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TRICHODERMA HARZIANUM

12/1/2013

1
 Trichoderma ជាប្រភេទផ្សតមានប្រភោជន៍សំរារ់កំចាត់ផ្សតចង្ប្ៃភ
ិ
ិ
ចចកចំណាត់ថ្នក់ Taxonomy ដូចខាៃភប្ោម៖
ា
រជជៈ

សាខា
ថ្នក់
ា

លំ ដារ់
ប្រួសារ

សណាន
ា

:

Fungi

:

យវាប្តូវបាន
ើ

Sordariomycetes

:
:
:

:

Ascomycota

Hypocreales

Hypocreaceae

Trichoderma

 ភ្ោះវទាសាសាស្តសា Trichoderma harzianum
ម ិ
ប្រេពៈ Heath & Wealth Guardian, 2012។

ររឿម រតនះរ៉ា មី

12/1/2013

2
លក្ខណៈរបស់ Trichoderma



Trichoderma មានចំនួន ៨៩ ប្រភេទ



Colony ររស់វាមានពណបរតង សមានរាងមូលរភោង ឬភប្រម នងមូល
៍
ើ
ិ

ំ ា
ផ្តគ្នភៅកំពូលបន Phiapide មានប្រវែង ៣ភៅ ៥ មប្កូវម៉ែប្ត នងទទង ២
ើ
ិ
ឹ
ភៅ ៤មប្កូវម៉ែប្ត។
ើ



លកខខណរស់ភៅររស់វាភារភប្ចនភៅកាតងបប្ព នងដកសកមម ភ យសតត
ឌ
ើ
ិ ើ
ិ
ើ
ើ

ណភាព ២៥ភៅ ៣០ អងាភស និងសំភនើម ៩០%។
ហ



វាភប្ចនរស់ភៅភលភដើមប្សូែ ជាពភសសប្រព័នធ បនឬសររស់វា នងភៅកាតង
ើ
ើ
ិ
ិ

ដវប្ស។
ើ

ររឿម រតនះរ៉ា មី

12/1/2013

3
សារៈប្រយោចរបស់ Trichoderma

សំ លារ់

ប្រភេទផ្សត
ិ

ទំៃអស់

មនរៃករញ្ហ
ា
ិ
នៃ ភប្រោះ
ិ

ថ្នក់ភផ្សៃៗ
ា
ដល់មនុសស
ររឿម រតនះរ៉ា មី

សាុកទុកបាន
រយៈភពល ១០
ឆ្ ំ ភ
ា

យមាន
ើ

តំង្លភថ្នក

រភៃកនទិនា
ើ

ផ្លររស់

ដំណាំ

12/1/2013

4
 រចនែប្តរ ភរ៉េៃភបាោះ ភមេស ខ្ញី ភប្មច ផ្កង្សៃ ខ្េមបារាំៃ
ក
ា
 ដំណាំពួកសចតក ោភ
ា
ដំឡូៃបារាំៃ ។ល។

េ ចត ប្កូច ភចក ផ្កឈូ ករតា័ ថ្នជក់ អំភៅ
ក
ាំ

 ភប្ៅពភនោះវាជា ភ្នក់ងារជំរុញង្នោរដុោះលូ តលាស់ភលើដំណាំ នៃ
ា
ី
ិ
រចនែ ភោល ររភៃកន នូវសារធាតុ
ើ
ឺ

ចញ្ចាម មប្កូធាតុ នៃផ្ូស័រ។
េ
ិ
ី
ិ

ររឿម រតនះរ៉ា មី

12/1/2013

5
Trichoderma Increasing
► ឧរករត
៍

• ថៃ់បាសេិច
ែ

Rice Cooker

• អៃក ឬចុៃអៃករ
• Trichoderma
• ជញ្ជ ី ៃ

• មជុល

Rubber&Needle

• ភៅសូ កៃ

Water
ររឿម រតនះរ៉ា មី

Broken-milled rice or rice

• ឆ្ៃដាំបាយអរគសនី
ាំ

Plastic bag size 8x12 inch
12/1/2013

6
ររឿម រតនះរ៉ា មី

12/1/2013

7
 ការចំអនបាយពាក់កណ្តលឆ្អន នងពាក់កណ្តលភ
្
្
ិ
ិ
ិ
 ដាក់អងករ ៣ ភារ ោយជាមួយទក ២ ភារ(ប្រសនភរអងកររង)
ឹ
ិ
ើ
ឹ
 ដាក់អងករ ២ ភារ ោយជាមួយទក ១ ភារ(ប្រសនភរអងករមនរង)
ឹ
ិ
ើ
ិ ឹ

