Final paper of plant bacteriology

1. Plant bacteriology (Total marks 100)
Q.1 Fill in the blanks (1 x10=10 marks)
(1 ) ………………. Isolated and described root nodule bacteria in leguminous
plants.
(2) Causal organism of fire blight of apple and pear is ……………………………
(3) ………………….. is considered as father of plant bacteriology.
(4) First reported plant bacterial diseases in India …………………………….……
and causal organism was isolated at ………………….
(5) Who discover bdellovibrios ……………………..
(6) The first complete sequence of bacterial genome was published by
………………………………………
(7) Spacebetween the outer membrane and inner membrane of bacterial cell is
known as …………………………………….
(8) Some bacteria have an extra circle of genetic material called as
…………………………………..
(9) Bacteria that infect bacteria are known as …………………
(10) Bacterial leaf blight of rice is caused by …………………..
Q.2 Mention True or false for a statement given below- ( 1x10 =10 marks)
1) Pseudomonas is gram positive bacteria.
2) Xanthomonas is gram negative bacteria.
3) Mycoplasma and spiroplasma are the prokaryotes.
4) Citrus canker is caused by virus.
5) Penicillin was isolated from a bacterium.
6) Xylella sp. Is a fastidious bacterial pathogen.
7) Nearly all fastidious vascular bacteria are gram negative.
8) Pseudomonas is the genus which causes maximum number of plant
diseases.

2. 9) Spiroplasma multiply by fission
10) Bacterial scab diseases are caused by species of the genus
agrobacterium.
Ques-3 Match the following ( 1x1 =10) marks
1) Citrus stubborn (a) Xanthomonasaxonopodis p.v citri
2) Citrus canker (b) Spiroplasma citri
3) Crowngall of grapes fruit (c) Agrobacterium tumefaciens
4) Transformation (d) Theory of fermentation
5) Agar –Agar (e) Fire blight of apple and pear
6) Louis pasteur (f) Mycoplasma like organism
7) T.J. burill (g) The first antibiotic
8) Doi et. al (h)The first broad spectrum antibiotic.
9) Penicillin (i) Solidifying agent
10) Streptomycin (j) Griffith
Ques-4 Multiple choice questions. (1 x10=10 marks)
1) L-form of bacteria are –
(a) Pathogenic
(b)Saprophytic
(c)Anaerobic
(d)Cell wall lacking
2) The test which confirms bacterial infection in plants is-
(a) Iodine test
(b)Ooze test
(c)Cobalt test
(d)All of the above
3) Bacterium flagellum is consistof –
(a) Meurin
(b)Glycoprotein
(c)Nuclic acid
(d)Flagellin

3. 4) Which one is a fastidious vascular bacterial pathogen –
(a) Pseudomonas sp.
(b)Erwinia sp.
(c)Xanthomonas sp.
(d)Xylella sp.
5)The symbiotic nitrogen fixing bacteria genus is –
(a) Azatobacter
(b)Rhizobium
(c)Azospirillum
(d)Clostridium
6) In India a schoolof plant bacteriology at the college of agriculture, Pune was
estabilished by -
(a) M.K. Patel
(b)J.P Verma
(c)J.N Chand
(d)P.N. Patel
7) Most plant pathogenic bacteria are –
(a) Gram-negative
(b)Gram-positive
(c)Non flagellable
(d)Cell wall lacking
8) Bacteria are multiply by-
(a) Budding
(b)Parasexuality
(c)Binary fission
(d)Fragmentation

4. 9) Most bacteria below pH 5.5 behave as -
(a) Gram-negative
(b)Gram-positive
(c)Neutral in gram reaction
(d)Polar
10). Most bacteria above pH 11.0 behave as-
(a) Gram-negative
(b)Gram –positive
(c)Neutral in gram reaction
(d)polar
Ques-5. Write down the contribution of the following scientist (2x5=10)
(a) G.C. Ehrenberg
(b)RobertKoch
(c)T.J burill
(d)Woronin
(e) M K patel
Ques.6 Answer the following (4x2=8)
(a) Classification of bacteria according to cell arrangement
(b)Classification of bacteria according to flagellar arrangement
Ques-6. A nswers “Why” ? (3x5 = 15 marks)
A. Why bacterial cells are stained ?
B. Why common scab of potato is favoured by dry soil and checked by wet
soil ?
C. Gram positive bacteria appear deep violet in colour and gram-negative
bacteria appear red in colour during gram staining ?
D. Why the citrus canker pathogen does not survive in soil ?
E. Why phytoplasma are sensitive to tetracycline but resistant to penicillin?
Ques-7. Explain (4x5=20)

5. (a) What is the Scopeofplant bacteriology
(b) Nutrients that are required for the growth of plant pathogenic bacteria.
(c)Classification of organism on the basis of principle carbonand energy
source.
(d) Multiplication in bacteria.
(e) Growth and growth curve
Ques-8 Schematic illustration of lytic cycle and lysogeny (7x1=7)
Answers
1- Woronin (1876)
2- Erwinia amylovora
3- E.F smith
4- Bengal blight of potato , pune
5- H stole (1962)
6- Fisher et al in 1995
7- Periplasm
8- Plasmid
9- Bdello vibrio
10- Xanthomonas oryzae pv oryzae

6. Ques 2.
1- False
2- True
3- True
4- False
5- False
6- True
7- True
8- True
9- True
10- False
Ques 3.
 1-b
 2-a
 3-c
 4-j
 5-i
 6-d
 7-e
 8-f
 9-g
 10-h
Ques-4
1) D
2) B
3) D
4) D
5) B
6) A
7) A
8) C

7. 9) A
10) B