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Diagnostic ultrasonography in demestic
animal reproduction
By: Ali Hussain
Definition
 High frequency Sound waves inaudible to
human ear (20-20000 Hz)
Ultrasound applications
■ Cardiac imaging
■ Abdominal organs imaging
■ In-utero imaging of the developing fetus
■ Cancer imaging
■ Musculoskeletal imaging
■ Ophthalmology
■ Doppler - Doppler ultrasonography is an
additional technique used to identify blood
flow and velocity
Basic Principle
 The ultrasound equipment basically
consists of a transducer and a scan
converter.
 The transducer is the ultrasound producing
part.
 It is fitted with a piezoelectric crystal (Lead
– zirconate – titanate or others) which when
stimulated by a high voltage current emits
the ultrasound.
Ultrasound Machines
 The ultrasound is transmitted to the patient
from the transducer and propagates
through the tissues.
 The ultrasound beam is either reflected
back, partially absorbed or entirely
absorbed. The returning beam (echoes)
meets back and deforms the crystals in
the transducer.
 This mechanical energy is converted back
to an electrical signal proportional to the
strength of the echo and delayed by a time
roughly proportional to the distance
traveled.
 The scan converter interprets the
variations in brightness displayed on the
cathode ray tube of a B-mode system (or
as a variation in amplitude in A-mode
oscilloscope screen) and also stores
images when required.
 The ultrasound is emitted in a pulse –
echo manner. A pulse of ultrasound is
emitted, and its reflection perceived prior
to emission of the next pulse.
Types of instruments
 For most diagnostic veterinary purposes B-
mode, real time ultrasonography is used
employing different types of transducers.
 Linear transrectal transducer (frequencies of
5-10 MHz ) and the Sector trans-abdominal
transducer (frequencies of 1.0-4.0 MHz ). For
most reproductive diagnostic work, linear
array transrectal transducers are employed
in cattle, buffaloes, mares and female
camels.
Images by a sector and linear
probe
 A- mode Amplitude modulation. A one-
element (one dimensional) display with
time (distance) on the horizontal axis.
 B-Mode Brightness modulation. A
compound A-mode scan with amplitude
translated into a brightness scale. Location
on the display is related to position and
depth.
 Doppler ultrasound When an ultrasound
beam meets a moving object the reflected
ultrasound is either of increased or
decreased frequency, depending upon
whether the motion is towards or away
from the transducer.
 Echogenic A structure causing a marked
reflection of the ultrasound beam. A
change in echogenecity in a
homogeneous structure may indicate a
pathological change.
 Hyperechoic Showing increased
echogenicity.
 Hypoechoic Showing decreased
echogenicity.
 Attenuation in ultrasound is the reduction
in amplitude of the ultrasound beam as a
function of distance through the imaging
medium
Ultrasound artifacts
 An ultrasound artifact is a structure in an
image which does not directly represent
actual tissue being scanned.
 Artifact assumes different forms including :
• Structures in the image that are not
actually present
 Objects that should be represented but are
missing from the image.
Common artifacts in reproductive
ultrasound
 Artifacts in ultrasound may be divided into
two main categories:
 (1) artifacts related to the operator, such
as wrong settings (power, gain, frequency)
or poor patient preparation, which impair
image quality;
 (2) artifacts that result from ultrasound
interactions with tissues (absorption,
reflection or refraction). If adequately
identified, they may be useful in the
characterization of a lesion.
Reverberation
 This is the production of false echoes due
to repeated reflections between two
interfaces with a high acoustic impedance
mismatch
 The air between the probe and the skin is
the main cause of reverberation (external
reverberation).
 Reflectors such as intestinal gas and
bones are causes for internal
reverberation. This artifact is characterized
by formation of several hyperechoic lines
that are equally spaced and gradually
attenuated
 Gas within the spiral
colon is creating
reverberation artifacts
(white arrowheads)
characterized by
several hyperechoic
lines, equally spaced
and gradually
attenuated.
Reverberation
COMET TAIL ARTIFACT
 The comet-tail artifact is a type of
reverberation artifact met with small
reflective surfaces as gas bubbles or small
metallic objects and is characterized by
the formation of a narrow beam of closely
spaced, discrete, hyperechoic lines
Reproductive diagnostics
 Evaluation of ovarian and uterine
physiology and pathology
 Ovarian follicle, CL, Cysts, tumors, etc..
 Studying ovarian follicular dynamics
 Varian blood supply by color doppler
 Normal uterine echotexture, estrus
 Growths or fluid accumulations such as
mucometra, pyometra, tumors
Evaluation of pregnancy &
gestational physiology
 Pregnancy diagnosis
 Fetal heartbeat
 Fetal sex determination
 Determination of gestational age
 Fetal numbers and viability
 Estimating abnormal pregnancies
 Fetal growth assessments
 Early embryonic losses
Interventions
 Ultrasound guided ovum pick-up for IVF
 Ultrasound guided aspiration of fetal
contents of one fetus in twin pregnancies
 Aspiration of amniotic or fetal fluids
Patient preparation
 For transrectal examination the probe is
placed in a sleeve or condom with gel
inside.
 The animal is restrained, and the rectum is
evacuated from the feces.
 The probe is inserted in the rectum and
the ovaries and uterus are scanned
 For every transcutaneous
ultrasonographic examination, the area
has to be shaved and the skin cleaned
with water or alcohol. Acoustic gel is then
applied to improve the contact between
the probe and the skin.
