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Journal of Biology, Agriculture and Healthcare www.iiste.org
ISSN 2224-3208 (Paper) ISSN 2225-093X (Online)
Vol.4, No.24, 2014
167
Effect of Ash and Sawdust of Shear Butter) (Vitellaria paradoxa )
Storage Treatments on The Tuber Nutrient of Some Sweet Potato
[Ipomoea batatas (L.) Lam.] Cultivars
F.S Jwakdak 1*
& C.O Akueshi 2
1. Department of Biology, College of Education, PMB 01000, Gindiri, Plateau State, Nigeria.
2. Department of Plant Science & Technology, University of Jos, PMB 2084 Jos, Plateau State ,Nigeria
*Email of corresponding author: jwakfel@gmail.com
Abstract
Qualitative and quantitative postharvest losses of Sweet potato tubers occur due to pest, mechanical,
physiological and lack of appropriate storage system of the crop. The objective of this study is to seek for a safe
and cheap storage method which can preserve the tubers and retain much of the nutrients. Tubers of five sweet
potato cultivars were stored in pits treated with ash and sawdust of Shea butter(Vitellaria paradoxa) for one
hundred and fifty days (150) at the National Root Crop Research Institute (NRCRI), Kuru-Vom (latitude 09o
44’N and longitude 08o
47’E), Plateau State, Nigeria. Trease & Evans (1999) methods of phytochemical
screening were adopted for the plant materials. Proximate food analysis method by Association of Official
Analytical Chemist (AOAC, 2000) was adopted. Data collected were subjected to analysis of variance
(ANOVA) test using Genstat software version 14(2000) and the means were separated using new Duncan
Multiple Range Test. Phytochemical screening of the plant materials showed presence of Alkaloids, Resins,
Cardiac glycosides, Terpenes and Steroids. The tubers stored in sawdust appeared fresh almost as at harvest.
Proximate food nutrients show decrease but not significant in carbohydrate and nitrogen free extract (NFE) in all
the cultivars after storage. Ash treatment had more decrease in moisture than sawdust treatment and control.
Protein decreased in the cultivars but the decrease was significant (P<0.05) in CIP4400168 and TIS87/0087
cultivars. Ex-Igbariam cultivar had significant (P<0.05) increase in crude fibre. Crude ash and phosphorus
significantly (P<0.05) decreased while Lipids significantly (P<0.05) increased in all the cultivars respectively.
Calcium significantly (P<.005) increased in TIS2544Rusanya1.5, TIS86/0356 and TIS87/0087 cultivars while
CIP4400168 and Ex-Igbariam cultivars had significant (P<0.05) decrease. Tubers stored in sawdust of shea
butter gave the best preservation result. The results are further discussed.
Key words: Ash, sawdust, shea butter (Vitellaria paradoxa), sweet potato, cultivar, storage treatment.
Introduction
Sweet potato [Ipomoea batatas (L.) Lam.] production is affected by a number of factors such as
Nematodes and other animal pests in the field (Mehrota & Aggarwal, 2006; Ramesh & Tomlins, 2010; CIP,
2010), physical and physiological damages (Wikipedia, 2009; CIP, 2010), primary and secondary pathogenic
micro-organisms (Uzuegbu & Okoro, 1999; Kucharek & Robert, 2006; Bomford, 2009) and environmental
conditions. These factors predispose the crop products to various diseases and other kinds of damages leading to
qualitative and quantitative tuber postharvest losses incurred by especially the local producers of the crop. ITRA
(2006) observed that the right storage system remains a major obstacle to the production of sweet potato in many
African countries.
A safe storage of food for home use should determine the best methods that can reduce the threat of food
poisoning and risk of contamination by pathogenic microorganisms (Wikipedia, 2011). Bekele et al.(1996)
warned of the toxicity and ecological safety for the use of insecticides in storage of food products. Alternative
local storage methods such as the use of plant materials that are environmentally friendly have been found to be
effective in food storage and preservation. Enobakhare & Azeez (2006) reported the use of Neem (Azadirachta
indica) and Tobacco (Nicotiana tobacum) plant parts in controlling Bruchid (Callosobruchus maculatus) on
cowpea (Vigna unguiculata) seeds in storage. However, UNIFEM (1988) warned that the selection of plant
materials for use in the storage/preservation should be done with care. Some resinous woods, for example Pines,
may impact unpleasant flavour and taste on the final product being stored.
Plant materials are known to have phytochemicals (biochemicals) that have physiological activities on
cells or tissues of living things due to their protective and disease preventive abilities (Tiger, 1998; Tena et al.,
2001; Adamu et al., 2007). The phytochemicals inhibit enzyme and other biochemical activities of organisms
through reaction with their hydroxyl groups. They form complexes with the organism’s cells and soluble
proteins leading to adverse physiological reactions in the organism (Kubmarawa et al., 2005; Adamu et al.
2007). Godwin & Mercer (1983) have reported that cardiac glycosides contain special sugars and glycones that
have some physiological activities on organisms. Terpenes and steroids produce free radicals which react with
parasites’ proteins and nucleic acids thereby resulting in the death of the parasites (Tiger, 1998 and Moerman,
Journal of Biology, Agriculture and Healthcare www.iiste.org
ISSN 2224-3208 (Paper) ISSN 2225-093X (Online)
Vol.4, No.24, 2014
168
1998). Furthermore, Okeke & Elekwa (2006) and Adamu et al. (2007) have reported that alkaloids have toxic
effects and are important in physiological activities of organisms. The above reports on the therapeautic
activities of phytochemicals have revealed their importance in storage process and preservation of food crops.
A storage method which ensures that stored foods retain much of their nutrient quality after a reasonably
long storage period, has remained the focal point in researches aimed at curbing food losses ( Dandayo, 2010).
Similarly, Umar (2007) and Adebayo (2010) stated that good storage techniques that will make crop products
last longer and warrant the stability of food supply over time is important in reducing losses of perishable crops.
It is in the light of the above that this study seeks for an effective local storage method that can be used in
preserving sweet potato tuber surpluses during the time of harvest and makes it readily available at any time after
period of harvest with much of the food nutrients retained.