 ភយងភ្វការចំអនបាយភដាយភប្រឆ្ងអរគសនើ ៦០០ប្កាម បនអងករ
ើ
ើ
ិ
ើ ាំ
ើ
ោយជាមួយទក ០.៥ លប្ត, ភយងអាចទទួលបាន ១ រ.ប្ក បនបាយ
ឹ
ើ
ើ
ពាក់កណ្តលឆ្អន នងពាក់កណ្តលភ ។
្
្
ិ
ិ

ររឿម រតនះរ៉ា មី

12/1/2013

8
រភរៀរប្ចកបាយដាក់ថៃ់
ដាក់វាកាុៃថៃ់ ភពលវាកំពុៃភៅ
ា

ដាក់បាយ ២៥០ប្ោម/ថៃ់

ររឿម រតនះរ៉ា មី

១ រ.ប្ក ង្នអៃករដាក់កុ ៃ ៤ ថៃ់
ា
12/1/2013

9
រត់ថៃ់បាសេិច ភ
ែ

ររឿម រតនះរ៉ា មី

យរៃ់ចារ ូតដល់បាយភៅអនៗ
ំ
ា ុ
ើ

12/1/2013

10
ភរាយវា ២ ដៃកាុៃមួយថៃ់

ភ្េោរភចាោះប្រភោៃភៅភលើរររដរចដល
ំ
ើ
ងាយប្សួលកាុៃោរចាក់ចលៃ
ំ
ររឿម រតនះរ៉ា មី

១ ដរ អាចដាក់បាន ៣២ ភៅ ៤០ ថៃ់។
12/1/2013

11
ភរាយវាពភលើបាយភអាយភៅចំកណាលបាយកាុៃ
ា
ី
ថៃ់បាសេិចរួចប្ចរល់វាឲ្យសពេ
ែ

ររឿម រតនះរ៉ា មី

12/1/2013

12
ចៃវាជាមួយភៅសូ កៃភអាយជារ់

ររឿម រតនះរ៉ា មី

12/1/2013

13
ភ្េោរប្ចរល់បាយជាមួយTrichoderma ភអាយសពេ
ើ

ររឿម រតនះរ៉ា មី

12/1/2013

14
ភប្រប្បាស់មុ លកាុៃោរភចាោះរុនធ ភៅភលើកញ្ច រ់ ១៥ ភៅ ២០ កចនែៃ/ថៃ់
ជ
ើ

ររឿម រតនះរ៉ា មី

12/1/2013

15
 ទកចនែៃថៃ់បាយ កាុៃទកចនែៃចដលរារ់ភសមើ(មន ប្តួតជាន់ភលើរ)
ា
ី
ិ
ី
 មានពនែឺ រ៉េចនា មនចមនពនែប្ពោះអាទតយ
ុ
ិ
ឺ
ិ

ររឿម រតនះរ៉ា មី

12/1/2013

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ររឿម រតនះរ៉ា មី

12/1/2013

17
ររឿម រតនះរ៉ា មី

12/1/2013

18
រន្ទរ់ពចាក់ចលៃបានរយៈភពល ២ ង្ថៃ
េ
ំ
ី

ររឿម រតនះរ៉ា មី

12/1/2013

19
ភ្េោរប្ចរល់បាយមាៃភទៀតភប្ោយដាក់ Trichoderma បានរយៈភពល២ ង្ថៃ
ើ

ររឿម រតនះរ៉ា មី

12/1/2013

20
រន្ទរ់ពដាក់ Trichoderma រយៈភពល ៧ ង្ថៃ
េ
ី

ររឿម រតនះរ៉ា មី

12/1/2013

21
ររឿម រតនះរ៉ា មី

12/1/2013

22
ការប្រយ័ត្ប្រយយង
ន
យប្រើប្ាស់ឆ្ងោំាបាយអគ្គសនខ្យី
ន ំា
ី

ោក្់វាក្នងយេលដែលវាយត
ៅ
ុ

ទុក្វាក្នងបន្ទប់យោយគ្មនខ្យល ល់
ា
ា
ុ

យ្វយោយមានខ្យប្រយោងយលក្ក្ញ្ច ប់ាសាច ១៥ យៅ ២០ រុនខ្យធ
ា ិ
ើ
ើ
ទុក្វាក្នងបន្ទប់
ា
ុ

ប្សបល់វាបន្ទប់ោក្់ Trichoderma រយៈយេល ២ ថ្ងៃ
ា
ររឿម រតនះរ៉ា មី

12/1/2013

23
 រភរៀរង្នោរភប្រប្បាស់ Trichoderma
ើ

បាញ់ ភលើរចនែ
ប្សរល់ជាមួយទក ភ
ា

យបាញ់ ភៅភលើរកជាតទៃអស់
ុ ខ
ើ
ិ ំ

(១ រ.ប្ក Trichoderma: ២០០ លីប្តទក)
ា

បាញ់ វាកាុៃភពលប្ពក ឬ ភពលលាច មនភៅ
ៃ
ា
ា
ិ

ររឿម រតនះរ៉ា មី

12/1/2013

24
ការបប្រើប្ាស់
Trichoderma
កាុៃមួយប្ោម 3 x106 colony
ោរភប្រើប្បាស់វាមាន ៧ រភរៀរដូចជា៖
Trichoderma ចំនន ៦ភៅ១០ ប្ោម/ទក ១លីប្តចំភោោះប្ររ់ពូជមួយរទបូប្ោម
ួ
ា
ី

