:
The leaves and roots are used as sedative,
antispasmodic and narcotic. The dried leaves
are smoked as an antispasmodic. It is usually an
important constituent of preparations
rheumatism and inflammations as of the breast.
Atropa acuminata
Atropa acuminata
Atropa acuminata
Seed germination studies on Atropa acuminata at PFI, Peshawar
Soil media Percentage germination
Soil, sand & manure (33% each) 44
Soil & manure (50% each) 36
Sand & manure (50% each) 38
Source: Sharan (elevation 2800m)
Exp. Design RCB Design
Replications 3
5 gm sample 8 berries
Average number of seed (5gm) 2081
Size of Petri dish 15cm diameter
Seed sown in each Petri dish 100
Temperature 13-300C
Date of sowing October 2003.
Seed germination started after 40 days & took 27 days for
complete germination
Germination of treated & untreated seeds under field conditions
 To increase the %age of germination & to reduce log time taken for
germination, seeds were treated with 200ppm gibberellic acid for 30
minutes.
 Seed started germination after 20 days as compared to untreated seeds
which took 42 days.
Treatment
Date of
sowing
No. of days
taken for
initiation of
germination
No. of days
taken for
complete
germination
Germination
%
Untreated seed 5.10.2003 42 143 53
Seed soaked in
200ppm GA for
30 minutes
27.11.2003 22 98 93
Experiments to test the effect of application of NPK (i) N 150+P60+K30 kg/ha
(ii) N200+P90+K60 kg/ha & to determine the effect of various spacings
(45x30, 45x45, 60x45, 60x60cm) on the growth, leaves & root yield of A.
acuminata at kuzagali.
Fertilizer having NPK in two
combinations
F1 N150+P60+K30 kg/ha
F2 N200+P90+K60 kg/ha
Spacings (cm) 45x30, 45x45, 60x45, 60x60
Exp. Design Split plot design
Replications 4
Plot size 12m2
Total seedlings 1496
Date of sowing April, 2003
Mean fresh leaves yield of A. acuminata (kg/plot 12m2) as affected by
cultural & fertilizer trials
Fertilizer treatment Spacing (cm) Fertilizer mean
45x30 45x45 60x45 60x60
F1 0.67 0.57 0.58 0.34 0.54
F2 0.79 0.65 0.60 0.46 0.62
Spacing mean 0.75 0.61 0.56 0.40
Results indicated that plants spaced at 45x30 &45x45cm resulted in a
significant increase in yeild of leaves (2.79 & 2.64 kg/plot) or 2.33 & 2.20
tonnes/ha per annum respectively as compared to wider spacings 60x45 &
60x60cm (2.10 & 1.94 kg/plot) or 1.75 & 1.62 tonnes/ha per annum.
Application of split doses of NPK (N200, P90 & K60 kg/ha) gave higher
yield of fresh leaves (2.49kg/plot) or 2.08 tonnes/ha per annum as
compared to lower doses of NPK fertilizer, i.e., N150, P60 &K30 kg/ha(2.24
kg/plot) or 1.87 tonnes/ha per annum.
Mean fresh root yield (kg/plot) as affected by various
spacing & fertilizer treatments
Fertilizer treatment Spacing (cm) Fertilizer mean
45x30 45x45 60x45 60x60
F1 3.38 2.82 2.41 2.26 2.72
F2 3.75 3.15 2.81 2.46 3.04
Spacing mean 3.57 2.98 2.61 2.36
Yield results indicated that higher root yield was obtained in plants
spaced at 45x30cm as compare to other spacing. Application of split
doses of NPK (N200, P90, K60) gave higher yield of roots as compared
to lower doses of NPK (N150, P60, K30).
Recommendations
 Fresh leaves yield was low during first year but it
increased with the duration of time from second to third
year growth. Root took 3 years growth period to attain
commercial size for harvesting & utilization.
 The regeneration percentage of A. acuminata plant
ranged from 40-66% as observed in various
experiments.
Colchicum luteum
 Local name : Suranjan-talkh
 Family : Colchicaceae
 Status : Herb
 Medicinal uses:
The corms are used as carminative, laxative
and aphrodisiac. It is also given in gout,
diseases of liver and spleen. The corms are
also employed as an external application for
lessening inflammation and pain.
Colchicum luteum
Colchicum luteum
Effect of planting different portions of corms (Daughter, Mother &
Full corms) of Colchicum luteum on the regeneration of corms at
Peshawar.
Treatments
 Source: Abbottabad
 Portion of corm: Daughter, Mother & Full corm
 Mode of planting: Ridges
 Plot size: 32m2
 Plant to Plant distance: 15cm
 Row to Row distance: 30cm
 Replications: 5
 Number of rows: 10
 Layout: RCB Design
 Date of sowing: 26thFebruary, 2004.
Mean sprouting percentage in T1 (Daughter) 103.2, T2 (Full) 73.4 and T3
(Mother) 60.0 was recorded respectively. Highly significant differences
were observed in mean sprouting of treatments T1 (Daughter), T2 (Full)
and T3 (Mother) respectively. Therefore T1 (Daughter) corms are more
suitable for cultivation of corms of C. luteum.
Regeneration of Colchicum luteum (sprouts out of 250 corms) recorded on
February 28, 2004.
Replications T1 (Daughter) T2 (Full) T3 (Mother) Total
R1 109 80 55 244
R2 120 63 59 242
R3 105 75 65 245
R4 89 70 59 218
R5 93 79 62 234
Total 516 367 300 1183
Mean 103.2 73.4 60.0
Sprouting % 41.28 29.36 24.0
Dioscorea deltoidea
 Local name : Kanis, kildri
 Family : Dioscoreaceae
 Status : Herb
 Medicinal uses:
 The tubers are employed in the treatment of
bilious colic and as a diuretic and expectorant.
They are also used to kill lice and fish poison.
The tubers are a very good source of diosgenin
which is a starting material for hormone
preparation.
Dioscorea deltoidea
Dioscorea deltoidea
Dioscorea deltoidea
Comparative studies on the performance of Dioscorea deltoidea
rhizomes collected from different ecological zones i.e., Kaghan, Murree
& Galliat areas for their growth & yield of rhizomes.
Source: Murree, Galliat & Kaghan
Plat size: 30m2
Experimental Design: RCB
Replications: 6
Plant to plant distance: 30 cm
Row to row distance: 45 cm
Rhizome weight: 25gm
Date of sowing: 2000-06
Comparative studies on the performance of Dioscorea deltoidea
rhizomes collected from different ecological zones i.e., Kaghan,
Murree & Galliat areas for their growth & yield of rhizomes.
Source 3 Years growth 6 Years growth
Galliat 6.60 8.54
Murree 6.27 8.52
Kaghan 5.45 7.62
Comparative mean yield of D. deltoidea (kg/plot)
Yield results indicate that Galliat source gave higher rhizome yield (8.54
kg/plot) followed by Murree (8.52 kg/plot) & Kaghan (7.62 kg/plot) after 6
years growth. These results showed yield result of 3 years and yield of
rhizomes increased with duration of time.
Recommendations
 Galliat source gave better regeneration
percentage and higher yield of rhizomes as
compared to other sources and is suitable for
planting for regeneration purposes.
Podophyllum emodi
 Local name : Bankakri
 Family : Berberidaceae
 Status : Small erect herb
 Medicinal uses:
 The seed are demulcent, stimulant and
nervine tonic. The oil is slightly laxative.
The cake left after extraction of the fixed
oil is used in cases of diabetes. Burnt shell
of almond is used as a tooth-powder.
Podophyllum emodi
Podophyllum emodi
Podophyllum emodi
Podophyllum emodi
Studies to judge the effect of planting different aged
rhizomes (1 year, 2 years, 3 years & full) on sprouting,
growth & yield of P. emodi rhizomes harvested after 4
years growth at Thandiani.
Source: Thandiani
Treatments 1 year, 2 years, 3 years & full
Plot size 6m2
Exp. Design Split plot Design
Replications 4
Total rhizomes planted 39
Spacing 30x60cm
Date of sowing October 2002.
