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Humoral immune response
1. Immune Response I
DR. SUFI H. Z. RAHMAN
MBBS, MD (IMMUNOLOGY)
LECTURER, MEDICAL FACULTY, AUCMS
2. Objectives
âMechanism of non-specific immunity
âProcess of phagocytosis
âConcept of specificity, immunological memory and
non-self
âInduction process of B lymphocytes in forming
antibodies
âCharacteristics of different classes of antibodies and
functions of each class
âPrimary and secondary antibody response to antigens
3. Immune System
âOrgans, cells and molecules the protect the
host from infections and cancers
âTwo different but coordinated immune
systems are functioning to protect the host
4. Immune System
Innate (inborn or non-specific) Immune System
First line of defense
Prevent microbial attachment, colonization and entry
Disposes pathogens rapidly and nonspecifically if they can
enter
Adaptive (acquired or specific) Immune System
Second line of defense when the innate system fails
Devotes specialized sets of cells for each pathogen
to identify, mark for disposal and retaining memory for
future
5. Comparison between
Innate and Adaptive Immunity
Attribute Innate Immunity Adaptive Immunity
Activation Active prior to exposure
exposure to any
microbe or antigen
Activated by exposure
exposure to microbes or
or antigens
Lag phase Absent
Response is immediate
Present
Response takes few
days
Specificity Limited
Targets all pathogens
High
Targets specific
pathogen
Memory Absent
Same response in 1st
Present
Amplified response in
6. Organization of the Immune System
Innate
Anatomical barrier
Physiological barriers
Cellular barrier
Inflammatory barrier
Adaptive
Organs
Cells
Molecules
7. Innate Immunity
âSkin
⢠Epidermis acts as mechanical
barrier and retards entry of
microorganisms
⢠Acidic environment (pH 3- 5)
retards growth of microorganisms
âMucous membrane
⢠Normal microbial flora compete
with pathogenic microorganisms
for attachment and nutrients
⢠Mucus entraps foreign
microorganisms
⢠Cilia of surface epithelium propel
microbes out of the body
Anatomical Barrier
8. Innate Immunity
âFever response
Inhibit growth of microorganisms
âAcidic environment
Stomach: Kills microorganisms
Vagina: Retards growth of microorganisms
âChemical mediators
Lysozyme of tear: Cleaves bacterial cell wall
Interferon: Induces antiviral state in uninfected
cells
Complement: Lyses microbes or facilitate their
phagocytosis
Antibacterial peptides: Kill pathogenic bacteria
Physiological Barrier
9. Innate Immunity
âPhagocytic cells
⢠Neutrophils
⢠Monocytes and Macrophages
⢠Dendritic cells
⢠Eosinophils (?)
Detect Pathogen associated molecular pattern
(PAMP) on the surface of microbes by their Pattern
recognition receptors (PRR), internalize
(phagocytose) and kill them
âNon-phagocytic killer cells
⢠Natural killer (NK) cells
Induce apoptosis of virus infected and tumour cells
Cellular Barrier
10. Innate Immunity
âInflammatory response upon tissue damage
or microbial infection induces leakage of
vascular fluid containing antibacterial
peptides to the site of damage or site of
entry of microbes
âInflammatory response also causes influx of
phagocytic cells (neutrophils, monocytes and
macrophages) to the affected site
âAntibacterial peptides and phagocytes then
destroy the microbial agent
Inflammatory Barrier
12. Innate Immunity
âPhagocytic cells recognize PAMPs
on microbial surface by their PRRs
âExtend pseudopodia and
internalize the microbes in
phagosome
âKill microbes by
⢠Oxygen dependent mechanism
Respiratory burst in the
phagosomes produce reactive
oxygen and reactive nitrogen
intermediates that are extremely
toxic to microbes
⢠Oxygen independent
mechanism
Phagosomes fuse with lysosomes
and lysosomal enzymes kill
microbes independent of oxygen
Phagocytosis
14. Killing by NK cells
Innate Immunity
âBind to cells (normal, infected or tumour
cells) by Activation receptor (AR) that provide
activation signal to kill the cell
âAlso bind to Class I MHC molecules on
normal cells by Killer cell inhibitory receptors
(KIR) that provide inhibitory signal
âInhibitory signal is stronger than activation
signal and normal cells are not killed
âAs virus infected cells and tumour cells
reduce Class I MHC molecule expression, they
cannot provide inhibitory signal and are killed
by NK cells
âNK cells induce apoptosis of virus infected
cells and tumour cells
16. Adaptive Immunity
âDevelops after exposure to pathogen or
antigen
âAdaptive immunity has the following
attributes
1. Antigenic specificity
2. Diversity
3. Immunologic memory
4. Self/ non-self recognition
17. Adaptive Immunity
âAntigenic specificity
⢠Capable of recognizing and selectively eliminating
specific foreign microorganisms and molecules (i.e.