២៥០ ប្ោម/

ចតាលាយជាមួយទកប្ចរល់ ជាមួយប្ររ់ពូជផ្ករ់រយៈភពល ២៤
ា
ិ
ា

ភមាៃ រយៈភពល ៥ភៅ១០ ភមាៃ
៉េ
៉េ

ោរភប្រភលើប្ររ់Trichodermaចំនន ៦ភៅ១០g លាយជាមួយទកមួយលីប្តចំភោោះ
ួ
ើ
ា
ប្ររ់ពូជ ១kg ភ

យោរភប្រភនោះ រមុនភពលចដលសារ។
ើ
ើ
ឺ

ោរភប្រភលើថ្នល Trichodermaចំនួន ១០ ភៅ ២៥g លាយជាមួយ ទកមួយលីប្ត
ា
ើ
ា
ចំភោោះង្ផ្េដី ១០០ចម៉េប្តោភរ។
៉េ
ររឿម រតនះរ៉ា មី

12/1/2013

25
ោរភប្រភលើកូនសំ ណារ Trichodermaចំនួន ១០g លាយជាមួយ ទកមួយលីប្តប្តាំកូនសំ ណារ
ើ
ា

 រយៈភពល ១០ន្ទទី មុនភពលសេុៃ។
ោរភប្រភលើដដាដុោះោរភប្រប្បាស់ភមៅ Trichodermaសុ ទធចំនួន ៥kg កាុៃមួយ
ី ំ
ើ
ើ

កតាភប្ោយបាច
ិ

រួចប្តូវេជួរប្តឡរ់។ ម៉េាៃភទៀតភយៃអាចភប្រប្បាស់ភមៅTrichodermaចំនួយ ១kg លាយជាមួយ
ើ
ើ
ជីកំរុសា ឬលាមកសតេចំនួន ១០០ kg ភដាយប្ររទុករយៈភពល ៧ ង្ថៃ ភ
៉េ

យ៣ ភៅ ៤ ង្ថៃភប្ោយ
ើ

ប្តឡរ់មាៃ រួចអាចយកភៅបាចកាុៃចប្សបាន។

ោរភប្រភលើរកខជាតមុខ្ដាក់ភមៅTrichoderma ភៅភលើដចកបរភដមភឈើ ប្តូវភ្េដភៅជតភដមភអាយ
ុ
ី
ើ
ិ
ើ
ើ
ិ
ើ ី
មានសំ ភតមជាមុនសិនចាំភប្រភមៅ Trichoderma ចំនួន ១០g លាយជាមួយទកមួយលីប្ត។
ើ
ើ
ា

ររឿម រតនះរ៉ា មី

12/1/2013

26
ការលាយ Trichoderma

ររឿម រតនះរ៉ា មី

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27
ររឿម រតនះរ៉ា មី

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28
ប ើម្បើបប្រើប្ាស់Trichoderma ក្នងប្រព័ន្បប្ចសប្សព
ធ
ុ

Trichoderma ១ រ.ប្ក/ទក២០០លីប្ត
ា

រចនែមទក ភ
ា
ររឿម រតនះរ៉ា មី

យប្សរល់វាភអាយសពេ
ើ

12/1/2013

29
រចនែមទកង្នសៃ័រររស់Trichoderma(1kg)/200L of
ា
ររឿម រតនះរ៉ា មី

Water

12/1/2013

30
Add fish fermentation 0.5L/200L of Water

ររឿម រតនះរ៉ា មី

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31
ប្សរល់វាភអាយសពេ ឬ កូវាភអាយសពេលា
ររឿម រតនះរ៉ា មី

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32
រភរៀរង្នោរតភ្នរ់ជាមួយប្រព័នទក
ជ
ធ ា

្ុៃទកចដលបានប្សរល់ Trichorderma
ា

ប្សោះទក
ា

រំពៃទកភប្សាចប្សពេ
ា
ររឿម រតនះរ៉ា មី

រំពុៃរតទកភចញពប្សោះ
ឺ ា
ី
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33
ំ
រំពៃ់ទកភប្សាចប្សពេភៅភលើដណាំ
ា