Materials and Methods
The vines of five sweet potato cultivars namely: TIS2544Rusanya1.5, CIP4400168, Ex-Igbariam,
TIS86/0356 and TIS87/0087 were collected from Plateau Agricultural Development Project (PADP)
experimental garden at Vom in Jos South Local Government Area of Plateau state, Nigeria . The cultivars were
certified by the National Root Crops Research Institute (NRCRI) Umudike in Abia State, Nigeria. The sweet
potato were cultivated for their tubers required for the storage trial at the Irish Potato Programme of the National
Root Crop Research Institute (NRCRI), Kuru-Vom station (latitude 09o
44’N and longitude 08o
47’E, 1,239.2
metre above sea level, mean annual rainfall ranges from 122mm to 149mm) in Jos South Local Government
Area of Plateau State, Nigeria.
A piece of land measuring 15 metres by 5 metres was used for the pit storage trials of the tubers. Three
pits with two replicates were dug for storage of the tubers of each cultivar, which is six (6) storage pits per
cultivar. The total number of pits used for the storage of the five cultivars was thirty (30). Thirty (30) tubers of
each cultivar were stored in each pit of three sets per cultivar with two replications, giving a total of one hundred
and eighty (180) tubers of each cultivar. The total tubers for the five cultivars stored in the pits were nine
hundred (900) tubers. The storage pits were treated with wood ash and sawdust of Shea butter (Vitellaria
paradoxa) before storing the tubers in them while the control pit had no treatment. Phytochemical screening of
the ash and sawdust of Shea butter tree (Vitellaria paradoxa) was carried out by adopting the methods of Trease
& Evans (1999).
Proximate analysis of the tuber’s food nutrients were carried out in triplicates for each cultivar at harvest
period, and later after storage of the tubers in pits treated with ash and sawdust of the Shea butter ( Vitellaria
paradoxa) for one hundred and fifty days (150DAS) respectively. The methods of proximate analysis of foods
by Association of Official Analytical Chemist (AOAC, 2000) were adopted for the analysis of the sweet potato
tuber nutrients in this study. Data collected were subjected to analysis of variance (ANOVA) test using Genstat
software version 14(2000). The new Duncan Multiple Range Test was adopted in separating the means of the
nutrients from the ANOVA test.
Results and Discussions
Phytochemical screening of the ash and sawdust of Shea butter (Vitellaria paradoxa) used in the
treatment of the pits show presence of Alkaloids, Cardiac glycosides, Terpenes and Steroids, and Resins (Table
1). The proximate food nutrients show decrease but not significant in carbohydrate and nitrogen free extract
(NFE) in all the cultivars at one hundred and fifty days after storage (150DAS). There was no significant
difference in the decrease between the treatments and also the control (Tables 2-6). There was decrease in
moisture content in all the cultivars at 150DAS (Tables 2-6) but CIP4400168, Ex-Igbariam, and TIS86/0356 had
significant (P<0.05) decrease (Tables 3, 4 and 5). Ash treatment had more decrease in moisture content than in
sawdust and control in the same cultivars. Protein decreased in all the cultivars but the decrease was significant
(P<0.05) in CIP4400168 and TIS87/0087 (Tables 3 and 6). The decrease was less in sawdust treatment than in
ash. The crude fibre decreased in TIS2544Rusanya1.5, CIP4400168 and TIS87/0087 but further decreased
significantly (P<0.05) in TIS86/0356. However, Ex-Igbariam had significant (P<0.05) increase in crude fibre at
150DAS (Tables 2-6). Lipid had a significant (P<0.05) increase but crude ash and phosphorus significantly
(P<0.05) decreased in all the cultivars respectively at 150DAS (Tables 2-6). Calcium had significant (P<0.05)
increase in cultivars TIS2544Rusanya1.5, TIS86/0356 and TIS87/0087 while CIP4400168 and Ex-Igbariam
significantly (P<0.05) decreased. Ash treatment had more calcium but not significantly than sawdust treatments
in the cultivars that had increase at 150DAS.
Tuber as a living organ is capable of undergoing biochemical syntheses while in storage leading to
possible increase or decrease in some nutrients as was observed in the five cultivars of sweet potato in this study.
Increase or decrease in the content of plant materials and their nutrient values is also attributed to the activities of
the microorganisms and storage environment (Mehrotra & Aggarwal, 2006, Ugoh & Akueshi, 2008).
Journal of Biology, Agriculture and Healthcare www.iiste.org
ISSN 2224-3208 (Paper) ISSN 2225-093X (Online)
Vol.4, No.24, 2014
169
Storage conditions (temperature, moisture, light, environment, cultivar, plant age/maturity, geographic or
climatic effects and agricultural practices/handling process) can affect plant nutrients positively or negatively
during storage (Idah et al., 2010; Lawal, 2012). For example, freezing (temperature) can cause breakage of cell
membranes leading to discharge of cell content including mineral elements. Freezing can also alter plant pigment
leading to alteration in the nutrient synthesis by the plant. Johnson (1988) reported that some nutrients decrease
or increase with increase in age of plant materials. The decrease or increase observed in the food proximate
nutrients of the tubers of the five cultivars of the sweet potato after storage for 150days in this study is therefore;
in line with the above reports.
Furthermore, Lawal (2012) reported decrease in nutrient contents of melon seeds(Citrullus lanatus) with
increase time of storage. Similarly, Musa and Ogbadoyi(2014) reported reduction in the nutrient, anti-nutrient
and toxic content level in bitter leaf (Vernonia amygdalina) after two weeks of cold storage condition in freezer
at -4o
C. Onifade et al.(2003) reported significant decrease in potassium(K), magnesium(Mg) and
calcium(Ca), but an increase in zinc(Zn), iron(Fe) and sodium( Na) mineral elements in sweet potato
after storage. Suraji et al.(2013) reported low level of calcium in five orange fleshed cultivars of sweet potato.
Idah et al.(2010) reported decrease in the nutrients of tomato and orange after periods of storage. However, Idah
et al.(2010) were quick to note that storage that prevents light can keep the nutrients intact. They further reported
that some nutrients such as protein, carbohydrates, etc have polar or charge side chains, these chains can react
with other molecules in different ways leading to increase or decrease in some of the nutrients.