foreign antigens)
⢠Can distinguish subtle differences among antigens
âDiversity
⢠Can generate tremendous diversity in recognition of
molecules
⢠Can recognize billions of unique structures on foreign
antigens
⢠This ability is generated by recombination of
Immunoglobulin and T cell receptor (TCR) gene segments
⢠The immune system is capable of generating more than
109 clones of B cells and 1010 clones of T cells, only a
small fraction of which is displayed at any time
⢠Each clone is specific for one epitope (antigen)
18. Adaptive Immunity
âImmunologic memory
⢠Exhibits memory of an antigen to which it is
exposed
⢠A second exposure to the same antigen induces a
heightened state of immunological reactivity
⢠Because of this attribute, adaptive immune system
can confer life-long immunity to many infectious
agents after an initial encounter
âSelf/ non-self recognition
⢠Capable of distinguishing self from non-self
⢠Normally responds only to foreign antigens
⢠This is due to development of self tolerance during
maturation of B and T lymphocytes
19. Adaptive Immunity
Adaptive immune system has two arms
Adaptive Immunity
Humoral Immunity Cell mediated Immunity
⢠Provided by B lymphocytes
⢠Can recognize protein,
polysaccharide, phospholipid and
nucleic acid antigens
⢠Can act against soluble or free
antigens
⢠Provides immunity to
extracellular bacteria, viruses and
toxins
⢠Causes Type I, II & III
hypersensitivity
⢠Provided by T lymphocytes
⢠Can recognize only protein
antigens
⢠Recognizes antigens presented
by APCs with Class I or Class II
MHC molecule
⢠Provides immunity to
intracellular bacteria, viruses,
fungi and protozoa
⢠Causes Type IV hypersensitivity
⢠Causes acute graft rejection
20. V= Variable, D= Diversity, J= Joining segment of Ig gene, H= Heavy chain, L= L
B cells
Humoral Immune Response
Maturation/ Ontogeny
21. Humoral Immune Response
âMature B cells express membrane bound immunoglobulin
(mIg)
âNaĂŻve B cells express mIgM and mIgD on their surface
âmIgM acts as antigen receptor called B cell receptor (BCR)
âEach B cell contains 105 BCRs, all specific for a single
epitope
âEach clone of B cell is specific for a single epitope
â109 clones of B cells can recognize 109 epitopes
B cells
22. Humoral Immune Response
âMature B cells circulate in the blood and lymph and are
carried to the secondary lymphoid organs e.g. lymph
nodes and spleen
âAntigens are also carried by blood or lymph to the
secondary lymphoid organs
âB cells usually meet their specific antigens in these organs
âAn antigen binds to a B cell (or a clone of B cells) which
carries mIgM specific for that antigen and activates it
(clonal selection)
âB cell activated by an antigen starts to proliferate and
increase number of cells (clonal expansion)
â After expansion, the cells differentiate to plasma cells and
memory B cells
B cell activation
23.
24. Humoral Immune Response
Binding of a
TI antigen with mIgM
on B cell surface
activates the B cell
specific for it
B cell activation by TD and TI B cell antigens
Binding of a TD antigen with mIgM on
B cell surface provides stimulatory signal (1)
Antigen is internalized & presented to TH
cell
CD40 on B cell also bind to CD40L on TH
cell that provides costimulatory signal (2)
These two signals and cytokines from TH
cell activates the B cellTD= TH cell dependent, TI= TH cell independent
26. Humoral Immune Response
âPlasma cells secrete antibodies
âSecreted antibodies have the same
specificity as the mIgM on the surface of
B cell from which the plasma cell is
derived
âAntibodies are the effector molecules of
humoral immune response that bind with
the antigen and eliminate the microbe
âInitially plasma cells secrete IgM antibody
âWithin a few days antibody class
switching occurs and they secrete IgG (or
IgA or IgE)
âMemory B cells have a long life span and
provide heightened immune response if
the person encounters the same antigen
in future
Antibody production
27. Humoral Immune Response
Antigen Elimination
Antibodies eliminate antigens by
1. Neutralization of toxins and
viruses
2. Complement activation followed
by
a. Cytolysis
b. Opsonization and
phagocytosis
c. Immune complex clearance
3. Antibody dependent cell
mediated cytotoxicity (ADCC)
28. Humoral Immune Response
Antigen Elimination
Antibody Dependent Cell Mediated Cytotoxicity (ADCC)
Cells that have receptor for Fc
portion of IgG (Neutrohils,
Macrophages and NK cells) or IgE
(Eosinophils) induce apoptosis of
the infected cells with bound IgG or
IgE by:
⢠Neutrophils: Lytic enzymes
⢠Macrophages: Lytic enzymes,
TNF
⢠Eosinophils: Lytic enzymes,
perforins
29. Humoral Immune Response
Antibody
â Antibodies are antigen binding proteins present on B
cell membrane and secreted (immunoglobulins) by
plasma cells
â Membrane-bound antibody confers antigenic
specificity to B cells; antigen-specific proliferation of
B-cell clone is elicited by the interaction of
membrane-bound antibody with its specific antigen
â Secreted antibodies circulate in the blood, where they
serve as the effectors of humoral immunity by
searching and neutralizing antigens or marking them
for elimination
â All antibodies share structural features, bind to
30. Humoral Immune Response
Antibody Molecule
â Consists of 4 peptide chains
⢠2 identical heavy (H) chains (MW 50 kD)
⢠2 identical light (L) chains (MW 25 kD)
â Each L chain is bound to a H chain by
disulfide bond and form 2 H-L
combinations
â H-L combinations are held together by
disulfide bond between H chains
â Amino acid sequence in amino terminal
is variable ( V region) and form the
antigen binding site (hypervariable
region or CDR)
â Amino acid sequence in carboxyl
terminal is relatively constant (C region
or Fc) and is responsible for biological
function e.g. complement fixation,
placental transfer, opsonization etc.
