medical laboratory technology

1. Luminometry

2. (for Adding
sample)
Read out device
photomultiplier
Amplifier
syringe
Reaction chamber

3. Luminescence
When electrons are excited it is raised to higher
higher energy level
Then it return back to lower energy level and
emits light.
This phenomenon is called luminescence.
Based on the method of excitation
luminescence is of different types

4. Types
1. Chemiluminescence
2. bioluminescence
3. electrochemiluminescence

5. Chemiluminescence

6. It is the emission of light when an electron
returns from an excited or higher energy
level to a lower energy level.
If the excitation event is caused by a
chemical reaction the luminescence is called
as chemiluminescence.

7. The technique used to measure
chemiluminescence is called
chemiluminometry.

8. It involves oxidation
• Luminol
• Isoluminol
• Luciferin
By an oxidant
• H2O2

9. These reaction occur in the presence of
catalyst such as enzyme
 eg.
• Alkaline phosphatase
• Horseradish – peroxidase

10. Advantages:
• Sensitive
• Low consumption of reagent and high
stability
• Short incubation period

11. Uses
Hormones  insulin, thyroxin, testosterone etc.
Vitamin B12 assay
Tumor marker assay
 β-HCG
C-reactive protein

12. Applications
Chemiluminesence immunoassay
DNA hybridization detection
Western blotting
Forensic science
Food analysis