8. Micro fluidic cell designed for pressure and
diffusion experiments through pipettes.
9. Flow cell designed for visualizing electrical and pressure
driven translocation events through pipettes.
10. Oscillation of 1 micron polystyrene beads in
alternating electric field.
Position A Position B
Polystyrene
bead
Function
generator
11. Calculating the velocity of 1 micron polystyrene
beads oscillating in alternating electric field.
12. 1a 1b 1c 1d
5a 5b 5c 5d 5e
Translocation of Polystyrene beads through
sub micron glass pipettes
Fig (1a-1d) translocation of 15 micron beads through 50 micron pipette
Fig (5a-5e) translocation of fluorescent 15 micron beads through 30 micron pipette
13. 2a 2b 2c 2d
3a 3b 3c 3d 3e
4a 4b 4c 4d
Fig (2a-2d); (3a-3e); (4a-4d) – Translocation of 3 micron beads through 5 micron pipette
14. Pressure driven Translocation of 450 nm polystyrene beads through a 900 nm pipette,
image taken under 40X objective.
15. Pressure driven diffusion through pipettes to track the
trajectory of polystyrene beads at the mouth of the
micropipette
16. Pore diameter: 1.6 µm
Translocation and visualization of polystyrene beads
(500 nm and 1 µm) under alternating electrical stimulus.
Flow cell used for visualizing and performing translocation events.
17. 1 2 3
4 5 6
7 8
Visualizing a single electrical translocation event, of a 1 micron polystyrene bead oscillating
under alternating field at 3V.