MW= Molecular weight, kD= kilo Dalton,
CDR= Complementarity determining
region
Fc= Fragment crystallizable
31. Humoral Immune Response
There are 5 types of H chains: g, d, a, m and e and 2 types of L chains: k and l
Both H and L chains have domain structure, 4- 5 domains in H and 2 domains in L
chains
An antibody molecule contains only one type of H chain and one type of L chain
Depending on the type of H chain, antibodies are classified in to IgG, IgD, IgA, IgM and
IgE
Antibody
32. Humoral Immune Response
âIgG contains g H chain and is a monomer
IgG has 4 subtypes IgG1, IgG2, IgG3 and IgG4
âIgD contains d H chain and is a monomer
âIgE contains e H chain and is a monomer
âIgA contains a H chain and is a dimer where
each monomer is joined together by a J chain
and may contain a secretory component
IgA has two subtypes: IgA1 and IgA2
âIgM contains m H chain and is a pentamer
where monomers are joined together by
disulphide bonds and J chain
Antibody Types and Subtypes
These types are called isotypes because they are same in
all members of the same species (cf. allotype and
idiotype)
34. Humoral Immune Response
âPrimary immune response
Immune response that
occurs after 1st exposure to
an antigen
âSecondary immune
response
Immune response that
occurs after 2nd or
subsequent exposure to the
same antigen
Primary and Secondary Immune Response
35. Humoral Immune Response
Primary and Secondary Immune Response
Attribute Primary response Secondary response
Antigen type Both T dependent and T
and T independent
Only T dependent
Responding cells NaĂŻve B or T cells Memory B or T cells
Lag period Longer (4- 7 days) Shorter (1- 3 days)
Peak response Occurs in 7- 10 days Occurs in 3- 5 days
Magnitude Low High (100- 1000x)
Antibody isotype IgM predominates IgG predominates
Antibody affinity Lower Higher
36. Humoral Immune Response
Primary and Secondary Immune Response
Primary immune
response
IgM is produced first
then class switch to IgG
Secondary immune response
IgM and IgG are produced
simultaneously from the
beginning with predominant
IgG
39. Cooperation between
Innate and Adaptive Immunity
âAdaptive immunity is not independent of innate
immunity
âThe phagocytic cells crucial to nonspecific immune
responses are intimately involved in activating the
specific immune response
âVarious soluble factors produced by a specific
immune response have been shown to augment the
activity of these phagocytic cells
âThrough the carefully regulated interplay of adaptive
and innate immunity, the two systems work together
to eliminate a foreign invader
40. Cooperation between
Innate and Adaptive Immunity
Component Innate Immune System Adaptive Immune System
Macrophages Phagocytosis and killing of
microorganisms
Presentation of antigens of
phagocytosed organisms to T
lymphocytes, ADCC
Dendritic cells Phagocytosis and killing of
microorganisms
Presentation of antigens of
phagocytosed microorganisms to
microorganisms to T lymphocytes
Complements Activation by
Alternative or Lectin pathway
Activation by Classical pathway
pathway
Neutrophils Phagocytosis of microbes Killing of microbes by ADCC
Eosinophils Phagocytosis of microbes (?) Killing of parasites by ADCC
NK cells Killing virus infected & cancer
cells
Killing of microbes by ADCC
41. Further Review
âLevinson W. Review of Medical
Microbiology and Immunology. 11th edition.
McGraw Hill, 2008.
âKindt TJ, Goldsby RA, Osborne BA. Kuby
Immunology. 6th ed. WH Freeman, 2006.
âAbbas AK, Lichman AH. Basic Immunology.
3rd edition. Elsevier, 2011.