Ages of rhizomes planted
(in years)
Replications
Mean
1 2 3 4
1 0.49 0.46 0.41 0.45 0.45
2 0.56 0.54 0.50 0.46 0.51
3 0.73 0.65 1.52 0.64 0.63
Full 0.77 0.74 0.61 0.68 0.69
Mean fresh yield of rhizomes of P. emodi as affected by planting
various aged rhizomes harvested after 4 years growth (kg/plot)
Recommendations
 Planting of 2 bud portion (2 years old stock) gave large
number of propagule then planting of full, 1 & 3 years
rhizomes.
 Two buds portion would be economical to provide more
number of plants and higher yield can be obtained after
8-10 years growth.
 Podophyllum rhizomes are slow growing and takes 8-10
years for attaining commercial size for harvesting,
therefore, a rotation period of 10 years is recommended
for exploitation of rhizomes.
Saussurea lappa
 Local name : Kuth
 Family : Compositae
 Status : Herb
 Medicinal uses:
Dried roots are aromatic, tonic, stomachic,
carminative, stimulant and useful in asthma,
cough, fever, chest problems and cholera. It is
also used skin diseases and rheumatism. It has
also antiseptic and disinfectant properties. The
root has been much priced as an aromatic
substance.
Saussurea lappa
Saussurea lappa
Saussurea lappa
Germination studies on Saussurea lappa at PFI, Peshawar.
Source: Azad Kashmir
Exp. Design RCB Design
Replications 4
Ten random samples 5gm each 5gm (number of seeds 336)
Two samples treated 200ppm, 100ppm GA (30 minutes)
Third sample Chilling at 0oC (48 hours)
Fourth sample Control
Total tubes sown 1600
Date of sowing January 2000.
Seed germination of S. lappa in various treatments under field conditions
Treatment No. of days taken
for initiation of
germination
No. of days taken
for complete
germination
Seed
germination %
Control 35 65 60
Chilling 21 50 89
200ppm GA 14 40 97
100ppm GA 14 40 91
Maximum germination (97%) was obtained with 200ppm GA followed by
100ppm (91%) & chilling treatment (89%) against control (60).GA
treatment also reduced germination initiation time to 14 days as
compared to control which took 35 days. Similarly chilling also reduced
initiation time to 21 days.
Introduction & adaptability studies on S. lappa to judge the effect of
various spacings on the growth & root yield harvested after 3 & 5 years
growth at Thandiani.
Source: Shardah Forest Division, AJK
Exp. Design RCB Design
Replications 4
Plot size 15m2 (16 plots)
Total seedlings planted 1200
Date of sowing April, 2000
Treatment
Spacing (cm)
2003
3 years growth
2005
5 years growth
45x30 0.84 1.49
45x45 0.58 1.01
60x30 0.76 1.27
60x60 0.47 0.78
Mean 0.66 1.14
Mean fresh yield of S. lappa (kg/plot) as affected by various spacing treatments
Recommendations
 S. lappa plants being perennial requires at least
5 years growth period for development of good
size roots for commercial exploitation, therefore
a rotation of five years may be fixed for its
harvesting and better regeneration in its natural
habitat.
 A spacing of 60x30cm is suitable for artificial
planting in areas where the plants are scarce.
2. Study of medicinal plants among weeds of
wheat
and maize in Peshawar region.
Objectives:
 To identify the weeds of medicinal importance.
 To determine the main constituents & beneficial importance of
weeds.
Study Period: 2010-12
Locations of study:
 Palosi (Peshawar)
 Mardan
 Swabi
 The study is under progress.
1. Survey of drug markets of Peshawar.
Silybum marianum
Family: Asteraceae
English name: Milk thistle
Locality: Wheat field of Malakandher
Part used: Whole herb root,
leaves, seeds.
Medicinal value: Seeds are used in jaundice.
Silybum marianum
Melilotus indica
Family: Fabaceae
Common names:Senji (Urdu), Levanai (Pushto),
India clover (English)
Locality: Wheat Field Malakandher
Part used: Whole plant, Seeds
Medicinal value: Externally applied as poultice or
plaster for swellings. Seeds are
used in bowel complaints and
diarrhea.
Local uses: Used against skin diseases.
Melilotus indica
Euphorbia helioscopia
Family: Euphorbiaceae
Common names:Mandaroo (Pushto), Zahar booti
(Punjabi), Cat's milk
(English.)
Locality: Wheat field Malakandher
Part used: Shoot, leaf, root.
Medicinal value: Seeds with pepper given in
cholera. Milky juice applied to
eruptions.
Local uses: Used in skin diseases.
Euphorbia helioscopia
Fumaria indica
Family: Fumariaceae
Names: Papra (Pushto), Shahtra (Urdu),
Fumitory (English)
Locality: Wheat field of Malakander.
Part used: Whole plant.
Medicinal value:
Plant is used as aperients,
diaphoretic and diuretic.
Fumaria indica
Production of Medicinal Herbs in Collaboration
with Private Sector (PMHPS) Project.
Duration: Five years (60 months)
Commencement date: 1st July 2006
Termination date: 30th June 2011
Sponsoring agency: MinFAL, Islamabad
Staff: Research Assistant (1)
Forester (1)
Driver (1)
Objectives:
 To develop research based package of agro-technology to comply
WHO guidelines on Good Agriculture, Collection and Processing
Practices (GACP).
 Multiplication of registered seed and propagation materials on farmer’s
field.
 Promotion of commercially important medicinal herbs in earthquake hit
areas.
 Survey of crude herbal drug markets of Pakistan to determine supply
and demand position of crude drugs of vegetable origin.
Cultivation of important economic and medicinal plants
Name of crops
Sowing
time
Germination
time
R to R
spacing
(cm)
Estimated
flowering
time
Harvesting
time
Estimated
Yield/ ha.
(kg)
Income
Rs.000
Nigella sativa
(Kalongi)
October 15 days 30 March April-May 550-650 60-70
Linum
Usitatissimum (Alsi)
October
November
8 days 30 February -do- 700-800 30-40
Carum copticum
(Ajwain)
October 12 days 36 March May 500-550 30-35
Foeniculum
vulgare (Sonf)
October 8-10 days 30-45 March April-May 800-900 50-60
Lallementia
royleana
(Tukhm-i-balanga)
September
October
10-12 days 30 February April 450-600 70-80
Plantago ovata
(Isabghol)
September
October
6 days 15-20 February
March-
April
450-500 40-45
Studies to test the effect of various row-to-row spacing
on the seed yield of Carum copticum at PFI, Peshawar.
Objectives: Standardization of suitable row-to-row
spacing for getting higher seed yield.
Material and methods:
Seed source: Peshawar
 Plot size: 20 m2
 Row to row distance: Broadcasting, 24, 36 and 48 cm
 Replications: 4
 Layout: RCB Design
 Date of sowing: 14th October, 2008.
Row to row spacing (cm)
BC 24 36 48
0.561 0.794 1.246 0.866
0.403 0.545 0.610 0.489
0.231 0.325 0.375 0.374
0.245 0.218 0.318 0.324
Mean 0.360 0.470 0.637 0.513
Results achieved indicated that row-to-row spacing of 36 cm resulted in
a significant increase in the seed yield (318.5 kg/ac) as compared to 48,
24 cm and broadcasting (256, 235, 180 kg/ac) respectively. No
significant difference was observed in the mean seed yield of 48 and
24cm row to row spacing and both spacing proved superior over
broadcasting.
Bio-Trace spray on Carum copticum at PFI, Farm
Fertilizer trials on Nigella sativa crop at PFI, Peshawar.
Objectives:
 Determination of appropriate dose of nitrophos fertilizer in order to
get optimum seed yield.
Material and methods:
Seed source: Peshawar.
Plot size: 20 m2
Row-to-Row distance: 30cm
Replications: 4
Layout: RCB Design
Date of sowing: 4th October 2008.
Number of rows: 12
Doses of Nitrophos fertilizer (kg/ac): 0, 50, 75 and 100.