Thank you

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M-2 2018-ag-7688 Diagnostic ultrasonography in domestic animal reproduction.ppt

  • 1. Diagnostic ultrasonography in demestic animal reproduction By: Ali Hussain
  • 2. Definition  High frequency Sound waves inaudible to human ear (20-20000 Hz)
  • 3. Ultrasound applications ■ Cardiac imaging ■ Abdominal organs imaging ■ In-utero imaging of the developing fetus ■ Cancer imaging ■ Musculoskeletal imaging ■ Ophthalmology ■ Doppler - Doppler ultrasonography is an additional technique used to identify blood flow and velocity
  • 4. Basic Principle  The ultrasound equipment basically consists of a transducer and a scan converter.  The transducer is the ultrasound producing part.  It is fitted with a piezoelectric crystal (Lead – zirconate – titanate or others) which when stimulated by a high voltage current emits the ultrasound.
  • 6.
  • 7.  The ultrasound is transmitted to the patient from the transducer and propagates through the tissues.  The ultrasound beam is either reflected back, partially absorbed or entirely absorbed. The returning beam (echoes) meets back and deforms the crystals in the transducer.
  • 8.  This mechanical energy is converted back to an electrical signal proportional to the strength of the echo and delayed by a time roughly proportional to the distance traveled.  The scan converter interprets the variations in brightness displayed on the cathode ray tube of a B-mode system (or as a variation in amplitude in A-mode oscilloscope screen) and also stores images when required.
  • 9.  The ultrasound is emitted in a pulse – echo manner. A pulse of ultrasound is emitted, and its reflection perceived prior to emission of the next pulse.
  • 10.
  • 11. Types of instruments  For most diagnostic veterinary purposes B- mode, real time ultrasonography is used employing different types of transducers.  Linear transrectal transducer (frequencies of 5-10 MHz ) and the Sector trans-abdominal transducer (frequencies of 1.0-4.0 MHz ). For most reproductive diagnostic work, linear array transrectal transducers are employed in cattle, buffaloes, mares and female camels.
  • 12. Images by a sector and linear probe
  • 13.  A- mode Amplitude modulation. A one- element (one dimensional) display with time (distance) on the horizontal axis.  B-Mode Brightness modulation. A compound A-mode scan with amplitude translated into a brightness scale. Location on the display is related to position and depth.
  • 14.  Doppler ultrasound When an ultrasound beam meets a moving object the reflected ultrasound is either of increased or decreased frequency, depending upon whether the motion is towards or away from the transducer.  Echogenic A structure causing a marked reflection of the ultrasound beam. A change in echogenecity in a homogeneous structure may indicate a pathological change.
  • 15.  Hyperechoic Showing increased echogenicity.  Hypoechoic Showing decreased echogenicity.  Attenuation in ultrasound is the reduction in amplitude of the ultrasound beam as a function of distance through the imaging medium
  • 16. Ultrasound artifacts  An ultrasound artifact is a structure in an image which does not directly represent actual tissue being scanned.  Artifact assumes different forms including : • Structures in the image that are not actually present  Objects that should be represented but are missing from the image.
  • 17. Common artifacts in reproductive ultrasound  Artifacts in ultrasound may be divided into two main categories:  (1) artifacts related to the operator, such as wrong settings (power, gain, frequency) or poor patient preparation, which impair image quality;
  • 18.  (2) artifacts that result from ultrasound interactions with tissues (absorption, reflection or refraction). If adequately identified, they may be useful in the characterization of a lesion.
  • 19. Reverberation  This is the production of false echoes due to repeated reflections between two interfaces with a high acoustic impedance mismatch  The air between the probe and the skin is the main cause of reverberation (external reverberation).
  • 20.  Reflectors such as intestinal gas and bones are causes for internal reverberation. This artifact is characterized by formation of several hyperechoic lines that are equally spaced and gradually attenuated
  • 21.  Gas within the spiral colon is creating reverberation artifacts (white arrowheads) characterized by several hyperechoic lines, equally spaced and gradually attenuated.
  • 23. COMET TAIL ARTIFACT  The comet-tail artifact is a type of reverberation artifact met with small reflective surfaces as gas bubbles or small metallic objects and is characterized by the formation of a narrow beam of closely spaced, discrete, hyperechoic lines
  • 24. Reproductive diagnostics  Evaluation of ovarian and uterine physiology and pathology  Ovarian follicle, CL, Cysts, tumors, etc..  Studying ovarian follicular dynamics  Varian blood supply by color doppler  Normal uterine echotexture, estrus  Growths or fluid accumulations such as mucometra, pyometra, tumors
  • 25. Evaluation of pregnancy & gestational physiology  Pregnancy diagnosis  Fetal heartbeat  Fetal sex determination  Determination of gestational age  Fetal numbers and viability  Estimating abnormal pregnancies  Fetal growth assessments  Early embryonic losses
  • 26. Interventions  Ultrasound guided ovum pick-up for IVF  Ultrasound guided aspiration of fetal contents of one fetus in twin pregnancies  Aspiration of amniotic or fetal fluids
  • 27. Patient preparation  For transrectal examination the probe is placed in a sleeve or condom with gel inside.  The animal is restrained, and the rectum is evacuated from the feces.  The probe is inserted in the rectum and the ovaries and uterus are scanned
  • 28.  For every transcutaneous ultrasonographic examination, the area has to be shaved and the skin cleaned with water or alcohol. Acoustic gel is then applied to improve the contact between the probe and the skin.