រំពៃ់រតទកភចញពប្សោះ
ឺ ា
ី
រំពុៃចដលតភ្នរ់ភចញព្ុៃ Trichoderma
ជ
ី

ររឿម រតនះរ៉ា មី

12/1/2013

34
របបៀបនន្ការបប្រើប្ាស់ជាម្ ួយប្ាប់(Seed Treatment)

១០ ប្ោម (Trichoderma)/1kg(Seed)

ររឿម រតនះរ៉ា មី

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35
ររឿម រតនះរ៉ា មី

12/1/2013

36
បប្រើប្ាស់ប ម្បើលាយជាម្ ួយលាម្ក្សត្វ
ើ
(Trichoderma Compost)

លាមកសតេ, កំរសា ១០០ រ.ប្ក
ុ៉េ

Trichoderma 1kg

កនេក់ ៤ រ.ប្ក
ភប្រប្បាស់ ៥០ ភៅ ១០០ប្ោម/ម២
ើ

ររឿម រតនះរ៉ា មី

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37
ររឿម រតនះរ៉ា មី

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38
Use to mix with planting
Material(Medium)
Planting Material(Medium) 4 parts

Trichoderma Compost 1 part

ររឿម រតនះរ៉ា មី

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39
Trichoderma Compost 1 part

Medium 4 parts

ររឿម រតនះរ៉ា មី

Sowing seed

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40
Fungus that Trichoderma can
control

Anthracnose of Pepper

Southern leaf blight
of maize

Wet root/Root rot

ររឿម រតនះរ៉ា មី

Leaf blight Phytophthora Anthracnose of Mangos

12/1/2013

41
Wilt rot on stem

Eggplant

Sweet Basil
Hot pepper
ររឿម រតនះរ៉ា មី

12/1/2013

42
Root Rot Disease

Bean Plant

Asparagus

Duran
Orages

Orchid
ររឿម រតនះរ៉ា មី

Eggplant

12/1/2013

43

Orage
ស ូម្បញ្ញក្់
ា

• ភពលភប្រប្បាស់ោមភប្រថ្នសំលារ់ផ្សត ៤ភៅ៥ ង្ថៃ
ើ
ើ ាំ
ិ

• ោមភប្រភពលដីរនសំ ភតម
ម
ើ
ើ

•ោមដាក់ប្ររ់ពូជប្រលាក់ជាមួយភមៅTrichoderma
ភ

យយកភៅដាក់រោះង្ថៃ
៉េ
ើ

ររឿម រតនះរ៉ា មី

12/1/2013

44
Materials of Compost
Pig Manure 40%
Cow Manure 40%
Rice Husk 10%

DM of Lead Tree 10%
Water 60-80L(Fish Fermentation 1L+10L of Water
ររឿម រតនះរ៉ា មី

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45
How to mix Compost
Put Pig,Cow Manure, Rice Husk and Lead Tree
Turn on Machine
Add water in successive time till finish
About 20 mn after mixing we can bag it

Bag it and keep it in uncover 30days can use it
Use it 1.25kg-1.90kg(Best) for 1 sq meter
ររឿម រតនះរ៉ា មី

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46
ររឿម រតនះរ៉ា មី

12/1/2013

47
Fish Fermentation
I. Materials (first way)

•
•
•
•
•

Fish 1 kg
Molasses 1 kg
EM 1 liter
Do not add water
Use mostly in spraying on
crop

II. Materials (second way)

•

Fish 30 kgs

•

Molasses 1 kg

•

EM 1 liter

•

Water 10 liters

•

Use mostly in mixing with
Compost

ររឿម រតនះរ៉ា មី

12/1/2013

48
How to soak FF(Both Materials)
Put all material in Tank
Cover it (but not strong)
Mix it everyday
After 30 days we can use it
ររឿម រតនះរ៉ា មី

12/1/2013

49
After incubating 30days
(Smell is good)

ររឿម រតនះរ៉ា មី

12/1/2013

50
How to Use FF
20 cc(FF)+20L(Water) to spray on crop
(First Way)
1L(FF)+ 10L(Water) mix with compost
(Second Way)
Can spray on every crop(mostly in vegetable
and fruit)
Benefit is to encourage growth of plant and
increase yield and flower and good
decompose for Compost
ររឿម រតនះរ៉ា មី

12/1/2013

51
How to Make Fruit Hormone
Fruit 40kg( Pineapple ,Papaya ,..….)
Mollase 10kg
Mix it together, 7 days after incubating we
can use it

40 cc(Fruit Hormone)+40L(Water)
Benefit: It can encourage plant’s growth
,flowers, bio-hormone
ររឿម រតនះរ៉ា មី

12/1/2013

52
ររឿម រតនះរ៉ា មី

12/1/2013

53
ររឿម រតនះរ៉ា មី

12/1/2013

54

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Ramy Trichoderma 2014 Cambodia