Doses of nitrophos fertilizer (kg/plot)
0
50 75 100
1.736 2.534 3.123 3.012
0.643 1.990 2.128 2.420
Mean 1.189 2.262 2.625 2.716
The application of split doses of nitrophos fertilizer @ 100 kg/ac
gave higher seed yield (1099 kg/ac) as compared to 75 kg
nitrophos fertilizer/ac (1062 kg/ac) and control (481.37 kg/ac).
There was no significant difference in the mean seed yield of 75
and 100 kg nitrophos/ac as both doses were mutually alike.
Nigella sativa
Cultural and fertilizer trials on Linum usitatissimum at PFI,
Peshawar
Objectives:
 To find out water requirement (6 v 3 irrigation) and application of
different doses of nitrophos fertilizer on the seed yield.
Materials and methods:
Seed source: PFI, Farm
Plot size: 30 m2
Row to row distance: 30 cm
Replications: 4
Layout: split plot design
Date of sowing: 9th October 2008
Number of rows: 10
Irrigation interval: fortnightly vs. 3 weeks
Doses of nitrophos
Fertilizer (Kg/ac): 0, 50, 100 and 150 kg
Doses of nitrophos fertilizer (kg/plot)
Irrigation
number
0 50 100 150
6 0.836 1.300 1.600 1.340
3 0.673 0.793 0.873 0.825
Pooled mean for
fertilizer
0.754 1.046 1.236 1.082
Linum crop showed good response to the higher number of
irrigations 6 (fortnightly) as compared to 3 irrigation (3 weeks).
The application of split doses of nitrophos fertilizer @100 kg/ac
gave higher seed yield (500.40 kg/ac) as compared to 50 kg
nitrophos fertilizer/ ac (423.48 kg/ac) and control (305.26 kg/ac).
There was no significant difference in the mean seed yield of
100 and 150 kg nitrophos/ac as both doses were mutually alike.
Experimental plot of Linum usitatissimum at
PFI, Farm
Fertilizer trials on Foeniculum vulgare crop at (Bachai) Swabi
Objectives:
 Determination of appropriate dose of nitrophos fertilizer in order to
get optimum seed yield.
Material and methods:
Seed source: Bara.
Plot size: 20m2
Row-to-Row distance: 45cm
Replications: 4
Layout: RCBD
Date of sowing: 18th October 2008.
Number of rows: 10
Doses of Nitrophos
fertilizer (kg/ac): 0, 50, 75 and 100.
Cultivation of sonf at Bachai (Swabi)
Foenicullum vulgare
Foenicullum vulgare (sonf) plot at Bachai (Swabi)
Cultivation and yield trials of Matricaria chamomilla (gul-i-
baboona) to standardize its cultivation technology
Objectives:
To standardize its cultural requirement and economic feasibility
under Peshawar climatic conditions
Material and methods:
Seed source: Swat
Plot size: 20m2
Replications: 4
Layout: RCBD
Plant to plant distance: 4cm
Date of sowing: 20th October 2008.
Number of rows: 10
Treatment: (20, 30, 40 cm and conventional broadcasting)
Effect of various row to row spacing on the flower yield in kg/plot (20 m2).
Row spacing (cm)
Replication Broadcasting 20 30 40
1 2.000 4.000 5.800 1.800
2 2.010 5.200 5.000 2.000
3 1.990 2.900 4.120 5.010
4 2.220 4.400 6.000 4.000
Mean 2.055 4.125 5.230 3.202
A row to row spacing of 30 cm gave higher yield of fresh flower
heads (5.23) as compared to broadcasting (2.05), 40 spacing cm
(3.20) and 20 cm spacing (4.12) kg/plot respectively.
Matricaria chamomilla
Matricaria chamomilla
Introduction of Glycyrrhiza glabra plantation at
Medicinal Plants Farm, PFI, Peshawar.
Common name:
Mulathi
Glycyrrhiza glabra (Mulaithi) roots were collected from
Mastooj and garam chashma (Chitral) during 2005-06. Six
hundred root cuttings were cultivated at Medicinal Plants
Farm, Peshawar in September 2005-06.
Part used:
Roots.
Area:
1 Kanal
Uses:
Expectorant, cough, jaundice, kidney stones, water-extract
contains glycyrrhizic acid, glycyrrhizin, useful in anti-allergic
diseases. 50 times sweeter than cane sugar.
Glycyrrhiza glabra at PFI, Peshawar
Glycyrrhiza glabra (Mulathi)
Glycyrrhiza glabra (Roots)
Studies to compare the yield of two strains of turmeric
(Curcuma domestica) at Mardan.
Common name:
Haldi
Area:
4 Kanal
Part used:
Rhizome.
Uses:
Anti-Cancer, Anti-HIV, healing property, Hypo-
cholestrolmic, Diabetes, Carminative, aromatic,
tonic, blood purifier.
Studies to compare the yield of two strains of turmeric
(Curcuma domestica) at Mardan
Strains of turmeric:
 Bannu
 Changa-Manga
Plat size: 34m2
Experimental Design: LSD
Replications: Four
Plant to plant distance: 25 cm
Row to row distance: 30 cm
Rhizome size: 4-5 cm
Date of sowing: March, 2009.
Studies to compare the yield of two strains of turmeric
(Curcuma domestica) at Mardan
Strains Yield (Kg/ha)
Bannu 4150
Changa-Manga 4370
 5 kg rhizomes of turmeric were given for
chemical analysis to Dr. Javed Abbas Bangash
of PCSIR laboratories, Peshawar.
Performance of Curcuma domestica (Haldi) at
Mardan
Curcuma domestica
Curcuma domestica
Performance of Curcuma domestica (Haldi) at
Mardan
Zingiber officinale (Adrak)
Zingiber officinale (Adrak)
Adrak at Peshawar
Comparative study of two sources of Rosa
damascena for flower production at PFI,
Peshawar.
Rosa damascena (Desi gulab)
Source:
 Kalar Kahar
 Peshawar
Plot size: 48m2
Row to row distance: 1m
Plant to plant distance: 30cm
Date of sowing: 25-01-2010
Number of cuttings/plot: 70
Total number of cuttings: 560
Experimental design: LSD
Rosa damascena
Studies to test the effect of different doses of nitrophos fertilizer
on the growth and seed yield of Plantago ovata at D.I.Khan
Objectives:
 Determination of appropriate dose of nitrophos fertilizer in order to
get optimum seed yield.
Material and methods:
Seed source: D.I.Khan
Plot size: 20m2
Row-to-Row distance: 45cm
Replications: 5
Layout: RCBD
Date of sowing: 18th October 2008.
Number of rows: 10
Doses of Nitrophos
fertilizer (kg/ac): 0, 50, 75 and 100.
Effect of different doses of nitrophos fertilizer on seed yield of Plantago ovata
Doses of nitrophos (kg/ac) Mean yield in kg/ac
0 176
50 195
75 259
100 268
Application of split doses of nitrophos at the rate of 100 and 75
kg/ha resulted in a significant increase in the seed yield (268 and
259 kg/ac) respectively as compared to 50 kg nitrophos/ac (195
kg/ac) and control treatments (176 kg/ac).
Plantago ovata (Isabghol)
PLANTAGO MAJOR (JANGLI-ISABGHOL)
Survey of Plantago ovata (isabghol) in District Bhawalpur
 Survey of Plantago ovata (Isabghol) cultivation,
harvesting, processing and trading was carried
out in Haroonabad, Hasilpur, Cheshtian,
Dharanwala and Multan under the direction of
Dr. Muhammad Aslam Gill, Project Director,
PMHPS Project.
Land holding capacity of farmers who are growing Plantago ovata
S # Total area (Acres) Number of farmers in this group
1 25-50 10%
2 11-20 15%
3 5-10 75%
From the data in the above table, it is evident that land holding
size (5-10) is the largest (75%).
Tenure status of sampled farms
Stratum Owner (%) Tenant (%) Tenant-cum-owner
All areas 93.9 5.8 0.3
93.9% of the sampled farms are owner operated, 5.8% tenant operated
and the remaining 0.3% are the tenant-cum-owner operated.
Isabghol processing unit
Isabghol processing unit
Isabghol processing unit
Isabghol processing unit
(Interview) isabghol grower
Isabghol processing unit
Constraints associated with the processing of medicinal plants
 Poor agricultural practices
 Poor harvesting and post-harvest treatment practices
 Lack of research on development of high-yielding varieties,
domestication etc.