It has also been reported that environment, location, climatic conditions, cultivar, etc could affect the
distribution of nutrients, polyphenolic and antioxidants in sweet potato (Choong et al., 2007; Hamouz et al.,
2011). Similarly, Eleazu & Ironua (2013) opined that environment and climatic conditions could affect the
distribution of the food parameters (nutrients) in sweet potato. However, Bosede & Musa (2010) reported an
increase of between 13.54% to 46.39% in the dietary fibre of peanut butter after storage period of three months.
In the same vane, Fagbohun & Lawal(2011) reported increase in ash and crude protein content in sundried
soybean stored for twenty weeks. The reported decrease or increase in some of the nutrients depending on the
age of the plant materials is a confirmation of what was observed in this study.
Conclusion
The storage of sweet potato tubers in sawdust of shea butter (Vitellaria sparadoxa ) gave the best results in
term of preserving the shelf life of the tubers. The presence of some of the phytochemicals analysed from the ash
and sawdust of shea butter (Vitellaria paradoxa) is suggestive of their therapeutic activities in determining the
health status of the tubers after the storage period in this study. The increase in lipid content in all the cultivars
between at harvest period and after storage is important information about sweet potato lipid. Cultivars
TIS2544Rusanya1.5 and Ex-Igbariam had significant (P<0.05) increase in Calcium and crude fibre which are
required in man’s diet. The control had consistent more decrease in the nutrients than in the treatments. The
decrease in the nutrients which was less in the treatments as compared to the control after storage is a
contribution to knowledge as to the desirability for a storage method that can preserve and retain much of the
food nutrients of the tubers after a relatively long storage period. The use of ash and sawdust of Shea butter
(Vitellaria paradoxa) plant materials is recommended as a possible local storage method in preserving sweet
potato tubers.
Acknowledgement
The authors wish to appreciate the Co-coordinator National Root Crop Research Institute (NRCRI),
Kuru-Vom for providing us with piece of land in the Institute to carry out the experiment and staff of the
Institute for their assistance. The Director and staff of the Department of Biochemistry National Veterinary
Research Institute, Vom, the Head of Plant Science & Technology and laboratory staff, University of Jos,
Plateau State, Nigeria are equally acknowledged for their technical assistance.
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mw-head
Table 1: Phytochemical Determination of Ash and Sawdust
Phytochemicals Sawdust Ash
Alkaloids + +
Flavonoids - -
Tannins - -
Saponins - -
Balsam - -
Cardiac glycosides + +
Terpenes and
Steriods
+ +
Resins + +
Phenols - -
Key:
+ = Present or detected
- = absent or not detected
Table 2. Effect of Storage Treatments on the Nutritional Contents of TIS2544 Rusanya 1.5 Cultivar of Sweet Potato
Storage treatment Carbohydrate
(kcal.)
Moisture
(g)
Crude protein
(g)
Crude
fibre
(g)
Lipids
(g)
Total ash
(g)
NFE
(mg)
Calcium
(mg)
Phosphoru
s
(mg)
Harvest 382.00 a
* 5.25 a *
3.39a*
2.05 a*
1.00b
3.70 a
89.60a*
0.45b
0.96a
Ash 339.82 a
5.16 a
3.28 a
1.90 a
2.45a * 2.11b*
72.85 a
0.75a*
0.10b *
Sawdust 341.73 a
5.14 a
3.76 a
1.68 a
2.32a
2.18b
72.67 a
0.82a
0.08b
Control
S E ±
332.58
55.41
5.12
1.76
3.17 a
0.89
2.04 a
0.47
2.44a
0.62
2.07b
0.97
72.81 a
16.21
0.77a
0.21
0.09b
0.06
* Means followed by the same letter(s) do not differ significantly at 5% level probability (Duncan’s new
Multiple Range Test = DMRT).
Journal of Biology, Agriculture and Healthcare www.iiste.org
ISSN 2224-3208 (Paper) ISSN 2225-093X (Online)
Vol.4, No.24, 2014
172
Table 3: Effect of Storage Treatments on the Nutritional Contents of CIP4400168 Cultivar of
Sweet Potato.
Storage
Treatment
Carbohydrate
(kcal.)
Moisture
(g)
Crude
Protein
(g)
Crude
Fibre
(g)
Lipids
(g)
Total
Ash
(g)
NFE
(mg)
Calcium
(mg)
Phosphorus
(mg)
Harvest
Ash
388.00 a*
374.68 a
5.70a
4.23d
3.54a
2.53b*
2.70 a*
2.55 a
1.71b
2.92a*
3.33a
1.43b*
90.65 a*
83.57 a
0.42a
0.21b*
0.14a
0.10b
Sawdust 374.53 a
5.32bc
2.49b
2.60 a
2.89a
1.38b
84.12 a
0.19b
0.12ab
Control 366.70 a
5.58ab
2.25b
1.33 a
2.83a
1.29b
79.94 a
0.20b
0.11b
S E ± 51.23 1.32 0.61 0.39 0.45 0.64 17.1 0.07 0.02
* Means followed by the same letter(s) do not differ significantly at 5% level of probability (Duncan’s new
Multiple Range Test =DMRT)
Table 4: Effect of Storage Treatments on the Nutritional Contents of Ex- Igbariam cultivar of Sweet potato
Storage
Treatment
Carbohydrate
(kcal.)
Moisture
(g)
Crude
Protein
(g)
Crude
fibre
(g)
Lipids
(g)
Total
Ash
(g)
NFE
(mg)
Calcium
(mg)
Phosphorus
(mg)
Harvest
Ash
361.00a*
364.88 a
7.78a
5.13b*
3.29 a*
2.59 a
5.83b
10.59a*
1.44b
2.68a*
5.69a
1.54b*
83.75 a*
82.44 a
0.44a
0.27b*
0.15a
0.10b*
Sawdust 359.72 a
5.59b
2.87 a
10.23a
2.73a
1.67b
84.31 a
0.25b
0.11b
Control
SE±
363.94 a
49.21
5.20b
1.60
2.73 a
1.04
10.53a
2.56
2.63a
0.34
1.57b
0.87
83.76 a
17.80
0.26b
0.09
0.10b
0.02
* Means followed by the same letter(s) do not differ significantly at 5% level of probability (Duncan’s new
Multiple Range Test =DMRT)
Table 5: Effect of Storage Treatments on the Nutritional Contents of TIS86/0356 cultivar of Sweet potato.
