EasyGene oligo factory

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Oligo Factory

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EasyGene oligo factory

  1. 1. EasyGene Oligo Factory Developed by Peter Jepsen Åvej 25 DK-3500 Værløse Ph: +45 42724343
  2. 2. Turn-key Oligo Factory From synthesis to final oligos within a cabinet Synthesis of oligos Cleavage from the support Removal of protection groups Purification of the oligos Dry down oligos
  3. 3. Synthesis of oligos 20 bottle positions  The effective and compact EasyGene Reaction rotor for apparatus defines a new simultaneous synthesis of standard within oligo 24 oligos synthesis Snap-On bottle holders  The apparatus is for quick bottle exchange intelligently constructed, using a minimum of materials and machine Push-On fittings for quick parts maintenance and service  Reduced tubing results Supports different bottle in low death volumes, types accurate delivery of chemicals and reduced Snap-On valves for quick chemical consumption user friendly maintenance Two 24 oligo synthesizers  The apparatus is Green chemistry, due to controlled by user low consumption of friendly and flexible materials and chemicals software
  4. 4. Cleavage from the support Push-On fittings for quick  The oligo is cleaved from maintenance and service the solid phase synthesis support directly from the synthesis rotor into Supports different bottle standard vials types  During the cleavage Snap-On valves for quick process aliquots of user friendly maintenance NH4OH are added to the columns at specified Same spare parts as used time intervals and in the synthesizer incubated at room temperature. The liberated oligo is eluted into vials for further processing. 24 column cleavage apparatus  The apparatus is controlled by user friendly and flexible software
  5. 5. Removal of protection groups  The oligo eluted in NH4OH is incubated at 55oC for fex. 6 hours for removal of protection groups Oven for deprotection
  6. 6. Purification of oligos Push-On fittings for quick  The 24 well rotor is maintenance and service loaded with trityl-on reversed-phase column material Supports different bottle types  The deprotected oligos are added to the wells Snap-On valves for quick and the oligos are user friendly maintenance purified according to the instructions of the Same spare parts as used manufacturer in the synthesizer  In the final step the oligos are eluted into standard vials for further processing. 24 column purification  The purification process is controlled by the EasyGene software.
  7. 7. Dry down oligos  To dry down the oligos the elution buffer from the purification process is evaporated by using heat and a dry air flow  The oligos are now ready for shipment Two 24 column evaporators
  8. 8. Plug and Play  Easy to service  The user can easily change the apparatus  Same software for all machines  Custom design available Easy to service
  9. 9. High quality oligonucleotides  HPLC analysis of a 25- mer oligo produced on the EasyGene apparatus  The analysis shows that only full-length oligo product is present in the oligo preparation. Truncated N-1 oligo versions cannot be observed
  10. 10. EasyGene - Low production expences EasyGene 24 Column  Oligo production on in-house Chemicals Price pr/ml ml/base Price/base Acetronitrile € 0,024 0,25 € 0,0060 24-well rotor Deblock € 0,035 0,15 € 0,0053 Activator € 0,170 0,05 € 0,0085 Amidit € 0,420 0,05 € 0,0210 Cap B € 0,080 0,035 € 0,0028 Cap A € 0,07 0,035 € 0,0026 Oxidizer € 0,07 0,05 € 0,0035 $/base (50 nmol scale) € 0,05 Chemical expenses for a 20 mer oligo € 0,99 Bulk CPG/oligo € 0,05 Total for a 20 mer oligo € 1,04 K&A Laborgeraete 8/16/32 Column  Oligo production on Chemicals Price pr/ml ml/base Price/base commercial single oligo Acetronitrile € 0,024 2 € 0,0480 Deblock € 0,035 0,4 € 0,0140 columns Activator € 0,170 0,09 € 0,0153 Amidit € 0,420 0,05 € 0,0210 Cap B € 0,080 0,075 € 0,0060 Cap A € 0,07 0,075 € 0,0056 Oxidizer € 0,07 0,12 € 0,0084 $/base (50 nmol scale) € 0,12 Chemical expenses for a 20 mer oligo € 2,37 CPG Column/oligo € 1,50 Total for a 20 mer oligo $ 3,87
  11. 11. Oligo prices in Europe From commercial oligo producers Price/base in € Price/oligo in €
  12. 12. Oligo prices in US/Canada From commercial oligo producers Price/base in € Price/oligo in €
  13. 13. EasyGene Oligo Factory “First-Mover” The only solution on the market, offering synthesis and post synthesis processing in one cabinet. The cost of an oligo is reduced to € 0.05 per base High Quality oligos Quick and easy to service Minimum hands-on time Cleantech /Green chemistry • Low chemical consumption • Low energy consumption • Fewer spare parts

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