 Poor propagation methods
 Inefficient processing techniques leading to low yields and poor quality
products
 Poor quality control procedures
 High energy losses due to processing
 Lack of current good manufacturing practices
 Difficulties in marketing
 Lack of local market for primary processed products
 Lack of trained personnel and equipments
 Lack of facilities to fabricate equipment locally
 Lack of access to latest technologies and market information
Training at Haripur
MENTHA LONGIFOLIA (JANGLI PUDINA)
PAEONIA EMODI (MAMEKH)
ASPARAGUS ADSCENDENS (MUSLI-SUFAID)
Linum & Nigella sativa at D.I.Khan
SAUSSUREA COSTUS (KUTH-THALKH)
RHEUM EMOLDI (REVAND-CHINI)
PAEONIA EMODI (Mamekh) ATROPA ACUMINATA (Angoor-i-shifa)
ii. Vulnerable species
Berberis lycium (Kashmal)
Corydalis govaiana (Mamiri)
Bergenia ciliata (Zakham-i-Hayat)
Jurnea macrocephala (Gugal
dhoop)
Heracleum candicans (Kamur)
iii. Extinct species
Elaeganus hortensis (Singli)
Saussurea costus (Kuth)
S. sacra (Jogibadshah)
Onasma bracteatum (Gaozaban)
Corydalis govaiana (Mamiri)
iv. Rare species
Achilles mille folium (Monandu)
Aconitum heterophyllum (Atees)
A. chasmenthum (Patrees)
Lavetera kashmiriana (Resha khatmi)
Rheum emodi (Revand chini)
Viola serpens (Banafsha)
Swertia chirata (Chirata)
Angelica glauca (Chora)
Zanthoxylum armatum (Timar)
Achilles millefolium (Monandu)
Digitalis lanata
(Berg-al-azra)
Saussurea lappa
(Kuth)
Colchicum luteum
(Suranjan-thalkh)
Bauhinia variegata
(Kachnar)
Polygonum amplexicaule
(Anjabar)
Dioscorea deltoidea
(Kanis)
Podophyllum emodi
(Bankakri)
Valeriana wallichii
(Mushkbala)
Acorus calamus (Sakha-waja)
Digitalis lanata and D. purpurea
Lavatera kashmiriana
Valeriana wallichii
Trees of medicinal value
Emblica officinalis
(Amla)
Sapindus mukorrossi
(Ritha)
Aesculus indica Neem
Aesculus indica Neem
Kachnar
WHAT MEASURES CAN BE DONE FOR THE
CONSERVATION OF MEDICINAL PLANTS????
• Law Enforcement to Protect The Forest
 • Reforestation
 • Rangeland Management
 • Land Reforms
 • Awareness Raising
 • Capacity building of local people
 – Collection, Cultivation, Processing and
 Marketing of Medicinal Plants
 • Linkage of collectors with Market
 – Industries (Pharmaceutical and Herbal)
 Dealers
Seed Collection
 Seeds of Atropa accuminata, Saussurea
lappa, Podophylum emodi, Asparagus
adscendens, Saxifrage ciliate and
Fagopyrum esculentum were collected
from Swat, Gilgit Baltistan, Neelam Valley
and Jehlum Valley
To Judge The Effect Of GA On
The Seed Germination Of
Saussurea lappa,
 Date of Sowing;
 Treatment ; Control,
 200 ppm
 500 ppm
 Replication ; 3
 Design ; RCBD
Evaluation Of Different
Provinance of Saussurea leppa
 Provinance ; Nellum Valley,
Leepa Valley and
Kaghan
Design; RCBD
To judge the effect of IAA on the
seed germination of Atropa
accuminta.
 Treatments ; Control, 500 ppm,
 1000 ppm
 Source ; Thandayani
 Replication ; 4
 Design ; RCBD
 Date Of Sowing ;
To judge the effect of G.A on
the seed germination of
Paeonia emodi
 Treatments ; Control, 200 ppm and
 300 ppm
 Source ; Mianadam
 Replication ; 4
 Design ; RCBD
 Date Of Sowing ;
Nursery of Paeonia emodi
Demonstration Plots
 Demonstration plots of Linum usitatisimum at
Akbar Pura, Medicinal Plant Farm Peshawar
and Haripur
Demonstration plots of Nigella sativa at
Akbar Pura, Medicinal Plant Farm Peshawar
and Haripur
Screening of the provenance of
Linum Seed
Date of sowing; 15-11-2012
Provenance; Peshawar, A.Pura
D.I.Khan
Replication; 3
Design; RCBD
Screening of the provenance of
Nigella sativa Seed
Date of sowing; 06-10-2012
Provenance; Peshawar, Swabi and
D.I.Khan
Replication; 3
Design; RCBD
Technology package
demonstration plot of Nigella
sativa
Date of sowing; 11-10-2012
Provenance; PFI Peshawar, A.Pura,
Harripur and NIFA
Replication; 3
Design; RCBD
Demo Plot of Nigella sativa
Production technology of
Foenicullum vulgare
 Plot size: 20m2
 Row to Row distance: 50cm
 Replication: 4
 Design: RCB
 Date of Sowing: 11 Oct, 2012
Demo Plot of Foenicullum
vulgare
Demonstration plot of Mulathi
Ex-situ conservation
 Nursery of Atropa accuminata, Saussurea
lappa, Podophylum emodi, Asparagus
adscendens, Saxifrage ciliate and
Fagopyrum esculentum raised in
Lathhouse at medicinal plant farm for
transplantation in Kuza Galli and Bhurban
In-Situ Conservation
KUZA GALLI FARM:
Fencing and Construction of Path
(Upstairs) to Farm
Kuza Gali
 Established conservation area of
Endangered medicinal plants
Demonstration Plots of
Podophylum emodi, Atropa
accuminata and Saxifraga ciliata at
Kuza Galli
Bhurban
 Demonstration Plots of Saxifrage ciliate
and Asparagus adscendens

Survey
 Drug market survey of medicinal plants of
Swat to asses the demand and supply.
 Ethnobotanical survey of Swat is under
progress.
 Ethnobotanical survey of Galliat forest
division is under progress.
Collection of Germ Plasm From
Swat For Cultivation
Germ Plasm
Germ Plasm
Anjibar Growing At Peshawar
Farm
Growing Nursery of Moringa
olefera
Tribulus terrestris (Markundi)
Peganum harmala (Spelanay)
Briefing to secretary
environment about activity of
the project
Pena flex For Public Awareness
ESTABLISMENT OF LATH
HOUSE
Establishment of QURANIC
garden
Publications
Publications
Publications
Publications
Publications
Descriptors of Variety Registeration
Descriptor of Variety Registeration of Flax
Thanks

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55. wwf final presentation A Presentation By Mr. Allah dad Khan Visiting Professor the University of Agriculture Peshawar allahdad52@gmail.com

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  • 2. CULTIVATION AND CONSERVATION OF MEDICINAL PLANTS THROUGH CUMMINITY PARTISIPATION CH. MUHAMMAD MUSLIM MEDICINAL PLANTS BOTANIST PAKISTAN FOREST INSTITUTE PESHAWAR
  • 3. OBJECTIVES  Qualitative & quantitative survey of medicinal plants & regeneration problems in the forest.  Cultivation trials of medicinal & aromatic plants for finding out agronomical data for improvements of crops.  To assess the demand & supply of herbal drug plants in the market.  To disseminate knowledge about the availability of medicinal plants to interested public & pharmaceutical industries.
  • 4. Someone once said there are only two types of fools: one professes “this is old and therefore good” and the other says “This is new and therefore better”  Point: Use your scientific evaluations in the matter, it is a matter of life or death!
  • 5. Use of Medicinal Plants  Use of medicinal plants developed from informal experimentation and based on a general familiarity with medicinal plants. This knowledge was amassed via experimentation over many generations and was handed down orally from person to person – often woman to woman in traditional cultures.