Storage
Treatment
Carbohydrate
(kcal.)
Moisture
(g)
Crude
Protein
(g)
Crude
fibre
(g)
Lipids
(g)
Total
Ash
(g)
NFE
(mg)
Calcium
(mg)
Phosphorus
(mg)
Harvest
Ash
369.00 a*
359.81 a
5.96a
5.20b*
3.15 a*
3.34 a
5.68a
4.23b*
1.10b
2.93a*
5.80a
1.48b
84.25 a*
79.02 a
0.38b
0.64a*
0.18a
0.11b*
Sawdust 358.43 a
5.53ab
2.97 a
4.14b
2.96a
1.47b
79.12 a
0.66a
0.09b
Control
SE±
358.61 a
52.76
5.25ab
0.75
3.31 a
0.74
4.32b
1.12
2.91a
0.33
1.46b
0.57
78.93 a
16.87
0.63a
0.25
0.10b
0.05
* Means followed by the same letter(s) do not differ significantly at 5% level of probability (Duncan’s new
Multiple Range Test = DMRT)
Table 6: Effect of Storage Treatments on the Nutritional Contents of TIS87/0087 cultivar of Sweet potato.
Storage
Treatment
Carbohydrate
(kcal.)
Moisture
(g)
Crude
Protein
(g)
Crude
Fibre
(g)
Lipids
(g)
Lipids ash
(g)
NFE
(mg)
Calcium
(mg)
Phosphorus
(mg)
Harvest
Ash
352.50 a*
341.49 a
6.84 a*
6.41 a
3.85a
2.97b*
5.65 a*
5.60 a
0.50b
2.73a*
6.85a
2.29b*
83.15 a*
76.26 a
0.51b
0.64a*
0.19a*
0.11b
Sawdust 342.43 a
6.43 a
3.25ab
5.26 a
2.75a
2.13b
75.82 a
0.58a
0.14a
Control
SE±
342.11 a
52.12
6.40 a
1.54
3.03b
0.45
5.40 a
0.41
2.72a
0.28
2.22b
0.89
75.87 a
15.74
0.63a
0.08
0.11b
0.07
* Means followed by the same letter(s) do not differ significantly at 5% level of probability (Duncan’s new
Multiple Range Test = DMRT
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Effect of ash and sawdust of shear butter

  • 1. Journal of Biology, Agriculture and Healthcare www.iiste.org ISSN 2224-3208 (Paper) ISSN 2225-093X (Online) Vol.4, No.24, 2014 167 Effect of Ash and Sawdust of Shear Butter) (Vitellaria paradoxa ) Storage Treatments on The Tuber Nutrient of Some Sweet Potato [Ipomoea batatas (L.) Lam.] Cultivars F.S Jwakdak 1* & C.O Akueshi 2 1. Department of Biology, College of Education, PMB 01000, Gindiri, Plateau State, Nigeria. 2. Department of Plant Science & Technology, University of Jos, PMB 2084 Jos, Plateau State ,Nigeria *Email of corresponding author: jwakfel@gmail.com Abstract Qualitative and quantitative postharvest losses of Sweet potato tubers occur due to pest, mechanical, physiological and lack of appropriate storage system of the crop. The objective of this study is to seek for a safe and cheap storage method which can preserve the tubers and retain much of the nutrients. Tubers of five sweet potato cultivars were stored in pits treated with ash and sawdust of Shea butter(Vitellaria paradoxa) for one hundred and fifty days (150) at the National Root Crop Research Institute (NRCRI), Kuru-Vom (latitude 09o 44’N and longitude 08o 47’E), Plateau State, Nigeria. Trease & Evans (1999) methods of phytochemical screening were adopted for the plant materials. Proximate food analysis method by Association of Official Analytical Chemist (AOAC, 2000) was adopted. Data collected were subjected to analysis of variance (ANOVA) test using Genstat software version 14(2000) and the means were separated using new Duncan Multiple Range Test. Phytochemical screening of the plant materials showed presence of Alkaloids, Resins, Cardiac glycosides, Terpenes and Steroids. The tubers stored in sawdust appeared fresh almost as at harvest. Proximate food nutrients show decrease but not significant in carbohydrate and nitrogen free extract (NFE) in all the cultivars after storage. Ash treatment had more decrease in moisture than sawdust treatment and control. Protein decreased in the cultivars but the decrease was significant (P<0.05) in CIP4400168 and TIS87/0087 cultivars. Ex-Igbariam cultivar had significant (P<0.05) increase in crude fibre. Crude ash and phosphorus significantly (P<0.05) decreased while Lipids significantly (P<0.05) increased in all the cultivars respectively. Calcium significantly (P<.005) increased in TIS2544Rusanya1.5, TIS86/0356 and TIS87/0087 cultivars while CIP4400168 and Ex-Igbariam cultivars had significant (P<0.05) decrease. Tubers stored in sawdust of shea butter gave the best preservation result. The results are further discussed. Key words: Ash, sawdust, shea butter (Vitellaria paradoxa), sweet potato, cultivar, storage treatment. Introduction Sweet potato [Ipomoea batatas (L.) Lam.] production is affected by a number of factors such as Nematodes and other animal pests in the field (Mehrota & Aggarwal, 2006; Ramesh & Tomlins, 2010; CIP, 2010), physical and physiological damages (Wikipedia, 2009; CIP, 2010), primary and secondary pathogenic micro-organisms (Uzuegbu & Okoro, 1999; Kucharek & Robert, 2006; Bomford, 2009) and environmental conditions. These factors predispose the crop products to various diseases and other kinds of damages leading to qualitative and quantitative tuber postharvest losses incurred by especially the local producers of the crop. ITRA (2006) observed that the right storage system remains a major obstacle to the production of sweet potato in many African countries. A safe storage of food for home use should determine the best methods that can reduce the threat of food poisoning and risk of contamination by pathogenic microorganisms (Wikipedia, 2011). Bekele et al.(1996) warned of the toxicity and ecological safety for the use of insecticides in storage of food products. Alternative local storage methods such as the use of plant materials that are environmentally friendly have been found to be effective in food storage and preservation. Enobakhare & Azeez (2006) reported the use of Neem (Azadirachta indica) and Tobacco (Nicotiana tobacum) plant parts in controlling Bruchid (Callosobruchus maculatus) on cowpea (Vigna unguiculata) seeds in storage. However, UNIFEM (1988) warned that the selection of plant materials for use in the storage/preservation should be done with care. Some resinous woods, for example Pines, may impact unpleasant flavour and taste on the final product being stored. Plant materials are known to have phytochemicals (biochemicals) that have physiological activities on cells or tissues of living things due to their protective and disease preventive abilities (Tiger, 1998; Tena et al., 2001; Adamu et al., 2007). The phytochemicals inhibit enzyme and other biochemical activities of organisms through reaction with their hydroxyl groups. They form complexes with the organism’s cells and soluble proteins leading to adverse physiological reactions in the organism (Kubmarawa et al., 2005; Adamu et al. 2007). Godwin & Mercer (1983) have reported that cardiac glycosides contain special sugars and glycones that have some physiological activities on organisms. Terpenes and steroids produce free radicals which react with parasites’ proteins and nucleic acids thereby resulting in the death of the parasites (Tiger, 1998 and Moerman,