  • 7. Qualitative surveys 1950-1964 The medicinal plant branch carried out pioneer work on surveys of medicinal plants to determine medicinal plants potential in their natural habitats in different parts of country since its inception
  • 8. Survey of Baluchistan:1950-52 Survey of Kuram Agency:1952-54 Survey of Kalat:1954- 55
  • 9. Survey of Northern areas, Azad Jammu &Kashmir:1955-57 Survey of Tharparker:1957-58 Survey of Murree & Hazara Hill Forests:1958-62 Survey of Swat State:1963-64
  • 10. MEDICINAL PLANTS OF FOUR PROVINCES • Ephedra nebrodensis (Asmania), Juniperus excelsa (Abhel), Withania coagulans (Panir dodi), Thymus serphyllum (Ban ajwain), Prunus amygadalus (Badam talkh), Berberis lycium (Darhald), Glycyrrhiza glabra (Mulethi), Bunium persicum (Kala zeera), Perovskia abrotanoides (San shob) and Zuffa officinalis (Zufa) Baluchistan:
  • 11. Sindh Cassia angustifolia (Sanna), Ricinus communis (Castor), Commiphora mukul (Gugal), Citrulus colocynthis (Hantal), Cassia fistula (Amaltas) and Rhazya stricta (Vena)
  • 12. Punjab Fumaria indica (Shatra), Cassia absus (Chaksu), Adhatoda vasica (Berg bansa), Datura alba (Datura), Plantago ovata (Isabghol), Ricinus communis (Castor), Abutilon indicum (Pili buti), Solanum nigrum (Mako) and Peganum hermala (Hermal)
  • 13. Khyber Pakhtunkhwa Artemisia maritima (Afsanteine), Hyoscyamus niger (Ajwain khurasani), Dioscorea deltoidea (Kanis), Valeriana jatamansi (Mushk-bala), Berberis lycium (Darhald), Atropa acuminata (Angoor-i- shifa), Podophyllum hexandrum (Bankakri), Aconitum heterophyllum (Atees talkh), Bergenia ciliata (Zakham-i- hayat) and Viola sepens (Banafsha)
  • 14. NORTHERN AREAS AND AZAD KASHMIR Dioscorea deltoidea (Kanis), Saussurea costus (Kuth), Atropa acuminata (Angoor-i-shifa), Valeriana jatamansi (Mushk-bala), Aconitum heterophyllum (Atees talkh), Datura stramonium (Datura), Colchicum luteum (Suranjan-e-talkh), Ferula sp. (Hing), Rheum emodi (Revand chini), Podophyllum emodi(Bankakri), Viola serpens (Banafsha), Gentiana kurroo (Nilkant), Lavatera kashmiriana (Resha khatmi) and Swertia chirata (Chirata)
  • 15. Plants being used by various system of medicines
  • 16. Distribution of medicinal plants by habits
  • 17. Distribution of medicinal plants by families
  • 18. Break up of medicinal plant by their parts utilized
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  • 20. MANAGEMENT OF MEDICINAL AND AROMATIC PLANTS S # Group – A Group – B Long duration plants of higher elevations (2000– 3000m) Short duration plants of lower elevation and plains (Up to 1000m) 1. Dioscorea deltoidea (Kanis) Plantago ovata (Ispaghol) 2. Podophyllum emodi (Bankakri) Carum copticum (Ajwain) 3. Aconitum heterophyllum (Atis) Nigella sativa (Kalonji) 4. Valeriana wallichii (Mushkbala) Foeniculum vulgare (Sonf) 5. Berberis lycium (Darhald) Psoralea corylifolia (Babchi)
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  • 58. Atropa acuminata  Local name : Angoor-i-shifa  Family : Solanaceae  Status : Herb  Medicinal uses: The leaves and roots are used as sedative, antispasmodic and narcotic. The dried leaves are smoked as an antispasmodic. It is usually an important constituent of preparations rheumatism and inflammations as of the breast.
  • 62. Seed germination studies on Atropa acuminata at PFI, Peshawar Soil media Percentage germination Soil, sand & manure (33% each) 44 Soil & manure (50% each) 36 Sand & manure (50% each) 38 Source: Sharan (elevation 2800m) Exp. Design RCB Design Replications 3 5 gm sample 8 berries Average number of seed (5gm) 2081 Size of Petri dish 15cm diameter Seed sown in each Petri dish 100 Temperature 13-300C Date of sowing October 2003. Seed germination started after 40 days & took 27 days for complete germination
  • 63. Germination of treated & untreated seeds under field conditions  To increase the %age of germination & to reduce log time taken for germination, seeds were treated with 200ppm gibberellic acid for 30 minutes.  Seed started germination after 20 days as compared to untreated seeds which took 42 days. Treatment Date of sowing No. of days taken for initiation of germination No. of days taken for complete germination Germination % Untreated seed 5.10.2003 42 143 53 Seed soaked in 200ppm GA for 30 minutes 27.11.2003 22 98 93
  • 64. Experiments to test the effect of application of NPK (i) N 150+P60+K30 kg/ha (ii) N200+P90+K60 kg/ha & to determine the effect of various spacings (45x30, 45x45, 60x45, 60x60cm) on the growth, leaves & root yield of A. acuminata at kuzagali. Fertilizer having NPK in two combinations F1 N150+P60+K30 kg/ha F2 N200+P90+K60 kg/ha Spacings (cm) 45x30, 45x45, 60x45, 60x60 Exp. Design Split plot design Replications 4 Plot size 12m2 Total seedlings 1496 Date of sowing April, 2003
  • 65. Mean fresh leaves yield of A. acuminata (kg/plot 12m2) as affected by cultural & fertilizer trials Fertilizer treatment Spacing (cm) Fertilizer mean 45x30 45x45 60x45 60x60 F1 0.67 0.57 0.58 0.34 0.54 F2 0.79 0.65 0.60 0.46 0.62 Spacing mean 0.75 0.61 0.56 0.40 Results indicated that plants spaced at 45x30 &45x45cm resulted in a significant increase in yeild of leaves (2.79 & 2.64 kg/plot) or 2.33 & 2.20 tonnes/ha per annum respectively as compared to wider spacings 60x45 & 60x60cm (2.10 & 1.94 kg/plot) or 1.75 & 1.62 tonnes/ha per annum. Application of split doses of NPK (N200, P90 & K60 kg/ha) gave higher yield of fresh leaves (2.49kg/plot) or 2.08 tonnes/ha per annum as compared to lower doses of NPK fertilizer, i.e., N150, P60 &K30 kg/ha(2.24 kg/plot) or 1.87 tonnes/ha per annum.
  • 66. Mean fresh root yield (kg/plot) as affected by various spacing & fertilizer treatments Fertilizer treatment Spacing (cm) Fertilizer mean 45x30 45x45 60x45 60x60 F1 3.38 2.82 2.41 2.26 2.72 F2 3.75 3.15 2.81 2.46 3.04 Spacing mean 3.57 2.98 2.61 2.36 Yield results indicated that higher root yield was obtained in plants spaced at 45x30cm as compare to other spacing. Application of split doses of NPK (N200, P90, K60) gave higher yield of roots as compared to lower doses of NPK (N150, P60, K30).
  • 67. Recommendations  Fresh leaves yield was low during first year but it increased with the duration of time from second to third year growth. Root took 3 years growth period to attain commercial size for harvesting & utilization.  The regeneration percentage of A. acuminata plant ranged from 40-66% as observed in various experiments.
  • 68. Colchicum luteum  Local name : Suranjan-talkh  Family : Colchicaceae  Status : Herb  Medicinal uses: The corms are used as carminative, laxative and aphrodisiac. It is also given in gout, diseases of liver and spleen. The corms are also employed as an external application for lessening inflammation and pain.
  • 71. Effect of planting different portions of corms (Daughter, Mother & Full corms) of Colchicum luteum on the regeneration of corms at Peshawar. Treatments  Source: Abbottabad  Portion of corm: Daughter, Mother & Full corm  Mode of planting: Ridges  Plot size: 32m2  Plant to Plant distance: 15cm  Row to Row distance: 30cm  Replications: 5  Number of rows: 10  Layout: RCB Design  Date of sowing: 26thFebruary, 2004.
  • 72. Mean sprouting percentage in T1 (Daughter) 103.2, T2 (Full) 73.4 and T3 (Mother) 60.0 was recorded respectively. Highly significant differences were observed in mean sprouting of treatments T1 (Daughter), T2 (Full) and T3 (Mother) respectively. Therefore T1 (Daughter) corms are more suitable for cultivation of corms of C. luteum. Regeneration of Colchicum luteum (sprouts out of 250 corms) recorded on February 28, 2004. Replications T1 (Daughter) T2 (Full) T3 (Mother) Total R1 109 80 55 244 R2 120 63 59 242 R3 105 75 65 245 R4 89 70 59 218 R5 93 79 62 234 Total 516 367 300 1183 Mean 103.2 73.4 60.0 Sprouting % 41.28 29.36 24.0
  • 73. Dioscorea deltoidea  Local name : Kanis, kildri  Family : Dioscoreaceae  Status : Herb  Medicinal uses:  The tubers are employed in the treatment of bilious colic and as a diuretic and expectorant. They are also used to kill lice and fish poison. The tubers are a very good source of diosgenin which is a starting material for hormone preparation.
  • 77. Comparative studies on the performance of Dioscorea deltoidea rhizomes collected from different ecological zones i.e., Kaghan, Murree & Galliat areas for their growth & yield of rhizomes. Source: Murree, Galliat & Kaghan Plat size: 30m2 Experimental Design: RCB Replications: 6 Plant to plant distance: 30 cm Row to row distance: 45 cm Rhizome weight: 25gm Date of sowing: 2000-06
  • 78. Comparative studies on the performance of Dioscorea deltoidea rhizomes collected from different ecological zones i.e., Kaghan, Murree & Galliat areas for their growth & yield of rhizomes. Source 3 Years growth 6 Years growth Galliat 6.60 8.54 Murree 6.27 8.52 Kaghan 5.45 7.62 Comparative mean yield of D. deltoidea (kg/plot) Yield results indicate that Galliat source gave higher rhizome yield (8.54 kg/plot) followed by Murree (8.52 kg/plot) & Kaghan (7.62 kg/plot) after 6 years growth. These results showed yield result of 3 years and yield of rhizomes increased with duration of time.
  • 79. Recommendations  Galliat source gave better regeneration percentage and higher yield of rhizomes as compared to other sources and is suitable for planting for regeneration purposes.
  • 80. Podophyllum emodi  Local name : Bankakri  Family : Berberidaceae  Status : Small erect herb  Medicinal uses:  The seed are demulcent, stimulant and nervine tonic. The oil is slightly laxative. The cake left after extraction of the fixed oil is used in cases of diabetes. Burnt shell of almond is used as a tooth-powder.
  • 85. Studies to judge the effect of planting different aged rhizomes (1 year, 2 years, 3 years & full) on sprouting, growth & yield of P. emodi rhizomes harvested after 4 years growth at Thandiani. Source: Thandiani Treatments 1 year, 2 years, 3 years & full Plot size 6m2 Exp. Design Split plot Design Replications 4 Total rhizomes planted 39 Spacing 30x60cm Date of sowing October 2002.
  • 86. Ages of rhizomes planted (in years) Replications Mean 1 2 3 4 1 0.49 0.46 0.41 0.45 0.45 2 0.56 0.54 0.50 0.46 0.51 3 0.73 0.65 1.52 0.64 0.63 Full 0.77 0.74 0.61 0.68 0.69 Mean fresh yield of rhizomes of P. emodi as affected by planting various aged rhizomes harvested after 4 years growth (kg/plot)
  • 87. Recommendations  Planting of 2 bud portion (2 years old stock) gave large number of propagule then planting of full, 1 & 3 years rhizomes.  Two buds portion would be economical to provide more number of plants and higher yield can be obtained after 8-10 years growth.  Podophyllum rhizomes are slow growing and takes 8-10 years for attaining commercial size for harvesting, therefore, a rotation period of 10 years is recommended for exploitation of rhizomes.
  • 88. Saussurea lappa  Local name : Kuth  Family : Compositae  Status : Herb  Medicinal uses: Dried roots are aromatic, tonic, stomachic, carminative, stimulant and useful in asthma, cough, fever, chest problems and cholera. It is also used skin diseases and rheumatism. It has also antiseptic and disinfectant properties. The root has been much priced as an aromatic substance.
  • 92. Germination studies on Saussurea lappa at PFI, Peshawar. Source: Azad Kashmir Exp. Design RCB Design Replications 4 Ten random samples 5gm each 5gm (number of seeds 336) Two samples treated 200ppm, 100ppm GA (30 minutes) Third sample Chilling at 0oC (48 hours) Fourth sample Control Total tubes sown 1600 Date of sowing January 2000.
  • 93. Seed germination of S. lappa in various treatments under field conditions Treatment No. of days taken for initiation of germination No. of days taken for complete germination Seed germination % Control 35 65 60 Chilling 21 50 89 200ppm GA 14 40 97 100ppm GA 14 40 91 Maximum germination (97%) was obtained with 200ppm GA followed by 100ppm (91%) & chilling treatment (89%) against control (60).GA treatment also reduced germination initiation time to 14 days as compared to control which took 35 days. Similarly chilling also reduced initiation time to 21 days.
  • 94. Introduction & adaptability studies on S. lappa to judge the effect of various spacings on the growth & root yield harvested after 3 & 5 years growth at Thandiani. Source: Shardah Forest Division, AJK Exp. Design RCB Design Replications 4 Plot size 15m2 (16 plots) Total seedlings planted 1200 Date of sowing April, 2000 Treatment Spacing (cm) 2003 3 years growth 2005 5 years growth 45x30 0.84 1.49 45x45 0.58 1.01 60x30 0.76 1.27 60x60 0.47 0.78 Mean 0.66 1.14 Mean fresh yield of S. lappa (kg/plot) as affected by various spacing treatments
  • 95. Recommendations  S. lappa plants being perennial requires at least 5 years growth period for development of good size roots for commercial exploitation, therefore a rotation of five years may be fixed for its harvesting and better regeneration in its natural habitat.  A spacing of 60x30cm is suitable for artificial planting in areas where the plants are scarce.
  • 96. 2. Study of medicinal plants among weeds of wheat and maize in Peshawar region. Objectives:  To identify the weeds of medicinal importance.  To determine the main constituents & beneficial importance of weeds. Study Period: 2010-12 Locations of study:  Palosi (Peshawar)  Mardan  Swabi  The study is under progress. 1. Survey of drug markets of Peshawar.
  • 97. Silybum marianum Family: Asteraceae English name: Milk thistle Locality: Wheat field of Malakandher Part used: Whole herb root, leaves, seeds. Medicinal value: Seeds are used in jaundice.
  • 99. Melilotus indica Family: Fabaceae Common names:Senji (Urdu), Levanai (Pushto), India clover (English) Locality: Wheat Field Malakandher Part used: Whole plant, Seeds Medicinal value: Externally applied as poultice or plaster for swellings. Seeds are used in bowel complaints and diarrhea. Local uses: Used against skin diseases.
  • 101. Euphorbia helioscopia Family: Euphorbiaceae Common names:Mandaroo (Pushto), Zahar booti (Punjabi), Cat's milk (English.) Locality: Wheat field Malakandher Part used: Shoot, leaf, root. Medicinal value: Seeds with pepper given in cholera. Milky juice applied to eruptions. Local uses: Used in skin diseases.
  • 103. Fumaria indica Family: Fumariaceae Names: Papra (Pushto), Shahtra (Urdu), Fumitory (English) Locality: Wheat field of Malakander. Part used: Whole plant. Medicinal value: Plant is used as aperients, diaphoretic and diuretic.
  • 105. Production of Medicinal Herbs in Collaboration with Private Sector (PMHPS) Project. Duration: Five years (60 months) Commencement date: 1st July 2006 Termination date: 30th June 2011 Sponsoring agency: MinFAL, Islamabad Staff: Research Assistant (1) Forester (1) Driver (1) Objectives:  To develop research based package of agro-technology to comply WHO guidelines on Good Agriculture, Collection and Processing Practices (GACP).  Multiplication of registered seed and propagation materials on farmer’s field.  Promotion of commercially important medicinal herbs in earthquake hit areas.  Survey of crude herbal drug markets of Pakistan to determine supply and demand position of crude drugs of vegetable origin.