  • 2. Journal of Biology, Agriculture and Healthcare www.iiste.org ISSN 2224-3208 (Paper) ISSN 2225-093X (Online) Vol.4, No.24, 2014 168 1998). Furthermore, Okeke & Elekwa (2006) and Adamu et al. (2007) have reported that alkaloids have toxic effects and are important in physiological activities of organisms. The above reports on the therapeautic activities of phytochemicals have revealed their importance in storage process and preservation of food crops. A storage method which ensures that stored foods retain much of their nutrient quality after a reasonably long storage period, has remained the focal point in researches aimed at curbing food losses ( Dandayo, 2010). Similarly, Umar (2007) and Adebayo (2010) stated that good storage techniques that will make crop products last longer and warrant the stability of food supply over time is important in reducing losses of perishable crops. It is in the light of the above that this study seeks for an effective local storage method that can be used in preserving sweet potato tuber surpluses during the time of harvest and makes it readily available at any time after period of harvest with much of the food nutrients retained. Materials and Methods The vines of five sweet potato cultivars namely: TIS2544Rusanya1.5, CIP4400168, Ex-Igbariam, TIS86/0356 and TIS87/0087 were collected from Plateau Agricultural Development Project (PADP) experimental garden at Vom in Jos South Local Government Area of Plateau state, Nigeria . The cultivars were certified by the National Root Crops Research Institute (NRCRI) Umudike in Abia State, Nigeria. The sweet potato were cultivated for their tubers required for the storage trial at the Irish Potato Programme of the National Root Crop Research Institute (NRCRI), Kuru-Vom station (latitude 09o 44’N and longitude 08o 47’E, 1,239.2 metre above sea level, mean annual rainfall ranges from 122mm to 149mm) in Jos South Local Government Area of Plateau State, Nigeria. A piece of land measuring 15 metres by 5 metres was used for the pit storage trials of the tubers. Three pits with two replicates were dug for storage of the tubers of each cultivar, which is six (6) storage pits per cultivar. The total number of pits used for the storage of the five cultivars was thirty (30). Thirty (30) tubers of each cultivar were stored in each pit of three sets per cultivar with two replications, giving a total of one hundred and eighty (180) tubers of each cultivar. The total tubers for the five cultivars stored in the pits were nine hundred (900) tubers. The storage pits were treated with wood ash and sawdust of Shea butter (Vitellaria paradoxa) before storing the tubers in them while the control pit had no treatment. Phytochemical screening of the ash and sawdust of Shea butter tree (Vitellaria paradoxa) was carried out by adopting the methods of Trease & Evans (1999). Proximate analysis of the tuber’s food nutrients were carried out in triplicates for each cultivar at harvest period, and later after storage of the tubers in pits treated with ash and sawdust of the Shea butter ( Vitellaria paradoxa) for one hundred and fifty days (150DAS) respectively. The methods of proximate analysis of foods by Association of Official Analytical Chemist (AOAC, 2000) were adopted for the analysis of the sweet potato tuber nutrients in this study. Data collected were subjected to analysis of variance (ANOVA) test using Genstat software version 14(2000). The new Duncan Multiple Range Test was adopted in separating the means of the nutrients from the ANOVA test. Results and Discussions Phytochemical screening of the ash and sawdust of Shea butter (Vitellaria paradoxa) used in the treatment of the pits show presence of Alkaloids, Cardiac glycosides, Terpenes and Steroids, and Resins (Table 1). The proximate food nutrients show decrease but not significant in carbohydrate and nitrogen free extract (NFE) in all the cultivars at one hundred and fifty days after storage (150DAS). There was no significant difference in the decrease between the treatments and also the control (Tables 2-6). There was decrease in moisture content in all the cultivars at 150DAS (Tables 2-6) but CIP4400168, Ex-Igbariam, and TIS86/0356 had significant (P<0.05) decrease (Tables 3, 4 and 5). Ash treatment had more decrease in moisture content than in sawdust and control in the same cultivars. Protein decreased in all the cultivars but the decrease was significant (P<0.05) in CIP4400168 and TIS87/0087 (Tables 3 and 6). The decrease was less in sawdust treatment than in ash. The crude fibre decreased in TIS2544Rusanya1.5, CIP4400168 and TIS87/0087 but further decreased significantly (P<0.05) in TIS86/0356. However, Ex-Igbariam had significant (P<0.05) increase in crude fibre at 150DAS (Tables 2-6). Lipid had a significant (P<0.05) increase but crude ash and phosphorus significantly (P<0.05) decreased in all the cultivars respectively at 150DAS (Tables 2-6). Calcium had significant (P<0.05) increase in cultivars TIS2544Rusanya1.5, TIS86/0356 and TIS87/0087 while CIP4400168 and Ex-Igbariam significantly (P<0.05) decreased. Ash treatment had more calcium but not significantly than sawdust treatments in the cultivars that had increase at 150DAS. Tuber as a living organ is capable of undergoing biochemical syntheses while in storage leading to possible increase or decrease in some nutrients as was observed in the five cultivars of sweet potato in this study. Increase or decrease in the content of plant materials and their nutrient values is also attributed to the activities of the microorganisms and storage environment (Mehrotra & Aggarwal, 2006, Ugoh & Akueshi, 2008).