  • 106. Cultivation of important economic and medicinal plants Name of crops Sowing time Germination time R to R spacing (cm) Estimated flowering time Harvesting time Estimated Yield/ ha. (kg) Income Rs.000 Nigella sativa (Kalongi) October 15 days 30 March April-May 550-650 60-70 Linum Usitatissimum (Alsi) October November 8 days 30 February -do- 700-800 30-40 Carum copticum (Ajwain) October 12 days 36 March May 500-550 30-35 Foeniculum vulgare (Sonf) October 8-10 days 30-45 March April-May 800-900 50-60 Lallementia royleana (Tukhm-i-balanga) September October 10-12 days 30 February April 450-600 70-80 Plantago ovata (Isabghol) September October 6 days 15-20 February March- April 450-500 40-45
  • 107. Studies to test the effect of various row-to-row spacing on the seed yield of Carum copticum at PFI, Peshawar. Objectives: Standardization of suitable row-to-row spacing for getting higher seed yield. Material and methods: Seed source: Peshawar  Plot size: 20 m2  Row to row distance: Broadcasting, 24, 36 and 48 cm  Replications: 4  Layout: RCB Design  Date of sowing: 14th October, 2008.
  • 108. Row to row spacing (cm) BC 24 36 48 0.561 0.794 1.246 0.866 0.403 0.545 0.610 0.489 0.231 0.325 0.375 0.374 0.245 0.218 0.318 0.324 Mean 0.360 0.470 0.637 0.513 Results achieved indicated that row-to-row spacing of 36 cm resulted in a significant increase in the seed yield (318.5 kg/ac) as compared to 48, 24 cm and broadcasting (256, 235, 180 kg/ac) respectively. No significant difference was observed in the mean seed yield of 48 and 24cm row to row spacing and both spacing proved superior over broadcasting.
  • 109. Bio-Trace spray on Carum copticum at PFI, Farm
  • 110. Fertilizer trials on Nigella sativa crop at PFI, Peshawar. Objectives:  Determination of appropriate dose of nitrophos fertilizer in order to get optimum seed yield. Material and methods: Seed source: Peshawar. Plot size: 20 m2 Row-to-Row distance: 30cm Replications: 4 Layout: RCB Design Date of sowing: 4th October 2008. Number of rows: 12 Doses of Nitrophos fertilizer (kg/ac): 0, 50, 75 and 100.
  • 111. Doses of nitrophos fertilizer (kg/plot) 0 50 75 100 1.736 2.534 3.123 3.012 0.643 1.990 2.128 2.420 Mean 1.189 2.262 2.625 2.716 The application of split doses of nitrophos fertilizer @ 100 kg/ac gave higher seed yield (1099 kg/ac) as compared to 75 kg nitrophos fertilizer/ac (1062 kg/ac) and control (481.37 kg/ac). There was no significant difference in the mean seed yield of 75 and 100 kg nitrophos/ac as both doses were mutually alike.
  • 113.
  • 114. Cultural and fertilizer trials on Linum usitatissimum at PFI, Peshawar Objectives:  To find out water requirement (6 v 3 irrigation) and application of different doses of nitrophos fertilizer on the seed yield. Materials and methods: Seed source: PFI, Farm Plot size: 30 m2 Row to row distance: 30 cm Replications: 4 Layout: split plot design Date of sowing: 9th October 2008 Number of rows: 10 Irrigation interval: fortnightly vs. 3 weeks Doses of nitrophos Fertilizer (Kg/ac): 0, 50, 100 and 150 kg
  • 115. Doses of nitrophos fertilizer (kg/plot) Irrigation number 0 50 100 150 6 0.836 1.300 1.600 1.340 3 0.673 0.793 0.873 0.825 Pooled mean for fertilizer 0.754 1.046 1.236 1.082 Linum crop showed good response to the higher number of irrigations 6 (fortnightly) as compared to 3 irrigation (3 weeks). The application of split doses of nitrophos fertilizer @100 kg/ac gave higher seed yield (500.40 kg/ac) as compared to 50 kg nitrophos fertilizer/ ac (423.48 kg/ac) and control (305.26 kg/ac). There was no significant difference in the mean seed yield of 100 and 150 kg nitrophos/ac as both doses were mutually alike.
  • 116.
  • 117. Experimental plot of Linum usitatissimum at PFI, Farm
  • 118.
  • 119. Fertilizer trials on Foeniculum vulgare crop at (Bachai) Swabi Objectives:  Determination of appropriate dose of nitrophos fertilizer in order to get optimum seed yield. Material and methods: Seed source: Bara. Plot size: 20m2 Row-to-Row distance: 45cm Replications: 4 Layout: RCBD Date of sowing: 18th October 2008. Number of rows: 10 Doses of Nitrophos fertilizer (kg/ac): 0, 50, 75 and 100.
  • 120. Cultivation of sonf at Bachai (Swabi)
  • 122. Foenicullum vulgare (sonf) plot at Bachai (Swabi)
  • 123. Cultivation and yield trials of Matricaria chamomilla (gul-i- baboona) to standardize its cultivation technology Objectives: To standardize its cultural requirement and economic feasibility under Peshawar climatic conditions Material and methods: Seed source: Swat Plot size: 20m2 Replications: 4 Layout: RCBD Plant to plant distance: 4cm Date of sowing: 20th October 2008. Number of rows: 10 Treatment: (20, 30, 40 cm and conventional broadcasting)
  • 124. Effect of various row to row spacing on the flower yield in kg/plot (20 m2). Row spacing (cm) Replication Broadcasting 20 30 40 1 2.000 4.000 5.800 1.800 2 2.010 5.200 5.000 2.000 3 1.990 2.900 4.120 5.010 4 2.220 4.400 6.000 4.000 Mean 2.055 4.125 5.230 3.202 A row to row spacing of 30 cm gave higher yield of fresh flower heads (5.23) as compared to broadcasting (2.05), 40 spacing cm (3.20) and 20 cm spacing (4.12) kg/plot respectively.
  • 127. Introduction of Glycyrrhiza glabra plantation at Medicinal Plants Farm, PFI, Peshawar. Common name: Mulathi Glycyrrhiza glabra (Mulaithi) roots were collected from Mastooj and garam chashma (Chitral) during 2005-06. Six hundred root cuttings were cultivated at Medicinal Plants Farm, Peshawar in September 2005-06. Part used: Roots. Area: 1 Kanal Uses: Expectorant, cough, jaundice, kidney stones, water-extract contains glycyrrhizic acid, glycyrrhizin, useful in anti-allergic diseases. 50 times sweeter than cane sugar.
  • 128. Glycyrrhiza glabra at PFI, Peshawar
  • 130. Studies to compare the yield of two strains of turmeric (Curcuma domestica) at Mardan. Common name: Haldi Area: 4 Kanal Part used: Rhizome. Uses: Anti-Cancer, Anti-HIV, healing property, Hypo- cholestrolmic, Diabetes, Carminative, aromatic, tonic, blood purifier.
  • 131. Studies to compare the yield of two strains of turmeric (Curcuma domestica) at Mardan Strains of turmeric:  Bannu  Changa-Manga Plat size: 34m2 Experimental Design: LSD Replications: Four Plant to plant distance: 25 cm Row to row distance: 30 cm Rhizome size: 4-5 cm Date of sowing: March, 2009.
  • 132. Studies to compare the yield of two strains of turmeric (Curcuma domestica) at Mardan Strains Yield (Kg/ha) Bannu 4150 Changa-Manga 4370  5 kg rhizomes of turmeric were given for chemical analysis to Dr. Javed Abbas Bangash of PCSIR laboratories, Peshawar.