  • 3. Journal of Biology, Agriculture and Healthcare www.iiste.org ISSN 2224-3208 (Paper) ISSN 2225-093X (Online) Vol.4, No.24, 2014 169 Storage conditions (temperature, moisture, light, environment, cultivar, plant age/maturity, geographic or climatic effects and agricultural practices/handling process) can affect plant nutrients positively or negatively during storage (Idah et al., 2010; Lawal, 2012). For example, freezing (temperature) can cause breakage of cell membranes leading to discharge of cell content including mineral elements. Freezing can also alter plant pigment leading to alteration in the nutrient synthesis by the plant. Johnson (1988) reported that some nutrients decrease or increase with increase in age of plant materials. The decrease or increase observed in the food proximate nutrients of the tubers of the five cultivars of the sweet potato after storage for 150days in this study is therefore; in line with the above reports. Furthermore, Lawal (2012) reported decrease in nutrient contents of melon seeds(Citrullus lanatus) with increase time of storage. Similarly, Musa and Ogbadoyi(2014) reported reduction in the nutrient, anti-nutrient and toxic content level in bitter leaf (Vernonia amygdalina) after two weeks of cold storage condition in freezer at -4o C. Onifade et al.(2003) reported significant decrease in potassium(K), magnesium(Mg) and calcium(Ca), but an increase in zinc(Zn), iron(Fe) and sodium( Na) mineral elements in sweet potato after storage. Suraji et al.(2013) reported low level of calcium in five orange fleshed cultivars of sweet potato. Idah et al.(2010) reported decrease in the nutrients of tomato and orange after periods of storage. However, Idah et al.(2010) were quick to note that storage that prevents light can keep the nutrients intact. They further reported that some nutrients such as protein, carbohydrates, etc have polar or charge side chains, these chains can react with other molecules in different ways leading to increase or decrease in some of the nutrients. It has also been reported that environment, location, climatic conditions, cultivar, etc could affect the distribution of nutrients, polyphenolic and antioxidants in sweet potato (Choong et al., 2007; Hamouz et al., 2011). Similarly, Eleazu & Ironua (2013) opined that environment and climatic conditions could affect the distribution of the food parameters (nutrients) in sweet potato. However, Bosede & Musa (2010) reported an increase of between 13.54% to 46.39% in the dietary fibre of peanut butter after storage period of three months. In the same vane, Fagbohun & Lawal(2011) reported increase in ash and crude protein content in sundried soybean stored for twenty weeks. The reported decrease or increase in some of the nutrients depending on the age of the plant materials is a confirmation of what was observed in this study. Conclusion The storage of sweet potato tubers in sawdust of shea butter (Vitellaria sparadoxa ) gave the best results in term of preserving the shelf life of the tubers. The presence of some of the phytochemicals analysed from the ash and sawdust of shea butter (Vitellaria paradoxa) is suggestive of their therapeutic activities in determining the health status of the tubers after the storage period in this study. The increase in lipid content in all the cultivars between at harvest period and after storage is important information about sweet potato lipid. Cultivars TIS2544Rusanya1.5 and Ex-Igbariam had significant (P<0.05) increase in Calcium and crude fibre which are required in man’s diet. The control had consistent more decrease in the nutrients than in the treatments. The decrease in the nutrients which was less in the treatments as compared to the control after storage is a contribution to knowledge as to the desirability for a storage method that can preserve and retain much of the food nutrients of the tubers after a relatively long storage period. The use of ash and sawdust of Shea butter (Vitellaria paradoxa) plant materials is recommended as a possible local storage method in preserving sweet potato tubers. Acknowledgement The authors wish to appreciate the Co-coordinator National Root Crop Research Institute (NRCRI), Kuru-Vom for providing us with piece of land in the Institute to carry out the experiment and staff of the Institute for their assistance. The Director and staff of the Department of Biochemistry National Veterinary Research Institute, Vom, the Head of Plant Science & Technology and laboratory staff, University of Jos, Plateau State, Nigeria are equally acknowledged for their technical assistance. References Adamu, H. 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  • 4. Journal of Biology, Agriculture and Healthcare www.iiste.org ISSN 2224-3208 (Paper) ISSN 2225-093X (Online) Vol.4, No.24, 2014 170 wordpress.com/2009/04/14/ Bosede, O. & Musa, J. J. (2010). Effect of Storage on the Nutritional Qualities of Peanut butter. International Journal of Agricultural Research, 7(1), 112-125. Choong, C. T.,Van-Dan, T. A., Roger, A., McFeeters, F., Roger, L., Thompson, K. V., Pecota, G., Craig, Y. (2007). Antioxidant activities, phenolic and beta-carotene content of sweet potato genotype with varying flesh colours. Food chemistry, 103 : 829-838 Dandayo, M. A. (2010). Post harvest Handling and Transportation of Oranges in Yola, Nigeria. International Journal of Food and Agricultural Research, 7(1), 36-43. Egan, H., Kirk., R & Sawyer, R. (1988). Pearson’s Chemical Analysis of Foods. 8th Edition. England U.K: Longman Scientific & Technical Group Ltd. Eleazu, C.O. & Ironua, C. (2013). Physicochemical composition and Antioxidant properties of Sweet potato variety (Ipomoea batatas L.) commercially sold in South Eastern Nigeria. African Journal of Biotechnology, 12(17): 720-727. Enobakhare, D. A. & Azeez, O. M. (2006). Comparative Efficacy of Neem( Azadirachta indica) and Tobacco(Nicotiana tobacum) Plant Parts in Controlling Cowpea(Vigna unguiculata) Seed Storage Bruchid(Callosobruchus maculates Fabricius) , Journal of Agricultural Forestry and Fisheries, 7(1), 25- 32. Fagbohun, E. D. & Lawal, O. U. (2011). Effect of storage on nutrient composition and mycobiota of sundried soybean. African Journal of Food Sciences, 5(8): 473-477. Genstat(2000). Genstat Release Version 14(PC) window vistal). VSN International Ltd Godwin, T. & Mercer, E. T. (1983). Introduction to Plant Biochemistry, 2nd Edition, New York NY: Perganmon Press. Hamouz, K., Lachman, J., Pazderu, K., Tomasek, J., Hejtmankova, K. & Pivec, V. (2011). Differences in anthocyanin content and antioxidant activity of potato tubers with different flesh colours . Plant Soil Environment, 57(10): 478-485 Idah, P.A., Musa, J.J. & Abdullahi, M. (2010). Effects of Storage Period on Some Nutritional Properties of Orange and Tomato. AU Journal of Technology. 13(3): 181-185 Institut Togolaise de Recherche Agronomique (ITRA)( 2006 ). La patate douce au Togo. pp.12 International Potato Centre (CIP). (2010). CIP newsletter. Retrieved Aug 10th 2010, 4.32 pm from CIP webmaster@cgiar.org Johnson, C. (1988). Effect of plant age on element concentration in parts of Desmodium introvum CV green leaf. Common Soil Science and Plant Analysis, 279 – 298. Kubmarawa, D., George, A., Kidah, M. I. & Gabdo, M. B. (2005). Studies on Phytochemical and Antimicrobial Evaluation of Extracts of Boswellia dalzielli( Hutch). Nigeria Journal of Biotechnology, 16(1), 83-90. Kucharek, T. A. & Robert, T. A. (2006). Sweet Potato specific common disease: Dept. of plant Pathology, 2006 Florida plant disease management guide. Florida co-operator Extension service. Institute of food and Agric. Science University of Florida. (http:// edis.Ifas. u=UFL. Edu. (Accessed on 21/2/2006, 12.25 pm). Lawal, O. U. (2012). Effect of storage on nutrient composition and the mycobiota of sundried water melon seeds (Citullus lanatus). Journal of Microbiology, Biotechnology and Food Science, 1(3): 267- 276 Mehrotra, R. S. & Aggarwal, A. (2006). Plant Pathology, 2nd edn. New Delhi: Tata Mc Graw-Hill Publising Company Ltd. Moerman, D. E. (1998). Native American Ethnobotany. Orego Timber Press Ltd, 473-475. Musa, A. & Ogbadoyi, N. (2014). Effect of cold storage on the concentration of some nutrients, Antinutrients and Toxic substances in the leaves of bitter leaf ( Venonia amygdalina). Advances in Research, 2(1): 24- 29 Okeke, C. U. & Elekwa, I. (2006). Proximate and Preliminary Phytochemical Analysis of Avocado Pear (Persea gratissima Gaertn. F.(Family Lauraceae). Nigerian Journal of Botany, 19(1), 156-162 Onifade, A., H., Atum, .N. & Adebolu, T.T. (2004).Nutrition Enrichment of Sweet Potato (Ipomoea batatas L) by Solid Substrate fermentation using Four Fungal species. Global Journal of Pure Applied Science, 10(60):31-36. Ramesh, R. & Tomlins, K. I. (2010). Sweet Potato: Postharvest Aspects in Food, Feed and Industry. Retrieved August 11th , 2010, 2.36 am from https://www.novapublishers.com/catalog/images/9781608763436.jpg Suraji, A.S., Ravaweera, A.G. & Arthur, B.(2013). Comparative analysis of nutritional quality of five different cultivars of sweet potato[Ipomea batatas (L.)] in Sri Lanka. Food Science and Nutrition. Published by Wiley Periodicals. Inc. DOI:10.1002/fsn 3.38 OR htt://onlinelibrary.wiley.com/jossurnal/10.1002(ISSN)2040.7177/earlyview Tena, L., Messia, K., Ngimbi, N. P., Chimwami, B., Okond’ahalla, C. K., DeBruyne, T……Vlietinck, A. J. (2001). In-vivo Antimalarial Activity of Cassia occidentalis, Morinda morinda and Phyllanthus niruri.
  • 5. Journal of Biology, Agriculture and Healthcare www.iiste.org ISSN 2224-3208 (Paper) ISSN 2225-093X (Online) Vol.4, No.24, 2014 171 Annals of Medicine and Parasitology 95(1), 47- 57. Tiger, L. (1998). The Nature guide to the Medicinal Herbs and plants, 1st edn, Twitchenhanze (UK): Tiger Books Plc. Trease, G. E. & Evans, W. C. (1999). Pharmacognosy, Nottingham U.K: W.B Saunders Company Ltd, 310 - 315. Ugoh, S.C. & Akueshi, C.O. (2008). Nutritional Qualities of Infected Seeds of Ogbono(Irvingia gabonensis. Aubry. Leconte Ex. ‘O’ Rorke(Bail). Nigerian Journal of Botany. 21(1) :195-201 Umar, A. B. (2007). Postharvest Losses on Tomato, Pepper and Lettuce: A case of Ajiwa Irrigation Scheme of Katsina State, Nigeria. International Journal of Food and Agricultural Research, 4(1 &2), 216 - 221 UNIFEM. (1988). Fish Processing: United Nations Development Fund for Women (UNIFEM) 4, Food Cycle Technology Source Book. Food Processing (1988). 85. Uzuegbu, J. O. & Okoro, O. K. (1999). Protection of mechanically injured yellow yam(Dioscorea cayensis) tubers from fungal rot using pastes of some local herbs. Journal of Sustain Agricultural Environment 1(2), 26 - 266. Wikipedia. (2009). 2008 World Food Prices Crisis. Retrieved 12/7/2010. 8.32 pm from http://en.wikipedia.org/wiki/2007a- Wikipedia. (2011). Food Storage. Retrieved 11/3/2012. 4.32 am from http://en.wikipedia.org/wiki/foodstorage- mw-head Table 1: Phytochemical Determination of Ash and Sawdust Phytochemicals Sawdust Ash Alkaloids + + Flavonoids - - Tannins - - Saponins - - Balsam - - Cardiac glycosides + + Terpenes and Steriods + + Resins + + Phenols - - Key: + = Present or detected - = absent or not detected Table 2. Effect of Storage Treatments on the Nutritional Contents of TIS2544 Rusanya 1.5 Cultivar of Sweet Potato Storage treatment Carbohydrate (kcal.) Moisture (g) Crude protein (g) Crude fibre (g) Lipids (g) Total ash (g) NFE (mg) Calcium (mg) Phosphoru s (mg) Harvest 382.00 a * 5.25 a * 3.39a* 2.05 a* 1.00b 3.70 a 89.60a* 0.45b 0.96a Ash 339.82 a 5.16 a 3.28 a 1.90 a 2.45a * 2.11b* 72.85 a 0.75a* 0.10b * Sawdust 341.73 a 5.14 a 3.76 a 1.68 a 2.32a 2.18b 72.67 a 0.82a 0.08b Control S E ± 332.58 55.41 5.12 1.76 3.17 a 0.89 2.04 a 0.47 2.44a 0.62 2.07b 0.97 72.81 a 16.21 0.77a 0.21 0.09b 0.06 * Means followed by the same letter(s) do not differ significantly at 5% level probability (Duncan’s new Multiple Range Test = DMRT).