  • 133. Performance of Curcuma domestica (Haldi) at Mardan
  • 136. Performance of Curcuma domestica (Haldi) at Mardan
  • 140. Comparative study of two sources of Rosa damascena for flower production at PFI, Peshawar. Rosa damascena (Desi gulab) Source:  Kalar Kahar  Peshawar Plot size: 48m2 Row to row distance: 1m Plant to plant distance: 30cm Date of sowing: 25-01-2010 Number of cuttings/plot: 70 Total number of cuttings: 560 Experimental design: LSD
  • 142. Studies to test the effect of different doses of nitrophos fertilizer on the growth and seed yield of Plantago ovata at D.I.Khan Objectives:  Determination of appropriate dose of nitrophos fertilizer in order to get optimum seed yield. Material and methods: Seed source: D.I.Khan Plot size: 20m2 Row-to-Row distance: 45cm Replications: 5 Layout: RCBD Date of sowing: 18th October 2008. Number of rows: 10 Doses of Nitrophos fertilizer (kg/ac): 0, 50, 75 and 100.
  • 143. Effect of different doses of nitrophos fertilizer on seed yield of Plantago ovata Doses of nitrophos (kg/ac) Mean yield in kg/ac 0 176 50 195 75 259 100 268 Application of split doses of nitrophos at the rate of 100 and 75 kg/ha resulted in a significant increase in the seed yield (268 and 259 kg/ac) respectively as compared to 50 kg nitrophos/ac (195 kg/ac) and control treatments (176 kg/ac).
  • 146. Survey of Plantago ovata (isabghol) in District Bhawalpur  Survey of Plantago ovata (Isabghol) cultivation, harvesting, processing and trading was carried out in Haroonabad, Hasilpur, Cheshtian, Dharanwala and Multan under the direction of Dr. Muhammad Aslam Gill, Project Director, PMHPS Project.
  • 147. Land holding capacity of farmers who are growing Plantago ovata S # Total area (Acres) Number of farmers in this group 1 25-50 10% 2 11-20 15% 3 5-10 75% From the data in the above table, it is evident that land holding size (5-10) is the largest (75%). Tenure status of sampled farms Stratum Owner (%) Tenant (%) Tenant-cum-owner All areas 93.9 5.8 0.3 93.9% of the sampled farms are owner operated, 5.8% tenant operated and the remaining 0.3% are the tenant-cum-owner operated.
  • 154.
  • 155. Constraints associated with the processing of medicinal plants  Poor agricultural practices  Poor harvesting and post-harvest treatment practices  Lack of research on development of high-yielding varieties, domestication etc.  Poor propagation methods  Inefficient processing techniques leading to low yields and poor quality products  Poor quality control procedures  High energy losses due to processing  Lack of current good manufacturing practices  Difficulties in marketing  Lack of local market for primary processed products  Lack of trained personnel and equipments  Lack of facilities to fabricate equipment locally  Lack of access to latest technologies and market information
  • 156.
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  • 169. Linum & Nigella sativa at D.I.Khan
  • 172. PAEONIA EMODI (Mamekh) ATROPA ACUMINATA (Angoor-i-shifa)
  • 173. ii. Vulnerable species Berberis lycium (Kashmal) Corydalis govaiana (Mamiri) Bergenia ciliata (Zakham-i-Hayat) Jurnea macrocephala (Gugal dhoop) Heracleum candicans (Kamur) iii. Extinct species Elaeganus hortensis (Singli) Saussurea costus (Kuth) S. sacra (Jogibadshah) Onasma bracteatum (Gaozaban) Corydalis govaiana (Mamiri)
  • 174. iv. Rare species Achilles mille folium (Monandu) Aconitum heterophyllum (Atees) A. chasmenthum (Patrees) Lavetera kashmiriana (Resha khatmi) Rheum emodi (Revand chini) Viola serpens (Banafsha) Swertia chirata (Chirata) Angelica glauca (Chora) Zanthoxylum armatum (Timar) Achilles millefolium (Monandu)
  • 180. Digitalis lanata and D. purpurea
  • 183. Trees of medicinal value Emblica officinalis (Amla) Sapindus mukorrossi (Ritha)
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  • 202.
  • 203. WHAT MEASURES CAN BE DONE FOR THE CONSERVATION OF MEDICINAL PLANTS???? • Law Enforcement to Protect The Forest  • Reforestation  • Rangeland Management  • Land Reforms  • Awareness Raising  • Capacity building of local people  – Collection, Cultivation, Processing and  Marketing of Medicinal Plants  • Linkage of collectors with Market  – Industries (Pharmaceutical and Herbal)  Dealers
  • 204. Seed Collection  Seeds of Atropa accuminata, Saussurea lappa, Podophylum emodi, Asparagus adscendens, Saxifrage ciliate and Fagopyrum esculentum were collected from Swat, Gilgit Baltistan, Neelam Valley and Jehlum Valley
  • 205. To Judge The Effect Of GA On The Seed Germination Of Saussurea lappa,  Date of Sowing;  Treatment ; Control,  200 ppm  500 ppm  Replication ; 3  Design ; RCBD
  • 206.
  • 207. Evaluation Of Different Provinance of Saussurea leppa  Provinance ; Nellum Valley, Leepa Valley and Kaghan Design; RCBD
  • 208. To judge the effect of IAA on the seed germination of Atropa accuminta.  Treatments ; Control, 500 ppm,  1000 ppm  Source ; Thandayani  Replication ; 4  Design ; RCBD  Date Of Sowing ;
  • 209.
  • 210. To judge the effect of G.A on the seed germination of Paeonia emodi  Treatments ; Control, 200 ppm and  300 ppm  Source ; Mianadam  Replication ; 4  Design ; RCBD  Date Of Sowing ;
  • 212. Demonstration Plots  Demonstration plots of Linum usitatisimum at Akbar Pura, Medicinal Plant Farm Peshawar and Haripur
  • 213. Demonstration plots of Nigella sativa at Akbar Pura, Medicinal Plant Farm Peshawar and Haripur
  • 214. Screening of the provenance of Linum Seed Date of sowing; 15-11-2012 Provenance; Peshawar, A.Pura D.I.Khan Replication; 3 Design; RCBD
  • 215.
  • 216. Screening of the provenance of Nigella sativa Seed Date of sowing; 06-10-2012 Provenance; Peshawar, Swabi and D.I.Khan Replication; 3 Design; RCBD
  • 217. Technology package demonstration plot of Nigella sativa Date of sowing; 11-10-2012 Provenance; PFI Peshawar, A.Pura, Harripur and NIFA Replication; 3 Design; RCBD
  • 218. Demo Plot of Nigella sativa
  • 219. Production technology of Foenicullum vulgare  Plot size: 20m2  Row to Row distance: 50cm  Replication: 4  Design: RCB  Date of Sowing: 11 Oct, 2012
  • 220. Demo Plot of Foenicullum vulgare
  • 222. Ex-situ conservation  Nursery of Atropa accuminata, Saussurea lappa, Podophylum emodi, Asparagus adscendens, Saxifrage ciliate and Fagopyrum esculentum raised in Lathhouse at medicinal plant farm for transplantation in Kuza Galli and Bhurban
  • 223.
  • 224. In-Situ Conservation KUZA GALLI FARM: Fencing and Construction of Path (Upstairs) to Farm
  • 225.
  • 226. Kuza Gali  Established conservation area of Endangered medicinal plants
  • 227. Demonstration Plots of Podophylum emodi, Atropa accuminata and Saxifraga ciliata at Kuza Galli
  • 228. Bhurban  Demonstration Plots of Saxifrage ciliate and Asparagus adscendens 
  • 229. Survey  Drug market survey of medicinal plants of Swat to asses the demand and supply.  Ethnobotanical survey of Swat is under progress.  Ethnobotanical survey of Galliat forest division is under progress.
  • 230.
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  • 233. Collection of Germ Plasm From Swat For Cultivation
  • 236. Anjibar Growing At Peshawar Farm
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  • 240. Growing Nursery of Moringa olefera
  • 241.
  • 244. Briefing to secretary environment about activity of the project
  • 245.
  • 246. Pena flex For Public Awareness
  • 254. Descriptors of Variety Registeration
  • 255. Descriptor of Variety Registeration of Flax
  • 256.
  • 257. Thanks