  • 6. Journal of Biology, Agriculture and Healthcare www.iiste.org ISSN 2224-3208 (Paper) ISSN 2225-093X (Online) Vol.4, No.24, 2014 172 Table 3: Effect of Storage Treatments on the Nutritional Contents of CIP4400168 Cultivar of Sweet Potato. Storage Treatment Carbohydrate (kcal.) Moisture (g) Crude Protein (g) Crude Fibre (g) Lipids (g) Total Ash (g) NFE (mg) Calcium (mg) Phosphorus (mg) Harvest Ash 388.00 a* 374.68 a 5.70a 4.23d 3.54a 2.53b* 2.70 a* 2.55 a 1.71b 2.92a* 3.33a 1.43b* 90.65 a* 83.57 a 0.42a 0.21b* 0.14a 0.10b Sawdust 374.53 a 5.32bc 2.49b 2.60 a 2.89a 1.38b 84.12 a 0.19b 0.12ab Control 366.70 a 5.58ab 2.25b 1.33 a 2.83a 1.29b 79.94 a 0.20b 0.11b S E ± 51.23 1.32 0.61 0.39 0.45 0.64 17.1 0.07 0.02 * Means followed by the same letter(s) do not differ significantly at 5% level of probability (Duncan’s new Multiple Range Test =DMRT) Table 4: Effect of Storage Treatments on the Nutritional Contents of Ex- Igbariam cultivar of Sweet potato Storage Treatment Carbohydrate (kcal.) Moisture (g) Crude Protein (g) Crude fibre (g) Lipids (g) Total Ash (g) NFE (mg) Calcium (mg) Phosphorus (mg) Harvest Ash 361.00a* 364.88 a 7.78a 5.13b* 3.29 a* 2.59 a 5.83b 10.59a* 1.44b 2.68a* 5.69a 1.54b* 83.75 a* 82.44 a 0.44a 0.27b* 0.15a 0.10b* Sawdust 359.72 a 5.59b 2.87 a 10.23a 2.73a 1.67b 84.31 a 0.25b 0.11b Control SE± 363.94 a 49.21 5.20b 1.60 2.73 a 1.04 10.53a 2.56 2.63a 0.34 1.57b 0.87 83.76 a 17.80 0.26b 0.09 0.10b 0.02 * Means followed by the same letter(s) do not differ significantly at 5% level of probability (Duncan’s new Multiple Range Test =DMRT) Table 5: Effect of Storage Treatments on the Nutritional Contents of TIS86/0356 cultivar of Sweet potato. Storage Treatment Carbohydrate (kcal.) Moisture (g) Crude Protein (g) Crude fibre (g) Lipids (g) Total Ash (g) NFE (mg) Calcium (mg) Phosphorus (mg) Harvest Ash 369.00 a* 359.81 a 5.96a 5.20b* 3.15 a* 3.34 a 5.68a 4.23b* 1.10b 2.93a* 5.80a 1.48b 84.25 a* 79.02 a 0.38b 0.64a* 0.18a 0.11b* Sawdust 358.43 a 5.53ab 2.97 a 4.14b 2.96a 1.47b 79.12 a 0.66a 0.09b Control SE± 358.61 a 52.76 5.25ab 0.75 3.31 a 0.74 4.32b 1.12 2.91a 0.33 1.46b 0.57 78.93 a 16.87 0.63a 0.25 0.10b 0.05 * Means followed by the same letter(s) do not differ significantly at 5% level of probability (Duncan’s new Multiple Range Test = DMRT) Table 6: Effect of Storage Treatments on the Nutritional Contents of TIS87/0087 cultivar of Sweet potato. Storage Treatment Carbohydrate (kcal.) Moisture (g) Crude Protein (g) Crude Fibre (g) Lipids (g) Lipids ash (g) NFE (mg) Calcium (mg) Phosphorus (mg) Harvest Ash 352.50 a* 341.49 a 6.84 a* 6.41 a 3.85a 2.97b* 5.65 a* 5.60 a 0.50b 2.73a* 6.85a 2.29b* 83.15 a* 76.26 a 0.51b 0.64a* 0.19a* 0.11b Sawdust 342.43 a 6.43 a 3.25ab 5.26 a 2.75a 2.13b 75.82 a 0.58a 0.14a Control SE± 342.11 a 52.12 6.40 a 1.54 3.03b 0.45 5.40 a 0.41 2.72a 0.28 2.22b 0.89 75.87 a 15.74 0.63a 0.08 0.11b 0.07 * Means followed by the same letter(s) do not differ significantly at 5% level of probability (Duncan’s new Multiple Range Test = DMRT
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